Molecular Identification of Cystic Echinococcosis in Mosul, Iraq

Sinan K. Abood, Najah S. N. Al-Omar

Abstract


Background: Cystic echinococcosis (CE) is a significant parasitic disease with substantial global economic and public-health impacts. It is caused by the larval form of Echinococcus granulosus.

Methods: The aim of this study was to identify the Echinococcus species in humans and livestock within Mosul, Iraq. This research work comprised fifty-five samples of viable Echinococcus cysts, including thirty from humans and twenty-five sheep. Human specimens were acquired from the main hospital in Mosul. Animal specimens were collected in the Al-Saadoon abattoir from June 2022 to March 2023.

Results: DNA samples were extracted from the protoscolex and blastoderm of each cyst sample. The mitochondrial 12S rRNA and cox1 genes were amplified by polymerase chain reaction. Gene sequencing and phylogenetic analyses were performed on 10 PCR-positive samples (5 from humans and 5 from sheep) selected by cyst size for laboratory feasibility.

Conclusion: The findings revealed that genotypes G1 and G3 were present in human echinococcosis cysts in Mosul while G1 genotypes were found in sheep. These findings highlight the need for integrated public-health and veterinary control measures.

Keywords: Echinococcus granulosus, Cystic echinococcosis, sensu stricto, Nineveh, Iraq


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DOI: http://dx.doi.org/10.62940/als.v12i3.1960

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