2024-03-29T13:39:57Z
https://submission.als-journal.com/index.php/ALS/oai
oai:ojs.submission.als-journal.com:article/1901
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
Assessing the Awareness, Perceptions, and Behaviors Regarding the Harmful Effects of Smoking Among Smokers – An Interventional Community-Based Study
Dharmarajlu, Santhi Muttipoll
College of Nursing, Jazan University, Jazan. https://orcid.org/0000-0003-0281-1731 http://orcid.org/0000-0003-0281-1731
Dailah, Hamad Ghaleb
Research and Scientific Studies Unit, College of Nursing, Jazan University, Jazan 45142
MD, Anuratha
PSG Hospitals, Coimbatore, Affiliated with The Tamil Nadu Dr. MGR Medical University, Chennai http://orcid.org/0009-0006-9417-8775
Health and Epidemiological studies
Background: Tobacco use is the leading preventable cause of premature deaths. Tobacco control remains a top priority, and health warning labels (HWLs) are one of the recommended methods. The goal of health education is to influence their perception of tobacco use, thereby discouraging smokers and would-be-smokers from smoking. This study is aimed at ascertaining the level of awareness, perceptions, and behaviors regarding the harmful effects of smoking among male smokers in Farasan Island, Kingdom of Saudi Arabia.
Methods: A pre-experimental group with pre-and post-test design was conducted from January 2022 to March 2022. Totally 200 Participants were selected using a non-probability purposive sampling technique. The questionnaire was prepared using the Global Youth Tobacco Survey (GYTS) and World Health Organization guidelines.
Results: Of the 199 participants, 91% had a family history of smoking, and 78.4% started smoking between the ages of 22–23. Most participants (48.2%) smoked 2–5 cigarettes per day. The mean knowledge score increased in the pretest from 4.49 (1.787) to post-test 17.5 (1.07), with a mean difference of 6.237. In perception, the maximum score was 55, while the mean score significantly increased from 21.65 (3.21) to 49.78 (2.69) in the post-test. Therefore, the educational intervention successfully improved both knowledge and perceptions of smoking.
Conclusion: Most participants had a family history of smoking and started smoking at a young age. This study highlights the need for increased awareness and education regarding the harmful effects of smoking and the development of strategies for smoking cessation.
Keywords: Awareness; Perception; Behavior; Smoking; Tobacco Control
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1901
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/102
2015-05-25T01:34:01Z
ALS:ART
nmb a2200000Iu 4500
"150525 2015 eng "
2310-5380
dc
Status of oxidative stress in breast cancer patients in Pakistani population
Javed, Sara
The University of Lahore, Lahore, Pakistan
Ali, Muhammad
The University of Lahore, Lahore, Pakistan
Ali, Fatima
The University of Lahore, Lahore, Pakistan
Anwar, Sanam Saiqa
The University of Lahore, Lahore, Pakistan
Wajid, Nadia
The University of Lahore
Molecular Biology; Biochemistry; Biotechnology
Background: Breast cancer is a major risk factor to the health of women around the globe and every year more than one million women are diagnosed with it. Pakistan is considered on the highest risk of breast cancer among Asian countries. One of the major reasons associated with higher incidence rate is oxidative stress condition in Pakistani women. The aim of this study was to analyze oxidative stress in patients with breast cancer in local population.
Methods: Activity of antioxidant enzymes i.e. superoxide dismutase (SOD) and catalase, level of reduced glutathione (GSH) were measured in the blood samples of breast cancer patients. To emphasize the results and the extent of lipid peroxidation in terms of malonyl dialdehyde (MDA) level were also evaluated. All the activities from cancer patients were compared with those of healthy people taken as control.
Results: The levels of GSH and MDA were found higher in the patients as compared to control group. While, activity of SOD and catalase enzymes was low in the diseased group.
Conclusion: Our study shows a significant oxidative stress in breast cancer patients in local population.
The RunningLine
2015-05-25 01:34:02
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/102
Advancements in Life Sciences; Vol 2, No 3 (2015): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/1061
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
DNA Fingerprinting and Cultivar Identification of Olive (Olea europaea L.) using SSR markers
Iqbal, Muhammad Zaffar
Agricultural Biotechnology Research Institute, AARI Faisalabad
Jamil, Shakra
Agricultural Biotechnology Research Institute, AARI Faisalabad
Shahzad, Rahil
Agricultural Biotechnology Research Institute, AARI Faisalabad
Ur Rahman, Sajid
Molecular Biology; Genomics
Background: Genetic diversity and population structure of the olive varieties cultivated in Pakistan are yet to be explored.
Methods: In present study, we studied population structure and genetic diversity and developed DNA fingerprints of 13 olive varieties using 63 Simple Sequence Repeat markers.
Results: Collectively 618 alleles were amplified among which 582 were polymorphic and 36 were monomorphic. High allelic diversity per locus was found among 63 SSR markers, i.e., one for GAPU-12 to 23 for UDO099-008 and GAPU-47 with an average 9.80 alleles per locus. On the basis of cluster analysis genotypes were grouped into two clusters. Cluster I contained varieties Manzanilla, Sohawa-selection, Koroneki, Bulkasar-selection, Arbequina, Arbosona, Chugtai-selection, whereas cluster II was comprised of varieties Bari-Zatoon, Coratina, Gemlik, Frontaio, Pendolino and Ottobratica. UDO-24 marker alone identified seven olive varieties. Similarly, DCA-07 and EMO-02 identified six olive varieties each.
Conclusion: The findings of this manuscript will be helpful for future studies related to DNA fingerprinting and genetic diversity assessment for choice of SSR markers and identification of olive varieties.
Keywords: Cluster analysis; Polymorphic Information Content; Structure analysis; Similarity matrix
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1061
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1758
2023-04-14T10:29:04Z
ALS:ART
nmb a2200000Iu 4500
"230414 2023 eng "
2310-5380
dc
Effectiveness of the α-tocopherol, ursodeoxycholic acid, and AZHEPOFIT phyto-complex against the statin model of drug-induced hepatitis
Jafarova, Rena
Azerbaijan Medical University
Abasova, Sevinj
Azerbaijan Medical University
Background: Drug-induced hepatitis is a common and serious side effect of many medications. Treatment for this condition is often difficult and limited, and there is a need for effective treatment options. The aim of this study was to investigate the effectiveness of α-tocopherol acetate, ursodeoxycholic acid, and AZHEPOFIT phyto-complex against the background of a statin model of drug-induced hepatitis.
Methods: The study was conducted on 70 white mongrel rats, divided into two groups and treated for 2 weeks twice a day. Statistical analysis was carried out by the Mann-Whitney-Wilcoxon method using MS EXCEL and S-PLUS software.
Results: Against the background of the statin hepatitis model, the combined use of the phyto-complex and ursodeoxycholic acid showed the best results. The atherogenicity index decreased from 16.03 to 2.29, the De Ritis ratio decreased from up to 1.49±0.05, and the severity of lipid peroxidation, the content of c-reactive protein, and medium molecular weight peptides decreased. All of these results indicate the restoration of the functional state of the liver.
Conclusion: The use of α-tocopherol acetate, ursodeoxycholic acid, and AZHEPOFIT phyto-complex can be an effective treatment option for drug-induced hepatitis caused by statin medication. The combination of phyto-complex and ursodeoxycholic acid showed the best results in reducing.
Keywords: Statin drug-induced hepatitis; α-tocopherol acetate; Ursodeoxycholic acid; AZHEPOFIT phyto-complex; Atherogenicity index; De Ritis ratio
The RunningLine
2023-04-14 10:29:04
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1758
Advancements in Life Sciences; Vol 10, No 1 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/339
2017-11-25T20:04:53Z
ALS:ART
nmb a2200000Iu 4500
"171125 2017 eng "
2310-5380
dc
Optimization of sulphuric acid pre-treatment of Acacia saw dust through box-bhenken design for cellulase production by B. Subtilis
Anjum, Aasiya
Department of Zoology, University of the Punjab new campus Lahore Pakistan
Irfan, Muhammad
Department of Zoology, University of the Punjab new campus lahore, Pakistan
Tabbsum, Fouzia
Department of Zoology, University of the Punjab new campus Lahore Pakistan
Shakir, Hafiz Abdullah
Department of Zoology, University of the Punjab new campus Lahore Pakistan
Qazi, Javed Iqbal
Department of Zoology, University of the Punjab new campus Lahore Pakistan
Background: Cellulases are enzymes which are capable of degrading lignocellulosic biomass. The current study is centred on optimization of dilute sulphuric acid pre-treatment of Acacia saw dust for maximizing cellulase production (CMCase and FPase). Hydrolysis or saccharification of lignocellulosic biomass is brought about by cellulases and the sugar thus released can be used for further bioethanol production.
Methods: Box- Bhenken design (BBD) was employed for optimization of pre-treatment conditions for Acacia saw dust. Three variables i.e. sulphuric acid concentration (0.6%, 0.8% and 1.0% v/v), substrate concentration (5%,10% and15%) and reaction time (4h,6h and 8h) was optimized. The pre-treated saw dust was used in the study as a substrate for producing cellulase enzyme through submerged fermentation by Bacillus subtilis (K-18).
Results: An optimum conditions i.e. (0.8% H2SO4 conc., 15% substrate conc. and 4h of reaction time) yielded highest filter paper activity (1.3617 IU/ml/min) and CMCase activity (0.7783 IU/ml/min). The suggested model was significant as revealed by F-value, coefficient of determination (R2) andP-value.
Conclusion: Results concluded that pre-treated substrate (Acacia sawdust) significantly increased cellulase production as compared to untreated substrate that could be utilized for further biofuel production.
The RunningLine
2017-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/339
Advancements in Life Sciences; Vol 5, No 1 (2017): Advancements in Life Sciences
eng
Copyright (c) 2017 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1015
2022-05-29T08:45:07Z
ALS:ART
nmb a2200000Iu 4500
"220529 2022 eng "
2310-5380
dc
Indigenous Curd as a Functional Food: A Source of Potential Pathogenic Bacterial Control
Yaqub, Shazia
Institute of Food Science and Nutrition, University of Sargodha, Sargodha-Pakistan
Murtaza, Mian Anjum
1Institute of Food Science and Nutrition, University of Sargodha, Sargodha-Pakistan
Ali, Shinawar Waseem
Institute of Agriculture Sciences, University of the Punjab, Lahore-Pakistan
Mushtaq, Sehrish
Institute of Agriculture Sciences, University of the Punjab, Lahore-Pakistan
Afzaal, Sohaib
Institute of Agriculture Sciences, Universityof the Punjab, Lahore
Farooq, Abdul Munim
Centre of Excellence for Molecular biology
Mustafa, Ghulam
Institute of Allied Health sciences, Lahore.
Molecular Biology;Microbiology
Background: Dahi is a functional food that is largely consumed in our community. The current study was designed to explore the efficacy of antibacterial agents that are present in the local indigenous curd of Lahore, Pakistan.
Method: Dahi was collected from various areas in Lahore. Bacterial cultures were isolated from this local curd and identified using a molecular technique that used 16S rRNA gene primers in PCR and sequencing. The antibacterial assay was carried out using the agar well diffusion method. Out of all the characterized bacteria isolate P26 displayed the best results for the control of pathogenic bacteria. Bacteriocin from isolate P26 was partially purified and its antibacterial assay was performed against sixteen indicator organisms and kanamycin was used as a standard for this experiment. Characterization of bacteriocin was performed at various temperatures, pH, and treating with enzymes.
Result: After the molecular characterization through 16S rRNA, Strain P26 was characterized as Bacillus vallismortis. A protease-sensitive antimicrobial substance was produced by Bacterial strain P26 (Bacillus vallismortis), isolated from local curd (Dahi). This isolate has a broad-spectrum inhibitory activity that can inhibit the growth of both food spoilage and pathogenic bacteria. According to results It was clear that protease-sensitive antimicrobial substance produced from strain P26 is more effective as compared to the antibiotic kanamycin. Maximum activity was shown against Staphylococcus species, a pathogen that is a common cause of gastroenteritis worldwide. This substance was active at 100 ºC for 30 minutes and pH (2-9). The activity was lost after treatment with proteases, so it is classified as bacteriocin.
Conclusion: Bacteriocin P26 is a more effective antibacterial agent than kanamycin, and it can be produced cheaply using local cardkey.
Keywords: Protease; Bacteriocin; Staphylococcus carnosus; Kanamycin
The RunningLine
2022-05-29 08:45:07
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1015
Advancements in Life Sciences; Vol 9, No 1 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1842
2023-11-26T05:22:36Z
ALS:ART
nmb a2200000Iu 4500
"230930 2023 eng "
2310-5380
dc
Neurokinin 1 receptor antagonist along with dexamethasone reduces the inflammation in COVID-19 patients: a novel therapeutic approach
Mehboob, Riffat
Lahore Medical Research Center LLP, Lahore, Pakistan
Ahmad, Fridon Jawad
2-
Department of Physiology, University of Health Sciences, Lahore, Pakistan
Pakistan
Qayyum, Ahad
3- Ahad Qayyum
Bahria International Hospital, Lahore, Pakistan
Pakistan
Rana, Muhammad Asim
4-
Bahria International Hospital, Lahore, Pakistan
Pakistan
Tariq, Muhammad Akram
5-
Higher Education Department (HED), Punjab, Lahore/ University of Health Sciences, Lahore, Pakistan
Pakistan
Tamur, Shadi
6-
Department of Pediatrics, College of Medicine, Taif University, Taif, Saudi Arabia
Saudi Arabia
Shahid, Imran
7-
Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Al-Abidiyah, P.O.Box 13578, Makkah, 21955, Saudi Arabia
Mirza, Iman M
8-
Public Health and Community Medicine, Family and Community Medicine Department, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia
Fathaddin, Amany
9-
Department of Pathology, College of Medicine, King Saud University and King Saud University Medical City, Riyadh, Saudi Arabia
Abusikkuen, Samy A.
10-
Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Rabigh, Saudi Arabia/Department of Anatomy, Ain Shams University, Cairo, Egypt
Saudi Arabia, Egypt
Zayed, Mohamed A
11- Mohamed A Zayed
Department of Physiology, Faculty of Medicine, King Abdulaziz University, Rabigh, Saudi Arabia/ Department of Medical Physiology, Faculty of Medicine, Menoufia University, Egypt
Saudi Arabia, Egypt
Aprepitant; COVID-19 infection; Treatment; Dexamethasone; Neurokinin-1 receptor; Substance P; Trigeminal nerve
Background: Corona virus infection is a respiratory infection, compromising the normal breathing in critical patients by damaging the lungs. The aim of this study was to evaluate the clinical outcomes of Substance P receptor Neurokinin 1 antagonist in COVID-19 patients against the usual treatments as controls.
Methods: It is a two-arm, open-label, randomized clinical trial that was carried out at Bahria International Hospital in Lahore, Pakistan. PCR-positive, hospitalized patients older than 18 years old, all sexes, and in the critical to life-threatening stage were included. 52 patients were placed in control group A and 67 patients were placed in intervention group B out of a total 119 patients who were randomly assigned to both arms. Before and after the intervention, lab tests were conducted in both groups. Aprepitant, a neurokinin-1 receptor antagonist, was additionally administered to the other arm while the other arm got standard therapy and care. Additionally, both groups received oral administration of the corticosteroid dexamethasone.
Results: Patients in group A were on average 56.05 years old, compared to 58.1 years old in group B. There were 24 women in group A and 28 in group B, while there were 28 men and 39 women in group A. Group A had three critical cases, but group B had six. The reduction in C-reactive protein in the intervention group, improvement in platelet count in group B, and normalization of ferritin and LDH levels in group B all indicated decreased inflammation in the biochemical and haematological parameters in both groups. However, because of the reduced sample size, it wasn't very significant.
Conclusion: The results of this recent trial provide a solid indication of Aprepitant's medicinal potential. Patients who got a combined therapy of dexamethasone and aprepitant had better clinical results, more favourable lab results, and lower levels of C-reactive protein, an inflammatory marker.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1842
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/697
2019-08-25T00:54:00Z
ALS:ART
nmb a2200000Iu 4500
"190825 2019 eng "
2310-5380
dc
Therapeutic potential of stem cells derived factor-1 alpha (SDF-1α) for skin burn injuries
Wajid, Nadia
The University of Lahore
Ali, Azib
Ali, Fatima
The University of Lahore
Latief, Noreen
University of the Punjab
Qazi, Aamer
The University of Lahore
Molecular Biology; Biotechnology
Background: Stem cells derived factor-1 alpha (SDF-1α) enhances the migration of bone marrow cells towards wound hence improve the healing process. The present study focuses on the therapeutic potential of SDF-1α and bone marrow derived cells from rats after SDF-1α treatment for skin burn.
Methods: SD rats were given thermal skin burn. Animals were divided into three groups i.e. SDF (S) with subcutaneous SDF-1α treatment after burn, Burn (B) and control (C). After seven days of treatment rats were sacrificed and skin, liver, heart, kidneys and blood were taken for histological and serological analysis. CBC, serum electrolytes, liver functions tests, renal functions tests, lipid profile, blood glucose as well as ELISA for VEGF, CRP and oxidative stress were performed. Cells were extracted from bone marrow and were transplanted in the burnt animals either directly or after culturing (Bone marrow stromal cells; BMSCs).
Results: A prominent role of SDF-1α on skin thermal wound healing was observed. Importantly the treatment has no significant impact on vital organs like liver, heart and kidney as well as physiological parameters. Lowered inflammation and enhanced angiogenesis was observed by ELISA for CRP and VEGF. BMSCs from SDF group showed higher growth (viability and proliferation), lowered cell death (LDH release), high angiogenesis (VEGF) and decreased oxidative stress. These BMSCs healed the burnt skin better than whole bone marrow of the same group after transplantation.
Conclusion: It may be concluded that SDF-1α can be a potential therapeutic agent for thermal skin burns and BMSCs after the treatment get higher growth and healing potential.
Keywords: Skin; Burn; CXCR-4; SDF-1α; BMSCs
The RunningLine
2019-08-25 00:54:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/697
Advancements in Life Sciences; Vol 6, No 4 (2019): Advancements in Life Sciences
eng
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1477
2022-11-05T03:24:51Z
ALS:ART
nmb a2200000Iu 4500
"221104 2022 eng "
2310-5380
dc
Frequency of Helicobacter Pylori Infections and Its Associated Risk Factors in Patients Attending Tertiary Care Hospital of Bhakkar, Pakistan
Nadeem, Haider
University Institute of Medical Laboratory Technology, The University of Lahore
Nawaz, Aiman
University Institute of Medical Laboratory Technology, The University of Lahore
Nawaz, Arooj
University Institute of Medical Laboratory Technology, The University of Lahore
Shams, Komal
University Institute of Medical Laboratory Technology, The University of Lahore
Mubarak, Bushra
University Institute of Medical Laboratory Technology, The University of Lahore http://orcid.org/0000-0001-9713-0028
Health studies, Microbiology
Background: Helicobacter Pylori is a gram-negative bacteria that is the main cause of chronic gastritis and plays a significant role in peptic ulcers, gastric carcinoma, and gastric lymphoma. The prevalence of H. pylori cases is 75-90% worldwide. The objective of the study was to determine the frequency of H. pylori and its associated risk factors in the Bhakkar district.
Methods: A total of 102 participants with problems in the gastrointestinal tract were taken from June 2021 to May 2022. Stool antigen was performed to confirm H. pylori infection. A complete blood count (CBC) was also performed on the blood sample.
Results: The current study showed that a total of 102 samples were collected in this study. It was concluded that out of 102 participants, 63 (61.8%) were H. pylori positive and 39 (38.2%) were H. pylori-negative participants. Females were more infected with H. pylori 38 (60.3%) as compared to males 25 (39.7). The frequency of factors such as smoking (52% vs 51%), weekly consumption of junk food (52.4% vs 43.6%), fizzy drinks (33.3% vs 23.1%) and drinking of unfiltered water (54% vs 53%) was more in H. pylori-infected group compared with the uninfected group but difference was not statistically significant with odds ratio less than 1.
Conclusion: The current study concluded that female genders, ethnicities, and history of stomach infection are risk factors for H. pylori. Exposure to Smoking, unfiltered drinking water, fizzy drinks, and Junk food is more in the affected group than in the unaffected group.
The RunningLine
2022-11-04 09:11:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1477
Advancements in Life Sciences; Vol 9, No 3 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2135
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Moxifloxacin ameliorates clinical disease activity and histopathological changes of acetic acid-induced colitis model in rat possibly through its effect on proinflammatory mediators and oxidative stress
Dawood, Jaffar O.
AL- Nahrain University, Baghdad
Abu-Raghif, Ahmed
AL- Nahrain University, Baghdad
Background: Moxifloxacin (MFX), a fluoroquinolone antibiotic, was found in previous study to exert an anti-inflammatory effect including reduction of proinflammatory cytokine expression, the aim of this research was to investigate the potential anti-inflammatory effect of MFX on acetic acid (AA)-induced colitis model in rats.
Methods: About 40 males of Wistar rats were divided into four groups as following, the negative (healthy) control group, AA-colitis group, sulfasalazine (SLS)group, and MFX group. Colitis induction was done by intra-rectal injection of 2 ml AA (4% v/v). After two hours of induction, SLS (100 mg/kg/day) and MFX (8 mg/kg/day) were given by oral route for seven consecutive days.
Results: The administration of MFX substantially decreased the disease activity index (DAI), and histopathological alterations triggered by AA. Moreover, AA induced a significant rise in the production of the proinflammatory cytokine IL-1β, the adhesion molecules ICAM-1, and the oxidative stress (OS) biomarker myeloperoxidase (MPO) in colon homogenate. the MFX administration significantly reduced the production of proinflammatory cytokine IL-1 β, adhesion molecules ICAM-1, and the OS biomarker myeloperoxidase (MPO) in colon homogenate, which were elevated by AA. The influences of MFX seemed comparable to those of SLS, with no significant difference between them.
Conclusion: Consequently, MFX may possess a beneficial property in the treatment of ulcerative colitis (UC).
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2135
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/950
2020-06-01T03:39:43Z
ALS:ART
nmb a2200000Iu 4500
"200601 2020 eng "
2310-5380
dc
Antagonistic, biofilm-forming rhizospheric Pseudomonas spp. isolated from Hail province
Sulieman, Abdel Moneim
Department of Biology, Faculty of Science, University of Hail, Hail, Kingdom of Saudi Arabia https://orcid.org/0000-0002-9202-6624
Sulieman, Abdel Moneim E.
Department of Biology, College of Science, Hail, P.O. 2440, University of Ha’il City 2440 – Saudi Arabia.
Veettil, Vajid N.
Department of Biology, College of Science, Hail, P.O. 2440, University of Ha’il City 2440 – Saudi Arabia.
Snoussi, Mejdi
Department of Biology, College of Science, Hail, P.O. 2440, University of Ha’il City 2440 – Saudi Arabia.
Molecular Biology; Biochemistry; Biotechnology, Microbiology
Background: The objectives of this study were to characterize Pseudomonas rhizospheric strains, that have a biocontrol activity, in rhizosphere soil in Hail province and study their ability to form biofilm.
Methods: Rhizosphere soil samples were collected from rhizosphere of soil plantation areas, to be used for bacteria isolation. The identified bacteria were qualitatively tested for their ability to produce slime and subsequently develop biofilm.
Results: The cultural and biochemical identification techniques, including morphological, biochemical and molecular methods revealed that the antagonistic bacteria- from the distinctive rhizosphere soil samples belong to Pseudomonas genus in particular, P. aeruginosa (PF1a, pf2a, PF-8) and P. putida (PF-7). These identified isolates inhibited Aspergillus niger development with percentage of parasitic growth inhibition greater than (48.095 ± 2.182)% for P. aeruginosa (pf-8). In addition, these identified isolates were significantly shown to be able to produce exopolysaccharide and subsequently develop biofilm on polystyrene and glass surfaces.
Conclusion: Superior strains of these bio-control and plant growth promoting rhizobacteria will enable for better biological control of fungal and bacterial plant diseases and may reduce chemical pesticide usage. The indigenous strains isolated could potentially have a great impact on controlling plant diseases, in particular, those caused by microorganisms, and could be used as an alternative bio control agents instead of harmful chemical pesticides. Most of the tried microbes produced exopolysaccharides as well as formed biofilm on polystyrene and glass surfaces.
Keywords: Biological control, Rhizosphere; Biofilm; Pseudomonas; Antimicrobial
The RunningLine
2020-06-01 03:39:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/950
Advancements in Life Sciences; Vol 7, No 3 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1285
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Influence of In-ovo Administration of Electrolyte on Eggs of Broiler Breeder During Perinatal Period and its Impact on Subsequent Broiler Performance
Imtiaz, Khwaja Yasir
Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan
Mukhtar, Nasir
Department of Poultry Science,
Faculty of Veterinary and Animal Sciences,
Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan
Yousaf, Arfan
Department of Clinical Studies,
Faculty of Veterinary and Animal Sciences,
Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan
Iqbal, Javed
Department of Poultry Science,
University of Agriculture, Faisalabad (Sub-Campus Toba Tek Singh),
Pakistan,
Ahmad, Tanveer
Department of Livestock Production and Management,
Faculty of Veterinary and Animal Sciences,
Pir Mehr Ali Shah Arid Agriculture University Rawalpindi,
Rawalpindi, Pakistan
veterinary sciences
Background: The perinatal period (final some days of pre-hatch to initial some days of post-hatch) is mainly critical moment during the growth of hatchling because it is a evolutionary time during which the hatchling undertakes catabolic, anabolic and physiological transfers from the consumption of egg components to external diet. Though, with the present endeavor of viable hatcheries as well as in view of instance to shift and release of hatchling to poultry farms, the hatchlings are unavoidably displayed to delay rationing from 48 to72 hrs. Consequently, tardy dieting, hatchling undergo deprivation as well as allocate the restricted deposits of nutrition food substances for maintenance of temperature modulation as well as anabolism & catabolism that hampers growth performance. In-ovo injection of nutrient (like, electrolytes) during last period of incubation can be applied as an approach to overcome above mentioned constraints.
Methods: A total 240 broiler breeder (Ross-308) fertile eggs were set in incubator trays representing 60 eggs for each treatment. A 200-μL electrolyte mixture solution (comprising NaCl 3.5g, KCl1.5g, Na3C6H5O7 2.9g and dextrose 20g) with volumes of 100, 500 or 1000ml insertion treatment as well as non-injected control were incorporated during this trial. The mixtures of electrolytes solutions with different concentration is used in all treatments with dose of 200-μL into amniotic fluid of hatching eggs at day 18 in hatchery, and subsequent hatchability, blood profile and post-hatch performance were examined.
Results: The results showed that none of the injections exhibited substantial (p > 0.05) influences on hatch rate or body weight (BW) at hatch, 3d as well as10d post hatch. Likewise, plasma refractive index (PRI), plasma triglyceride as well as glucose contents at d 3 & 10 were not influenced (p > 0.05) through any insertion treatments. Body weight gain (BWG), feed consumption and feed conversion ratio (FCR) throughout period at 0–32 weeks were also not influenced (p > 0.05) by in-ovo administration of the upgraded electrolyte mixture.
Conclusion: This study envisaged that tested electrolyte mixture fluids were showed safe and sound for the incipient and hatchlings. It may be proposed that electrolyte mixtures possess ability intended for usage in blend with other electrolytes, nutrition food substances as well as encourages to the viable insertion of broiler hatching embryos for the advancement of incipient and initial post-hatch chick growth as well as advancement.
