Effect of Plant Growth Hormones on Shoot and Root Regeneration in Rose under In Vitro Conditions

Mehran Ali Chhalgri, Muhammad Tahir Khan, Ghulam Shah Nizamani, Shafquat Yasmeen, Imtiaz Ahmed Khan, Muhammad Mahran Aslam, Asghar Ali Rajpar, Tayyaba Tayyaba, Faiza Nizamani, Muhammad Rashid Nizamani, Rashid Iqbal, Mah Jabeen Panhwar, Mohammad Aquil Siddiqui


Background: Rose is a commercially important shrub. This research aimed to observe the influence of different plant growth hormones on development of shoots and roots of Rosa Indica Lin tissue culture.

Methods: Various concentrations of N6-Benzylaminopurine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) were used in the study. The different concentrations of BAP (2.00, 3.00, and 4.00 mg l-1) and IAA (2.00 and 3.00 mg l-1) were tested for shoot induction. While varying concentrations of IAA and IBA were analyzed for root proliferation.

Results: The results of the study indicated that the fastest shoot initiation (17.77 days), the highest number of shoots bottle-1 (3.55), the maximum shoot length (4.72 cm), and the utmost number of leaves bottle-1 (53.67) were observed on MS media containing 3.00 mg l-1 BAP, 3.00 mg l-1 IAA, and 30 g l-1 sugar, while the highest number of shoots bottle-1 were produced under MS + 2.00 mg l-1 BAP + 3.00 mg l-1 IAA + 30 g l-1 sugar. Regarding root induction, the maximum number of roots (4.67) and root length (2.60 cm) were observed under half strength MS media supplemented with 30 g l-1 sugar.

Conclusion: The study suggested that MS media containing 3.00 mg l-1 BAP and IAA could be used for tissue culturing rose plants. For root induction, half-strength MS media, along with sugar, could be used. The study gives an insight into potential media compositions for the propagation of rose. The suggested media can have promising uses in commercial multiplication of this important plant.

Keywords: Rose; Tissue culture; Micropropagation; MS media; Shoot development

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