Selection of potent bacterial strain for over-production of PHB by using low cost carbon source for eco-friendly bioplastics

Rahat Abdul Rehman, Abdul Qayum Rao, Zahoor Ahmed, Ambreen Gul


Background: The microbial PHB production is a promising tool for the plastic industry for the synthesis of environmental friendly, biodegradable plastic in contrast to the conventional petro-chemical based non-degradable plastics. The selection of potent bacterial strains, inexpensive carbon source, efficient fermentation and recovery processes are important aspects that were taken into account during this study.

Methods: Different bacterial strains i.e. Bacillus Spp, P. putida and P. fluorescens were screened for maximum PHB production. Under media optimization, various carbon and nitrogen sources (alone or in combination) were used to achieve the maximum PHB production. Finally the degradation tests of the PHB sheet were also performed to test its biodegradability potential.

Results: Shake flask studies have shown the PHB concentrations upto 7.02, 4.50 and 34.4 mg/g of dry cell mass of P. putida, P. fluorescens and Bacillus Spp. respectively. Almost same results were observed at laboratory scale production of PHB in 10 L fermenter i.e. 6.28, 6.23 and 39.5 mg/g of dry cell mass by P. putida, P. fluorescens and Bacillus Spp. respectively. On the basis of these observations, Bacillus Spp. was chosen for laboratory scale PHB production. Corn steep liquor (4%) was chosen as the best medium to achieve the highest PHB contents. Isolated PHB has shown biodegradation in soil up to 86.7% at 37oC.

Conclusion: The Bacillus Spp. Proved to be the best strain for PHB production on only 4% CSL which is cheapest and easily available.

Full Text:



Flechter A In: Plastics from Bacteria and for Bacteria: PHA as Natural, Biodegradable Polyesters. (1993); p 77-93. Springer Verlag, New York.

Raaz M, Bina R, Parihar S, Sharma A. Eco-friendly Bioplastic for Uncontaminated Environment. Research Journal of Chemical and Environmental Sciences, (2013); 1(1): 44-49.

Ronald M. Atlas RB Microbial Ecology: Fundamentals and Applications. (1993)

Barron K. Green Chemistry and Biodegradeable Plastics. ESSAI, (2011); 8(1): 7.

Lee S Y. 1996. Plastic bacteria? Progress and prospects for poly-hydroxyalkanoate production in bacteria. Trends. Biotechnol.14:431-438..

Disha N, Bhawana P, Fulekar MH. Production of Biodegradable Plastic from Waste Using Microbial Technology. International

Journal of Research in Chemistry and Environment, (2012); 2(2): 118-123.

Lauzier C, Revol J F and Marchessault, R H. Topotactic crystalization of isolated poly -3- hydroxybutyrate granules from Alcaligenes eutrophus. FEMS Microbiol. Rev, (1992); 103:299- 310.

Tyo, K E, Hang Z, and Gregory N. S. "High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803." Applied and environmental microbiology 72.5 (2006): 3412-3417.

Kumari P, Dhingra HK. Isolation and characterization of PHB producing micro-organisms isolated from root nodules of leguminous plants. The Bioscan, (2013); 8(1): 109-113.

Hahn SK, Chang YK, Kim BS, Chang HN. Optimization of microbial poly (3‐hydroxybutyrate) recover using dispersions of sodium hypochlorite solution and chloroform. Biotechnology and Bioengineering, (1994); 44(2): 256-261.

Reddy SV, Thirumala M, Mahmood S. Production of PHB and P (3HB-co-3HV) biopolymers by Bacillus megaterium strain OU303A isolated from municipal sewage sludge. World Journal of Microbiology and Biotechnology, (2009); 25(3): 391-397.

Mokhtari-Hosseini ZB, Vasheghani-Farahani E, Heidarzadeh-Vazifekhoran A, Shojaosadati SA, Karimzadeh R, et al. Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium. Bioresource Technology, (2009); 100(8): 2436-2443.

Vandamme P, Coenye T. Taxonomy of the genus Cupriavidus: a tale of lost and found. International Journal of Systematic and Evolutionary Microbiology, (2004); 54(6): 2285-2289.

Choi J-i, Lee SY, Han K. Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly (3-hydroxybutyrate) in Escherichia coli. Applied and Environmental Microbiology, (1998); 64(12): 4897-4903.

Chen G-Q, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials, (2005); 26(33): 6565-6578.

Valappil SP, Misra SK, Boccaccini AR, Keshavarz T, Bucke C, et al. Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV. Journal of Biotechnology, (2007); 132(3): 251-258.

Lopes MSG, Rocha RCS, Zanotto SP, Gomez JGC, da Silva LF. Screening of bacteria to produce polyhydroxyalkanoates from xylose. World Journal of Microbiology and Biotechnology, (2009); 25(10): 1751-1756.

Singh M, Patel SK, Kalia VC. Bacillus subtilis as potential producer for polyhydroxyalkanoates. Microbial Cell Factories, (2009); 8(1): 38.

Łabużek S, Radecka I. Biosynthesis of PHB tercopolymer by Bacillus cereus UW85. Journal of Applied Microbiology, (2001); 90(3): 353-357.

Tajima K, Igari T, Nishimura D, Nakamura M, Satoh Y, et al. Isolation and characterization of Bacillus sp. INT005 accumulating polyhydroxyalkanoate (PHA) from gas field soil. Journal of Bioscience and Bioengineering, (2003); 95(1): 77-81.

Ojumu T, Yu J, Solomon B. Production of polyhydroxyalkanoates, a bacterial biodegradable polymers. African Journal of Biotechnology, (2004); 3(1): 18-24.

Kim P, Kim J-H, Oh D-K. Improvement in cell yield of Methylobacterium sp. by reducing the inhibition of medium components for poly-β-hydroxybutyrate production. World Journal of Microbiology and Biotechnology, (2003); 19(4): 357-361.

Ishizaki A, Tanaka K, Taga N. Microbial production of poly-D-3-hydroxybutyrate from CO2. Applied Microbiology Biotechnology, (2001); 57(1-2): 6-12.

Nikel PI, Pettinari MJ, Méndez BS, Galvagno MA. Statistical optimization of a culture medium for biomass and poly (3-hydroxybutyrate) production by a recombinant Escherichia coli strain using agroindustrial byproducts. International Microbiology, (2010); 8(4): 243-250.

Nath A, Dixit M, Bandiya A, Chavda S, Desai A. Enhanced PHB production and scale up studies using cheese whey in fed batch culture of Methylobacterium sp. ZP24. Bioresource Technology, (2008); 99(13): 5749-5755.

RamKumar Pandian S, Deepak V, Kalishwaralal K, Rameshkumar N, Jeyaraj M, et al. Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3. Bioresource Technology, (2010); 101(2): 705-711.

Kim M-N, Lee A-R, Yoon J-S, Chin I-J. Biodegradation of poly (3-hydroxybutyrate), Sky-Green® and Mater-Bi® by fungi isolated from soils. European Polymer Journal, (2000); 36(8): 1677-1685.


  • There are currently no refbacks.