Keywords: Electrolyte; In-ovo; Hatchability; Growth performance
The RunningLine
2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1285
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/85
2014-11-25T00:23:25Z
ALS:ART
nmb a2200000Iu 4500
"141125 2014 eng "
2310-5380
dc
Genotypic response of chickpea (Cicer arietinum L.) for resistance against gram pod borer (Helicoverpa armigera)
Shabbir, Muhammad Zeeshan
Arshad, Muhammad
Hussain, Babar
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan
Nadeem, Imran
Entomological Research Institute, Ayyub Agricultural Research Institute, Faisalabad, Pakistan
Ali, Shahbaz
Department of Entomology, University of Agriculture, Faisalabad, Pakistan
Abbasi, Asim
Department of Entomology, University of Agriculture, Faisalabad, Pakistan
Ali, Qurban
UAF
Enotomology
Background: Chickpea is an important pulse crop of Pakistan. The pod borer, Helicoverpa armigera (Hubner), is the major pest in most of the chickpea growing areas of the country. A field trial was carried out at Entomological Research Area, Ayub Agriculture Research Institute (AARI), Faisalabad, during growing season of 2012-13 to evaluate the resistance of chickpea genotypes against gram pod borer (Helicoverpa armigera).
Methods: Experiments were conducted in randomized complete block design (RCBD) with four replications having plot size of 3 ft × 20 ft. Morphological characters of chickpea like pod trichome, pod wall thickness, pod length, pod breadth, pod area and number of pods per plant were measured. The pod borer larval population/pod infestation was recorded from fifteen randomly selected plants per plot after ten days interval.
Results: It was found that the genotypes which had higher trichomes length and density and pod wall thickness were more resistant against Helicoverpa infestation. Significant genetic variation for resistance against Helicoverpa armigera attack was found in chickpea and variety K-70005 showed resistant behavior followed by K-08004 and K-60062.
Conclusions: It was concluded from prescribed study that the most susceptible genotypes were K-70005, K-08004 and K-70008 for the attack of Helicoverpa armigera to reduce grain yield.
The RunningLine
2014-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/85
Advancements in Life Sciences; Vol 2, No 1 (2014): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/879
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
Comparative Productive Performance of two Broiler Strains in Open Housing System
Khalid, Naqash
Poultry Research Institute Jaba Mansehra
Ali, Malik Mohsin
Poultry Research Institute Jaba Mansehra
Ali, Zubair
Poultry Research Institute Jaba Mansehra
Amin, Yasir
Poultry Research Institute Jaba Mansehra
Ayaz, Muhammad
Poultry Research Institute Jaba Mansehra
Background: The present study was conducted to compare the growth performance and ultimately to calculate the profitability of the two locally available commercial strains of broiler (Ross 308 and Cobb 500).
Methods: For the purpose of study, 900 number of day-old chicks (DOC) of each strain were purchased from the local market. The birds were reared in conventional broiler house with the provision of standard managemental conditions throughout the experimental period. The parameters recorded on weekly basis were feed intake, body weight gain, feed conversion ratio (FCR) and mortality.
Results: Result shown that the total body weight of Cobb-500 and Ross-308 on 1st week was 207.40±14 gram and 196.00±16 gram respectively and these result represented significant difference of weight gain (P<0.05) on 1st week of experiment among both the strains. From 2nd week of experiment till the last week (5th week) the results shown the total body weight of Cob-500 and Ross-308 as 2180.4±38 gram and 2103.7±36 gram respectively which was non-significantly different (P>0.05) among the strains. Furthermore, significant difference of feed conversion ratio (FCR) was observed (P<0.05) among both the strains but from day 7th till the market age weekly FCR of Cob-500 was significantly higher (P<0.05) than Ross-308. Comparatively high mortality (4.8±0.4%) was noticed in Ross broiler strain than Cobb broiler strain (3.7±0.4%).
Conclusion: It was concluded from the current study that the Cobb-500 is performing better in conventional open housing system at high altitude than Ros-308.
Keywords: Broiler; Cobb-500; Ross-308; Conventional broiler houses; Mortality
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/879
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1597
2023-04-14T10:29:04Z
ALS:ART
nmb a2200000Iu 4500
"230414 2023 eng "
2310-5380
dc
Prognostically Significant Fusion Oncogenes and Gene Mutations in Pakistani AML Patients
Tayyab, Muhammad
Institute of Zoology, University of the Punjab Quaid-e-Azam Campus, Lahore. http://orcid.org/0000-0002-4287-7631
Tariq, Zahra
http://orcid.org/0000-0002-7966-1420
Qadeer, Muhammad Imran
Khan, Mahwish
Fayyaz, Tuba
Akhtar, Tanveer
Background: The onset and progression of leukemia is associated with many genetic abnormalities including gene mutations and production of fusion oncogenes. Molecular studies on fusion oncogenes and mutations in different populations have been done. However, not much research on correlation of the fusion oncogenes with acute myeloid leukemia have been done in Pakistan.
Methods: Genetic analysis of 105 AML patients was done to investigate AML1-ETOand CBFB-MYH11 fusion oncogenes and mutations in NPM1 and NRAS genes. The genomic DNA and cDNA were subjected to amplification, electrophoresis, and Sanger sequencing.
Results: The frequency of AML1-ETO was 26% in AML patients and 34.2% in AML-M2 patients. CBFB-MYH11 was present in 11.4% AML patients. A total of six mutations in 4 regions of NPM1 gene and 2 regions of NRAS gene were detected. 3’UTR of NPMI gene had three variants; g.1128C>T (57.1%), g.1185-/T insertion (80.95%), and g.1163A>T (57.14%) while c.867_871subGTGGA >CAAGTTTGC (2.86%) was present in exon 12. NRAS gene had two mutations c.12C>T (51.4%) and c.33A>T (11.43%) in exon 2. c.867_871subGTGGA >CAAGTTTGC , and g.1163A>T in NPM1 gene and c.33A>T in NRAS gene were the novel findings in this ethnic population.
Conclusion: This genetic analysis may help to modulate the treatment strategies and improve survival of patients.
Keywords: Acute Myeloid Leukemia; Fusion Oncogenes; AML; AML1-ETO; CBFB-MYH11; AML-M2; Nucleophosmin, NPM1; NRAS
The RunningLine
2023-04-14 10:29:04
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1597
Advancements in Life Sciences; Vol 10, No 1 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1945
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
hSSB1 (NABP2) as Prognostic for Worker's Exposure to Oxidative Stress
Kadhum, Rafed Abbas
Wasit University, College of Science, Department of Pathological Analysis. Kut, Iraq
Alqurashy, Nasser Nafaa Abrahem
Wasit University, College of Science, Department of Pathological Analysis. Kut, Iraq
Sabah, Jinan Tuma
Wasit University, College of Science, Department of Pathological Analysis. Kut, Iraq
Molecular Biology
Background: Oxidative stress can be defined as a phenomenon brought on by an imbalance between biological system's capacity for detoxifying such reactive products and the formation and build-up of ROS in tissues and cells. X-rays have an effect on the systems of the human body, hSSB1 (NABP2) plays a crucial role in DNA damage responses by taking part in a repair regarding double-strand DNA breaks and the base excision repair of oxidized guanine residues (8-oxoguanine). Here, we show that hSSB1 stabilizes as an oligomer in response to oxidative stress, which is necessary for the hSSB1 to remove 8oxoguanine.
Methods: In this study, the detection of NABP2 (SSB-I) (Single-stranded DNA binding protein (ATM / MRN path-way)) expression through QRT-PCR is considered a key method. Depending on the Centre for Radiation Protection, employees exposed to weak doses of y -rays approximately (2-10) cGy could experience X-ray Geno toxicity. The exposure group worked in close proximity to the source (X-ray) and control group. Whole blood had been collected from all of the individuals, according to the findings.
Results: NABP2 (hSSBI) expression increased in people who were exposed for longer than lcGy and decreased in the controls. was enhanced as a result of the Geno toxicity caused by low doses of X-rays. X-ray oxidative stress stimulates. Single-stranded DNA (ssDNA)-binding proteins like OBFC2B, which bind to single-stranded DNA (NABP2; SSBI).
Conclusion: In the present study, the conclusion that has been reached is that the exposure of the individuals to the low dosages from the I-DIR had led to the occurrence of some slight symptoms that can't be noticed via outside signs as in the high dosages, therefore, it we have studied the changes in gene expression like XRCC-I, XRCC-2, FOXM-I, it was researched hSSB1 (NABP2) for the purpose of knowing effects of the I-DIR. Using radioisotopes in industry, science, and medicine, must be implemented in such a way as to keep the environment and people safe for a long time.
Keywords: Oxidative stress; NABP2 (SSBI); Transcription factor; Biomarker; x-ray; Diagnosis; Reactive oxygen species (ROS)
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1945
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/269
2017-02-27T22:34:07Z
ALS:ART
nmb a2200000Iu 4500
"170225 2017 eng "
2310-5380
dc
Biosafety risk assessment approaches for insect-resistant genetically modified crops
Ullah, Inaam
Department of Biotechnology, Shaheed Benazir Bhutto University (SBBU), Sheringal, Pakistan
Asif, Muhammad
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
Ranjha, Mazhar Hussain
Department of Entomology, University of Agriculture, Faisalabad, Pakistan
Iftikhar, Romana
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
Department of Botany, Government College University for Women, Faisalabad, Pakistan
Ullah, Midrar
Department of Biotechnology, Shaheed Benazir Bhutto University (SBBU), Sheringal, Pakistan
Khan, Nasir Mehmood
Department of Biotechnology, Shaheed Benazir Bhutto University (SBBU), Sheringal, Pakistan
Ashfaq, Muhammad
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada
Biotechnology; Biosafety; Toxicology
Background: Environmental risk assessment (ERA) is imperative for commercial release of insect resistant, genetically modified crops (IR-GMCs).An insect specific, spider venom peptideω-HXTX-Hv1a (Hvt) was successfully expressed in cotton plants. The cotton plants producing Hvt protein have demonstrated resistance against economically important insect pest species. The study was performed to assess the effects of Hvt producing cotton plants on Honey bees (Apis mellifera).
Methods: Three approaches were used to evaluate the effects of Hvt protein on adults of honeybees; whole plant assays in flight cages, in vitro assays with pollen of Hvt-cotton, and assays with elevated levels of purified Hvt protein. Pollens of Bt cotton or purified Bt proteins were used as control.
Results: The field experiments did not yield any meaningful data due to high rate of mortality in all treatments including the control. However, the laboratory experiments provided conclusive results in which Hvt, purified or in pollens, did not affect the survival or longevity of the bees compared to the control. During the course of study we were able to compare the quality, effectiveness and economics of different experiments.
Conclusions: We conclude that Hvt either purified or produced in cotton plants do not affect the survival or longevity of honey bees. We are also of the view that starting at laboratory level assays not only gives meaningful data but also saves a lot of time and money that can be spent on other important questions regarding safety of a particular transgenic crop. Hence, a purpose-based, tiered approach could be the best choice for pre-release ERA of IR-GMCs.
The RunningLine
2017-02-25 06:24:28
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/269
Advancements in Life Sciences; Vol 4, No 2 (2017): Advancements in Life Sciences
eng
Copyright (c) 2017 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1180
2022-07-05T10:53:57Z
ALS:ART
nmb a2200000Iu 4500
"211231 2021 eng "
2310-5380
dc
Socio-economic effects of COVID-19 – a study of the University students and teachers in Pakistan
Ullah, Inam
The University of Lahore
Murtaza, Bibi Nazia
The University of Haripur 22620 Pakistan
Mumtaz, Muhammad
The University of Haripur 22620 Pakistan
Tabassum, Sadia
Hazara University, Garden Campus, Mansehra 21300 Pakistan
Khan, Aziz ud Din
Department of Genetics, Hazara University, Garden Campus, Mansehra 21300 Pakistan
Mushtaq, Rubina
The University of Lahore
Qasim, Muhammad
Govt College University Faisalabad, Pakistan
Khan, Muhammad Fiaz
Hazara University, Garden Campus, Mansehra
Malik, Arif
The University of Lahore
Health and Epidemiological studies
Background: Coronavirus Disease-2019 (COVID–19) has appeared as a deadly pandemic affecting most of the countries across the world. The disease has caught humanity unprepared; therefore, there has been a lack of awareness about the causes, and transmission and mortality rates. It has also affected the overall socioeconomics from individual to worldwide levels. COVID-19 is an unprecedented and first-time challenge for Pakistan and a lockdown imposed by the Government of Pakistan has further devastated the economy of the country. This online survey was conducted to know the effects of the Covid-19 pandemic on the social life and economic status of the people of Pakistan. In this article, we mainly focused on teachers and students from different Universities of Pakistan for data generation because they can provide better information due to the use of modern technologies as a source of information.
Methods: An online questionnaire survey covering 11 parameters including age, gender, employment status, mortality rate, education, marital status and, information about the pathogen responsible for the disease was filled by 1260 individuals from Pakistan.
Results: The results obtained show that 56.6% of the responders were males,43.4% were females, 59.9% were unmarried, 37.7% were graduate students and, 48.7% unemployed. Most of the responders (55.6%) said that COVID-19 negatively affected the jobs of the people. We found (93%) of the responders with good knowledge about COVID-19.
Conclusion: We conclude from the results that most of the responders were males, educated and they knew the pathogenic effect of the COVID-19 virus on humans. These results also show that the disease has drastically affected the economy and social life of the people of Pakistan.
Keywords: Coronavirus; COVID-19; Knowledge; Socio-economic; Pakistan
The RunningLine
2022-02-06 09:46:24
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1180
Advancements in Life Sciences; Vol 8, No 4 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1446
2023-11-02T15:47:42Z
ALS:ART
nmb a2200000Iu 4500
"230930 2023 eng "
2310-5380
dc
Chemo-Modulatory Potential of Flaxseed Oil as Natural Anticancer Therapeutic
Batool, Aliza
Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture Multan (Pakistan)
Farooq, Umar
Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture Multan (Pakistan)
Shafi, Afshan
Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture Multan (Pakistan)
Khan, Zulqurnain
Muhammad Nawaz Shareef University of Agriculture Multan Punjab Pakistan
Ikram, Rao Muhammad
Department of Agronomy Muhammad Nawaz Shareef University of Agriculture Multan Punjab Pakistan
Shahbaz, Muhammad
Department of Food Science Muhammad Nawaz Shareef University of Agriculture Multan Punjab Pakistan
Firdous, Nida
Department of Food Science & Technology, MNS-University of Agriculture, Multan - Pakistan
Iqbal, Mariam
Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture Multan (Pakistan)
Abbas, Naqi
Department of Food Science & Technology, MNS-University of Agriculture, Multan -
Pakistan
Rafiq, Zahid
Department of Food Science & Technology, MNS-University of Agriculture, Multan -
Pakistan
Health and Epidemiological studies
Background: Cancer is a disease which is characterised by uncontrolled cell proliferation and development. Surgery, chemotherapy, radiotherapy, and photodynamic therapy are the most frequent cancer treatments. On the other hand, there are many negative health impacts of radiation and chemotherapy that limit the efficient use of these therapies.
Methods: This scenario needs natural treatments that are cost-effective and has no adverse effects. Flaxseed oil can be used as a nutraceuticals for the management of cancer. The current research was aimed on the exploration of the flaxseed (Linum usitatissimum) oil for in vitro anticancer activity as a natural therapy for the management of cancer.
Result: The flaxseed powder contained 36.6±0.04% oil contents with an average yield of 36.6±0.03% by using hexane as solvent for extraction. Moreover, the oil contained polyunsaturated fatty acid with omega 3 fatty acid (alpha-linolenic acid) as a dominant content of the oil. In-vitro anticancer activity of flaxseed oil was observed by Cytotoxic (3T3 cell line) and Prostate Cancer (PC3 cell line) indicating that the oil possessed anticancer activity which was dose-dependent.
Conclusion: On the basis of results, it was concluded that the flaxseed or its oil can be used for the management of cancer as a natural therapy by using optimized dose levels for a different types of cancer.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1446
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/660
2018-11-25T08:03:45Z
ALS:ART
nmb a2200000Iu 4500
"181125 2018 eng "
2310-5380
dc
Molecular typing of Methicillin Resistance Staphylococcus aureus(MRSA) isolated from device related infections by SCCmec and PCR-RFLP of coagulase gene
Sohail, Muhamad
Department of Microbiology and Molecular Genetics, University of the Punjab Lahore, Pakistan http://orcid.org/0000-0001-5035-5019
Latif, Zakia
Department of Microbiology and Molecular Genetics, University of the Punjab Lahore, Pakistan
Microbiology; Molecular Biology;
Background: SCCmec and PCR-RFLP are productive and cost-effective methods for epidemiological investigations and source tracking of MRSA. Aim of this study was to investigate the epidemiology and molecular characterization of MRSA recovered from device related infection. Methods: A total of 626 MRSA were collected from prosthetic device related infections and subjected for presence of mec gene and SCCmec typing. SCCmec characterized MRSA were subjected for agr typing followed by RFLP genotyping and amplification of PVL gene. Following the Clinical & Laboratory Standards Institute (CLSI), antibiotic resistance pattern was determined. Results: Of 626 MRSA isolates, 488(78%) were characterized by SCCmec typing. Most common type was SCCmec IV (43%), followed by SCCmec II (25%) SCCmec III (22%) and only 10% was SCCmec V. SCCmec characterized MRSA strains were 100% resistance to tobramycin and chloramphenicol, 96% resistance to ciprofloxacin and 93% resistance to azithromycin. The genePVL was only present in SCCmec IV. All agr typed MRSA strains were resistance to gentamicin, tobramycin and chloramphenicol. PVL was present in all strains of agr III, 80% of agrIV, 50% of agrII and absent in agrI. RFLP analysis resulted in 16 types of non-duplicate unique bands pattern which were equally distributed among prosthetic device related infection. Conclusion: PVL harboring SCCmec or agr typed MRSA strains are less resistance to antibiotics. RFLP is simple, productive and cost-effective method for molecular typing of MRSA. Correct use of antibiotics and molecular surveillance is indispensable to detect the change in epidemiological and antibiotic resistance trends of MRSA.
The RunningLine
2018-11-25 08:03:45
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/660
Advancements in Life Sciences; Vol 6, No 1 (2018): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/660/2260
https://submission.als-journal.com/index.php/ALS/article/download/660/2261
https://submission.als-journal.com/index.php/ALS/article/download/660/2262
Copyright (c) 2018 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1373
2022-08-27T03:36:01Z
ALS:ART
nmb a2200000Iu 4500
"220827 2022 eng "
2310-5380
dc
The Association of Nine Novel Variants in the ZEB2 Gene with Serum Levels of TNF-α, TGF-β and IFN-γ
Rabae, Zahraa Hashim
University of Kerbala
Gaaib, Jawdat Noori
University of Kerbala
Background: ZEB1 and ZEB2, are expressed by a variety of immune cells of both myeloid and lymphoid lineages, they act to regulate important transcriptional networks necessary for cell differentiation, maintenance, and function. The study was carried out in the Department of Clinical Laboratories / College of Applied Medical Sciences / University of Kerbala during the period from January 2021 to September 2021. The study aimed to shed light on the association between the variation of the ZEB2 gene and serum levels of some cytokines (TNF-α, TGF-β, INF-γ) involved in immune cell differentiation in the Iraqi population.
Methods: A total of 80 healthy volunteers, randomly chosen, were enrolled in this study, with an age ranged 20-50 years at the time of the investigation. The variation of the ZEB2 gene was investigated by the Sanger sequencing method. The serum levels of TNF-α, TGF-β and INF-γ were measured by ELISA.
Results: The results identified presence of nine novel non-registered variants in 27 samples, 5 out of 9 variants were detected in exon1 region, while the other 4 were detected in the 5`UTR region. These variants might be of interest to the ZEB2 gene studies due to their presence in the coding and regulatory regions that regulate the gene expression.
Conclusion: The two variants, 144520053 G/A and 144520162 T/A, among the nine novel variants might be the most important variants because both showed significant effects on some study parameters.
Keywords: Novel variants; ZEB2; TNF-alpha; TGF- beta; IFN-gamma
The RunningLine
2022-08-27 03:36:01
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1373
Advancements in Life Sciences; Vol 9, No 2 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2020
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Correlation of Osteocalcin and Fetuin-A levels with Renal Function and Other Biomarkers in CKD patients from Baghdad
Hussein, Wissam Jabbar
Medical Laboratory Science Technologist, Medical City, Baghdad Teaching Hospital, Baghdad
Hassan, Hiba Abid Al-Hussein
College of Health and Medical Technologies, Middle Technical University, Baghdad
Gata, Abbas Muhsin
College of Health and Medical Technologies, Middle Technical University, Baghdad
Background: This investigation aims to determine if a correlation existed between the levels of Osteocalcin, Fetuin-A, and other biochemical markers in patients with CKD compared to healthy.
Methods: One hundred eighty samples divided into 120 (CKD) patients in end-stage divided into 70 males and 50 females, and 60 healthy controls 35 males and 25 females were taken between November 2022 and March 2023 from three major hospitals in Baghdad. diagnosed using symptoms, biochemical testing, history, and clinical examination by specialized nephrologists. Urea, creatinine, calcium, albumin, and phosphate were measured by spectrophotometer (Roche Cobas C111). Furthermore, a spectrophotometer (Beckman coulter au480) was used to measure sodium and potassium levels. On the other hand, vitamin D3 level and PTH were measured by spectrophotometer (Roche Cobas E411). Moreover, Osteocalcin and Fetuin-A were done by ELISA technique.
Results: The present study found that the male patients are more than females, and the highest percentage of CKD cases were found in the age group of 50-59 years. also, indicates a strong positive correlation between the levels of Fetuin-A and GFR. while a strong negative correlation between the levels of Fetuin-A with creatinine, urea, and Osteocalcin, moreover, a strong negative correlation between the level of Osteocalcin with GFR and Fetuin-A. The study also found a strong positive correlation between the levels of Osteocalcin with urea and creatinine.
Conclusion: Decreased Fetuin-A concentrations have been linked to increased calcification in the vasculature and mortality, increased Osteocalcin levels in CKD may not confer a protective effect against bone disorders.
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2020
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/705
2020-06-01T03:39:43Z
ALS:ART
nmb a2200000Iu 4500
"200601 2020 eng "
2310-5380
dc
Risk factors associated with relapse of drug dependence after treatment and rehabilitation in areas under the influence of war on terror
Abdullah, Muhammad
Department of Pharmacy, Kohat University of Science and Technology, 26000 Kohat, KPK, Pakistan
Khan, Muhammad Imran
1. Department of Pharmacy, Kohat University of Science and Technology, Kohat 26000, KPK, Pakistan.
2. Drug Detoxification & Health Welfare Research Center, Bannu 28100 KPK, Pakistan. http://orcid.org/0000-0003-4777-5084 http://orcid.org/0000-0003-4777-5084
Mumtaz, Faiza
Experimental Medicine Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran
Shah, Faridullah
Drug Detoxification and Health Welfare Research Center, Bannu 28100, KPK, Pakistan
Ximenes, Rosana C
Universidade Federal de Pernambuco, Campus da UFPE – CCB/CCS, CEP: 50901-970, Recife, Brazil.
Nikoui, Vahid
Razi Institute for Drug Research, Iran University of Medical Sciences, Tehran, Iran
Wahab, Abdul
Department of Pharmacy, Kohat University of Science and Technology, Kohat 26000, KPK, Pakistan
Background: People exposed to war on terror are more prone to neuropsychiatric disorders and drug addiction. The present study was aimed to investigate the risk factors associated with relapse of drug dependence after treatment and rehabilitation in areas under the influence of war and terrorism.
Methods: Total 57 individuals who had a relapse from the war affected area were included in current study. Retrospective data were collected from Drug Detoxification and Health Welfare Research Center, Bannu, KPK, Pakistan. Along with demographic characteristics, current study also focused on the prevalence of psychological problems due to war, in association with prevalence of relapse.
Results: A total of 93% of Individuals with relapse have concurrent stress. Similarly, depression was also common in 78.9% of relapsed individuals. Relapse was more common in Polydrug abusers (66.7%) as compared to single drug abusers. As far as abstinence duration is concerned, subjects having depression and stress due to war, relapsed in less time as compared to individuals with no psychological disorders.
Conclusion: Wars are related with mental and psychological problems like stress and depression and these factors significantly contribute to the relapse of drug dependence as suggested by results of current study.
Keywords: Drug addiction; Relapse; War on terror; Depression
The RunningLine
2020-06-01 03:39:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/705
Advancements in Life Sciences; Vol 7, No 3 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1509
2022-11-05T03:24:51Z
ALS:ART
nmb a2200000Iu 4500
"221031 2022 eng "
2310-5380
dc
A study on the Impacts of COVID-19 on health, Economy, Employment and Social Life of People in Indonesia
Rehman, Saifur
Airlannga University
Nnabuike, Ugbo Emmanuel
Ebonyi State University, Abakaliki, Nigeria
Abbas, Asghar
Muhammad Nawaz Sharif University of Agriculture Multan, Pakistan
Rahman, Attaur
The Chinese University of Hong Kong 999077 (CUHK), Hong Kong SAR
Malik, Uzma
East medical ward King Edward Medical University
Effendi, Mustofa Helmi
Univeristas Airlangga
Hussain, Kashif
Muhammad Nawaz Sharif University of Agriculture Multan, Pakistan
Raza, Muhammad Asif
Muhammad Nawaz Sharif University of Agriculture Multan, Pakistan
Health and Epidemiological studies
Background: The aftershocks of COVID-19 pandemic are still emanating in different regions of the world in term of increasing number of cases and deaths due to mutation in the virulence and pathogenicity of the virus. The pandemic affected almost every part of our lives including health, economy, employment, and social interactions. This study surveyed the Indonesian public to better understand their health, employment, and economic deterioration during the early stages of the COVID-19 outbreak.
Methods: An online cross-sectional survey of 200 participants was conducted from eight different regions (Jawa Timur, DKI Jakarta, Kalimantan Tengah, Yogyakarta, Bali, Sulawesi Selatan, Jawa Tengah) of Indonesia who speak Bahasa. A standardized questionnaire was used to obtain information about COVID-19 impacts on health, employment, the economy, and social life from the respondents. Descriptive statistics and Chi-square tests were conducted to analyze the data.
Results: According to the findings, out of 200 participants, 40% stated that the impact of COVID-19 did not affect their salary. People under the age of 20 with an intermediate education who worked in government sectors were more likely to lose their jobs (p-value 0.05), which would result in a loss in salary that would have an impact on the education of their children. Only the "use of hand sanitizers" indicated a statistically significant difference between the practices of male and female respondents (p-value = 0.038), which is one of the activities that helps to prevent fever and respiratory difficulties during the present pandemic.
Conclusion: The finding of the study depicted that COVID-19 has no immediate collateral effects on the economy of the study participants. However, the pandemic has a negative impact on the employment, health, and social life of the people. To mitigate the negative effects of this pandemic on health, employment, economy, and social life, a complete evaluation of COVID-19 impacts, as well as public health interventions, should be conducted.
