Evolution of Phosphoenolpyruvate carboxylase encoding transcripts in Chickpea (Cicer arietinum L.)

Syed Rehmat Ullah Shah, Muhammad Rashid, Muhammad Abuzar Jaffar, Muhammad Aslam, Hinna Shah, Ghulam Rasool


Background: Phosphoenolpyruvate carboxylase (PEPC; EC is an important enzyme encoded by a gene family of at least 2-8 plant type and 1-2 bacterial type genes depending upon genome size or species complexity. This enzyme functions as catalyst for the β-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate in cytoplasm. It is involved in carbon fixation and various other plant metabolic pathways.

Methods: In this study we characterized the evolutionary perspective of PPC transcripts and their abundance pattern in different plant tissues of chickpea (Cicer arietinum L.).

Results: The current study revealed that PEPC enzyme in chickpea is encoded by a gene family of at least 6 transcripts. All active site residues of C3 PEPCs were found in transcripts. Phylogenetic analysis of the amino acid sequences showed two major groups PTPC and BTPC from different ancestral lineages. Divergence of PTPC in two groups and further convergence within species was found in most of the plants while multiple evolutionary divergences was likely to be specific in legumes including chickpea.

Conclusion: CaPPC genes are regulated under various abiotic stress. Furthermore, the expression pattern of the identified genes can be helpful to explore plant metabolism of chickpea under abiotic stresses, which can be the next step to explore more into this gene family in chickpea.

Keywords: Phosphoenolpyruvate carboxylase transcripts; Chickpea; Phylogeny

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