Biosecurity and Biosafety concerns of Research and diagnostic Laboratory under International Guidelines

Noor-ul- Huda, Fabiha Shahid, Iqra Iftikhar, Hafiz Muhammad Rehman, Shehla Javaid, Mubeen Fatima, Iqra Rehman, Momal Babar


Currently, academic research labs and diagnostic laboratories are facing a serious issue of biosecurity and biosafety globally. The high rate of mutations and continuous new emerging infectious diseases with the risk of bioterrorism demands that each lab personnel share equal responsibility for biosecurity & biosafety at their work. The primary job of government authorities is to implement a well-organized detection system and limit the spread of hazardous biological agents by providing a biologically safe setting for lab scientists and for the common man in communities and institutes. The safety of lab personnel, lab environment, and pathogens depends on effective and safe laboratory working and pathogen handling which determines the reliable and accurate results of laboratory experiments. The aim of this article is to address the basic components of laboratory biosafety, laboratory biosecurity, and laboratory management. Further DURC (dual-use research of concern) deals with the commencement of lawful reasons to generate information, knowledge, technology, and products that are used for either harmful or beneficial purposes. The precautions are taken to ensure laboratory biosecurity and biosafety should be a part of the laboratory safety policy manual for guidance and implementation for a safe laboratory environment.

Keywords: Antioxidants; tomato; lycopene; β-carotene; reactive oxygen species (ROS) 

Full Text:



Muneer S, Kayani HA, Ali K, Asif E, Zohra RR, et al. Laboratory biosafety and biosecurity related education in Pakistan: Engaging students through the Socratic method of learning. Journal of Biosafety and Biosecurity, (2021); 3(1): 22-27.

Zaki AN. Biosafety and biosecurity measures: management of biosafety level 3 facilities. International journal of antimicrobial agents, (2010); 36: S70-S74.

Biorisk management: Laboratory biosecurity guidance: World Health Organization; 2006.

Karthik K, Babu RPA, Dhama K, Chitra MA, Kalaiselvi G, et al. Biosafety concerns during the collection, transportation, and processing of COVID-19 samples for diagnosis. Archives of Medical Research, (2020); 51(7): 623-630.

Peng H, Bilal M, Iqbal HM. Improved biosafety and biosecurity measures and/or strategies to tackle laboratory-acquired infections and related risks. International journal of environmental research and public health, (2018); 15(12): 2697.

Laboratory biosafety manual: World Health Organization; 2004.

Shrivastava SR, Shrivastava PS. COVID-19 pandemic: Responding to the challenge of global shortage of personal protective equipment. Social Health and Behavior, (2020); 3(2): 70.

Laboratory biosafety guidance related to coronavirus disease 2019 (‎COVID-19)‎: interim guidance, 12 February 2020.

Kalam A, Asif E, Tabassum S. Biosafety and Biosecurity Measures in Clinical/Research Laboratory: Assistance with International Guidelines: Laboratory Biosafety and Biosecurity Guidelines. Proceedings of the Pakistan Academy of Sciences: B Life and Environmental Sciences, (2021); 58(S): 31-35.

Centers for Disease Control and Prevention, National Institutes of Health, Biosafety in microbiological and biomedical laboratories. Washington DC: US Government Printing Office; 1999.

Ta L, Gosa L, Nathanson DA. Biosafety and biohazards: understanding biosafety levels and meeting safety requirements of a biobank. Biobanking, (2019); 213-225.

Emmert EA, Biosafety ATCoL. Biosafety guidelines for handling microorganisms in the teaching laboratory: development and rationale. Journal of microbiology & biology education, (2013); 14(1): 78-83.

Cleveland JL, Gray SK, Harte JA, Robison VA, Moorman AC, et al. Transmission of blood-borne pathogens in US dental health care settings: 2016 update. The Journal of the American Dental Association, (2016); 147(9): 729-738.

Rational use of personal protective equipment for COVID-19 and considerations during severe shortages: interim guidance, 23 December 2020. World Health Organization; 2020.

Laboratory testing for coronavirus disease 2019 (‎ COVID-19)‎ in suspected human cases: interim guidance, 2 March 2020. World Health Organization; 2020.

Schuchat A, Anderson LJ, Rodewald LE, Cox NJ, Hajjeh R, et al. Progress in vaccine-preventable and respiratory infectious diseases—first 10 years of the CDC National Center for Immunization and Respiratory Diseases, 2006–2015. Emerging infectious diseases, (2018); 24(7): 1178.

Joliat JN. National Science Advisory Board for Biosecurity (NSABB). Encyclopedia of Bioterrorism Defense, (2005); 1-7.

Boyd-Anderson K. US Creates Advisory Board for Biosecurity. Arms Control Today, (2004); 34(3): 36.

Biorisk management: Laboratory biosecurity guidance, (2006); World Health Organization.

Salerno RM, Gaudioso J, Brodsky BH. Laboratory biosecurity handbook: CRC press; 2007.

Kyriacou DN, Dobrez D, Parada JP, Steinberg JM, Kahn A, et al. Cost-effectiveness comparison of response strategies to a large-scale anthrax attack on the Chicago metropolitan area: impact of timing and surge capacity. Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science, (2012); 10(3): 264-279.

Fe S. Bioinsecurity and Vulnerability. (2014).


  • There are currently no refbacks.