The RunningLine
2022-11-04 09:11:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1509
Advancements in Life Sciences; Vol 9, No 3 (2022): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/1509/6897
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2100
2024-01-08T07:56:30Z
ALS:ART
nmb a2200000Iu 4500
"240108 2024 eng "
2310-5380
dc
Traceability environmental effects of microfabric in leaves of Cupressus dupreziana plant and Soil Surrounding it given the rise in COVID19
Ati, Estabraq Mohammed
Abdulmajeed, Awatif M.
Biology Department, Faculty of Science, University of Tabuk, Umluj 46429, Saudi Arabia
Alharbi, Basmah M.
Biology Department, Faculty of Science, University of Tabuk, Umluj 46429, Saudi Arabia
Ajmi, Reyam Naji
Department of Biology Science, Mustansiriyah University, POX 46079, Iraq-Baghdad.
Latif, Abdalkader Saeed
National University of Science and Technology / College of Health and Medical Technology
Background:The high death rate from COVID-19, which can increase mineral pollution in cemetery soils and necessitates future cemetery projects that are able to counteract these environmental effects, highlights the significance of studying metal concentrations in cemetery soils. The research analyzed the quantities of minerals in the cemetery soil in Najaf Governorate, Iraq, taking into account the high mortality during the sampling period in order to identify future projects for cemeteries targeted at mitigating environmental consequences.
Methods: Using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM), the researchers investigated the leaf features of the soils in three cemeteries. soil samples were taken three times from a depth of 10 to 30 cm Cupressus dupreziana plants considering the analysis in triplicate with averaging, analytical deviation and used in the autoregressive integrated moving averages model, this curve had 50% traceability to bioavailability to reduce contamination risk.
Results: The findings revealed elevated amounts of iron and lead (Pb) (Fe), tomb soil analysis. Take into account the rise in fatalities and the associated funerals. According to COVID-19, the estimated daily mortality toll is between (20-50); however, metal pollution may be more severe. In order to lessen the effects on the environment, the facility was expressed as a gas and effluent processor at 80% in soil of the proportion of the curve in the spatial analysis for mineral contamination.
Conclusion: The SEM and EDX can serve as reliable indicators, while the morphometric approach offers a holistic understanding of the soil and its associated plant life by analyzing elemental compositions. Employing these methods allows for quantitative assessment and comparison against the control, aiding in the thorough characterization of Cupressus dupreziana. Furthermore, these analyses shed light on the various organism clusters found within the study area's soil.
The RunningLine
2024-01-08 07:56:30
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2100
Advancements in Life Sciences; Vol 10, No 4 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/841
2020-12-01T03:48:08Z
ALS:ART
nmb a2200000Iu 4500
"201127 2020 eng "
2310-5380
dc
In vitro Micropropagation of Citrullus colocynthis (L.) Schrad: an endangered medicinal plant
Tariq, Arneeb
Govt. College Women University, Faisalabad.
Afrasiab, Humera
University of the Punjab, Lahore
Farhat, Fozia
Govt. College Women University, Faisalabad
Plant sciences; Plant Tissue Culture
Background: The experiment describes the establishment of in vitro conditions for seed germination, micropropagation, callogenesis, organogenesis and acclimatization of Citrullus colocynthis (L.) Schrad, of family Cucurbitaceae.
Methods: In vitro grown seedlings from decontaminated seeds were micropropagated in basal MS medium at 23±2oC temperature and light intensity of 3000 Lux for 16 hours in culture room. In vitro grown nodal explants were supplemented with BAP (6-Benzylaminopurine) and NAA (Naphthalene acetic acid) with basal MS (Murashige and Skoog) medium to induce multiple shoots. Indole butyric acid (IBA; 0.1 to 2.0 mg/L) was supplemented to MS medium to develop roots of micropropagated shoots. Internodes and leaves of micropropagated shoots used to induce callus in MS medium enriched with varying concentration of 2, 4- dichlorophenoxyacetic acid (2, 4 D; 0 to 2.0mg/L) and kinetin (KIN; 0 to 1.0mg/L). Shoot initiation from callus was tested by adding 2, 4-D (0.1 to 2.0 mg/L) and BAP (1.0 to 1.5 mg/L) in basal MS medium. Conditions were carefully monitored during the experiment. After hardening, the micropropagated plantlets were placed in open filed environment in pots filled with sand and peat moss (3:1).
Result: Surface sterilized seeds of Citrullus colocynthis (L.) showed 100% germination in regulator free medium. Significantly mature shoots (75%) from nodal explant recorded in BAP (2.0 mg/L) and NAA (1.0 mg/L) augmented MS medium. Highest number (90%) of roots per shoot explant were observed in IBA (2.0 mg/L). Leaf explants showed better response to form callus with a combination of 2, 4-D (1.0 mg/L) and KIN (1.0 mg/L) and further rise in 2, 4-D concentration caused a sharp decrease in callus formation. Shoot induction from callus cultures observed in MS medium containing 2, 4-D (2.0 mg/L) and BAP (1.5 mg/L), producing an average of 10 shoots per culture. Plants were effectively transplanted in open environment with survival rate of 85%.
Conclusion: Results indicate the successful establishment of the growth room conditions for in vitro micropropagation of the endangered medicinal plant, Citrullus colocynthis.
Keywords: In vitro; Indole butyric acid; callus induction; Citrullus colocynthis; organogenesis
The RunningLine
2020-11-27 03:56:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/841
Advancements in Life Sciences; Vol 8, No 1 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1135
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Ultrasound Detected Non-Alcoholic Fatty Liver Disease Prevalence and its Risk Factors in Pakistani Population
Waseem, Muzna
Department of Medical Imaging, School of Health Sciences, University of Management and Technology
Saeed, Fizza
Department of Medical Imaging, School of Health Sciences, University of Management and Technology
Khan, Rida
Department of Nutrition Science, School of Health Sciences, University of Management and Technology
Non-Alcoholic Fatty Liver Disease; NAFLD; Pakistani; Obesity; Cirrhosis
Background: Nonalcoholic fatty liver disease (NAFLD) is the most common predictor of chronic liver disease. It is an emerging health condition in Pakistan and in most cases, the disease remains undiagnosed due to a low trend of screening processes observed over the years. The purpose of this research was to assess the relationship of ultrasonography (USG) – detected NAFLD with the risk factors including obesity, diabetes, hypertension, and menopause.
Methods: This cross-sectional study evaluated the data gathered from medical histories, anthropometric measurements, and diagnostic abdominal ultrasound scans of 87 patients referred to radiology department of a trust hospital in Lahore, Pakistan. Patient diagnosed with NAFLD without any other disease or complication were selected through purposive sampling for further study.
Results: The results depicted a higher prevalence of NAFLD in females. Among diagnosed NAFLD patients, most (33.7%) showed signs of mild fatty liver on USG. Statistically, a significant (p value= 0.002) relationship of obesity with NAFLD was observed. NAFLD patients had higher mean BMI values (31.04 ± 4.67 Kg/m2, p value = 0.000) as compared to other patients (25.34 ± 4.71 Kg/m2), confirming that obesity is the most important risk factor for NAFLD. Other risk factors (diabetes, hypertension, and menopause) were not found to be significantly related to NAFLD.
Conclusion: The major finding of this study is that higher BMI increases the likelihood of developing NAFLD. Prevention and treatment of overweight and obesity can reduce the prevalence of NAFLD.
Keywords: Non-Alcoholic Fatty Liver Disease; Obesity; Cirrhosis
The RunningLine
2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1135
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2628
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
Ringworm detection using the instance of segmentation potential of YOLOv7 in dromedary camels
Alhashmi, Fawaghy
Fujairah research cenetre
Mansour, Nabil
1 Fujairah Research Centre (FRC), Al-Hilal Tower 3003, P.O. Box 666, Fujairah, United Arab Emirates.
2 Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.
Bano, Shaher
Fujairah Research Centre, United Arab Emirates https://www.frc.ae/
Lamghari, Fouad
Director Fujairah Research Centre
Vetrenery sciences, health and epidemiological studies
Background: Dermatophytosis, commonly known as ringworm, is a contagious fungal skin disease prevalent among camels, particularly of 1-3 years old, and resulting in considerable economic losses. Recently, using machine learning technology presents a promising avenue for achieving high diagnostic accuracy, especially in identifying infected camels in remote farm settings.
Methods: A dataset comprising 801 images from 61 camels aged 12-15 months was subjected to analysis using YOLOv7 (You Only Look Once, volume 7), a Machine learning model. Subsequently, the YOLOv7 algorithm's efficacy in distinguishing between healthy and ringworm-infected camels was evaluated.
Results: The YOLOv7 algorithm demonstrated robust capability in accurately identifying ringworm-infected skin and distinguishing it from healthy skin in camels. Validation set results revealed an average precision (AP) of 0.944, indicating high effectiveness in discriminating between normal and infected skin. Furthermore, the algorithm exhibited proficiency in classifying the severity of skin infection among the identified cases. Of the 61 camels analyzed, 36 were found to be infected, representing an incidence rate of 59%. These infected camels were further categorized into groups based on the severity of infection, with 6 classifieds as mild (<50 infection spots), 5 as moderate (50-100 spots), and 25 as severe (>100 spots).
Conclusion: The YOLOv7 model emerges as a dependable tool for the identification and classification of camel ringworm infections. Its implementation incorporated in farm surveillance and notification system holds promise for early detection of such issues and effective monitoring of camels kept under farm conditions in remote areas.
Keywords: Dromadory camels; Computer vision; YOLO7; Ringworm; Dermatophytosis, UAE
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2628
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/175
2016-11-24T01:24:55Z
ALS:ART
nmb a2200000Iu 4500
"160225 2016 eng "
2310-5380
dc
Screening for drought tolerance: comparison of maize hybrids under water deficit condition
Ali, Qurban
a. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
b. Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Ahsan, Muhammad
a. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Malook, Saif-ul-
a. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
e. Kunming Institute of Botany, University of Chinese Academy of Sciences, China
Kanwal, Naila
a. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Ali, Fawad
a. Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Ali, Arfan
c. Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Ahmed, Wazir
c. Department of Soil & Environmental Sciences, MNS
University of Agriculture Multan, Pakistan
Ishfaq, Muhammad
d. Molecular Genetics Research Laboratory, Department of Botany, University of the Punjab Lahore, Pakistan
Saleem, Muhammad
d. Molecular Genetics Research Laboratory, Department of Botany, University of the Punjab Lahore, Pakistan
Background: Maize is an important cereal crop, grown throughout the globe for human food and livestock feed, but biotic and abiotic factors had shown adverse effects on biomass and grain yield. Changing climatic conditions have imposed drought (water scarcity) as a major problem to combat with yield losses and biomass in maize and other cereals.
Methods: The prescribed study was conducted to evaluate F1 hybrids at seedling subject to 45% moisture level. The data was recorded and subjected to multivariate analysis to find the significant variation attributed by various traits under stress conditions for efficient root/shoot development.
Results: Significant differences were found in F1 hybrids for all studied traits. Higher heritability was found for root length, shoot length and fresh shoot weight, while fresh shoot weight, dry shoot weight and dry root weight showed higher genetic advance. Significant correlation was found for dry root weight to fresh shoot length and fresh root length, fresh root weight to fresh root/shoot weight ratio and fresh shoot weight. The hybrids Sh-139×B-316, Raka-poshi×B-316, B-327×B-316, Sh-139×EV-340, EV-1097Q×EV-347, EV-1097Q×EV-340, EV-1097Q×Pop/209 and B-327×EV-340 showed higher and positive heterosis and heterobeltiosis for most of the studied traits. To assess the overall variation on dependent structure, we used multivariate analysis, an important tool in breeding program, for efficient selection.
Conclusions: EV-1097Q×Pop/209 and Sh-139×EV-340 showed significant results for root and shoot development under various water stress regimes at seedling stage, thus further studies should be carried out to find out the known and un-known loci regarding root and shoot development traits in high yielding maize cultivars under arid/semi-arid regions.
The RunningLine
2016-02-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/175
Advancements in Life Sciences; Vol 3, No 2 (2016): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/175/856
Copyright (c) 2016
oai:ojs.submission.als-journal.com:article/1147
2021-08-04T10:20:57Z
ALS:ART
nmb a2200000Iu 4500
"210804 2021 eng "
2310-5380
dc
Cloning and expression of hepatitis B surface gene in E. coli
Gulzar, Krishma
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Sharif, Maria
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Khan, Arif M
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Javed, Muhammad Rizwan
Department of Bioinformatics and Biotechnology (BNB), Government College University Faisalabad (GCUF), Faisalabad, Pakistan
Malik, Imran Riaz
Assistant Professor
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Molecular Biology
Background: Hepatitis B virus (HBV) is among the smallest DNA viruses resulting in ~800,000 deaths each year. Pakistan is considered a country affected by HBV. In Pakistan, the most dominant genotype is D. HBV is an enveloped virus of 3.2 kb. The study's goal was to express hepatitis B surface antigen in a bacterial host to produce a recombinant protein.
Method: Blood samples were collected in EDTA coated vacutainer from patients after their consent. DNA was extracted from serum through the phenol-chloroform method; Hepatitis B surface gene was cloned in TA cloning vector, subclone in pET 28a expression vector. An expression vector containing the Surface gene was then transformed into a competent bacterial host BL21 and inducted with IPTG at 0.1-0.2mM concentration for expression. The expressed proteins (soluble and pellet form) were analyzed on SDS PAGE.
Results: Hepatitis B Surface gene of 681bp after PCR were detected under UV light then successfully cloned and subcloned in pET 28 expression vector. The restricted fragment indicating the gene of interest was 681bp when analyzed on 1.2% Agarose gel under UV light. The required protein of 25kDa was obtained in soluble form when detected on 12% SDS PAGE after staining with Coomassie Blue dye.
Conclusion: Hepatitis B surface gene was successfully expressed in both insoluble and pellet forms using E.coli. The expression of surface protein needs to maximize through optimizing conditions to be used as potent candidate for vaccine production to prevent hepatitis B infection.
Keywords: Hepatitis B virus; Surface gene; Cloning vector; pET expression vector
The RunningLine
2021-08-04 10:20:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1147
Advancements in Life Sciences; Vol 8, No 3 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1745
2023-07-15T04:31:43Z
ALS:ART
nmb a2200000Iu 4500
"230715 2023 eng "
2310-5380
dc
Serological and Hematological Studies of the Blood of Calves in the Experimental Use of the Medication Triazavirin
Reshetnikova, Tatiana
National Research Mordovian State University named after N.P. Ogareva http://orcid.org/0000-0002-8591-8069
Krylova, Tatyana
Izhevsk State Agricultural Academy http://orcid.org/0000-0001-6239-0612
Background: In intensive animal husbandry management, the high disease occurrence in young animals remains an urgent problem, which is a direct consequence of the crowded placement of animals and insufficiently strong immune systems. The paper describes the study of the effect of the antiviral medication Triazavirin on the activity of humoral immunity and the level of specific antibodies in the blood serum of calves.
Methods: Hematological and serological studies were carried out. The experiment involved 40 calves of the black-and-white breed, aged one month, with an average weight of 50 kg, without clinical signs of diseases. For this experiment, two groups of calves were formed using the analog method. The animals in the first and second groups received the medication in dosages of 62.5 mg and 1,000 mg by inhalation and orally, respectively.
Results: In the first group, hematological studies showed the presence of leukocytosis, lymphocytosis, and agranulocytosis. When studying the level of antibodies in the blood serum, a decrease in the level of antibodies was discovered, which indicates a therapeutic effect and a decrease in the number of viral particles. The titer level of specific antibodies was at the same level, which was associated with taking the medication and activating adaptive mechanisms of the body's defense. In hematological studies of the blood of animals of the second group, leukocytosis was noted. When studying the level of antibodies in the blood serum, it was noted that the level of antibodies had decreased by 40%. The titer level of specific antibodies was at the same level.
Conclusion: The results obtained made it possible to recommend the use of the antiviral medication in question for the treatment of respiratory diseases in calves. The authors present conclusions on the effectiveness of the inhalation route of administration of the medication for viral pathogenic agent control.
Keywords: Calves; Antiviral medication; Blood; Antibody titer; Hematological; Serological studies
The RunningLine
2023-07-15 04:31:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1745
Advancements in Life Sciences; Vol 10, No 2 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/462
2018-11-27T02:30:53Z
ALS:ART
nmb a2200000Iu 4500
"180825 2018 eng "
2310-5380
dc
Detection and comparison of light metals in hair among workers of different industries using Particle Induced X-Ray Emission (PIXE) for forensic casework
Rehman, Muhammad
Forensic Medicine Diretorate, Forensic DNA Fingerprinting Laboratory, Ministry of Public Health, Kabul, Afghanistan
Waqas, Muhammad
Shahzad, Muhammad Saqib
Faculty of Life Sciences, University of Central Punjab
kalsoom, Saeeda
IMBB, University of Lahore
Bajwa, Javed Iqbal
Punjab Medical College, Faisalabad
Arif, Amina
Faculty of Life Sciences, University of Central Punjab
Khan, Muhammad Azmat Ullah
Faculty of Life Sciences
Forensics; Molecular Biology; Biotechnology
Background: The study was planned to evaluate the deposition of some metals in the scalp hair of the people working in the particular environment. Scalp hair samples were collected from different industry workers including pharmaceutical, textile and paint industry and analyzed for the determination of aluminum (Al), calcium (Ca), potassium (K) and sulfur (S). Hair analysis provides a better assessment of light metals present in the surroundings, as well as monitoring of variation of metals from place to place.
Methods: The hair samples were collected from 21-55 years old employees working in industry for more than four years. Workers of the age less than 20 years and less than 4 years working experience were not including in this study. Particle Induced X-ray Emission (PIXE) system was used to detect the light metals from hair and probable matches were searched through National Institute of Standards and Technology (NIST) library.
Results: The metals Al, S, K and Ca concentration was found maximum in the pharmaceutical industry workers. i.e., 575.1 µg/g, 190.7 µg/g, 11.1 µg/g and 9.1 µg/g respectively. The minimum concentration so these metals was found in paint industry workers i.e. 103.7 µg/g, 8.87 µg/g, 2.3 µg/g, and 1.7µg/g respectively.
Conclusion: Light metal concentrations in hair samples showed a significant positive correlation. Our findings can play vital role for health departments and industrial environmental management system (EMS) authorities in policy making and implementation. Taken all together, the workers were facing minor health implications in these industries and need immediate protective measures to remediate the current situation.
Keywords: Forensic, Scalp Hair Analysis, Particle Induced X-ray Emission
The RunningLine
2018-08-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/462
Advancements in Life Sciences; Vol 5, No 4 (2018): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/462/1623
Copyright (c) 2018 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1364
2022-05-29T08:45:07Z
ALS:ART
nmb a2200000Iu 4500
"220529 2022 eng "
2310-5380
dc
Antibacterial Evaluation of The Roots of Moroccan Aristolochia longa Against Referenced Gram-positive and Gram-negative Bacteria
Doudach, Latifa
Al-mijalli, Samiah H
Abdallah, Emad M
Mrabti, Hanae N
Chibani, Fatiha
Faouzi, Moulay El Abbes
Background: Interest in medicinal plants has recently risen as a consequence of its therapeutic capabilities, which require further investigation. Aristolochia longa (A. longa) roots are commonly employed in traditional Moroccan medicine as an anticancer agent and against some cutaneous infections, but little is known about their antibacterial capabilities.
Methods: The disc-diffusion and minimal inhibitory concentration (MIC) methods were carried out to determine the antibacterial activity of aqueous and methanol extracts of A. longa roots against several reference bacterial strains.
Results: Both aqueous and methanolic extracts had antibacterial activity to varying degrees with the disc-diffusion assay, however the methanolic extract shown significant activity at a higher dosage (200 mg/ml). Escherichia coli (22.0 mm) was the most sensitive bacterium tested, followed by Staphylococcus aureus (16.0 mm) and Bacillus subtilis (12.0 mm). The bacteria with the lowest susceptibility were Klebsiella pneumonia, Pseudomonas aeruginosa, and Micrococcus luteus, which all had a 10.0 mm zone of inhibition. The MIC values corroborated these observations, with E. coli MICs of 6.25±1.5 mg/ml, S. aureus MICs of 25±1.02 mg/ml, M. luteus MICs of 12.5±1.25 mg/ml, K. pneumonia MICs of 50.0±0.75 mg/ml, and B. subtilis MICs of 100±0.6 mg/ml, respectively. The findings of the methanolic extract were statistically significant and equivalent to those of the antibiotics studied.
Conclusion: Roots of A. longa have significant broad-spectrum antibacterial agents, notably in the methanolic extract, which validated their applications in Moroccan folkloric medicine to treat soft-tissue and skin infections.
Keywords: Aristolochia longa; Antibacterial activity; Aqueous extract; Methanolic extract; Disc-diffusion; Minimal inhibitory concentration
The RunningLine
2022-05-29 08:45:07
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1364
Advancements in Life Sciences; Vol 9, No 1 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1970
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Anti-calponin, anti-PCNA immunohistochemical study of the parenchymatous cells in mice parotid salivary glands exposed to dental radiograph
Muhammad, Firas Riyadh
Department of human anatomy- Medical college / Al-Nahrain university
Kadhim, Hayder Jawad
Department of human anatomy- Medical college / Al-Nahrain university
Al-Habib, May. F. M.
Department of human anatomy- Medical college / Al-Nahrain university
Background: Radiation therapy with ionizing radiation is associated with an increased risk of salivary gland cancer and xerostomia due to an abnormally high expression of the regulatory protein calponin. PCNA is a cofactor for DNA polymerase-delta and is involved in cell proliferation.
Methods: This study evaluated the anti-calponin and anti-PCNA immunohistochemical reactivity in the parotid salivary glands of mice after frequent exposure to dental X-ray.
Results: The results showed that exposure to radiation causes marked differences in PCNA reactivity. Radiation therapy has a significant impact on salivary glands, particularly on myoepithelial cells.
Conclusion: The exposure to radiation can cause Dental X-ray exposure increases the risk of developing cancer and other health problems due to damage to DNA and inflammatory destruction and further research is needed to mitigate these effects and improve patient outcomes.
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1970
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/849
2019-11-27T10:05:03Z
ALS:ART
nmb a2200000Iu 4500
"191127 2019 eng "
2310-5380
dc
Genetic contribution of GJB2 gene to hearing impairment in Pakistan
Tariq, Hamna
National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Zaigham, Kalsoom
National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Kousar, Samra
National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Azhar, Aysha
The University of Faisalabad (TUF), Faisalabad, Pakistan
Background: Hearing impairment (HI) is defined as inability to hear and is an extremely heterogeneous genetic disorder. HI is divided into syndromic (if associated with clinical manifestation in addition to hearing impairment) and non-syndromic forms. So far one hundred and seventeen loci/genes have been mapped for non-syndromic HI and mutations in DFNB1 locus (GJB2 gene) are the most prevalent cause among them. This study was intended to find the relative contribution of the DFNB1 locus/ GJB2 gene for hearing loss in Pakistan and Azad Kashmir.
Methods: Twenty-one families were collected from different rural and urban regions of Pakistan and Azad Kashmir. The contribution of GJB2 gene was initially studied by linkage analysis using short tandem repeats (STR) microsatellite markers. Sanger sequencing was employed to identify the causative variants in coding region of the gene.
Results: Phenotype of four families were found linked with GJB2 gene and all affected individuals of these families segregating same mutation c.231G>A (p.Trp77*) which was confirmed after Sanger sequencing.
Conclusion: The genetic causes of hearing impairment were studied in twenty one families segregating autosomal recessive pattern of inheritance with different ethnicities. We further established the founder effect for the one recurrent mutation in GJB2 gene in Pakistani and Kashmiri hearing impaired families for the very first time.
Keywords: DFNB1; GJB2; Hearing impairment; Pakistan and Kashmir
The RunningLine
2019-11-27 09:56:35
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/849
Advancements in Life Sciences; Vol 7, No 1 (2019): Advancements in Life Sciences
eng
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2239
2024-01-08T07:56:30Z
ALS:ART
nmb a2200000Iu 4500
"240108 2024 eng "
2310-5380
dc
Identification of potential inhibitors targeting DNA adenine methyltransferase of Klebsiella pneumoniae for antimicrobial resistance management: a structure-based molecular docking study
Alshehri, Ameerah Ali
Microbiology Department, Armed Forces Hospital, AlTaif, Saudi Arabia
Almutairi, Ayshah Musad
Armed Forces Center for Health Rehabilitation, AlTaif, Saudi Arabia
Shafie, Alaa
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia
Alshehri, Norah Ali
Department of Family and Community Medicine, College of Medicine, King Saud University (KSU), Riyadh, Kingdom of Saudi Arabia
Almutairi, Samia Musaad
Taif Directorate of Health Affairs, AlTaif, Saudi Arabia
Anjum, Farah
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Background: Klebsiella pneumoniae is an important opportunistic pathogen that frequently causes nosocomial infections. Notably, this bacterium has emerged as a major problem in hospital settings because of its acquisition of resistance to carbapenems. The majority of antibiotics act by targeting crucial pathways within bacterial cells. However, due to the development of resistance mechanisms, the efficiency of these antibiotics has decreased. Therefore, this study focused on a putative protein (DNA adenine methyltransferase; Dam) found in K. pneumoniae that encompasses a DNA methylation protein domain, indicating a novel potential target for pharmacological intervention. DNA methylation affects bacterial virulence attenuation.
Methods: In the unavailability of a 3D structure for Dam protein in protein database, a 3D model was generated using SWISS-MODEL server and validated using computational tools. Following that, screening was performed against the Dam protein using a set of 2706 phytochemicals obtained from the ZINC database using PyRx0.8. ProTox-II platform was used to predict the physicochemical properties and various toxicity endpoints.
Results: Among the screened compounds, ZINC4214775, ZINC4095704, and ZINC4136964 had higher binding affinity for the Dam and interacted with its active site residues. The computational analyses of these three identified hits indicate that their predicted properties were within an acceptable range for evaluating toxicity. In addition, a toxicity radar chart showed that these hits were within an acceptable range.
Conclusions: These compounds have the potential to act as Dam inhibitors and could be investigated further for managing antimicrobial resistance in K. pneumoniae.
The RunningLine
2024-01-08 07:56:30
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2239
Advancements in Life Sciences; Vol 10, No 4 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/22
2014-06-13T04:44:26Z
ALS:ART
nmb a2200000Iu 4500
"140225 2014 eng "
2310-5380
dc
Occurrence of HCV genotypes in different age groups of patients from Lahore, Pakistan
Rasheed, Amna
Allama Iqbal Medical College/Jinnah Hospital, Lahore
Ullah, Sajjad
Allama Iqbal Medical College/ Jinnah Hospital, Lahore
Naeem, Sajid
Govt. College University
Zubair, Muhammad
University of Lahore
Ahmad, Waseem
University of Lahore
Hussain, Zahid
Govt. College University
microbiology; health microbiology; virology; epidemiology
Background: Hepatitis C virus is a small, enveloped single stranded, positive sense RNA virus. Different genotypes are distributed in different geographical areas of the world. Determination of HCV genotype is a powerful tool for the treatment of chronic and acute liver disease.
Method: The present study was carried out to find the occurrence of different HCV genotypes in the city of Lahore, a populous city of Pakistan from January 2010 to December 2010. Blood sample of patients positive for anti HCV by ELISA as well as HCV by PCR were collected and plasma was separated. HCV viral RNA load was analyzed in these samples using Real Time PCR. Qiagen HCV mini kit for RNA extraction and Qiagen HCV amplification kit for PCR amplification were used. Amplicons were subjected to HCV genotyping using Third Wave Technology.
