Confirmation of root-knot nematode resistant gene Rmi1 using SSR markers

Musarrat Ramzan, Riffat Ahmad, Naheed Kauser, Anis Ali Shah, Rabia Saba, Iqtidar Hussain, Shahina Fayyaz, Saifullah Khan

Abstract


Background: The Root Knot Nematode (RKN) is a serious economic threat to various cultivated crops worldwide. It is a devastating pest of soybean and responsible to cause severe yield loss in Pakistan. The cultivation of resistant soybean varieties against this pest is the sustainable strategy to manage the heavy loss and increase yield. There is an utmost need to identify RKN resistant varieties of soybean against cultivated in Pakistan. The presented study is an attempt to identify and confirm the presence of resistant gene Rmi1 in soybean. 

Method: Molecular studies have been done using Simple Sequence Repeat (SSR) marker system to identify resistant soybean varieties against Root Knot Nematode (RKN) using fifteen (15) indigenous cultivars and four (4) US cultivars. DNA was isolated, purified, quantified and then used to employ various SSR markers. The amplified product is observed using gel documentation system after electrophoresis.  

Results: Diagnostic SSR markers Satt-358 and Satt-492 have shown the presence of Rmi1 gene in all resistance carrying genotypes. Satt-358 amplified the fragment of 200 bp and Satt-492 generated 232 bp bands in all resistant genotypes. This study confirmed the Rmi gene locus (G248A-1) in all internationally confirmed resistant including six (6) native varieties.

Conclusion: These investigations have identified six (6) resistant cultivars revealing the effective and informative sources that can be utilized in breeding programs for the selection of RKN resistance soybean genotypes in Pakistan. 


Full Text:

PDF

References


Ha B-K, Bennett JB, Hussey RS, Finnerty SL, Boerma HR. Pedigree analysis of a major QTL conditioning soybean resistance to southern root-knot nematode. Crop Science, (2004); 44(3): 758-763.

Tamulonis J, Luzzi B, Hussey R, Parrot W, Boerma H. DNA markers associated with resistance to Javanese root-knot nematode in soybean. Crop Science, (1997); 37(3): 783-788.

Panaud O, Chen X, McCouch S. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Molecular and General Genetics, (1996); 252(5): 597-607.

Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, et al. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics, (2000); 100(5): 697-712.

Gutierrez M, Patto MV, Huguet T, Cubero J, Moreno M, et al. Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops. Theoretical and Applied Genetics, (2005); 110(7): 1210-1217.

Luzzi B, Boerma H, Hussey R. Resistance to three species of root-knot nematode in soybean. Crop Science, (1987); 27(2): 258-262.

Tamulonis J, Luzzi B, Hussey R, Parrott W, Boerma H. RFLP mapping of resistance to southern root-knot nematode in soybean. Crop Science, (1997); 37(6): 1903-1909.

Doyle JJ. Isolation of plant DNA from fresh tissue. Focus, (1990); 1213-15.

Cregan P, Jarvik T, Bush A, Shoemaker R, Lark KG, et al. An integrated genetic linkage map of the soybean genome. Crop Science, (1999); 39(5): 1464-1490.

Li Z, Jakkula L, Hussey R, Tamulonis J, Boerma H. SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode. Theoretical and Applied Genetics, (2001); 103(8): 1167-1173.

Meehan D, Xu Z, Zuniga G, Alcivar-Warren A. High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus) vannamei [Crustacea: Decapoda]. Marine Biotechnology, (2003); 5(4): 311-330.

Rohlf F. NTSYS-PC 2.02. Numerical taxonomy and multivariate analysis system Applied Biostatistics, Setauket, New York, (1998).


Refbacks

  • There are currently no refbacks.