DNA Fingerprinting and Cultivar Identification of Olive (Olea europaea L.) using SSR markers

Muhammad Zaffar Iqbal, Shakra Jamil, Rahil Shahzad, Sajid Ur Rahman


Background: Genetic diversity and population structure of the olive varieties cultivated in Pakistan are yet to be explored.

Methods: In present study, we studied population structure and genetic diversity and developed DNA fingerprints of 13 olive varieties  using 63 Simple Sequence Repeat  markers.

Results: Collectively 618 alleles were amplified among which 582 were polymorphic and 36 were monomorphic. High allelic diversity per locus was found among 63 SSR markers, i.e., one for GAPU-12 to 23 for UDO099-008 and GAPU-47 with an average 9.80 alleles per locus. On the basis of cluster analysis genotypes were grouped into two clusters. Cluster I contained varieties Manzanilla, Sohawa-selection, Koroneki, Bulkasar-selection, Arbequina, Arbosona, Chugtai-selection, whereas cluster II was comprised of varieties Bari-Zatoon, Coratina, Gemlik, Frontaio, Pendolino and Ottobratica. UDO-24 marker alone identified seven olive varieties. Similarly, DCA-07 and EMO-02 identified six olive varieties each.

Conclusion: The findings of this manuscript will be helpful for future studies related to DNA fingerprinting and genetic diversity assessment for choice of SSR markers and identification of olive varieties.   

Keywords: Cluster analysis; Polymorphic Information Content; Structure analysis; Similarity matrix 

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