Results: Among 489 patients, 211 (43.1%) patients were males and 278 (56.9%) were females. Occurrence of HCV in the age group of 36-45 years was 32.5 %. Occurrence of HCV genotype 1 was 9.6% (47), genotype 3a was 80.77% (395), genotype 3h was 1.0% (5) , genotype 4 was 4.9% (24), co-infection of genotypes 1 & 2 was 0.2% (01), co-infection of genotypes 1 & 3 was 0.6% (03) and co-infection genotypes 1 & 4 was 0.4% (02).
Conclusion: HCV genotype 3a is most prevalent HCV genotype in subjected population during said duration with most infected people from 26 to 35 years of age. Female population is having more of HCV infection as compared to males.
The RunningLine
2014-02-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/22
Advancements in Life Sciences; Vol 1, No 2 (2014): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/2533
2024-03-11T07:54:49Z
ALS:ART
nmb a2200000Iu 4500
"240225 2024 eng "
2310-5380
dc
Molecular Genetic Assessment of Nodular Dermatitis Virus in Cattle Herds
Gnezdilova, Larisa
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Laga, Vita
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Marzanova, Saida
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Yarygina, Elena
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Pozyabin, Sergej
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Selina, Marina
Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I.
Skryabin
Background: The study highlights the significance of data availability in molecular genetic research, focusing on cattle nodular dermatitis virus. Limited data on Russian genetic variants hinders comprehensive virus characterization. Molecular genetic methods are crucial for accurate diagnosis, considering cross-reactions with poxviruses. Increased data availability is essential to improve diagnostics and biosafety in the region.
Method: The researchers conducted a molecular genetic analysis of the gene sequences of the nodular dermatitis virus to assess the variability of the virus. The authors used the GenBank database to compare sequences and used the MEGA X program for phylogenetic analysis and sequence alignment.
Results: The sequences of the nodular dermatitis virus closest to the Russian genetic variants have been determined. The volumes of available epizootiological and molecular genetic data were compared, and the circulation of the vaccine strain was confirmed.
Conclusion: The article emphasizes the need to increase the availability of data on the genetic sequences of Russian samples to fully characterize the genetic diversity of the nodular dermatitis virus. Despite the limited data, the authors observed a tendency to increase genetic diversity. They recommend isolating and storing the virus in the cell culture to enhance genetic information.
Keywords: Databases; Epizootiology; Molecular Genetic Analysis; Nodular Dermatitis
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2533
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/149
2015-08-24T14:34:29Z
ALS:ART
nmb a2200000Iu 4500
"150825 2015 eng "
2310-5380
dc
Assessment of salinity tolerance in rice using seedling based morpho-physiological indices
Zafar, Syed Adeel
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Shokat, Sajid
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
Ahmed, Hafiz Ghulam Muhu-Din
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Khan, Adeel
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Ali, Muhammad Zeshan
Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi, Pakistan.
Atif, Rana Muhammad
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Biotechnology, Agriculture
Background: Salinity is among the most damaging abiotic stresses for rice production which limits its growing area. The present research was conducted to evaluate five rice varieties for salinity tolerance at seedling stage.
Methods: Experiment was conducted in triplicate and in two sets. One set was grown as a control (non-stress) and other as salt stressed. Salt stress of 15 dS/m was applied to one set of rice seedlings under controlled conditions. Data for different growth related morpho-physiological traits, i.e. germination percentage, root and shoot length, seedling fresh and dry weight, Na+ and K+ uptake were recorded after 15 days of seedling emergence under control as well as salinity condition.
Results: Significant differences were observed among the genotypes under both the treatments and interaction of the evaluated traits suggested a significant variability among the rice genotypes under salt stress. NIAB-IRRI-9, Basmati-198 and KSK-133 were proved to be relatively salt tolerant varieties as they showed good performance for the recorded parameters. However, Basmati-385 was observed a salt sensitive variety due to highest reduction in seedling fresh and dry weight along with the maximum Na+ uptake.
Conclusion: Based on obtained results, it was concluded that the evaluated morpho-physiological traits were useful to screen rice cultivars for salinity stress. In addition, NIAB-IRRI-9, Basmati-198 and KSK-133 can be used in breeding programs as tolerant check and Basmati-385 can be used as sensitive check.
The RunningLine
2015-08-24 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/149
Advancements in Life Sciences; Vol 2, No 4 (2015): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/1119
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
Impact of Sickle Cell Anemia on children growth and clinical parameters in Al-Ahsa region of Saudi Arabia
Alanazi, Nawaf
Dr Nawaf Alanazi
Pediatric Hematology / Oncology Division, Department of Pediatrics, King Abdulaziz Hospital, Al-Ahsa, Saudi Arabia.
Clinical Laboratory Sciences Program, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences / KAIMRC/ SSBMT, King Abdulaziz Medical City, National Guard Health Affairs, Al-Ahsa, Saudi Arabia
Alabdullatif, Shahad
Albahrani, Maryam
Aljamaan, Malak
Alsayegh, Fatimah
Clinical Laboratory Sciences Program, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences / KAIMRC/ SSBMT, King Abdulaziz Medical City, National Guard Health Affairs, Al-Ahsa, Saudi Arabia
Bhalli, Aysha
Aljarrah, Khaled
Iqbal, Zafar
Director, Clinical Laboratory Sciences (CLAB) Program
Chairman, CLAB Unified Curriculum Committee, CoAMS
Chairman, CLAB Program Accreditation Sub-committee (CLAB-PAS)
Associate Professor (Molecular Genetics / Clinical Hematology & Oncology)
College of Applied Medical Sciences (CoAMS), King Saud Bin Abdulaziz University for Health Sciences
National Guard Health Affairs, Mail Code 501/502, P.O. Box 2477 Al Ahsa, 31982, Saudi Arabia.
Phone: (Office) +966 13 5628303, (Cell) +966 53 86 355 86; Whatsapp: +1-202-445-5907
E-mail: iqbalz@ksau-hs.edu.sa ; iqbalza2@ngha.med.sa; drzafar.medgen@yahoo.com
Sickle cell disease; growth; clinical complications; Saudi Arabia; Eastern Province
Background: Sickle cell disease (SCD) is an autosomal recessive disease caused by a single gene mutation, leading to sickle-shaped red blood cells, causing many clinical complications. Resulting complications may affect the growth of the SCD patients that is a strong measure of severity of disease and helps in disease management strategies in any area. Eastern province of Saudi Arabia has one of the highest SCD incidences. Nevertheless, no studies have been previously carried out of about clinical outcome of SCD in Al-Ahsa area of eastern province. Therefore, this study was conducted to find out the impact of SCD children at king Abdulaziz Hospital Al-Ahsa.
Methods: All pediatric SCD patients were included in the study. Patient data was taken from hospital information system and analyzed using SPSS version 27.
Results: A total of 53 patients were studied. The male to female ratio was 1.4:1 and mean age was 3.3 years (range: 1-9). Eighteen (34%) did not present with sickle cell crisis possibly due to ameliorating effects of high HbF and G6PD deficiency. Although growth parameters of SCD patients were not statistically different from international standards, there was significant difference between weight of SCD patients in recurrent sickle cell crisis group and non-crisis sickle cell (NC-SC) group at diagnosis and after clinical interventions (p= 0.04 and 0.03, respectively) that included hydroxyurea. The corrected reticulocyte (at diagnosis and after clinical intervention) and WBC counts were statistically significant between hydroxyurea and non-hydroxyurea groups (p-value < 0.05).
Conclusions: Overall, one-third of SCD patients in Al-Ahsa region have mild disease and hydroxyurea can minimize the SCD severity through lowering corrected reticulocyte and WBC counts. Exact mechanisms of mild SCD and hydroxyurea in minimizing disease severity are needed to be elucidated.
Keywords: Salinity; Livelihood; Productivity; Farming experience; Farm size
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1119
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1554
2023-07-15T04:31:43Z
ALS:ART
nmb a2200000Iu 4500
"230715 2023 eng "
2310-5380
dc
Effect of quinoa biomass and biocontrol fungi on expression of IPER gene in mung bean in Macrophomina phaseolina contaminated soil
Chaudhury, Farman Ahmad
School of Food Sciences and Technology, Minhaj University Lahore, Pakistan
Khan, Iqra Haider
Department of Plant Pathology, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan
Javaid, Arshad
Department of Plant Pathology, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan
Background: Mung bean is a pulse crop of economic significance, grown in Pakistan for its edible seeds. Its production is severely affected by Macrophomina phaseolina, a necrotrophic pathogen. This study was carried out to investigate the effect of different biocontrol fungi on expression of IPER gene in mung bean plants inoculated with M. phaseolina.
Methods: Pot trials were carried out by adding different concentration of dry biomass of quinoa (DBQ) and six antagonistic fungi viz. Aspergillus flavipes, Aspergillus versicolor, Penicillium digitatum, Penicillium italicum, Trichoderma pseudokoningii and Trichoderma viride in M. phaseolina infected soils. After four weeks of germination of mung bean seeds, RNA was extracted from roots and leaves by using TRIzol method and cDNA was prepared by using SuperScriptTM IV First-Strand Synthesis Kit. IPER gene expression was studied on qRT-PCR and ACT was used as a housekeeping gene.
Results: The expression of IPER gene was higher in positive control (only inoculated with M. phaseolina) than in negative control (no amendment). Moreover, 1 and 2% doses of DBQ showed slightly higher Ct values than in 3% dose where it was dropped down indicating the allelopathic stress of DBQ treated soils. The treatments either with sole application of antagonistic fungi or together with DBQ showed much higher Ct values indicating no stress at all.
Conclusion: Plants only inoculated with M. phaseolina showed the highest disease incidence as compared to the negative control. The soils amended with DBQ and antagonistic fungi significantly alleviated the effect of M. phaseolina. The treatments with increased pathogen stress showed reduced Ct values and vice versa.
Keywords: Biocontrol; IPER gene; Gene expression; Macrophomina phaseolina; Mung bean
The RunningLine
2023-07-15 04:31:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1554
Advancements in Life Sciences; Vol 10, No 2 (2023): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/1554/7143
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/558
2018-02-25T00:00:19Z
ALS:ART
nmb a2200000Iu 4500
"180225 2018 eng "
2310-5380
dc
Genome-Wide Identification of Natural Selection Footprints in Bos Indicus Using Principal Component Analysis
Mustafa, Hamid
University of Veterinary and Animal Sciences Lahore.
Khan, Waqas Ahmed
University of Sargodha, Sargodha
Sonstegard, Tad
Li, Yi
Ain, Noor Ul
Ajmal, Adeela
Molecular Biology; Genomics; Animal Sciences
Background: To describe natural selection, numerous analytical methods for ascertaining candidate genomic region have been developed. There is a substantial drive in population genomics to identify loci intricate in local adaptation. A potent method to find genomic regions subject to local adaptation is to genotype numerous molecular markers and look for outlier loci.
Methods: In this study, population structure and genome wide footprints scan of natural selection in cattle was performed using principal component analysis based on alternative individual method assumed in the PCAdapt R-package. This method was used on the hypothesis that extremely related population markers are also local population adaptation candidates. To test PCAdaptmethod in cattle, the data of sixty three animals were collected from four different origins or agro-ecological zones (Achai = 18, Cholistani = 13, Lohani = 19, and Tharparkar = 13) and genotyped using the high density SNPs BeadChip.
Results: As expected from the sampling from different zones the principal component result indicated the clear division in these animals into three clusters. K=3 was the optimal number suggested by eigenvalues.
Conclusion: The result of this study revealed that the genomic regions harboring signals of the candidate genes were associated with immunity system and muscle formation. Signature of selection detecting in this study targeted the historical adaptation in these breeds that will be useful in future to understand cattle origin under different environment.
The RunningLine
2018-02-25 00:00:19
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/558
Advancements in Life Sciences; Vol 5, No 2 (2018): Advancements in Life Sciences
eng
Copyright (c) 2018 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1145
2022-05-29T08:45:07Z
ALS:ART
nmb a2200000Iu 4500
"220529 2022 eng "
2310-5380
dc
A study on serological detection of Hepatitis A virus with associated risk factors in young kids
Khan, Aysha Iqbal
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore
Ali, Rana Haider
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore
Siddiq, Hafiz Muhammad Usman
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore
Irfan, Suleman
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore
Siddiq, Hafiz Muhammad Usman
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore – Pakistan
Fatima, Noor
Faculty of Veterinary sciences, University of Veterinary and Animal Sciences, Lahore
Zafar, Ramla
Star Produce (Food processing company), Kelowna, British Columbia
Health and Epidemiological studies
Background: Hepatitis A virus (HAV) is a major concerning issue for human health that causes acute viral hepatitis. Hepatitis A virus is non enveloped RNA virus which is a member of the family Picornaviridae and genus Hepatovirus. HAV is more prevalent in developing countries like Pakistan with poor sanitation and economic status. The virus is present in young kids of age 1 to 15 years old associated with many risk factors.
Methods: A total of 100 blood samples (1-2 ml) were collected from the hospitals of Lahore. After serum collection, indirect ELISA was performed on a commercially available kit. The Optical density (OD) was taken from the ELISA reader. Positive and negative samples were also run along with the samples and samples OD was compared with the OD of positive and negative controls. Cut off value was calculated by multiplying negative control (NC) with 2.1. The absorbance value of specimen/cutoff <1: samples having a value less than cut-off value were considered negative and samples having a value greater than or equal to cut-off value were considered positive. The apparent prevalence of Hepatitis A virus was measured by dividing the number of children positive to the total number of children included in the study.
Results: Of the 100 samples tested, 37% were positive for IgM and 100% were positive for IgG. Statistical Analysis SPSS 21.0 version was applied to analyze the data and a correlation test was applied to see the association of risk factors with disease status.
Conclusion: This study was done to appraise the overall status of HAV prevalence in young kids in association with potential risk factors.
Keywords: Hepatitis A; ELISA; Risk assessment; Prevalence; Hygiene
The RunningLine
2022-05-29 08:45:07
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1145
Advancements in Life Sciences; Vol 9, No 1 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2298
2023-11-26T07:12:45Z
ALS:ART
nmb a2200000Iu 4500
"230930 2023 eng "
2310-5380
dc
Anticoccidial Effect of Cinnamomum verum Essential Oil and Its Impact on Hematological and Serum Biochemical Parameters in Broilers
Al Syaad, Khalid M.
Department of Biology, College of Science, King Khalid University, Abha 61413 - Saudi Arabia
Salman, Muhammad
Department of Parasitology, University of Agriculture Faisalabad, Pakistan
Abbas, Rao Zahid
Department of Parasitology, University of Agriculture Faisalabad, Pakistan
Khan, Muhammad Kasib
Department of Parasitology, University of Agriculture Faisalabad, Pakistan
Mahmood, Muhammad Shahid
Institute of Microbiology, University of Agriculture Faisalabad, Pakistan
Veterinary Sciences
Background: There are various synthetic anticoccidial drugs available in the market for the control and treatment of coccidiosis in broilers. However, their extensive usage has resulted in the development of drug resistance as well as the presence of drug residues in meat, thus urging scientists to find alternatives for coccidiosis control. Hence, the current research was aimed at the evaluation of the anticoccidial potential of Cinnamomum verum essential oil through the application of both the in-vitro and in-vivo methods.
Methods: The bark of C. verum procured from the market was subjected to hydro-distillation procedure for extraction of the essential oil. The extracted essential oil was subjected to in-vitro evaluation in terms of percent sporulation and oocysts damage at six different concentrations (0.31, 0.625, 1.25, 2.5, 5 and 10% v/v). Similarly, for the in-vivo trial, 72 broiler chicks were randomly divided into six equal groups (A, B, C, D, E and F). The first five groups were infected with oocysts of mixed Eimeria species while the sixth group was kept as non-infected. When the chicks were 14 days old, the infected groups were orally given 55000 oocysts per bird. On the same day 14, the groups A, B and C were given C. verum essential oil at concentrations of 1, 2 and 3% respectively in feed whereas groups D, E and F served as positive control (Toltrazuril® treated), negative control and the normal control respectively.
Result: The results revealed C. verum oil to have an effect on the percent sporulation and oocysts damage. The oil also improved the FCR, lesion score, oocysts score, fecal score and serum biochemical parameters in the treated broilers. However, it had no significant positive effect on the hematological parameters like Hb, PCV and blood cells count, and the weight of internal organs in broilers. For most of the parameters, C. verum essential oil showed a dose-dependent effect.
Conclusion: In nutshell, C. verum essential oil possesses significant anticoccidial potential as demonstrated by the results of both the in-vitro and the in-vivo experiments. However, further studies are required for its validation and commercialization in the poultry sector.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2298
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/816
2019-08-25T00:54:00Z
ALS:ART
nmb a2200000Iu 4500
"190825 2019 eng "
2310-5380
dc
Detection of Human Salivary Amylase Level Deposited on Fruits with First Bite Mark
Draz, Umar
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore
Ali, Saadat
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore
Firyal, Sehrish
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore
Ali, Ahmad
National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre (NARC), Islamabad
Saleem, Ali Haider
Department of Animal Sciences, College of Veterinary and Animal Sciences, Jhang
Tahir, Mohammad Ashraf
Punjab Forensic Science Agency, Lahore
Molecular Biology; Biochemistry; Biotechnology
Background: Human body fluids are vital for criminological examinations. The recovery, conservation, and investigation of body fluids or its stains are critical in measurable forensic cases. The amylase testing is being utilized as a possible test to locate the crime scene saliva stains for more than three decades. This investigation was intended to decide the human salivary amylase accumulated on fruit after the first bite where the level of salivary amylase was concluded by Radial Gel Diffusion method.
Methods: Three sorts of organic fruits (apple, peach, and apricot) and 20 people (10 male and 10 female) were chosen for this investigation. The specimens were assembled from bite mark area following time intervals 0 hours, 12 hours, 24 hours, 36 hours and 48 hours.
Results: Experimental outcomes demonstrated that the diameter of amylase activity in male specimens was more noteworthy than female. Apples, as well as apricot pit samples, indicated amylase activity till 48 hours; however, the specimens collected from peach demonstrated amylase activity until 12 hours, after first bite mark.
Conclusion: This study demonstrated that salivary amylase testing might be valuable for DNA evidence, where DNA investigations for every case might be restricted because of cost issues.
Keywords: Amylase; Saliva; Investigation; Bite mark; Preservation
The RunningLine
2019-08-25 00:54:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/816
Advancements in Life Sciences; Vol 6, No 4 (2019): Advancements in Life Sciences
eng
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2316
2024-01-09T15:54:21Z
ALS:ART
nmb a2200000Iu 4500
"231231 2023 eng "
2310-5380
dc
Segmental Structure of the Human Kidney: An Innovative Research Algorithm
Kafarov, Edgar
Chechen State University
Avduev, Imran
Chechen State University
Lechiev, Zelimkhan
Chechen State University
ackground: Understanding the anatomical structure of the human kidney, particularly its zonal and segmental organization, is crucial for both theoretical knowledge and practical applications in surgical procedures. That is why the purpose of the article was to study the variants of the zonal and segmental structure of human kidneys.
Methods: 116 corrosive preparations of the arterial system of the human kidney served as the material for the study. The authors identified their extra-organ branches: a) the number of arterial vessels in the kidney hilum; b) the topographic and anatomical features of the renal artery trunks. In a 3D projection, zones of local zonal blood supply to areas of the renal parenchyma were identified, depending on the di- and trichotomic variants of the division of the main renal artery, A. renalis (I).
Results: It was found that some lines of the passage of the zones of natural divisibility of the kidneys with different variants of the division of A. renalis (I). There were from 5 to 7 (6 ± 1) segments in the kidneys on average. It is possible that if a four-zone blood supply system with corresponding zones of natural kidney divisibility is found in the kidneys, where the zones mostly don’t coincide with the boundaries of the passage of segments in their classical version, the number of segments will be even greater.
Conclusion: The results of the conducted study show that considering the generally accepted zones and boundaries of the passage of segments with zones of natural divisibility, which were not previously considered, the kidneys have an individual segmental structure, which requires revision. The authors believe that the generally accepted classical five-segment kidney model is outdated.
The RunningLine
2024-01-08 07:56:30
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2316
Advancements in Life Sciences; Vol 10, No 4 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/43
2014-04-07T04:27:00Z
ALS:ART
nmb a2200000Iu 4500
"131125 2013 eng "
2310-5380
dc
TH-6: a high yielding cultivar of sesame released for general cultivation in Punjab
Anwar, Muhammad
Assistant Research Officer, Oilseeds Research Institute, AARI, Faisalabad
Hasan, Ejaz-ul-
Assistant Research Officer, Oilseeds Research Institute, AARI, Faisalabad
Bibi, Tahira
Assistant Research Officer, Oilseeds Research Institute, AARI, Faisalabad
Bin Mustafa, Hafiz Saad
Assistant Research Officer, Oilseeds Research Institute, AARI, Faisalabad
Mahmood, Tariq
Botanists, Oilseeds Research Institute, AARI, Faisalabad
Ali, Muhammad
Botanists, Oilseeds Research Institute, AARI, Faisalabad
Background: Sesame (Sesamum indicum L.) is a diploid species, known as Beniseed, Gingelly, Sim-Sim and Til. It is grown in tropical and subtropical areas of the world. Sesame seed contains high nutritional values of certain compounds (25% protein and 50% oil) and is a vital source of natural antioxidants. TH-6 (96006) is characterized by white, bold and high yielding seeded variety of sesame with a unique character of having single stem.
Methodology: Variety Punjab Til-90 and line 436602 were cultivated at Oilseeds Research Institute, Faisalabad. At flowering, Til-89 was crossed as female with the line 436602 in 1990. In 1991, the F1 generation of plants were grown and self-pollinated at flowering. Homozygous progenies of pedigree number F6-96006 from F6 were evaluated for their high yield in season 1996-97. Evaluation was done through micro, zonal, station and finally “National Uniform Yield Trials”.
Results: In 2006, 2217 kg/ha production was marked as its maximum potential at Oilseed Research Institute (Faisalabad) in Zonal Varietal Trail. In National Uniform Sesame Yield 2006 and 2007 the performance of TH-6 was better than TS-3 (check) by giving 32.16% and 9.59% more yield respectively. TH-6 also showed moderate tolerance against insect pests Phyllody diseases and Charcoal Rot.
Conclusion: Agronomic studies revealed that TH-6 performed better if planted in 15th to 30thJune if supplementation of one bag of di-ammonium phosphate (DAP) and half bag of Urea at the time of sowing and flowering, respectively, with 1st and 2nd irrigation.
The RunningLine
2013-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/43
Advancements in Life Sciences; Vol 1, No 1 (2013): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/971
2020-09-03T04:55:48Z
ALS:ART
nmb a2200000Iu 4500
"200902 2020 eng "
2310-5380
dc
Development of a diagnostic scar marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp
Tan Quang, Hoang
Laboratory of Gene Technology, Institute of biotechnology, Hue Univeristy, VietNam
Thi, Pham Thi Diem
Lan, Tran Thuy
Huy, Nguyen Duc
Tram, Nguyen Duy Quynh
Lien, Nguyen Thi Thu
Molecular biology; Biotechnology
Background: In a previous report, we showed that Vibrio shilonii was found on whiteleg shrimp (Litopenaeus vannamei) with acute hepatopancreatic necrosis disease in Thua Thien Hue province, Vietnam. This study was performed to develop a diagnostic molecular marker generated by random amplified polymorphic DNA (RAPD) for V. shilonii rapid detection.
Methods: Pathogen Vibrio spp. were isolated from shrimps and fishes, and were identified by 16S rRNA sequencing. Genetic diversity of Vibrio strains was analysis by RAPD technique. Specific PCR product for V. shilonii was cloned and sequenced. SCAR marker was developed from specific PCR product.
Result: Twenty random primers were evaluated for RAPD to identify DNA polymorphisms between Vibrio species. The random primer OPN-06 generated a 468-bp DNA fragment specific for V. shilonii. This was then converted into a sequence-characterized amplified region (SCAR) marker designated N6-441.
Conclusion: Specific primers (Vshi-441F/Vshi-441R) amplified a unique DNA fragment in all V. shilonii isolates but not in the other Vibrio spp. This PCR assay showed significantly sensitive to the target DNA and reliably for the amplification the V. shilonii genome.
Keywords: AHPND; RAPD; SCAR; Vibrio shilonii; Vietnam
The RunningLine
2020-09-02 12:24:04
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/971
Advancements in Life Sciences; Vol 7, No 4 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1498
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Mixed Bacteriological Isolation Percentages from the Uterus of Cows Slaughtered due to Infertility
Risvanli, Ali
Kyrgyz-Turkish Manas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Bishkek, Kyrgyzstan http://orcid.org/0000-0001-5653-0025
Kalender, Hakan
University of Firat, Faculty of Veterinary Medicine, Department of Microbiology, 23100, Elazig, Turkey http://orcid.org/0000-0003-4653-1942
Safak, Tarık
University of Kastamonu, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 37100, Kastamonu, Turkey http://orcid.org/0000-0002-6178-4641
Yüksel, Burak Fatih
University of Firat, Faculty of Veterinary Medicine, Department of obstetrics and gynecology, 23100, Elazig, Turkey http://orcid.org/0000-0002-7256-9189
Karagulle, Burcu
University of Firat, Faculty of Veterinary Medicine, Department of Microbiology, 23100, Elazig, Turkey http://orcid.org/0000-0002-6628-4515
Yılmaz, Oznur
University of Siirt, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 56100, Siirt, Turkey http://orcid.org/0000-0003-0424-9471
Kilinc, Mehmet Akif
University of Bingol, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 12100, Bingol, Turkey http://orcid.org/0000-0003-1577-1556
Veterinary medicine; Veterinary Microbiolgy; Veterinary Obstetrics and Gynecology
Background: Intrauterine mixed infections are an important problem in cattle breeding. In this study, we aimed to determine the mixed bacteriological isolation rates from the uterus of cows with clinical metritis and to reveal the relationships between the bacteria that isolated together.
Methods: For this purpose, sterile swabs were taken from 490 uterus obtained from cattle slaughtered due to infertility in three slaughterhouses and used them to perform microbiological tests. After evaluating the data, it was determined that the bacterial isolation rate from uterus with clinical metritis was 76.14% (n = 268).
Results: The rates for 1, 2, 3, 4, and 5 bacteria isolated from the uterus with clinical metritis were 48.13%, 33.96%, 15.30%, 2.24%, and 0.37%, respectively. The isolation rates for Trueperella pyogenes, Fusobacterium necrophorum, and Escherichia coli alone from the uterus with clinical metritis were 27.08%, 28.00%, and 16.51%, respectively. In uterus with clinical metritis, Staphylococcus aureus (16.67%) was the most commonly isolated bacteria with T. pyogenes, Clostridium spp. (16%) co-isolated with F. necrophorum, and coagulase-negative staphylococci (16.51%) and Bacillus spp. (16.51%) were detected with E. coli.
Conclusion: As a result, it was concluded that when evaluating the microbiological results for cows with clinical metritis, mixed infections should be taken into more consideration, and the characteristics of the bacteria that isolate together should be considered during the treatment of mixed infections.
Keywords: Cattle; Uterine; Isolation; infertility
The RunningLine
2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1498
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/1498/6753
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2335
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
Validity of anti-nuclear antibodies, double strand DNA antibodies and other parameters in autoimmune hypoparathyroidism
Al-Ammiri, Hind H.
