Therapeutic potential of stem cells derived factor-1 alpha (SDF-1α) for skin burn injuries

Nadia Wajid, Azib Ali, Fatima Ali, Noreen Latief, Aamer Qazi


Background: Stem cells derived factor-1 alpha (SDF-1α) enhances the migration of bone marrow cells towards wound hence improve the healing process. The present study focuses on the therapeutic potential of SDF-1α and bone marrow derived cells from rats after SDF-1α treatment for skin burn.

Methods: SD rats were given thermal skin burn. Animals were divided into three groups i.e. SDF (S) with subcutaneous SDF-1α treatment after burn, Burn (B) and control (C). After seven days of treatment rats were sacrificed and skin, liver, heart, kidneys and blood were taken for histological and serological analysis. CBC, serum electrolytes, liver functions tests, renal functions tests, lipid profile, blood glucose as well as ELISA for VEGF, CRP and oxidative stress were performed. Cells were extracted from bone marrow and were transplanted in the burnt animals either directly or after culturing (Bone marrow stromal cells; BMSCs).

Results: A prominent role of SDF-1α on skin thermal wound healing was observed. Importantly the treatment has no significant impact on vital organs like liver, heart and kidney as well as physiological parameters. Lowered inflammation and enhanced angiogenesis was observed by ELISA for CRP and VEGF. BMSCs from SDF group showed higher growth (viability and proliferation), lowered cell death (LDH release), high angiogenesis (VEGF) and decreased oxidative stress. These BMSCs healed the burnt skin better than whole bone marrow of the same group after transplantation.

Conclusion: It may be concluded that SDF-1α can be a potential therapeutic agent for thermal skin burns and BMSCs after the treatment get higher growth and healing potential.

Keywords: Skin; Burn; CXCR-4; SDF-1α; BMSCs

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