Prevalence of MRSA colonization among healthcare-workers and effectiveness of decolonization regimen in ICU of a Tertiary care Hospital, Lahore, Pakistan

Sameena Parveen, Sheeba Saqib, Altaf Ahmed, Aqeel Shahzad, Naveed Ahmed

Abstract


Background: The objectives of this study were to evaluate the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in the nasal cavity and to determine the effectiveness of decolonization regimen among health care workers working in the Intensive care unit (ICU) at a Tertiary care hospital in Lahore, Pakistan.

Methods: A cross-sectional study was carried out during the period of June, 2018 to August, 2018. A total of 46 healthcare workers were screened to see the prevalence of MRSA in the nasal cavity. Samples from the nasal cavity were collected using sterile swabs, and processed for bacterial cultures. The samples were inoculated on Blood agar and chocolate agar and then incubated at 37℃ for 24 hours. After the incubation period, petri plates were checked for the growth of S. aureus (MRSA). After identification of colonizers, five days decolonization regimen of nasal mupirocin ointment and Chlorhexidine based body wash was used and repeated cultures were done to check the effectiveness of this regimen. IBM SPSS version 25 was used to check the statistically significant relationship between prevalence of MRSA colonization with profession and gender.

Results: Among the total 46 participants, 6 (13%) were found MRSA colonizers, 12 (26.08%) showed Methicillin-Susceptible Staphylococcus aureus (MSSA) and remaining 28 (60.8%) showed no growth of S. aureus. The prevalence of MRSA colonization was found highest in nurses i.e. 66.67%, 16.67% in doctor and 16.67% were in respiratory therapists. The success rate of treatment regimen was 83.33%.

Conclusion: Results of the present study showed that the prevalence of MRSA was high in our setting but effectiveness of decolonization treatment regimen is similar to the worldwide success rate.

Keywords: MRSA; healthcare-workers (HCWs); Colonization; Decolonization


Full Text:

PDF

References


Ahmed N. Gender Based Prevalence of Antibiotic Resistance Patterns in Staphylococcus Aureus. Annals of Life Sciences, (2019); 223-31.

Shrestha B, Pokhrel BM, Mohapatra TM. Staphylococcus aureus nasal carriage among health care workers in a Nepal Hospital. Brazilian Journal of Infectious Diseases, (2009); 13(5): 322-322.

Rongpharpi SR, Hazarika NK, Kalita H. The prevalence of nasal carriage of Staphylococcus aureus among healthcare workers at a tertiary care hospital in assam with special reference to MRSA. Journal of Clinical Diagnostic Research, (2013); 7(2): 257.

Sohail M, Latif Z. Molecular typing of Methicillin Resistance Staphylococcus aureus (MRSA) isolated from device related infections by SCCmec and PCR-RFLP of coagulase gene. Advancements in Life Sciences, (2018); 6(1): 34-40.

Kohler P, Bregenzer-Witteck A, Rettenmund G, Otterbech S, Schlegel M. MRSA decolonization: success rate, risk factors for failure and optimal duration of follow-up. Infection, (2013); 41(1): 33-40.

Buehlmann M, Frei R, Fenner L, Dangel M, Fluckiger U, et al. Highly effective regimen for decolonization of methicillin-resistant Staphylococcus aureus carriers. Infection Control Hospital Epidemiology, (2008); 29(6): 510-516.

Alreshidi MM. Amino acid consumption and secretion patterns of Staphylococcus aureus following growth in sub-optimal environmental conditions. Advancements in Life Sciences, (2020); 7(2): 98-105.

Kluytmans J, Harbarth S. Methicillin-Resistant Staphylococcus aureus Decolonization “Yes, We Can,” But Will It Help? Infection Control Hospital Epidemiology, (2009); 30(7): 633-635.

Kumari N, Patoli BB, Patoli AA, Jabeen S. Biocontrol of MRSA and E. coli using bacteriophages from cow manure. Advancements in Life Sciences, (2020); 7(4): 264-269.

Goyal N, Miller A, Tripathi M, Parvizi J. Methicillin-resistant Staphylococcus aureus (MRSA) colonisation and pre-operative screening. The bone joint journal, (2013); 95(1): 4-9.

Elie-Turenne M-C, Fernandes H, Mediavilla JR, Rosenthal M, Mathema B, et al. Prevalence and characteristics of Staphylococcus aureus colonization among healthcare professionals in an urban teaching hospital. Infection Control Hospital Epidemiology, (2010); 31(6): 574-580.

Asghar M, Asghar N, Mumtaz S, Khan SA, Munir AH, et al. Frequency of methicillin resistant staphylococcus aureus (MRSA) colonization amongst hospital staff in teaching hospitals of Peshawar. Journal Of Medical Sciences, (2016); 24(4): 194-198.

Salman MK, Ashraf MS, Iftikhar S, Baig MAR. Frequency of nasal carriage of Staphylococcus Aureus among health care workers at a Tertiary Care Hospital. Pakistan journal of medical sciences, (2018); 34(5): 1181.

Boyce J. MRSA patients: proven methods to treat colonization and infection. Journal of Hospital Infection, (2001); 48S9-S14.


Refbacks

  • There are currently no refbacks.