Dept. of Microbiology, College of Veterinary Medicine, University of Baghdad. http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
Hasan Al-Tai, Tharwa H.
Clinical Communicable Diseases Research Unit /College of Medicine/University of Baghdad. http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
Mahdi, Ali A.A.
Medical Lab. Science Technology, College of Health and medical Technology / Middle Techincal University. http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
Molecular Biology, Genomics
Background: Hypoparathyroidism is the destruction of the parathyroid gland by the immune system, as part of a multiple endocrine insufficiency syndrome. Tissue resistance to PTH's activities can occur in rare cases, resulting in hypoparathyroidism with increased PTH levels. Hypothyroidism is more common in females than males. 1,25-dihydroxyvitamin D3 (active vitamin D) levels must be evaluated to rule out vitamin D deficiency as a contributor to or cause of hypocalcemia. Hypocalcemia and phosphatemia are common symptoms of hypoparathyroidism. Antinuclear antibodies (ANA) and double strand DNA (DsDNA) are the important biomarkers for diagnosis and prognosis of patients with autoimmune diseases. ANA and DsDNA occur in different endocrine disorders including thyroid disorders like hypoparathyroidism.
Methods: Thirty Hypoparathyroidism patients with age range 21-74 years with (20 female and 10 male) were attending international Baghdad Medical Hospital, during the period from December 2019 to February 2021 and thirty apparently healthy were chosen as healthy control groups respectively. For these two study groups, the blood samples were collected to evaluate the serum level of serum phosphorus, calcitonin, Calcium, Vitamin D3, DsDNA and ANA. The type of kits used for serum DsDNA and ANA in humans were an indirect enzyme immunoassay (ELISA) and the kits for serum level of serum Vitamin D3 and calcitonins were a sandwich ELISA. Finally, these kits for the calcium and phosphorus inorganic Colorimetric method were used.
Results: Using Student's t-test, the present study's data revealed a statistically significant difference in the mean of age group cases and controls (P=0.005), as well as a statistically significant difference in serum mean values of each Serum phosphorus (P=0.005), calcitonin (P=0.005), calcium (P=0.002), and vitamin D3 centration (P=0.005) between these two groups . By using Chi-square test sex was not significant between hypoparathyroid and control (P=0.787) and DsDNA was not significantly more positive in hypoparathyroid subjects than control (P=0.112); while ANA was significant (P=0.024).
Conclusion: The study sheds light on the necessity to screen the hypo parathyroid patients for vitamin D, calcium and phosphorous levels and calcitonin for better prognosis of patients and for each of antinuclear antibodies (ANA) and double strand DNA (DsDNA).
Keywords: Hypoparathyroidism; Phosphate; Calcium; Calcitonin; Vitamin D; ANA; DsDNA
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2335
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/110
2015-02-25T00:00:25Z
ALS:ART
nmb a2200000Iu 4500
"150225 2015 eng "
2310-5380
dc
Interleukin 10 (IL-10) promoter-1082 A>G polymorphism and risk of cancer: Meta-analysis
Khan, Muhammad Tahir
mohammad ali jinnah university, islamabad http://www.jinnah.edu.pk/
Afzal, Sahar
Rehman, Ashfaq Ur
Zeb, Tariq
Molecular Biology, genomics
Background: Interleukin-10-1082A>G variant is the most widely investigated polymorphism in the IL-10 gene in cancer susceptibility. A number of case control analysis showed the link between IL-10-1082A >G polymorphism and cancer risk in humans population.
Methods: Twenty three case–control eligible studies, comprising 4753 cases and 6086 controls were selected for the meta-analysis of IL-10-1082 A/G SNP. The statistical analysis was conducted with comprehensive meta-analysis (CMA). We adopted heterozygous (GG vs. AG) model. Odds ratio (OR) with 95% confidence interval (CIs) was calculated to measure the power of the link under heterozygous model (GG vs AG).
Result: Overall result obtained under fixed effect model are [OR: 1.066, 95% CI: 0.989-1.267, P: 0.464].
Conclusion: Our meta-analysis indicates that IL-10 promoter-1082 polymorphism under fixed effect model is not associated with the overall risk of developing cancer, HCV, Bechet’s disease and diabetes type-2.
The RunningLine
2015-02-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/110
Advancements in Life Sciences; Vol 2, No 2 (2015): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/1001
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
Rh alleles and phenotypes among Saudi women in Hail Region, Saudi Arabia
Alcantara, Jerold C.
Department of Clinical Laboratory Sciences College of Applied Medical Sciences
University of Hail, Hail, KSA. 2240
Alcantara, Tessie Y.
Department of Clinical Laboratory Sciences College of Applied Medical Sciences
University of Hail, Hail, KSA. 2240
Al Shaghdali, Khalid
1Department of Clinical Laboratory Science, College of Applied Medical Sciences, University of Hail, Saudi Arabia
Alshammari, Fawaz D
Department of Clinical Laboratory Sciences College of Applied Medical Sciences
University of Hail, Hail, KSA. 2240
Al Shammari, Fahad Ghali M
2Clinical Laboratory Department, Hail Maternity and Children Hospital, Saudi Arabia
Health and Epidemiological studies
Background: The Rh system is considered as the most complex among the human blood group systems, with 61 antigens identified to date. This study aimed to provide preliminary data on the distribution of Rh alleles and phenotypes among Saudi women and compare them with other ethnic groups.
Methods: This retrospective cross-sectional study was conducted among Saudi women who visited the Maternity and Children Hospital of Hail from November 2019 to March 2020. A fully automated blood bank analyzer was used in determining the Rh subgroups (D, C, c, E, e) and phenotypes. Inferential statistics and chi-square tests were used appropriately for comparisons.
Results: The study included a total of 500 Saudi female patients. The most prevalent antigen found was the “e” antigen, while phenotype CcDee has shown to have the highest frequency. A significant difference exists in comparison with the other studies from various ethnic groups.
Conclusions: The prevalence and distributions of Rh alleles and phenotypes among Saudi women were revealed in this study. The findings showed that Rh alleles and phenotypes are diverse across various races and regions globally.
Keywords: Rh allele; Rh phenotype; Rh system; Saudi Arabia
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1001
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1654
2023-04-14T10:29:04Z
ALS:ART
nmb a2200000Iu 4500
"230414 2023 eng "
2310-5380
dc
T-cell Imbalance or Decreased Th:Tc Ratio in Immune Thrombocytopenia: Is it Clinically Significant?
Arshad, Aisha
1. Department of Transplant Immunology and Applied Microbiology, National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi – Pakistan
2. Department of Clinical Hematology, National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi – Pakistan
Mukry, Samina Naz
National Institute of Blood Diseases & Bone Marrow Transplantation http://orcid.org/0000-0002-6876-0802
Borhany, Munira
Department of Clinical Hematology, National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi – Pakistan
Shamsi, Tahir
Department of Clinical Hematology, National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi – Pakistan
term1,term7
Background: Immune thrombocytopenic purpura (ITP) is an autoimmune disorder characterized by low platelet count and various contributing factor. The imbalance in T cells may also cause ITP. Therefore, the present study was planned to assess the role of T-cells in pathogenesis of ITP and also to evaluate any possible link of Th:Tc imbalance to disease severity.
Methods: The present study was conducted with 111 patients of ITP and similar number of controls as case control study with 1:1 ratio of from January 2017 to July 2019. The patients were grouped according to the guideline of ASH as newly diagnosed/persistent ITP(ND-ITP/P-ITP) and chronic/refractory ITP(C-ITP/R-ITP).The blood samples were obtained, and CBC parameters were observed using advanced hematology analyzer XN-1000.The T cells subset analysis was evaluated by BD FACS Calibur flow cytometer. The Fisher exact test was done to evaluated the difference among the groups with (p=<0.05) by using SPSS version 19.
Results: Significantly reduced levels of hemoglobin, platelet counts with elevated IPF were observed in ND-ITP/P-ITP and C-ITP/R-ITP patients (p=<0.001).The significantly low Th:Tc ratio (p=<0.001) predicts imbalance of T cells in ND-ITP/P-ITP (0.86±0.47) as compared to control group (1.73±0.46).The mean of 0.84±0.34 Th:Tc ratio was observed in C-ITP/R-ITP children with ≤16 years. An insignificant difference (p= 0.89) was linked between children with non-severe chronic (0.84±0.42), severe chronic (0.82±0.49) and refractory ITP (0.85±0.51).
Conclusion: In ITP patients’ low levels of Th:Tc ratio was observed suggesting dysregulation of immune system. The chronicity of the disease may be linked to elevated production of Tc in children (≤16 years) with C-ITP/R-ITP.
Keywords: Immune thrombocytopenia; Platelets counts; Helper T-cells (Th); Cytotoxic T-cells (Tc)
The RunningLine
2023-04-14 10:29:04
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1654
Advancements in Life Sciences; Vol 10, No 1 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/447
2017-05-25T01:13:58Z
ALS:ART
nmb a2200000Iu 4500
"170525 2017 eng "
2310-5380
dc
Mitochondrial genetic characterization of Gujar population living in the Northwest areas of Pakistan
Ullah, Inam
Department of Genetics, Hazara University, Garden Campus, Mansehra 21300 Pakistan
Ahmad, Habib
drhahmad@gmail.com
Hemphill, Brian E
Department of Anthropology, University of Alaska, Fairbanks, Fairbanks, AK 99775, United States
Nadeem, Muhammad Shahid
Department of Biochemistry, Faculty of Science, King Abdulaziz University Jeddah 21589, Saudi Arabia
Tariq, Muhammad
Islamia College University, Peshawar, Khyber Pakhtunkhwa, 25120 Pakistan
Tabassum, Sadia
Department of Genetics, Hazara University, Garden Campus, Mansehra 21300 Pakistan
Genetics; Molecular Biology
Background: Diversity of communities with specific cultural, ethnic, lingual and geographical backgrounds makes Pakistani society a suitable study subject to unravel the early human migrations, evolutionary history of population having about 18 ethnic groups. Gujars are mostly Indic-speaking nomadic herders with the claims of multiple origins in the sub-continent. Present study was aimed at the determination of maternal lineage of Gujars by mitochondrial DNA analysis.
Methods: Total DNA from the human buccal cells was isolated using modified phenol chloroform method. Purified DNA was used for the PCR amplification of mitochondrial Hyper Variable Region 1 and 2 (HVR1 & 2). The nucleotide sequences of amplified PCR products were used to explore the maternal lineage of the Gujar population residing in Northern Pakistan.
Results: Haplotypes, allele frequencies and population data of the mitochondrial control region was determined in 73 unrelated individuals belonging to Gujar ethnic group of Northwest areas of Pakistan. Total 46 diverse haplotypes were identified out of which 29 were found unique with (0.9223) genetic diversity and (0.9097) power of discrimination. Haplogroup R was the most frequent (48%) followed by haplogroup M (45%) and N (7%).
Conclusion: We found that the Gujar population has multiple maternal gene pool comprising of South Asian, West Eurasian, East Eurasian, Southeast Asian and fractions of Eastern Asian, Eastern Europe and Northern Asian lineages. This study will contribute for the development of mitochondrial DNA database for Pakistani population.
The RunningLine
2017-05-25 01:13:58
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/447
Advancements in Life Sciences; Vol 4, No 3 (2017): Advancements in Life Sciences
eng
Copyright (c) 2017 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1271
2022-07-05T10:58:52Z
ALS:ART
nmb a2200000Iu 4500
"211231 2021 eng "
2310-5380
dc
Knowledge, attitude, and practices on human myiasis with spatial modeling of human risk of exposure to Oestrus ovis among shepherds/ people in Ilam province, southwest of Iran
Akbari, Morteza
Department of Medical Entomology & Vector Control, Tehran University of Medical Sciences, Tehran, Iran http://orcid.org/0000-0002-3224-3037
Akbarzadeh, Kamran
Rafinejad, Javad
Hanafi-Bojd, Ahmad Ali
Sheikhi, Soraya
Aivazi, Ali Ashraf
Background: Myiasis is the infestation of humans or other animals with the larvae of flies that occurs in the tropical and subtropical regions, worldwide. The objectives of the present study were to evaluate knowledge, attitude, and practice of the population at risk about myiasis and to infer potential risk areas of human myiasis due to the environmental suitability for Oestrus ovis in the Ilam province of Iran.
Methods: This study was conducted from April to June 2020 among the myiasis-infested shepherds in Ilam province. The data were collected by a questionnaire. Maximum Entropy (Maxent) niche modeling was used to predict the environmental suitability for O. ovis.
Results: The level of awareness of the people of Ilam province on myiasis was generally good, while a small percentage of them had poor information about this disease (1.62%). Mean Diurnal range and isothermality revealed the highest and lowest share on the MaxEnt model, respectively. The elevation variable had the most permutation in the model for predicting the environmental suitability for O. ovis. The best ecological niches for this fly were in the northern parts of the study area.
Conclusion: It can be concluded that due to background knowledge of the involved people in exposure to oral myiasis agent, O. ovis, conducting some preventive measures based on improving their knowledge, attitude, and practices could be useful for reducing the risk of the disease in the area. On the other hand, the suitable areas for the establishment of O. ovis which has been clarified with the model could be used for focusing on the preventive measures in the area.
Keywords: Modeling; Knowledge; Attitude; Practice; Human Myiasis; Iran
The RunningLine
2022-02-06 09:46:24
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1271
Advancements in Life Sciences; Vol 8, No 4 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1743
2023-11-02T15:34:59Z
ALS:ART
nmb a2200000Iu 4500
"231023 2023 eng "
2310-5380
dc
Effect of Serine on Growth and Biochemical Constituents of Zea mays L., Triticum aestivum L., and Abelmoschus esculentus L. under Arsenic Toxicity
Shahid, Sabrina
Department of Chemical and Life Science, Qurtuba University of Science & Information Technology
Peshawar, KP - Pakistan
Asad, Fayaz
Bacha Khan University Charsadda
Hussain, Fida
Department of Botany, Islamia College University Peshawar, KP - Pakistan
Yaseen, Tabassum
Department of Botany, Bacha Khan University Charsadda, KP - Pakistan
Dilawar, Naveen
Department of Botany, Abdul Wali Khan University Mardan, KP – Pakistan
Ahmad, Imtiaz
Department of Botany, Bacha Khan University Charsadda, KP - Pakistan
Vasila, Sharipova
Institute of Botany, Academy of Sciences Republic of Uzbekistan, Tashkend Durmon Yuli - Uzbekistan
Biochemistry; Plant Physiology
Background: Various human activities, such as industrialization, modern farming methods, and mining increase the concentration of heavy metals in air, water and soil. Heavy metal poisoning of soil results in a number of environmental issues and has deleterious effects on both plants and animals. Therefore, the purpose of this study was to investigate the effects of Arsenite (As) and As+ Serine (Ser) on growth and biochemical components in the early growth stages of Abelmoschus esculentus (L.) Moench, Triticum aestivum L., and Zea mays L. (selected crops).
Methods: Pot experiments were carried out at completely random manner, with 10-12 seeds grown in each pot with three replicates. Seeds and seedlings in pots treated with different concentrations of As and As+Ser. After a 21-days of germination period, we gathered the growth-related parameters (root number, root length, shoot length, and leaf number) and conducted a biochemical analysis.
Results: The growth of selected plants was adversely impacted by Arsenic stress, whereas the detrimental impact was minimal after treatments with Serine. Compression of the selected crops showed that Abelmoschus esculentus L. had the most detrimental impact on agronomic parameters. Biochemical constituents such Chlorophyll “a” “b”, Total-chlorophyll (Photosynthetic pigments), protein and carotenoid contents formation were reduced at individual treatments of As (25, 50, 75 and 100pmm) compared to As+Ser and control treatment, while the proline contents were increased considerably at treatment 100 ppm (As) of the selected crops.
Conclusion: The results showed that As had a greater negative impact on growth and biochemical constituents, whereas Ser had a reduced adverse impact on selected crops. Abelmoschus esculentusL. had a higher sensitivity compared to other selected crops.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1743
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/732
2019-05-26T05:27:07Z
ALS:ART
nmb a2200000Iu 4500
"190526 2019 eng "
2310-5380
dc
Evaluation of antimicrobial activity of a lichen used as a spice (Platismatia glauca)
Abdallah, Emad M.
Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia
Microbiology
Background: Lichen is a complex symbiotic relationship arose from algae or cyanobacteria that live together with some fungal species. Some of them are edible and consumed as spice such asPlatismatia glauca. The current study aimed to evaluate it’s the antimicrobial properties of the methanolic extract of lichen thalli of P. glauca against some referenced bacterial and yeast strains.
Methods: Disc diffusion test, minimum inhibitory (MIC) and minimum bactericidal (MBC) or minimum fungicidal (MFC) tests were carried out to evaluate the antimicrobial activity of lichen.
Results: All tested microorganisms exhibited varying degrees of susceptibility. Among the tested strains, the most susceptible bacterium -using the disc diffusion assay- was Staphylococcus saprophyticus (18.5±1.0 mm), followed by Staphylococcus aureus (14.5±0.5 mm), Shigella flexneri(12.5±1.5 mm), Streptococcus pneumoniae (12.0±1.0 mm), Proteus vulgaris (11.5±0.5 mm),Salmonella Typhimurium (11.5±0.5 mm), Bacillus cereus (11.0±1.0 mm) and Escherichia coli(11.0±0.0 mm), respectively. It also showed high antifungal activity against Candida albicans(22.5±0.5 mm). The MIC, MBC and MFC were promising, which were as low as 3.125 to 12.5 mg/ml for MIC and 6.25 to 12.5 mg/ml for MBC and MFC.
Conclusion: From the obtained results, it is concluded that the lichen thalli of Platismatia glaucapossesses a remarkable antimicrobial activity and it may be considered as a source of potential antimicrobial agents.
Keywords: Antibacterial, Antifungal, Lichen, Platismatia glauca
The RunningLine
2019-05-26 03:56:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/732
Advancements in Life Sciences; Vol 6, No 3 (2019): Advancements in Life Sciences
eng
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1065
2022-11-05T03:24:51Z
ALS:ART
nmb a2200000Iu 4500
"221104 2022 eng "
2310-5380
dc
Investigation of the effect of end season drought stress on morphological on Durum wheat genotypes
Khayatnezhad, Majid
Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
Gholamin, Roza
Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
Keynoos, Hasti
Department of Science, Cellular and Molecular Biology, Islamic Azad University, Ardabil - Iran
Background: Drought stress is one of the most important factors limiting the yield of crops, especially in semi-arid regions of the world. Therefore, studying and investigating plants resistant to these conditions can be useful for the agricultural industry and the country's economy.
Methods: To evaluate this capacity overall performance grain durum wheat genotypes in drought conditions and overview a number of the developments related to yield, and a few decided on advanced genotypes, 10 genotypes of Durum wheat within side the 2017-2018 cropping year.
Results: The evaluation of variance confirmed great variations among the developments evaluated in phrases of strain and there has been no tension. Also, amongst genotypes in phrases of height, important spike length, grain weight, and there has been a great distinction in yield. Performing evaluation Factor, via evaluation, most important four additives 82.67 percentage of overall running modifications have been justified.
Conclusion: The consequences imply the significance of component coefficients traits of overall and fertile tillers, main spike length, 1000-seed weight, and yield decided on genotypes is suitable for dry conditions.
The RunningLine
2022-11-04 09:11:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1065
Advancements in Life Sciences; Vol 9, No 3 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2077
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Isolation Toxoplasma gondii from Placental Tissue and its Effect on malondialdehyde levels in pregnant women in Al-Anbar Province
Jasim, Ridhab Ajeel
College of Pure Science, University of Anbar
Noori, Saif Subhi
College of Pure Science, University of Anbar
Mahmood, Adeeb Shakir
College of Pure Science, University of Anbar
Alkareem, Fatin Zuher Abd
College of Pure Science, University of Anbar
Background: Toxoplasmosis is recognized as among the prevalent ailments globally affecting mammalian creatures. Around a third of the Earth's inhabitants are afflicted with the condition. Miscarriage, also known as spontaneous abortion or pregnancy loss, pertains to an unviable intrauterine pregnancy that occurs within 20 weeks of gestation. Early pregnancy loss, which occurs throughout the first trimester, is the most common miscarriage type.
Methods: Alternative techniques that do not involve enzymes have been employed to separate the toxoplasma gondii parasite from placentas that have been aborted or not in women with toxoplasmosis in Al-Anbar Province. The purpose of such techniques is to preserve the parasite's viability while it rapidly multiplies inside placental tissue. Moreover, the current investigation measures the amounts of malondialdehyde (MDA) to determine how the parasite affects lipid peroxidation.
Results: Upon examining the pellet and suspension obtained from the placental solution, the microscopic analysis revealed the existence of bradyzoite in a majority of the identified specimens, along with the appearance of the swiftly reproducing tachyzoite phase in some. Lipid Peroxidation measurements showed that the infected samples' MAD levels had significantly increased relative to control samples.
Conclusion: Non-enzymatic methods of isolating the toxoplasma parasite are better at maintaining the vitality of the different phases of the parasite. Moreover, the parasite's effect is evident in raising lipid peroxidation levels and increasing risk factors.
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2077
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/889
2020-06-01T23:31:31Z
ALS:ART
nmb a2200000Iu 4500
"200601 2020 eng "
2310-5380
dc
Evaluation of Antibiotics Pattern of Extended Spectrum Beta-Lactamase Producing Multi-Drug Resistant Pseudomonas aeruginosa
Muhammad, Anees
Department of Medical Laboratory Technology, Medical Teaching Institutions, College of Medical Technology, Bacha Khan Medical College, Mardan, Pakistan
Ali, Ihsan
Department of Medical Laboratory Technology, Faculty of Basic & Applied Sciences, The University of Haripur, KPK, Pakistan
Owais, Muhammad
Department of Medical Laboratory Technology, Medical Teaching Institutions, College of Medical Technology, Bacha Khan Medical College, Mardan, Pakistan
Khan, Sadiq Noor
Department of Medical Laboratory Technology, Faculty of Basic & Applied Sciences, The University of Haripur, KPK, Pakistan
Afridi, Irfan Qadir
Khyber Teaching Hospital, Medical Teaching Institution, Peshawar-Pakistan
Ali, Nasir
Department of Medical Laboratory Technology, Medical Teaching Institutions, College of Medical Technology, Bacha Khan Medical College, Mardan, Pakistan http://orcid.org/0000-0001-5727-9573
Microbiology; Molecular Biology; Health and Epidemiological studies
Background: Pseudomonas aeruginosa (Ps. aeruginosa) is considered as an opportunistic pathogen and the leading cause of morbidity and mortality in immunocompromised individuals. Globally, approximately 10-15% of the nosocomial infections are caused by Ps. aeruginosa. The Ps. aeruginosa can acquire resistance against broad-spectrum antibiotics. According to recent studies increased mortality has been observed due to infection with extended-spectrum-beta-lactamase (ESBL) producing Ps. aeruginosa strains. This study was designed to determined antibiogram of ESBL producing multi-drug resistant Ps. aeruginosa in Khyber Pakhtunkhwa.
Methods: The clinical confirmed Ps. aeruginosa samples were collected according to the standard protocol, at Khyber Teaching Hospital (KTH), Peshawar. All collected samples were sub- cultured on appropriate culture media. After isolation and identification, the antibiotics susceptibility testing was performed. The detection of ESBL was carried out by the double-disc diffusion method. Carbapenemase-producing bacteria was confirmed by the modified Hodge test. Descriptive analysis was performed for statistical analysis of collected data.
Results: A total of one hundred and sixty-two (n=162) Ps. aeruginosa confirmed isolates were collected, in which 59.3% were male and 40.7% were from female patients. The percentages of ESBL and carbapenemase producing Ps. aeruginosa isolates were 5.5% and 23.5%, respectively. The multidrug resistance was observed against 27.2% isolates. Among tested antibiotics highest percentages of resistance was observed against ciprofloxacin (43%) and ceftazidime (39.5%).
Conclusion: We observed highest level of drug resistance in Ps. aeruginosa clinical isolates against tested antibiotics and majority of the isolates were Multi-drug resistant (MDR).
Keywords: Pseudomonas; Multi-Drug Resistant; Extended Spectrum Beta Lactamases; Antibiotics Susceptibility
The RunningLine
2020-06-01 03:39:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/889
Advancements in Life Sciences; Vol 7, No 3 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1200
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Synteny of Cotton SSR Markers Genomes Paves the Way for Resistance Against Black Root Rot Disease in Cotton
Ai, Trinh Ngoc
Tra Vinh University, No. 126 Nguyen Thien Thanh Street, Ward 5, Tra Vinh City, Viet Nam
Bui, Anh Phu Nam
Institute of Applied Technology, Thu Dau Mot University, Binh Duong province, Vietnam
Molecular Biology, Biotechnology
Background: Black root rot disease is documented for substantial reducing cotton yield and fiber quality. The isolation of candidate resistant genes in tetraploid genome AADD cotton species (2n=4x=52) remains challenging in the absence of research of black root rot resistance on progenitor DD genome diploid cotton. In this study, by exploiting Phytozome database, a comparative map of the black root rot-resistance quantitative trait loci in DD genome was constructed.
Methods: Simple sequence repeats markers associated with these three quantitative trait loci in the AA genome were used as “anchored-probes” frameworks for establishing relationships between the two cotton genomes AA and DD.
Results: Our findings showed that there was conserved orders among mapped simple sequence repeats markers on AA genome and the physical map of these simple sequence repeats markers on DD genome.
Conclusion: It was suggested that the syntenic loci on chromosome 2, 7 and 11 on DD genome could harbor the resistance gene against the black root rot disease. This study could serve as a fundamental step in isolating and introducing the resistance gene against black root rot into elite cotton cultivars.
Keywords: Comparative mapping; Resistance gene; Phytozome; Simple sequence repeats; Quantitative trait loci
The RunningLine
2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1200
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/972
2020-11-27T03:56:41Z
ALS:ART
nmb a2200000Iu 4500
"201127 2020 eng "
2310-5380
dc
Study on significant changes in calcium, phosphorus and thyroid hormones level in hypothyroidism patients
Zahra, Nida
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan
Ali, Ahmad
National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad
Kousar, Saima
Department of Psychology, University of Sargodha, Sargodha, Pakistan
Malik, Arif
Dr. Arif Malik
IMBB, University of Lahore, Lahore.
Email: arif.malik@imbb.uol.edu.pk
Zaheer, Ahmad
IMBB, University of Lahore, Lahore
Email: zaheer5500248@gmail.com
Malik, Imran Riaz
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Biochemistry
Background: Thyroid hormones have a vital role in metabolism of lipids, cholesterol etc. They also have an important role in phosphorous and calcium homeostasis by their direct effect on bone turnover. The objective of present study was to find the significant differences of T3, T4, TSH, lipid profile (HDL, LDL, vLDL, TG, Cholesterol), electrolytes and minerals (sodium, potassium, chloride, phosphorus, calcium) between control group and hypothyroidism patients.
Methods: Blood samples were collected from 72 patients and 12 control after their consent. Serum was used to determine biochemical parameters using standard protocol. Data obtained were statistically analyzed using “t” student test.
Results: The level of TSH was significantly higher in female and male hypothyroidism patients (p < 0.01) as compared to control and it was inversely related to the level of T3 and T4. Patients with hypothyroidism have increased level of LFTs profile especially cholesterol and Triglycerides levels both in males and females. In hypothyroidism, the level of glomerulus filtrate rate decrease especially sodium level but thyroid hormonal disturbance does not affect serum electrolyte level. The serum phosphorus and calcium levels change significantly in thyroid disorder.
Conclusion: Hypothyroidism is associated with increased TSH level and decreased T3 and T4 levels but does not appear to be associated with abnormalities in lipid profile. The serum phosphorus and calcium levels change significantly in thyroid disorder, Treatments of its primary causes should be given and if it is possible minerals can be added to avoid further bone complications.
Keywords: Hypothyroid; T3; T4, TSH; Lipid profile; Mineral
The RunningLine
2020-11-27 03:56:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/972
Advancements in Life Sciences; Vol 8, No 1 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1530
2023-04-14T10:29:04Z
ALS:ART
nmb a2200000Iu 4500
"230414 2023 eng "
2310-5380
dc
Revealing the Yield and Quality Responses of Soybean Advanced Lines under Semi-Arid Conditions
Altaf, Muhammad
Oilseeds Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Sarwar, Sehrish
Oilseeds Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Iqbal, Junaid
Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Faisalabad
Rahman, Sajid ur
Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Mustafa, Hafiz Saad Bin
Oilseeds Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Nazir, Shahid
Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Habib, Imran
Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Jhang Road, Faisalabad.
Nawaz, Muhammad
Lim Project Wapda, Hyderabad, Sindh.
oilseeds; soybean; yield; quality
Background: Soybean as human diet is a rich source of protein and oil. It also plays a vital role in livestock and poultry industries. Objective of this work is to exploit the local soybean germplasm for semi-arid conditions.
Methods: The experiment was conducted in Randomized Complete Block Design with three replications. Plant × plant and row × row distance was maintained as 4 inch and 1ft respectively. At maturity data for plant height, days to 50% flowering, no. of branches, no. of pods, grains per pod and grain yield per hectare were recorded. Furthermore, oil percentage, protein percentage, omega-3, omega-6, omega-9, palmitic acid and stearic acids were also measured.
Results: All genotypes showed highly significant difference from each other for selected traits. Grain yield per hectare was significant in genotypes such as CN-5, FS-10, E-402 and SH-1274 as compared to Faisal soybean (check). Protein and oil percentage were significantly more in CN-5, HS-17 and FS-10. Branches per plant significantly correlated with the yield but protein and oil percentage negatively correlated with each other. PCA indicated that only four out of 13 PCAs exhibited more than 1 Eigen value and showed 76.53 % variation. All traits for yield and quality were presented in PCA1, PCA2 and PCA3. Biplot indicated that genotype CN-5, SH-1274 and HB-17 falls in the positive portion that perform good.
Conclusion: Soybean genotypes CN-5 and FS-10 showed the more yield with high protein and oil percentage as compared to check variety and could be used in semi-arid environments.
Keywords: Oilseeds; Soybean; Semi-arid; Yield; Quality
The RunningLine
2023-04-14 10:29:04
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1530
Advancements in Life Sciences; Vol 10, No 1 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2385
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
Sphingomyelin, Plasminogen, and Docosahexaenoic in Sera of Autism Spectrum Disorder Children
Hamoud, Ali Fadheel
Ministry of Education, Karbala Education Directorate, Kerbala, Iraq
Al-Saadi, Narjis Hadi
University Specialization: biochemistry
Department of Chemistry- College of Science - Karbala University- Karbala - Iraq.
Background: Autism spectrum disorder (ASD), more commonly referred to as autism, is a neurodevelopmental disorder that is pervasive, highly heritable, and extremely variable. It is characterized by underlying cognitive features that frequently co-occur with other conditions. Since ASD is a multifactor disease, genetics, and environmental factors can play crucial roles in its progression. However, very few biological parameters can be used as a prediction for ASD which can help in diagnosis and starting the treatment early. Given the rapidly increasing prevalence of ASD, there is an urgent need to identify related diagnostic biomarkers. This study aims to investigate the association between some blood parameters that can be used to predict ASD and classify the severity, which were the main aims of the current inquiry.
Method: A case-control study was conducted on children with ASD, 37 Kids with ASD participated in the current investigation and 46 kids as the control group, their ages were between 3-12 years. Children with ASD were divided into two subgroups depending on the severity of ASD using the Gilliam scale. Competitive and sandwich ELISA were used to measure the biochemical markers of this study.
Result: After blood samples were collected three parameters were measured (sphingomyelin, plasminogen, and docosahexaenoic acid). In medium ASD cases, the results display that there is a significant increase in all parameters (sphingomyelin, plasminogen, and docosahexaenoic acid) respectively [(OR:4.691, CI:1.289~17.068, p=0.014), (OR:7.5, CI:1.844~30.509, p=0.001), (OR:5.156, CI:1.412~18.831, p=0.001)]. On other hand, in under medium cases of ASD, there is a significant decrease in Sphingomyelin levels (OR:0.97, CI:0.356~0.836, p=0.001), plasminogen (OR: 0.5, CI: 0.169~0.560, p=0.05), and docosahexaenoic (OR: 0.22, CI: 0.63~1.771, p=0.003) when compared with the control group.
Conclusion: In sum, our results showed that these noninvasive parameters can be used as biomarkers for ASD diagnosis and disease propagation. More research needs to be done to cover other pathophysiology parameters with genetics analysis for ASD that can be used as prediction biomarkers.
Keywords: Autism spectrum disorder; Sphingomyelin; Plasminogen; Docosahexaenoic acid
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2385
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/191
2016-08-25T01:00:24Z
ALS:ART
nmb a2200000Iu 4500
"160825 2016 eng "
2310-5380
dc
Detection of Endothelin 2 (Et-2) as a novel biomarker of renal dysfunction in patients of liver Cirrhosis with Ascites
Qureshi, Amna
a. Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000, Pakistan
Zaigham, Kalsoom
a. Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000, Pakistan
Iqbal, Muhammad Shahzad
b. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
Ali, Qurban
b. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
Health and Epidemiological studies
Background: Hepatorenal dysfunction in liver cirrhosis is a condition in which there is progressive kidney failure. It is a serious complication that can lead to death. Endothelin is an endothelial cell-derived peptide which is involved in renal dysfunctioning by generating oxidative stress in individuals suffering from liver cirrhosis. It can be used as important biomarker of hepatorenal dysfunction. The present study was aimed to detect endothelin-2 in the blood of patients of liver cirrhosis with ascites as important biomarker correlated to the generation of oxidative stress and renal dysfunctioning.
Methods: 50 blood and ascitic fluid samples were collected from the patients of liver cirrhosis with ascites as case group and 25 from normal individuals as control group. Endothelin-2 was detected by PCR in serum and ascetic fluid of patients and its quantification was done by Real-time PCR. ET-2 was quantified in serum samples but was not quantifiable in ascetic fluid might be due to its very low concentration. Blood urea, creatinine and uric acid with GFR were estimated as renal dysfunction parameters. Anti-oxidative enzymes glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MAD) as biomarkers of oxidative stress were estimated in order to calculate oxidative stress. The obtained data was evaluated statistically by using t-test and Pearson correlation.
Results: Positive correlation was found between individual parameters of oxidative stress and renal dysfunction. Overall positive and highly significant (P<0.05) correlation of CAT, SOD, MDA, GSH levels with each other was observed, a significant (P<0.05) and positive correlation of oxidative stress parameters were found with urea, creatinine and uric acid with Glomerular filtration rate (GFR).
Conclusions: It was concluded that the production of ET-2 under oxidative stress generates renal dysfunction in patients of liver cirrhosis with ascites.
The RunningLine
2016-08-25 01:00:24
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/191
Advancements in Life Sciences; Vol 3, No 4 (2016): Advancements in Life Sciences
eng
Copyright (c) 2016 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1037
2022-07-05T10:48:19Z
ALS:ART
nmb a2200000Iu 4500
"211231 2021 eng "
2310-5380
dc
Determinants of COVID-19 Anxiety in Pakistan Through Binary Logistic Regression
Mehmood, Qaisar
Government Post Graduate College Bahawalnagar, PhD Scholar at Department of Economics and Statistics, Dr Hasan Murad School of Management, University of Management and Technology Lahore
Sial, Maqbool Hussain
Department of Economics and Statistics, Dr Hasan Murad School of Management, University of Management and Technology Lahore
Sharif, Saira
Department of Economics and Statistics, Dr Hasan Murad School of Management, University of Management and Technology Lahore
Riaz, Muhammad
Department of Statistics Sub Campus Rahim Yar Khan Islamia University of Bahawalpur
Health and Epidemiological studies
Background: This study was conducted to evaluate the determinants of anxiety among the people living in hassled environment for last few months due to outbreak of COVID-19.
Methods: 279 participants were interviewed through a questionnaire about COVID-19 anxiety on four likert scales. Data was analyzed by using statistical software IBM SPSS Statistics 21. A binary logistic regression was used to estimate relationship between anxiety and T.V news, social media news, social isolation, disturbance of sleep and decrease in daily income. Significance of these factors are tested at 5 percent level of significance.
Results: 90.33 percent of the respondents are feeling COVID-19 anxiety while 9.67 percent peoples are taking it mild. The values of test statistics for the variables listening of T.V news, social media news, social isolation, disturbance of sleep and decrease in daily income are highly significant at p < 0.05.
Conclusion: The existence of anxiety was found to be highly significance due to listening of T.V news, social media news, social isolation, disturbance of sleep and decrease in daily income.
Keywords: COVID-19; Anxiety; T.V news; Social media news; Social isolation; Binary logistic regression
The RunningLine
2022-02-06 09:46:24
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1037
Advancements in Life Sciences; Vol 8, No 4 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1803
2023-07-15T04:31:43Z
ALS:ART
nmb a2200000Iu 4500
"230715 2023 eng "
2310-5380
dc
Prospects for the Therapeutic Potential of Inhibitors Targeting JNK and p53 in Alzheimer's Disease
Zyuz`kov, Gleb
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia http://orcid.org/0000-0003-0384-333X
Miroshnichenko, Larisa
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia
Chayikovskyi, Alexander
Kotlovskaya, Larisa
Alzheimer's disease; neural stem cells; β-amyloid; JNK; p53
Background: It is promising to search for fundamentally new approaches to the treatment of Alzheimer's disease (AD) within the framework of creating methods for stimulating neurogenesis and developing a strategy for targeted pharmacological regulation of intracellular signal transduction in regenerative-competent cells (RCCs). Of particular interest in this case are JNK and p53. The purpose of the work was to investigate the possibility of regulating the RCCs functions using JNK and p53 inhibitors in the modelling β-amyloid-induced neurodegeneration (βAIN) in vitro.
Methods: The studies were performed using C57BL/6 mice. To model βAIN in vitro, the 25-35 fragment Amyloid β(βA) was used. The effect of the JNK inhibitors (SP600125) and p53 (Pifithrin-a, Cyclic) on the functioning of different types of progenitors and glial cells of the subventricular zone of the cerebral hemispheres (SVZ)was studied. NPCs, astrocytes, oligodendrocytes, and microglial cells were isolated from SVZ cells using immunomagnetic separation.
Results: We found the ability of the JNK inhibitor to stimulate the proliferation of NSCs and NPCs in βAIN simulation. While the p53 inhibitor is characterized by stimulatory activity in relation to the proliferation of only committed neuronal precursors under conditions of neurotoxic exposure to βA. In addition, inhibition of JNK and p53 resulted in stimulation of the secretion of neurotrophins by oligodendrocytes and microglial cells in the in vitro βAIN model.
Conclusion: The results indicate the potential ability of the JNK inhibitor to most consistently stimulate the implementation of the proregenerative properties of progenitor and neuroglial cells in AD.
Keywords: Alzheimer's disease; Neural stem cells; β-amyloid; JNK, p53
The RunningLine
2023-07-15 04:31:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1803
Advancements in Life Sciences; Vol 10, No 2 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/657
2018-11-27T02:30:53Z
ALS:ART
nmb a2200000Iu 4500
"180825 2018 eng "
2310-5380
dc
Rapid commercial conversion of agriculture land in Lahore Division, Pakistan
Hassan, Imtiaz
CRP Department UET Lahore
CRP Department UET Lahore
Background: Pakistan is experiencing rapid population growth and urbanization which require land in urban areas. Rapid commercial conversion of has dramatically changed agriculture land base. The present conversion policy is not capable to achieve principles and objectives of sustainability especially in agriculture areas.
Methods: The methodology includes literature review and collection of data by using random systematic sampling technique. Surveys of key informants, adjoining neighbors and owners of properties were conducted in the field. The study is focused to identify the negative effects of commercial conversion of land uses; major causes of illegal conversion; major driving forces; actors behind the commercial conversion of agriculture land and profit maximization arising from increasing demand of commercial spaces, physical aging of residential building structure leading to obsolesces are the main contributing factors giving rise to commercial conversion of land uses in Lahore Division.
Results: The researcher founded that main problems of study area resulting from illegal land conversion include shortage of parking space, security risks, harassment of LDA staff, traffic congestion, environmental pollution, destruction of social life, lack of utility services, abuse of land and poor governance.
Conclusion: The commercial conversion is dimensioning agriculture land and development is taking place in piecemeal fashion. The study recommends the dire need of spatial planning, proper zoning, enforcement of value tax, sustainable commercial conversion policy, compact and mixed development policy, sustainable land use planning and development of a planning system with effective enforcement measures which itself guarantee a sustainable land use development and rational utilization of prime agriculture land.
Keywords: Commercial Conversion, Land Use, Master Planning, Local Government, Driving Forces, Driving Actors and Agriculture Zone
The RunningLine
2018-08-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/657
Advancements in Life Sciences; Vol 5, No 4 (2018): Advancements in Life Sciences
eng
Copyright (c) 2018 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1283
2022-08-27T03:36:01Z
ALS:ART
nmb a2200000Iu 4500
"220827 2022 eng "
2310-5380
dc
The effect of Syzygium aromaticum (clove) on inflammatory markers (total leukocyte count, differential leukocyte count and tumor necrosis factor-alpha)
Tabassum, Saima
Niazi Medical College
Sargodha, Pakistan
Anjum, Ambreen
Associate Professor
Department of Physiology
Al Aleem Medical College
Lahore, Pakistan
Manzoor, Sobia
Senior Registrar Plastic Surgery
Mayo Hospital Lahore
Pakistan
Anwar, Wardah
Associate Professor
Department of Physiology
Al Aleem Medical College
Lahore, Pakistan
Ghouri, Muhammad Hashim
Department of Critical Care
Kaul Associates Lahore https://scholar.google.com/citations?user=5PWvNWMAAAAJ&hl=en http://orcid.org/0000-0003-2079-9799
Inflammation; Physiology; Molecular Biology; Inflammatory Markers; Health studies
Background: Inflammation is involved in pathogenesis of many diseases. Antiinflammatory chemicals can be used to treat such illness,especially if they are derived from plant sources as they will have fewer side effects.
Objective:to find out the outcome of ethanolic extract of syzygium aromaticum flower buds on markers of inflammation in albino rats.
Material and method: Antiinflammatory activity was investigated in albino rats using a experimental model of inflammation, the “formalin test” (injecting 5 % formalin into subplantar surface of right hind paw). Ninety rats were uniformly divided into three groups designates as control A,experimental B and refrence C. Intraperitoneal injection of normal saline, syzygium aromaticum flower bud extract and indomethacin was used. In these rats, foot edema was measured by Vernier caliper. Blood sampling was done through cardiac punture under anaesthesia to determine total and differential leukocyte counts and serum tumor necrosis factor alpha (TNF-α) levels.
Results: The clove extract produced significant (p=0.00) decrease in inflammatory response initiated by 5% formalin. The extract significantly decreased (p=0.009) serum TNF-α. However, its effect on the total and differential leukocyte counts was non-significant (p˃0.05).
Conclusion: The ethanolic extract of syzygium aromaticum possesses potent anti-inflammatory effects.
The RunningLine
2022-08-27 03:36:01
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1283
Advancements in Life Sciences; Vol 9, No 2 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1993
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Effect of Vitamin D3 Levels on Varicella-Zoster Virus Infection and IFN-Gamma Expression in Children: A Cross-Sectional Study in Iraq
Yousif, Maitham G.
Biology Department, College of Science University of Al-Qadisiyah
Al-Amran, Fadhil G.
Liverpool John Moors University
Rawaf, Salman
Cardiovascular Department, College of Medicine, Kufa University
Al-Jumeily, Dhiya
Public Health Director, WHO Collaboration Center, Imperial College, London
Background: This study aimed to explore the correlation between Vitamin D3 levels and IFN-Gamma expression in children residing in the southern and central provinces of Iraq. Vitamin D3 plays a pivotal role in immune function, and IFN-Gamma is a crucial cytokine involved in antiviral defense. Investigating the connection between Vitamin D3 and IFN-Gamma offers valuable insights into immune responses and potential implications for infectious diseases.
Methods: A case-control study was conducted, involving children from various schools and kindergartens in the southern and central provinces of Iraq. The study assessed Vitamin D3 levels and measured IFN-Gamma expression. Statistical analyses were performed to determine the relationship between these variables.
Results: The outcomes revealed a significant positive correlation between Vitamin D3 levels and IFN-Gamma expression within the study population (p < 0.05). Children with higher Vitamin D3 levels exhibited elevated IFN-Gamma expression, suggesting a possible immunomodulatory impact of Vitamin D3 on IFN-Gamma production.
Conclusion:These findings underscore the importance of maintaining adequate Vitamin D3 levels to support immune function, particularly in relation to IFN-Gamma expression. Improving Vitamin D3 status could potentially bolster antiviral defense mechanisms and reduce susceptibility to viral infections among children in the southern and central provinces of Iraq. Further investigation is warranted to delve into the underlying mechanisms and potential clinical ramifications of this significant association. Additionally, exploring the long-term effects of maintaining optimal Vitamin D3 levels on immune function and the outcomes of infectious diseases in this population would offer valuable insights for preventive and therapeutic strategies.
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1993
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/876
2020-02-26T15:09:38Z
ALS:ART
nmb a2200000Iu 4500
"200226 2020 eng "
2310-5380
dc
Evaluation of isolated Lactobacillus strains as Probiotics in yogurt preparation
Rubbani, Umaima
M.Phil scholar, Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan
Iqbal, Atia
Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan
food microbiology
Background: Probiotics are live organisms that when ingested in adequate amount are believed to provide health benefits to the host. Probiotics are when combined with prebiotics are termed as synbiotics and in that case prebiotics enables the probiotic organisms to survive better in the host. The main objective of this study is to investigate Lactobacillus strains isolated from dairy products with significant probiotic potential and their utilization in yogurt preparation.
Methods: Eleven (11) bacterial strains were identified as Lactobacillus by following Bergey’s manual scheme. Antibacterial activity was checked by agar well diffusion method. Their ability to grow at different bile salt concentration, pH concentration and in the presence of pepsin enzyme was checked under in vitro condition. Finally, they potential strains were evaluated as probiotic starter in yogurt preparation.
Results: Out of total strains isolated, 52% strains were considered as Lactobacillus. Half of the strains (50%) showed antibacterial activity against selected pathogens and the best zone was formed by S4BM2 (15mm) against Salmonella typhi. All the isolated strains had the ability to grow in the presence of 0.2% bile salt concentration; at pH 3, pH5 and in the presence of pepsin enzyme. The efficacy of Lactobacilli strains as starter culture in yogurt was checked and found that yogurt processed with combination of S2Y2 with apple pieces showed significant results as compared to uninoculated yogurt.
Conclusion: It was concluded that strain i.e. S2Y2 can further be used in different dairy industries for yogurt processing to improve the quality of yogurt.
Keywords: Fermentation; Prebiotics; Probiotics; Synbiotics; Syneresis; Yogurt
The RunningLine
2020-02-26 15:09:38
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/876
Advancements in Life Sciences; Vol 7, No 2 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1528
2022-11-05T04:54:58Z
ALS:ART
nmb a2200000Iu 4500
"221104 2022 eng "
2310-5380
dc
Antiparasitic Activity of Chemically Synthesized Magnesium Oxide Nanoparticles against Small Ruminant Haemonchosis
Imran, Muhammad
University of Agriculture, Faisalabad, Pakistan http://www.uaf.edu.pk/EmployeeDetail.aspx?userid=932
Alsayeqh, Abdullah F
Veterinary Medicine, College of Agriculture and Veterinary Medicine, Wasim University, Buraidah, Saudi Arabia.
Parasitology; Helminthology; Veterinary Sciences; Veterinary Medicine
Background: Haemonchosis, a debilitating parasitic disease of small ruminants, is a major threat to food security globally. The development of alternative antiparasitic interventions is an important measure toward reducing the progress of veterinary drugs resistance in food animals. Hence, reducing the risks related to chemical foodborne hazards exposure. This study was designed to investigate the anti-parasitic activity of magnesium oxide nanoparticles (MgO-NPs) against different stages of Haemonchus contortus.
Methods: Preparation of magnesium oxide nanoparticles was conducted by sol-gel method at room temperature. For anti-parasitic activity of nanoparticles and commercially available anthelmintics, the adult worms and eggs (collected from gravid female worms) were subjected to adult motility assay and egg hatch assay, respectively. The relative efficacy of both nanoparticles and anthelmintics was classified by identifying the lethal dose LC50.
Results: Wormicidal effects of MgO-NPs were found to depend on both the concentration and the amount of time, they were exposed to. The lower concentrations of the nanoparticle (1 to 5 µg/mL) have not shown any mortality during the first four hours of the incubation. However, the higher concentrations of the nanoparticle (10, 15, 20, and 25 µg/mL) harmed the parasite during this time. The highest dose (25 µg/mL) was capable of killing almost all the worms during the first 16 hours. The highest concentration of nanoparticle induced 90±2.89% egg hatch inhibition and 91±3.3% adulticidal activity for egg hatch assay and adult motility assay, respectively.
Conclusion: Magnesium oxide nanoparticles have shown potent anti-parasitic properties. The study may provide potential alternative anthelmintic to control gastrointestinal parasites particularly haemonchosis in the scenario of emerging anthelmintic resistance.
The RunningLine
2022-11-04 09:11:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1528
Advancements in Life Sciences; Vol 9, No 3 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2255
2024-01-08T07:56:30Z
ALS:ART
nmb a2200000Iu 4500
"240108 2024 eng "
2310-5380
dc
Repeated Subcutaneous Granulocyte Colony Stimulating Factor Effect in Clinical Pregnancy Rate
Alfil, Aseel
Department of Clinical infertility and Reproduction/ High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/ AL Nahrain University. http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
Al Anbari, Lubna
Department of Clinical infertility and Reproduction/ High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/ AL Nahrain University. http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
Biochemistry, Health and Epidemiological studies
Background: One of the most important challenges to the mother during pregnancy is the implantation of a semi-allogenic fetus. Granulocyte Colony Stimulating Factor is a polypeptide glycoprotein mainly used in hematological diseases and post-chemotherapy, but it has recently gained some popularity as an immune-modulating agent in reproductive medicine. The study aimed to define the influence of repeated subcutaneous G-CSF administration in infertile females submits to intracytoplasmic sperm injection.
Method: Ninety patients were taken from an outpatient infertility clinic and divided into two groups on the day of embryo transfer. The control group (49 patients) received no extra intervention, while the G-CSF group (41 patients) received a subcutaneous injection of G-CSF the first hour after embryo transfer, followed by weekly injections until a positive fetal heartbeat was detected.
Result: In the G-CSF group, the implantation rate was 20.4% compared to 7.6% in the control. In the control group, the rates of both chemical and clinical pregnancy were 18.4%, whereas they were 48.8% and 43.9%, respectively, in the G-CSF group.
Conclusion: The multiple doses of G-CSF significantly improved the implantation and pregnancy rates, so it is considered a promising drug in reproductive medicine.
The RunningLine
2024-01-08 07:56:30
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2255
Advancements in Life Sciences; Vol 10, No 4 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/68
2014-08-29T00:38:17Z
ALS:ART
nmb a2200000Iu 4500
"140825 2014 eng "
2310-5380
dc
Analysis of growth response and tolerance index of Glycine max (L.) Merr. under hexavalent chromium stress
Amin, Hira
Institute of Plant Sciences, University of Sindh, Jamshoro - 76080, Pakistan
Arain, Basir Ahmed
Institute of Plant Sciences, University of Sindh, Jamshoro - 76080, Pakistan
Amin, Farah
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro -76080, Pakistan
Surhio, Muhammad Ali
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro -76080, Pakistan
Environmental Biotechnology: Plant sciences
Background: Metal pollution has become one of the most serious environmental problems due to various human activities. It can damage or alter the biosphere reducing the agricultural productivity and can affect both animals and humans. Emission of various pollutants into the atmosphere has many harmful effects on plant growth. Rapid urbanization, unregulated industrialization, growing transport, metal plating and agricultural activities have created a problem of heavy metals contamination.
Methods: A greenhouse experiment was conducted to determine the toxicity of chromium onGlycine max. Chromium concentration applied to G. max was managed as 0.5, 2.5, 5, 10, 25, 50 and 100 mg kg-1 for experimental period of 90 days. The phytotoxic effect of chromium metal was analyzed by studying seed germination, seedling vigor index, root and shoot length, root and shoot fresh and dry weights, chlorophyll content tolerance index.
Results: The data presented in this study showed that chromium metal adversely affects the seedling vigor of G. max and significantly (p<0.05) reduces seed germination and growth. The toxic effect of chromium on the seeds increased with increasing the concentration of the metal. It was also found that high concentrations of chromium (50 and 100 mg kg-1) can completely inhibit the seed germination.
Conclusion: The chromium metal is extremely toxic for seeds and young seedling of G. max at high concentrations. Moreover, G. max has little potential to counteract the deleterious effect of chromium metal in soil at aforementioned treatments. The results of the present study may help in better understanding of the mechanisms involved in pytoextraction.
The RunningLine
2014-08-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/68
Advancements in Life Sciences; Vol 1, No 4 (2014): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/966
2020-11-27T03:56:41Z
ALS:ART
nmb a2200000Iu 4500
"201127 2020 eng "
2310-5380
dc
Decreased Expression of Alpha Smooth Muscle Actin and Desmin Contributes to the Protection of Vitamin D3 against Diclofenac Induced Nephrotoxicity in Rats
Youssef, Sahar
Anatomy Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt http://orcid.org/0000-0002-6134-8348
Salah, Marwa
Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt http://orcid.org/0000-0001-8836-3612
Background: Diclofenac is widely prescribed for its analgesic and anti-inflammatory actions but it also has some harmful effects on the kidney. The current study was conducted to elucidate the possible mechanism of action of diclofenac sodium on kidney, and if it is affected by the addition of vitamin D.
Methods: Rats were divided into 4 equal groups. G1 was the control group that received no treatment; G2 was treated with intramuscular injection of vitamin D (1,000 IU/kg, 3days/week); G3 was treated with intramuscular injection of diclofenac sodium (3.6 mg/kg, 3 days/week) and G4 treated simultaneously with both diclofenac (3.6 mg/kg, 3 days/week) and vitamin D (1,000 IU/kg, 3days/week) intramuscularly for four weeks. Kidneys sections were stained with H&E, Masson’s trichrome and immunohistochemical staining against α –SMA and desmin followed by the morphometric and statistical analysis.
Results: Kidney sections from diclofenac sodium treated group showed degeneration and necrosis, small or atrophic glomeruli with dilated Bowman’s space and some of the renal tubular lining cells appeared vacuolated with small pyknotic nuclei. Renal fibrosis was confirmed by significant increase in collagen fibers, α –SMA and podocytes injury by significant increase of desmin. However, in diclofenac- vitamin D treated group significantly the expression of α –SMA and desmin were decreased.
Conclusion: The current data suggested that vitamin D might play a protectant role against diclofenac induced kidney injury in rats through the preservation of the histological architecture of renal corpuscles, renal cortical tubules and down regulation of collagen, α – SMA and desmin.
Keywords: α-SMA; Desmin; Diclofenac Sodium; Vitamin D
The RunningLine
2020-11-27 03:56:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/966
Advancements in Life Sciences; Vol 8, No 1 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1550
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Animal Blood supplemented diet can improve growth performance, body composition and blood profile of Genetically Improved Farm Tilapia (Oreochromis niloticus)
Ambreen, Hafiza Samra
Institute of zoology, University of the Punjab, Lahore.
Arshad, Najma
Institute of Molecular Biology and Biotechnology, Centre for Research in Molecular Medicine (IMBB/CRIMM), The University of Lahore, 54792, Pakistan
Shahzad, Muhammad Mudassar
Government College University Faisalabad, University of Education
Javed, Ghulam Ayesha
Institute of zoology, University of the Punjab, Lahore.
Shazadi, Kiran
Institute of zoology, University of the Punjab, Lahore.
Ahmad Chaudhury, Farman
School of Food Sciences
And Technology
Minhaj University Lahore
Veterinary sciences
Background: Artificial feeding is an effective way to enhance fish production, development and carrying capacity of the culture system to feed the increasing human population. This study was designed to determine and compare the effects of supplementation of basal fish feed with plant (soybean meal) and animal blood as protein sources.
Methods: The experiment was conducted using a completely randomized block design. A total of 135 Genetically Improved Farm Tilapia were randomly divided into three groups comprising three replicates and kept in controlled conditions in nine glass aquaria for a period of ninety days. The animals were provided basal diet, plant and animal protein supplemented diets throughout the experiment. Water quality parameters were recorded on a routine basis while growth performance, blood indices and chemical analysis of the body was recorded after ninety days of trial.
Results: Overall, water quality parameters remained within the normal range, which highlights those diets had no detrimental effect on the quality of the water and in all groups. However, the fish kept on animal-based protein source diet presented higher growth performance, crude protein and lipids contents, red blood cell count and normal serum ALT, AST, and ALP levels In contrast the fish kept on plant protein diet displayed comparatively lower meat quality and signs of toxicity viz., raised level of hepatic enzymes.
Conclusion: From these results, it was concluded that fish fed on diet having blood meal supplementation showed higher performance in comparison to fish groups fed on other diets.
Keywords: GIFT; Fish feed; Plant meal; Soybean meal; Animal blood
The RunningLine
2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1550
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2717
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
The Therapeutic Effect of Hydroxychloroquine on Patients with Recurrent Miscarriage and the Risk Assessment of its Ocular Retinal Toxicity
Zhang, Ai
Department of Ophthalmology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, China
Zheng, Xianhe
Department of Ophthalmology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, China
Zhu, Xiaoli
Department of Gynecology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, China
Zheng, Jing
Department of Ophthalmology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, China
Health and Epidemiological Studies
Background: Recurrent miscarriage (RM) refers to the unfortunate loss of pregnancy at least three times before the 20th week of gestation. The present study examined hydroxychloroquine (HCQ)'s therapeutic effects in recurrent miscarriage owing to potential positive outcomes like increased rate of live births and negative outcomes like ocular and retinal toxicity.
Methods: The investigation involved the randomization of 400 mg of HCQ intervention in 91 pregnant women attending a monthly clinic at the Wenzhou TCM Hospital of Zhejiang Chinese Medical University.
Results: According to the results, HCQ results in positive pregnancy outcomes, with improving rates of live births with continued use of the medication. At the same time, the results produced significant safety issues, including HCQ deposition in the retina and ocular toxicity. An investigation of HCQ's effects on the retinal nerve fiber layer measurements, ganglion cell layer measurements, and the inner plexiform layer measurements revealed a dynamic trend.
Conclusion: We did not obtain conclusive outcomes regarding HCQ's effects on the changes in retinal measurements. However, the deposition of HCQ in the retina and the resulting vision loss, especially loss of color vision, stood out. These outcomes represent the safety and therapeutic effects of HCQ in pregnancy, posing concerns and creating the need for further studies. Interestingly, none of these outcomes were related to or dependent on the participants' age.
Keywords: HCQ; Recurrent miscarriage; Ocular retinal toxicity; Effects; Live births; Still birth
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2717
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/157
2016-11-24T01:23:28Z
ALS:ART
nmb a2200000Iu 4500
"151125 2015 eng "
2310-5380
dc
Effects of chronic mild stress on apomorphine induced behavioral sensitization in different brain regions of rats in relation to serotonin change
Farhan, Muhammad
Department of Biochemistry
University of Karachi
Haleem, Darakshan Jabeen
Biochemistry, Neuropharmacology, Neurochemistry
Background: The impacts of unpredictable stressors have influence on neurochemical and behavioral parameters in laboratory animals. Stress induced behavioral changes particularly those associated with anxiety like behavior may activate topographically organized mesolimbic cortical serotonergic system. This study was designed to investigate the influence of unpredictable stress on behavioral and neurochemical parameters in apomorphine treated rats.
Methods: Initially, the animals were divided into two groups as Unstressed and stressed (uncontrollable chronic mild stress or UCMS). Both groups of animals were subdivided into two groups; i.e. saline and apomorphine administrated animals at dose 1.0 mg/kg. Behavioral manipulations was observed by monitoring the locomotor activity and exploratory activity. Neurochemical estimation of 5-hydroxytryptamine (5-HT) was done by High performance liquid chromatography (HPLC). Animals were decapitated 24hr post apomorphine injection and different regions of brain (dorsal and ventral striatum), of animals were collected and stored at -70°C.
Results: This preclinical study showed that the UCMS induced hypophagia were promoted in apomorphine administrated animals. Apomorphine induced hyperlocomotion were more prominent in unstressed animals than that of stressed groups. It implies that apomorphine is effective in the retrieval from UCMS induced depressive symptoms in rats. Neurochemical study showed decreased level of 5-HT in unstressed animals than stressed animals in response to apomorphine administration.
Conclusion: This study, therefore establish the relation between stress and addiction at behavioral as well as neurochemical level to better understand the idea whether intolerable stress promotes addiction.
The RunningLine
2015-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/157
Advancements in Life Sciences; Vol 3, No 1 (2015): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/1057
2021-08-04T10:20:57Z
ALS:ART
nmb a2200000Iu 4500
"210804 2021 eng "
2310-5380
dc
Biofilm Forming Bacteria Isolated from Medical Implants
Fatima, Aimen
Kazmi, Mahwish Batool
Yasmeen, Humaira
Microbiology; Health and Epidemiological studies
Background: Biofilm formation in indwelling medical devices poses serious risk of infection and increases the likelihood of recurrence of infections. The study was carried out to identify the microbes which form biofilms on medical implants and are thus involved in nosocomial infections, to assess the potential of biofilm producing ability of these isolated microbes and to determine antibiotic resistance towards ampicillin, vancomycin ceftazidime, streptomycin and tetracycline.
Methods: For this, 11 samples of 5 different implants were taken from Tertiary Care Hospital Multan, Pakistan. Bacteria were isolated and identified by culture plate method. Tryptone soy broth (TBS) media was used for biofilm development by microbes in plastic tubes. Developed biofilm in tubes was visualized with crystal violet staining method and then biofilm forming potential was estimated by measuring the optical density through spectrophotometer. Antibiotic susceptibility was done by Kirby Bauer disk diffusion method to determine the resistance and susceptibility pattern of biofilm producers.
Results: Out of 11 different samples of indwelling medical devices, a total of 131 bacterial strains were isolated. The percentage of bacterial isolates which produced biofilms were Staphylococcus spp. (41%) followed by Escherichia coli (18%), Pseudomonas spp. (4%), Proteus spp. (7.2%), Klebsiella spp. (8.6%), Bacillus spp. (8.6%), Fusobacterium spp. (1.4%) Clostridium spp.(1.4%), Enterococcus spp. (7.2%) and Neisseria spp. (1.4%). Sixty-nine isolates were considered positive for biofilm formation while 58 were considered negative. The resistance was maximum against ampicillin (42%) followed by ceftazidime (17.1%), tetracycline (34%) and streptomycin (30%) while against vancomycin no resistance was observed.
Conclusion: Biofilms produced on medical implants by different bacteria are considered to be the major cause of hospital acquired infections and are very difficult to eradicate. These biofilms associated implant infections are challenging to treat because of their resistance towards various antibiotic therapies. Therefore, some efficient methods of prevention of biofilm formation should be introduced.
Keywords: Bacterial biofilms; Biofilm associated infections; Medical implants; Hospital acquired infections; Antibiotic resistance
The RunningLine
2021-08-04 10:20:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1057
Advancements in Life Sciences; Vol 8, No 3 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1681
2023-07-15T04:31:43Z
ALS:ART
nmb a2200000Iu 4500
"230715 2023 eng "
2310-5380
dc
Plastic Biodegradation Potential of Soil Mangrove Mold Isolated from Wonorejo, Indonesia
Kuswytasari, Nengah Dwianita
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Kurniawati, Alfia Rahma
Departement of Biology Education, Raden Fatah Islamic State University, Palembang, Indonesia
Aunurohim, Aunurohim
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Alami, Nur Hidayatul
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Zulaika, Enny
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Shovitri, Maya
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Kumari, Nimerta
Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
Luqman, Arif
Biology Department
Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Microbiology; Plastic degradation; Biodegradation; Fungi
Background: Plastic has become one of the most significant environmental threats, especially in mangrove areas due to their non-biodegradable nature. This indicates that there is a need to find alternative degradation methods for these materials, such as the use of mold. Therefore, this study aims to isolate potential plastic degradation mold from the Wonorejo mangrove sediments.
Methods: The isolates were identified based on their morphological characteristic and ITS rDNA sequence. Plastic degradation ability of the samples was evaluated using parameters of plastic dry weight loss, FTIR pattern, SEM, and AFM image.
Results: A total of eight sediment mold were identified, including Perenniporia spp., Trametes polyzona, Aspergillus terreus, Porostereum spadiceum, Leptosphaerulina chartarum, Aspergillus fumigatus, and Hypocreales sp. Among these molds, the best activity was exhibited by Aspergillus terreus, which degraded 12.5% LDPE and 4.9% white plastic after 30 days, followed by Perenniporia sp. with 10.6% LDPE and 3.4% white plastic degradation. Furthermore, the SEM and AFM images showed that the surface of plastic was damaged after incubation. There were also several attenuations of particular peaks, which indicated the occurrence of chemical changes along plastic chains belonging to the C-H alkane group at wavenumber 2914.94, 2847.38, and 1471 cm-1. Enzyme produced by mold in this study were measured qualitatively. Perenniporia sp. produced laccase, manganese peroxidase, alkane hydroxylase, and lipase. Trametes polyzona also secreted similar enzyme, except lipase, while Porostereum spadiceum was negative for alkane hydroxylase. Aspergillus terreus isolates were positive for lipase and alkane hydroxylase, but Aspergillus fumigatus only produced lipase.
Conclusion: In this study, 8 mold with plastic biodegradation potential by producing various enzyme were identified.
Keywords: Biodegradation, Enzyme, Mangrove; Mold; Plastic; Responsible consumption & production
The RunningLine
2023-07-15 04:31:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1681
Advancements in Life Sciences; Vol 10, No 2 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/573
2018-07-08T06:31:17Z
ALS:ART
nmb a2200000Iu 4500
"180525 2018 eng "
2310-5380
dc
Assessments of kidney function and morphology of tramadol-diclofenac treated albino rats
Adikwu, Elias
Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State
Nelson, Ebinyo C.
pharmacy/pharmacology
Background: Tramadol-diclofenac (TD-DF) could be used in chronic pain management. Concurrent use may present renal complications due to their individual nephrotoxic profile. The present study assessed the kidney function and histology of tramadol-diclofenac treated albino rats.
Methods: Forty two adult albino rats divided into seven groups A-G were used for this study. Rats were orally administered with TD (12 mg/kg/day), DF (6 mg/kg/day), and TD-DF for 14 days including two recovery groups. Rats were weighed and sacrificed at the termination of drug treatment. Serum was extracted from blood and evaluated for creatinine (Cr), urea (U), uric acid (UA), total protein (TP), albumin (Ab) and serum electrolytes (K+, Na+, Cl-, and HCO3-). Kidneys were excised weighed and evaluated for alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), superoxide dismutase (SOD), catalase (CAT) glutathione (GSH), glutathione peroxidase (GPX), malondialdehyde (MDA) levels and histological damage.
Results: The body weight, absolute and relative kidney weights and serum electrolytes were not significantly (p> 0.05) altered in the TD-DF treated rats in comparison to control. However, the levels of Cr, U, UA, AST, ALT, ALP and MDA were significantly (p<0.05) increased whereas Ab, TP, SOD, GSH, GPX and CAT were significantly (p<0.05) decreased in the TD-DF treated rats in comparison to treatments with individual doses of TD and DF. Varying degrees of histological damage were observed in the kidneys of TD-DF treated rats. However, nephrotoxic effects due to treatment with TD-DF were reversed in the recovery groups.
Conclusion: The use of tramadol-diclofenac could be associated with reversible nephrotoxicity; therefore renal function assessment is advised before tramadol-diclofenac use.
The RunningLine
2018-05-25 07:10:10
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/573
Advancements in Life Sciences; Vol 5, No 3 (2018): Advancements in Life Sciences
eng
Copyright (c) 2018 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1279
2022-05-29T08:45:07Z
ALS:ART
nmb a2200000Iu 4500
"220529 2022 eng "
2310-5380
dc
Sibling and non-sibling fingerprints comparison of Pakhtun population of Swat district, KP, Pakistan
Uddin, Subhan
Student
Centre for Animal Sciences and Fisheries, University of Swat http://orcid.org/0000-0003-0310-4596
Khan, Noor Ullah
Student
Centre for Animal Sciences and Fisheries, University of Swat
Rahat, Murad Ali
Ph.D
Department of Biotechnology and Genetic Engineering, Hazara University Mansehra
Ahmad, Aftab
Student
Centre for Biotechnology and Microbiology, University of Swat.
Akbar, Fazal
Associate Professor
Centre for Biotechnology and Microbiology, University of Swat.
Ullah, Naseer
Lecturer
Centre for Animal Sciences and Fisheries, University of Swat.
Shah, Muzafar
Assistant Professor
Centre for Animal Sciences and Fisheries, University of Swat.
Rasool, Akhtar
Assistant Professor
Centre for Animal Sciences and Fisheries, University of Swat.
Israr, Muhammad
Assistant Professor
Department of Forensic Sciences, University of Swat, Swat.
Background: Fingerprint and other ridges are considered to be the best forensic science tool for identification of humans, alive or dead, and even for decomposed bodies. These fingerprint ridges exhibit various static features throughout life which reflect the person biology. This branch gained immense importance since the past few decades in congenital abnormality. This study was to carry out fingerprints analysis of sibling and non-sibling for differentiation and gender identification.
Methods: A total of 80 pairs of fingerprints (1600 prints) were collected from persons aged 15 to 30 years using rolling method. Out of which 20 pairs were brother-brother, 20 were sister-sister, 20 were brother-sister and 20 Pairs were random. Each fingerprint was analyzed for the gender identification on the basis of minutiae, ridge density and types. All the fingerprints were analysed using ACE-V method. After comparison SPSS software was used for further analysis.
Results: Our result showed that the types of fingerprints identified was whorl (50%) followed by loop (45.25%), arch (4.5%) and 0.25% of the accidental type. The dominant type was whorl while accidental was the least common type of fingerprints. Statistical analysis showed that between the groups, brother-brother and sister-sister was significant while rest of the groups was not significant. Moreover, greater ridge density was observed in female as compared to male.
Conclusion: It is concluded that the sibling fingerprints had greater similarity as compared to non-sibling, however both male and female fingerprints were significantly different in term of ridges density. This study may be useful in crime scene investigation.
Keywords: Forensics; Fingerprint ridge density; Fingerprint type; Gender identification
The RunningLine
2022-05-29 08:45:07
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1279
Advancements in Life Sciences; Vol 9, No 1 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1973
2023-11-02T15:34:59Z
ALS:ART
nmb a2200000Iu 4500
"230930 2023 eng "
2310-5380
dc
Identification of phytochemicals as potential inhibitors against E6 protein of High-Risk Human Papillomavirus 16(HPV 16) via In-Silico Structure-Based Virtual Screening Approach
Jamal, Arshad
Background: The human papillomavirus (HPV) is a potentially fatal infection and the most common cause of cancer related feminine mortality around the world, thus requiring the design of anticancer drugs. The E6 oncoprotein is one of the most investigated therapeutic targets for cancer treatment. E6 oncoprotein plays a major role in tumor progression and cell immortalization. The E6 protein leads to the degradation of tumor suppressor protein P53 via interacting with E6 binding protein E6AP. Therefore, inhibiting the E6 protein can be a potential target for HPV.
Methods: In this study we performed virtual screening of 2296 phytochemicals library from MPD3 database against E6 protein.
Results: Three compounds were picked out as potential inhibitors. These compounds were selected considering their binding energy and hydrogen bond interactions. Further to verify the stability of the docked complexes 100ns molecular dynamics simulations were carried out.
Conclusion: Keeping in view the numerous analyses, we suggest that the potential three compounds could prove relevancy regarding the anti-HPV therapeutic advancements.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1973
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/765
2019-11-27T10:05:03Z
ALS:ART
nmb a2200000Iu 4500
"191127 2019 eng "
2310-5380
dc
Analysis of mRNA Expression for Injury-Age Estimation
Saba, Nusrat
Department of Forensic Sciences, University of Health Sciences, Lahore, Pakistan
Noreen, Saadia
Department of Forensic Sciences, University of Health Sciences, Lahore, Pakistan http://dnageography.com/team.php
Anjum, Mubarak Ali
Institute of Agricultural Sciences, Punjab University, Lahore, Pakistan
Ali, Saqib
Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan
Jawad, Muhammad
Rakha, Allah
Department of Forensic Sciences, University of Health Sciences, Lahore, Pakistan http://dnageography.com/team.php
Molecular Biology, Forensic Science, Forensic Genetics
Background: Determination of wound age is pivotal in forensic medical sciences, criminal and civil cases for the construction of crime scene and answering the questions like, time of infliction, manner of wound infliction, how long the person survives after infliction of wound and characterizing antemortem or postmortem wounds. The findings differ considerably among individuals due to the biological variations. Previous developed method in the injury-age determination is clinical, microscopic, enzymatic reaction at the wound margins, histological, and immunohistochemistry with the pitfalls associated with it.
Methods: This study is conducted on blunt injuries, particularly lacerated wound (type of wound inflicted by blunt weapon) to analyze the different expression pattern in injury-age up to 72hrs in total 21 individuals randomly grouped in different time intervals. To determine the time of injury, transcript abundance of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF was analyzed by real time polymerase chain reaction. 18S-rRNA was used as control marker.
Results: Percent knockdown (%KD) was calculated to determine the expression of mRNA for the determination of injury-age. IL1β and GM-CSF showed the predictive behavior for wound age up to 36hrs, Fibronectin (FBN) showed predictive behavior up to 12hrs while VEGFA showed prediction beyond 72hrs.
Conclusion: The detection of gradual decrease of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF may provide an estimation of wound-age.
Keywords: mRNA Expression; Injury-Age Estimation; Antemortem wounds; Postmortem wounds
The RunningLine
2019-11-27 09:56:35
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/765
Advancements in Life Sciences; Vol 7, No 1 (2019): Advancements in Life Sciences
eng
https://submission.als-journal.com/index.php/ALS/article/download/765/2734
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2895
2024-01-08T07:56:30Z
ALS:ART
nmb a2200000Iu 4500
"240108 2024 eng "
2310-5380
dc
Ameliorative effects of Vitamin E and Urtica dioica against thiamethoxam-induced teratogenicity in embryonated chicken eggs
Raza, Rameen
Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad.
Ahmad, Latif
Department of Pathology, Baqai Medical University (Veterinary Campus), Karachi
Saleemi, Muhammad Kashif
Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad.
Arshad, Muhammad Imran
Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan
Khatoon, Aisha
Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad.
Soomro, Hidayatullah
4Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand,
Khan, Ahrar
5Shondong Vocational Animal Science and Veterinary College, Weifang
Gul, Shafia Tehseen
University of Agriculture, Faisalabad
Veterinary Sciences
Background: The purpose of this study was to explore the defensive effects of Vitamin E (Vit. E) and Urtica dioica (UD) in the mitigation of developmental anomalies induced by thiamethoxam (TMX) in chicken embryos.
Methods: For this purpose, a total of 140 fertile eggs were equally divided into seven experimental groups (A-G); Groups A and B were kept as negative and sham control, respectively. Group C was exposed to TMX while groups D and E were supplemented with Vit. E and U. dioica, respectively along with TMX. Group F received Vit. E and group G U. dioica, only. The eggs were examined on days 10th and 20th of incubation for the assessment of developmental flaws and musculoskeletal anomalies.
Results: The mortality rate was highest (40%) in group C (TMX) followed by groups D and E (20 and 15%), respectively. Developing embryos were exposed to TMX for retarded growth weight and crown-rump length (CRL) were significantly decreased as compared to the control group. The highest survival rate was recorded in negative control group A. The teratogenic defects recorded in this study include growth retardation, decreased crown-rump length, shortened beak, exencephaly, feather scantiness, and limb deformities. Morphometric analysis revealed improved growth by all parameters in Vit. E and U. dioica supplemented groups.
Conclusion: It was concluded that developmental defects are due to induced TMX, can be counteracted with Vit. E and U. dioica and have no phytochemicals negative effects.
The RunningLine
2024-01-08 07:56:30
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2895
Advancements in Life Sciences; Vol 10, No 4 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/56
2014-06-05T02:51:20Z
ALS:ART
nmb a2200000Iu 4500
"140525 2014 eng "
2310-5380
dc
Biochemical basis of resistance in rice against Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae
Khan Khattak, Junaid Ahmad
PIEAS, ISLAMABAD
Afroz, Sehar
GC UNIVERSITY FAISALABAD
Arshad, Hafiz Muhammad Imran
NIAB
Sarwar, Nighat
NIAB
Anwar, Hafiza Sidra
NIAB
Saleem, Kamran
PLANT PROTECTION DIVISION, NUCLEAR INSTITUTE FOR AGRICULTURE AND BIOLOGY (NIAB) P.O.BOX-128, JHANG ROAF FAISALABAD
Babar, Muhammad Masood
NIAB
Jamil, Farhat Fatima
NIAB
AGRICULTURE, PLANT PATHOLOGY
Background: Bacterial Leaf Blight (BLB) is the most devastating disease of rice. This disease can reduce grain production by 20-50% and yield by 25%. The disease is widespread in Asia, United State, Latin America and Australia. In Pakistan, it is reported from all rice growing areas and it is increasing its area year by year. All famous Pakistan’s Basmati varieties are susceptible to BLB disease. The most economical way of controlling disease is to produce resistant varieties. Many different compounds are involved in resistance mechanism in host including phenolic compounds.
Methods: Plants inoculated with Xanthomonas oryzae pv. oryzae isolates i.e. 78.3 and 2.2 caused disease on all varieties were selected for investigation of phenolic compound. Randomized complete block design was used in field for inoculation and leaf collections for phenolic compounds determination. Data was phenolic compounds were analyzed through factorial ANOVA.
Results: The response of four varieties was different when inoculated with BLB isolate 2.2. Basmati 2000 variety of rice produced maximum amount of phenolic compounds after one week of inoculation of bacterial isolate 2.2 while the minimum total phenols were found in basmati 385 at 0 hrs. of inoculation (before inoculation). Xoo isolate 78.3 produced maximum phenolic compounds after 2 weeks in Basmati 2000.
Conclusions: Biochemical resistance due to high phenolic contents in Basmati-385 and Basmati-2000 is suggested. High phenol production may be due to loss of virulence in bacterial isolates. Both 78.3 and 2.2 Xoo isolates are significantly different in producing total phenols in all the varieties tested.
The RunningLine
2014-05-25 00:00:00
Peer-reviewed Article
https://submission.als-journal.com/index.php/ALS/article/view/56
Advancements in Life Sciences; Vol 1, No 3 (2014): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/2022
2024-03-06T16:36:17Z
ALS:ART
nmb a2200000Iu 4500
"240306 2024 eng "
2310-5380
dc
Effect of soybean milk dregs fermented with Aspergillus ficuum in rations on the performance and quality of quail eggs
Ciptaan, Gita
Department of Animal Feed and Nutrition, Faculty of Animal Science, Andalas University, Padang 25163, Indonesia http://orcid.org/0000-0002-0376-4247
Mirnawati, .
Department of Animal Feed and Nutrition, Faculty of Animal Science, Andalas University, Padang 25163, Indonesia http://orcid.org/0000-0002-9887-7227
Aini, Qurrata
Department of Animal Feed and Nutrition, Faculty of Animal Science, Andalas University, Padang 25163, Indonesia
Srifani, Anifah
Doctoral student, Department of Animal Feed and Nutrition, Faculty of Animal Science,
Andalas University, Padang 25163, Indonesia
Makmur, Malik
Post-doctoral fellow, National Research and Innovation Agency, Cibinong 16911, Indonesia http://orcid.org/0000-0002-5803-417X
veterinary sciences; animal sciences; poultry nutrition
Background: Soybean milk dregs (SMD) are one of the potential feed ingredients for quail, however improving their nutritional value and utilization requires a biotechnological approach. This investigation was performed on the percentage and effect of using fermented soybean milk dregs (FSMD) inoculated with Aspergillus ficuum in quail rations.
Methods: 200 quails (Coturnix japonica), aged 20 weeks, were used in this study. They were kept in 20 battery cage units, each with 10 quails inside. The analysis was carried out using a completely randomized design with 5 treatment rations and 4 replications. The treatment rations were R1, R2, R3, R4, and R5 using 0, 10, 15, 20, and 25% FSMD respectively, which were prepared based on 20% iso-protein and 2800 kcal/kg iso-calorie. Feed consumption, egg production, egg mass, feed conversion, egg weight, shell thickness, and quail yolk color were among the variables that were observed.
Results: According to the results of the analysis of variance, feed consumption, egg production, egg mass, feed conversion, egg weight, and shell thickness were all unaffected by the use of FSMD (P >0.05). Additionally, there was a strong correlation between parameters under the treatment effect, with the exception of egg thickness.
Conclusion: The optimum level of fermented soybean milk dregs was 25% level in quail rations.
Keywords: Soybean milk dregs; Aspergillus ficuum; Fermentation; Quail performance
The RunningLine
2024-03-06 16:36:17
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2022
Advancements in Life Sciences; Vol 11, No 1 (2024): Advancements in Life Sciences
eng
Copyright (c) 2024 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/123
2015-05-25T01:34:01Z
ALS:ART
nmb a2200000Iu 4500
"150525 2015 eng "
2310-5380
dc
Study of drug mediated effects in mice: Histology based findings
Altaf, Mehwish
University of Lahore
Hasnain, Sidra
Superior University Lahore
Ullah, Razi
Superior University Lahore
Khan, Wasim
Quaid-e-Azam University, Islamabad
Molecular Biology; Biochemistry; Biotechnology
Aackground: Drugs induce numerous kinds of pharmacological effects in different body organs. For protecting organs from damage and destruction from drugs, the study of such effects is extremely important. Rapidly accumulating experimental data has opened new horizons for a comprehensive re-conceptualization of chemical modulated changes in histological features of body tissues.
Methods: In this study we studied dextromethorphan, ethanol, methanol and midazolam induced changes in histological specimen from different organs in post-treated mice.
Results: No pathological changes were observed in heart, liver and kidney by administering dextromethorphan at a dose of 61 mg/kg. While, ethanol causes pathological changes in heart, liver and kidney tissue at high dose i.e. 2000 mg/kg. Gross Pathological changes were observed in heart, liver and kidney by giving midazolam at a dose of 200 mg/kg.
Conclusion: Dextromethorphan showed lesser side effects and is less toxic as compared with other drugs, such as, ethanol, methanol and midazolam. Lesser toxic effects were observed when drugs were administered alone, but in combination, these drugs produced higher toxic effects.
The RunningLine
2015-05-25 01:34:02
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/123
Advancements in Life Sciences; Vol 2, No 3 (2015): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/1063
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
Local Radiation Dosimetry Method using Optically Stimulated Pulsed Luminescence and Monte Carlo Simulation
Talbi, Mohammed
Moulay Ismail University
El Mansouri, M'hamed
Messaoud, Mounir Ben
Sebihi, Rajaa
Erraoudi, Morad
Azakhmam, Yassine
Khalis, Mohammed
physics department
Background: This is the first study that has been done in Morocco with the aim of optimizing protection and protocols in diagnostic radiology, by using Monte Carlo simulation and Optically Stimulated Luminescence (OSL).
Methods: Measurements have been performed with solid (AGMS-D+) and OSL detectors to determine the Air Kerma and the backscattering factor on a diagnostic radiology unit.
Results: The spectra simulated by GATE were in a good adequacy with spectra generated by IPEM report 78, with slight differences in the X-rays intensity characteristic, and there was no statistically significant difference between Air Kerma simulated with GATE and those measured using the AGMS-D+ and OSL (P < 0.01).
Conclusion: Monte Carlo simulation responses were suitable and could provide an accurate alternative for Air Kerma and the entrance surface dose determination with non‐uniform primary x‐ray beams.
Keywords: Radiation Dosimetry; Pulsed Luminescence; Monte Carlo Simulation
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1063
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1238
2023-07-15T04:31:43Z
ALS:ART
nmb a2200000Iu 4500
"230715 2023 eng "
2310-5380
dc
Assessment of heavy metal contamination in vegetables collected from selected localities of Okara, Pakistan
Younas, Saba
"University of Okara"
Tahira, Syeda Anjum
"University of Okara"
Farooq, Umer
"University of Okara"
Botany; Biotechnology; Biochemistry; Environmental sciences; food sciences
Background: Human health is prone to heavy metals especially which become part of food chain by any means. Previously, no extensive nutritional studies are conducted on local food products and grains. The research work was carried out to observe level of heavy metals in vegetables sold or consumed in different localities of Okara city, Pakistan.
Methods: The concentration of heavy metals Nickel, Cadmium, Cobalt, Copper and Chromium in ten different types of vegetables collected from selected sites survey were analyzed using tri-acid method for atomic absorption spectrometer.
Results: The results showed that concentrations of all analyzed heavy metals were significantly (P≤ 0.05) different, except values of copper in collected vegetable samples. The average concentration ranged from 5.4 – 44.06 ppm of Cobalt, 5.7 – 9.63 ppm of Copper, 4.49 – 11.13 ppm of Cadmium, 4.59 – 33.77 ppm of Chromium and 8.58 – 13.68 ppm of Nickel. Mean concentrations of metals were found in following sequence Copper < Cadmium < Nickel < Chromium < Cobalt.
Conclusion: It was concluded that vegetables can accumulate high concentrations of Cobalt and Chromium while Nickel, Cadmium and Copper concentrates in low amount, from which level of chromium and cadmium surpasses the permissible limits by FAO/WHO especially in underground vegetables (Onion and Radish), which could be the cause of serious health issues. Hence, monitoring and assessment of contaminants in vegetables are periodically needed and public safety measures should be imposed.
Keywords: Heavy metal; Bio-concentration; Vegetables; Pakistan
The RunningLine
2023-07-15 04:31:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1238
Advancements in Life Sciences; Vol 10, No 2 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/370
2017-11-25T20:04:53Z
ALS:ART
nmb a2200000Iu 4500
"171125 2017 eng "
2310-5380
dc
GENETIC DIVERSITY AND PHYLOGENETIC RELATIONSHIP IN DIFFERENT GENOTYPES OF COTTON FOR FUTURE BREEDING
Bakht, Jehan
Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar
Iqbal, Marina
Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar
Iqbal, Marina
Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar
Shafi, Mohammad
Department of Agronomy, The University of Agriculture Peshawar
Shafi, Mohammad
Department of Agronomy, The University of Agriculture Peshawar
Molecular Biology; Biotechnology
Back ground: To make the plants well adapted and more resistant to diseases and other environmental stresses there is always a need to improve the quality of plant’s genome i.e. to increase its genetic diversity.
Methods: In the present study six variety and six lines of cotton were investigated for their genetic diversity and phylogenetic relationship. For this purpose 35 different RAPD primers obtained from the Gene Link Technologies,USA were used.
Results: Among 35 RAPD primers, 13 primers produced reproducible PCR bands while the rest failed to show any amplification product. Our results indicated that the total count of the reproducible bands was 670 and polymorphic loci were counted to be 442 which constitute 66% of total loci. Phylogenetic analysis revealed two major groups each consists of 7 and 5 genotypes respectively. Genotypes Lp1 and Tp4 were placed at maximum genetic distance and in separate groups and could be utilized for future cotton breeding.
Conclusions: RAPD analysis is a cheaper and time saving technique for the determination of genetic diversity of different cotton genotypes. Cotton genotype Lp1 and Tp4 could be the best candidates for future breeding programs as both genotypes are genetically distant from each other.
The RunningLine
2017-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/370
Advancements in Life Sciences; Vol 5, No 1 (2017): Advancements in Life Sciences
eng
Copyright (c) 2017 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1019
2022-05-29T08:45:07Z
ALS:ART
nmb a2200000Iu 4500
"220529 2022 eng "
2310-5380
dc
Antimicrobial Resistant Pattern of Isolates from Intensive Care Unit of Tertiary Care Hospital
Ahmad, Shehryar
Lecturer ,College of Medical Technology
Bacha khan Medical College Mardan
Rasheed, Ujala
Rahman College of Allied Health Sciences - Pakistan
Naz, Iram
National University System - Pakistan
Ali, Sajid
Medical Oncology-Hayatabad Medical Complex - Pakistan
Ali, Nasir
College of Medical Technology- Bacha Khan Medical College - Pakistan
Aziz, Atif
Medical Emergence Resilience foundation – Pakistan
Microbiology;
Background: Patients in intensive care units are more vulnerable and have a higher risk of Hospital acquire infection in comparison to non-critical patient’s wards. We determine the Microbial spectrum and their antimicrobial resistant pattern of isolates from intensive care units of tertiary care hospital in Peshawar, Pakistan.
Methods: A cross-sectional study was carried out for a period of 6 months and a total of 65 patients from the surgical intensive care unit and medical intensive care unit of tertiary care hospital were sampled for the possible pathogen. Non-probability convenient sampling techniques were used to enrolled patients and samples were transported to the pathology department for culture and anti-microbial resistant pattern. Data were analyzed on SPPS version 19 using descriptive and inferential statistical tools. Chi-square test were used for possible association and p value of < 0.05 were considered statistically significant.
Results: Both gram-positive and gram-negative isolates additional to fungi were prevalent in the intensive care unit with the most frequent isolates being Acinetobacter species followed by Enterobacteriaceae and P. Aeruginosa respectively. Amikacin, Doxycycline, Linezolid, Tigecycline, and Vancomycin have a low resistant pattern for both Staph aureus and coagulase-negative staphylococci (CoNS) while Cephalosporin including cefepime (58%), and minocycline (35%) along with polymyxin B shows the lower resistance pattern for Acinetobacter and other gram-negative isolates.
Conclusion: Tigecycline, vancomycin, cefepime, and polymyxin B were used as empiric therapy agents in severe conditions however a larger scale study is required for calculating optimized therapeutic regime for each agent. The physician and hospital guidelines should adhere to the monitoring of antimicrobials to limit the upsurge of resistance patterns among different pathogenic organisms.
Keywords: Anti-Microbial; Resistant; Intensive care unit; Hospital acquire Infection
The RunningLine
2022-05-29 08:45:07
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1019
Advancements in Life Sciences; Vol 9, No 1 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1861
2023-11-02T15:34:59Z
ALS:ART
nmb a2200000Iu 4500
"231023 2023 eng "
2310-5380
dc
The effects of 4β-hydroxy withanolide E extracted from Physalis Peruviana on Complete Blood Count of Dimethylbenz(a)anthracene-induced Breast Cancer in Albino Rats
Gul, Fahmida
Liaquat University of Medical and Health Sciences, Jamshoro, Sindh, Pakistan http://orcid.org/0000-0002-9566-1105
Memon, Samreen
Liaquat University of Medical & Health Sciences Jamshoro
Ujjan, Ikramuddin
Liaquat University of Medical & Health Sciences Jamshoro
Goswami, Pushpa
Professor Department of Anatomy Liaquat University of Medical & Health Sciences, Jamshoro.
Bhatti, Kanwal Abbas
Liaquat University of Medical & Health Sciences Jamshoro
Molecular Biology; Biochemistry;
Background: Several studies reveal changes in blood parameters in patients with cancer. Complete blood count is a good predictor for many cancer diagnoses and prognoses. They are also helpful in assessing drug efficacy and toxicity. Cancers may be treated with bioactive and antioxidant compounds derived from plants. The effects of Physalis peruviana on the complete blood count of dimethylbenz(a)anthracene induced are still not well identified.
Methods: This study was conducted at Liaquat University of Medical and Health Sciences, Jamshoro, for six months on 60 female albino rats divided in 05 groups. The effects of Physalis peruviana fruits and tamoxifen on complete blood count in dimethylbenz(a)anthracene induced breast cancer were assessed. Blood samples were collected at weeks 1, 5, 7, and 10 for hematological changes before, during, and after treatment.
Result: Rats of group E (Tumor-induced albino rats treated with the extracts of Physalis Peruviana and tamoxifen) gave the best results compared to other groups. In this group, increases in weight along with an improvement in hematological parameters were observed when compared with others.
Conclusion: The extract from Physalis Peruviana helps in the reversal of pancytopenia with anemia and thrombocytopenia caused by tumor-inducing drugs.
The RunningLine
2023-10-23 16:40:41
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1861
Advancements in Life Sciences; Vol 10, No 3 (2023): Advancements in Life Sciences
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/717
2019-08-25T00:54:00Z
ALS:ART
nmb a2200000Iu 4500
"190825 2019 eng "
2310-5380
dc
Effect of yeast supplementation on production parameters, egg quality characteristics and crude protein digestibility in hens
Hameed, Rohban
Department of Animal Nutrition,
Faculty of Animal Husbandry and Veterinary Sciences,
University of Agriculture, Peshawar, Pakistan
Tahir, Muhammad
Department of Animal Nutrition,
Faculty of Animal Husbandry and Veterinary Sciences,
University of Agriculture, Peshawar, Pakistan
Khan, Sohail Hassan
Poultry Research Institute, Murree Road, Shamsabad, Rawalpindi, Pakistan
Ahmad, Tanveer
Department of Livestock Production and Management,
Faculty of Veterinary and Animal Sciences,
Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
Iqbal, Javid
Department of Poultry Science,
Faculty of Veterinary and Animal Sciences,
Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
Background: Yeast (Saccharomyces cerevisiae) has a buffering effect in the digestive tract, and proper feed additives in chicken diets can improve the birds’ digestive efficiency. The present study planned with aim to investigate the effects of different levels of yeast supplementation on the egg production, egg quality traits and crude protein digestibility in hens.
Methods: A total of 84 hens (40 weeks of age) were fed the dietary treatments until 50 weeks of age. The hens were distributed into 4 treatments and 3 replicates with 7 hens per experimental unit. The yeast was incorporated into basal feed with various levels (0, 0.1, 0.15 and 0.2%). The production data was obtained during trial. For determining egg quality characteristics, 30 eggs from each treatment group were used at 14-day interval of the experiment from a 2-day collection of eggs during the week. The excreta from each replicate were collected after every 2 weeks for determining the crude protein digestibility.
Results: Maximum egg production and egg mass were attained from the hens provided 0.15% and 0.20% yeast supplementation. The eggshell thickness decreased with supplementation of yeast. Higher yolk weight, albumen weight, Haugh unit and grading values were observed at 0.2% level than other groups. Crude protein digestibility also improved at high levels of yeast supplementation at all ages.
Conclusion: This study envisaged that yeast supplementation between 0.15 and 0.20% could be sufficient during 40 to 50 weeks of age, to augment the overall layer performance.
Keywords: Baker’s yeast; feed intake; egg production; egg quality
The RunningLine
2019-08-25 00:54:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/717
Advancements in Life Sciences; Vol 6, No 4 (2019): Advancements in Life Sciences
eng
Copyright (c) 2019 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1483
2022-11-05T03:24:51Z
ALS:ART
nmb a2200000Iu 4500
"221104 2022 eng "
2310-5380
dc
Introduction of Large-Fruited Strawberry Varieties on the Territory of the Novosibirsk Region in the Conditions of Western Siberia
Makarova, KS
Novosibirsk State Agrarian University
Petrov, AF
Novosibirsk State Agrarian University
Pastukhova, AV
Novosibirsk State Agrarian University
Gazizulina, AS
Novosibirsk State Agrarian University
Petruk, VA
Siberian Federal Scientific Center for Agrobiotechnologies, Russian Academy of Sciences
Gotfrid, SS
Novosibirsk State Agrarian University
Background: For the Novosibirsk region, the need for planting material suitable for growing varieties is very high. The relatively easy technology of growing strawberries allows obtaining high yields not only for those who grow them but also on an industrial scale. Strawberry fruits contain a huge number of useful elements, including vitamins, which are necessary for the human body. The study aimed to determine the influence of the maturity group on the yield of introduced varieties of large-fruited garden strawberries.
Methods: Records and observations were carried out from May 2019 to September 2020 at the collection site of the biological polygon of the Federal State Budgetary Research Institution Siberian Physical and Technical Institute in the Novosibirsk Region.
Results: During the years of the study, the weather conditions of the spring period developed successfully for the growth and development of strawberries. Thus, on average, over the years of the study, high yields were shown of different maturity groups.
Conclusion: The obtained yield indicators obtained were not inferior to previously zoned and local varieties, which shows that planting can be carried out both in traditional soil and in soilless culture. Thus, the quality and quantity of fruits does not change with the physicochemical properties or methods of cultivation but depends on the age of the plant and the seasonal crop cycle.
The RunningLine
2022-11-04 09:11:57
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1483
Advancements in Life Sciences; Vol 9, No 3 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/2173
2023-12-14T08:18:51Z
ALS:ART
nmb a2200000Iu 4500
"231214 2023 eng "
2310-5380
dc
Single Nucleotide Gene Polymorphism of Toll-Like Receptor 2 to Toxoplasmosis in Recurrent Aborted Women
Aubed, Mohammed Hassan
College of Medicine, University of Al-Qadisiyah
Alomashi, Ghada Basil Ali
College of Medicine, University of Al-Qadisiyah
Background: This study was conducted at the College of Medicine – Al-Qadisiyah University in Cooperation with the women and children in Wasit hospitals from the period January to October -2022. It has been carried out on 102 blood samples from aborted women and congenital anomalies mothers referred to the hospitals 50 blood samples from pregnant women as control cases, and 50 blood samples as a control group obtained from healthy pregnant women. The objective was to determine the effect of TLR2 gene polymorphism on seropositive toxoplasmosis in aborted women
Methods: The research aimed to explore how the (TLR2) gene polymorphism influences the occurrence of miscarriages in women. This was investigated using the ARMS-PCR technique, which utilized allele primers to identify Toxoplasma gondii infection in women who experienced clinical miscarriages. The study also assessed the genotypes for the TLR2 G/A (rs1898830) SNPs through ARMS PCR assays, and all genotypes were evaluated for compliance with Hardy-Weinberg (H-W) equilibrium.
Results: All the analyzed genotypes preserved the H-W equilibrium among the studied groups of patients (P >0.942 and 0.893 ). The result of gene polymorphism of (TLR2, SNP rs-1898830) in Toxoplasma aborted women, showed that the homozygote mutant AA appeared 19, and heterozygote mutant GA appeared in 53was a risk factor for abortion in TLR2, and the difference was non-significant.
Conclusion: The current study concluded that genetic polymorphisms of TLR-2 had a risk factor role in toxoplasma patients, which many correlated with aborted women, rather than pregnant women.
The RunningLine
2023-12-14 08:18:51
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/2173
Advancements in Life Sciences; Vol 10 (2023): Advancements in Life Sciences - Perspectives in Iraqi Life Sciences: Recent Developments and Prospects - Special Issue 10(S1)
eng
Copyright (c) 2023 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/958
2020-06-01T03:39:43Z
ALS:ART
nmb a2200000Iu 4500
"200601 2020 eng "
2310-5380
dc
Study of diagnostic and prognostic parameters in Breast cancer patients without metastasis
Neelam, Aqsa
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan
Ali, Ahmad
National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad
Alam, Rabail
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan
Mujtaba, Ghulam
Institute of Nuclear Medicine and Oncology, Lahore
Malik, Imran Riaz
Assistant Professor
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Biochemistry
Background: Carcinoma of the breast is the most prevalent cause of mortality from cancer in women aged 40-69 years. The aim of the present study was to examine any alteration in the status of lactate dehydrogenase, ferritin, gamma glutamyl transferase, Platelets, hemoglobin, alkaline phosphatase, aspartate transaminase, alanine transferase and bilirubin in the plasma of breast cancer patients without distant metastasis, to establish their diagnostic and prognostic values.
Methods: Current reading describes a study conducted on 50 breast cancer patients from Institute of Nuclear Medicine and Oncology (Lahore). The patients were clinically categorized as stage II (19 patients) and stage III (29 patients) respectively. Most of the patients were diseased with infiltrative ductal carcinoma (48) of the breast. Blood samples of all patients were collected after forty days of chemotherapy course besides 10 healthy subjects.
Results: There was a significant rise in LDH (221.48 ± 18.44) and Ferritin (356.46 ± 12.28) levels as compared to control groups. Concentration of GGT (34.12 ± 1.61) was also elevated but not significantly raised. There was no significant rise in ALP (279.27 ± 26.60), AST (49.44 ± 8.510), ALT (33.40 ± 2.83), bilirubin (4.87 ± 3.96), Hb (11.16 ± .299) and platelets (032.54 ± 21.68) levels in comparison to normal control subjects. An elevation of LDH and ferritin levels in cases of carcinoma breast signifies its importance as a biomarker of disease. A serial measurement of these enzymes would have diagnostic and prognostic significance and help treatment decisions.
Conclusion: The ferritin along with lactate dehydrogenase can be used as a valuable biomarker for breast cancer diagnosis and prognosis.
Keywords: Breast cancer; Prognostic parameters; Treatment; Metastasis
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2020-06-01 03:39:43
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/958
Advancements in Life Sciences; Vol 7, No 3 (2020): Advancements in Life Sciences
eng
Copyright (c) 2020 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/1290
2023-01-21T22:12:11Z
ALS:ART
nmb a2200000Iu 4500
"230121 2023 eng "
2310-5380
dc
Effect of Cadmium and Copper on Morphological and Physiological Parameters of Stevia rebaudiana Bert. Under in Vitro Conditions
Shahzadi, Afshan
University of the Punjab, Lahore, Pakistan
Altaf, Ayishm
Institute of Botany, University of the Punjab, Lahore, Pakistan
Afrasiab, Humera
Institute of Botany, University of the Punjab, Lahore, Pakistan
Biotechnology
Background: Stevia rebaudiana Bertoni is a popular non-caloric sweetener being used all over the world for its anti-diabetic properties. Stevia contains phytochemical compounds known as diterpene glycosides that helps to reduce blood sugar, cholesterol, and blood pressure. In vitro raised cultures are considered as an efficient way to produce essential secondary metabolites, especially through elicitation. This study aims to optimize culture conditions for the large-scale production of these economically significant metabolites using abiotic elicitors.
Methods: Apical shoot, nodes as well as leaf explants from Stevia mother plants were used for micropropagation and callogenesis respectively. Murashige and Skoog (MS) medium supplemented with different cytokinins and auxins alone or in combinations were tried for obtaining the maximum yield of calli and plantlets. The calli and plantlets were subjected to different concentrations of copper sulphate and cadmium chloride. After 40 days of treatment, the data was recorded for morphological characters of elicitor-treated plants and calli. Chlorophyll content and antioxidant activity using DPPH assay were performed using treated leaves and calli extracts.
Results: Among all the cytokinins tried for micropropagation, BAP at 0.5 mg/L in MS medium proved to be the best with 100% response by inducing 34 and 40 shoots/culture within 12 days of inoculation from shoot tip and nodal explants, respectively. For rooting, MS medium containing 0.6 mg/L of IBA was best producing 15-17 roots/culture within 10 days. Green friable and embryogenic calli was obtained in MS medium containing 1.0 mg/L TDZ with 0.1 mg/L NAA from leaf explants. A significant increase was observed in the elicitors-treated calli and plantlets biomass, chlorophyll content as well as antioxidant potential as compared to the control.
Conclusion: The results of this study indicate that the cytokinins and auxins at lower concentrations gave significant results for micropropagation and callogenesis. Further, elicitation with copper sulphate and cadmium chloride has improved the biomass and antioxidant activity of calli and plantlets.
Keywords: Stevia; Micropropagation; Callus induction; Elicitation; Antioxidant activity; Biomass
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2023-01-21 22:12:11
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/1290
Advancements in Life Sciences; Vol 9, No 4 (2022): Advancements in Life Sciences
eng
Copyright (c) 2022 Advancements in Life Sciences
oai:ojs.submission.als-journal.com:article/86
2014-11-30T01:20:52Z
ALS:ART
nmb a2200000Iu 4500
"141125 2014 eng "
2310-5380
dc
Phylogenetic analysis of coat protein gene of CYDV-RPV strain from Wheat
Zamurrad, Madiha
PMAS-ARID AGRICULTURE UNIVERSITY RAWALPINDI
Hameed, Shahid
CROP DISEASE RESEARCH INSTITUTE, NARC ISLAMABAD
Saleem, Kamran
PLANT PROTECTION DIVISION, NUCLEAR INSTITUTE FOR AGRICULTURE AND BIOLOGY (NIAB) P.O.BOX-128, JHANG ROAF FAISALABAD
Haque, Irfan ul
PMAS-ARID AGRICULTURE UNIVERISTY
Kausar, Samra
ADAPTIVE RESEARCH FARM CHAKWAL
PLANT PATHOLOGY, PLANT VIROLOGY
Background: Keeping in view the potential damage caused by viruses to production of different crops and possible ‘directed damages’ by manipulated viral attack in/across border collectively make phylogenetic analysis of any attacking viral specie important. Cereal yellow dwarf viruses (CYDV) are highly important viruses in wheat causing significant yield loss.
Methods: Double antibody sandwich ELISA and reverse transcription polymerase chain reaction (RT-PCR) was used to detect and confirm the polerovirus i.e. CYDV-rhopalosiphum padi virus (RPV), and unassigned viruses (SGV, RMV) in Punjab and NWFP provinces. The PCR products were inserted into a pGEM®-T easy vector, which then transformed in JM-107 cells of Escherichia coli. Recombinant plasmids were sequenced. Nucleotide and predicted amino acid sequences were aligned, analyzed and compared with other RPV isolates of the family. The nucleotide sequence data were used to make a phylogenetic tree.
Results: Sequencing of 600 bp of coat protein gene confirmed the presence of CYDV-RPV strain. Pakistani isolate has close phylogenetic relationship with RPV-Mexcio and RPV-Yolo (USA). They had 99.95% similarity with RPV-Pakistan. The RPV-Aus, RPV-IR, and RPV-Cal (USA) had 99.94% identities with RPV-Pakistan.
Conclusion: This work led to a conclusion that there is very low genetic diversity in RPV-Pakistan. Now it is in our future interest to clarify the identity of RPV-PK with more sequencing. The current study may help scientists to formulate appropriate management strategies against CYDV-RPV.
The RunningLine
2014-11-25 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/86
Advancements in Life Sciences; Vol 2, No 1 (2014): Advancements in Life Sciences
eng
Copyright (c)
oai:ojs.submission.als-journal.com:article/904
2021-08-04T19:18:33Z
ALS:ART
nmb a2200000Iu 4500
"210313 2021 eng "
2310-5380
dc
Salinity, livelihood and agricultural productivity: A case of Hafizabad District
Malik, Arshad Mahmood
Director Institute of hydroponics Agriculture, PMAS Arid Agriculture University Rawalpindi http://orcid.org/0000-0001-5028-7482
Tayyab, Hafiz Muhammad
Arid Agriculture University Rawalpindi
Ullah, Muhammad Arshad
Pakistan Agriculture Research Council, Islamabad
Bilal, Muhammad Talha
COMSATS University, Islamabad
Salinity and Livelihood Analysis
Background: Soil salinity; concentration and or accumulation of salts may pose severe risk on soil productivity and in turn concerned population and farmers. Salt-influenced lands in Pakistan were spread over 6.63 million hectare which is diminishing the agrarian profitability. This study will explore the impact of salinity on livelihood of farmers in district Hafizabad.
Methods: Data of 192 small, medium and large farmers was collected from four randomly selected villages of salinity affected area of Hafizabad district of Punjab province of Pakistan using multistage probability sampling technique. SPSS version 21.0 was utilized to analyze the data for generating logical results.
Results: Farmers belonging to saline area communities were characterized on the basis of their education, experience, cultivated area, and method of irrigation and technological adoption for analyzing their livelihood typologies. Average yield of wheat was found to be 26mnds/acre, while marketable surplus was high for large farmers due to ownership of more area. Livelihood typologies were derived mainly from on-farm and off-farm income activities of the farmers. Agriculture farm earning in the saline area was estimated as Rs. 10 to 12 thousand per acre. Contribution of off-farm income in household cash flows was estimated in 79% of small, medium and large farmer as less than 15000 indicating the dependency status of the households.
Conclusion: Major livelihood source in Salt-affected soils was still agricultural cash inflows beside their contribution to the food basket of consumers. Farmers were in favour of provision of farming inputs on subsidized rates i.e. lime and gypsum as a poverty alleviation strategy in the area for positive promotion of sharing culture with public sector.
Keywords: Salinity; Livelihood; Productivity; Farming experience; Farm size
The RunningLine
2021-03-13 00:00:00
Peer-reviewed Article
application/pdf
https://submission.als-journal.com/index.php/ALS/article/view/904
Advancements in Life Sciences; Vol 8, No 2 (2021): Advancements in Life Sciences
eng
Copyright (c) 2021 Advancements in Life Sciences
b253d514d333469bc927409d25768782