Assessment of Protein Klotho as Monitor in Diabetic Nephropathy

Sarah Sattar Jabber, Sanad Baqer Mahmmad

Abstract


Background: Klotho is a protein found throughout the body with higher concentrations in the kidneys. Specifically, as a person ages, the levels of Klotho decline gradually, correlating with the natural decrease in kidney function. As we age, a decline in klotho leads to various age-related illnesses like chronic kidney disease, hypertension, cancer, diabetes, and heart disease. Klotho plays a role in regulating metabolism, including glucose and fatty acids, as well as aiding in bile acid production.

Methods: A total of 135 subjects were enrolled in this study, comprising 90 patients with type 2 diabetes mellitus (T2DM) and 45 healthy controls. The diabetic patients were divided into three subgroups based on the level of albuminuria: macroalbuminuria (n = 22), microalbuminuria (n = 23), and normoalbuminuria (n = 45). Serum Klotho levels were measured using the ELISA technique, along with other kidney function tests. The study was conducted at the Hospital of Telafer, Mosul, Iraq, from November 2021 to April 2022.

Result: Compared with controls, diabetic nephropathy (DN) patients had lower serum Klotho levels.

Conclusion: The diagnosis of DN with serum klotho can be promising, and lower serum klotho serves as a good biomarker.

Keywords: Diabetic nephropathy (DN), eGFR, Soluble alpha-Klotho, Albuminuria, Type 2 diabetes mellitus


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References


Sadiq C, Hussein R, Maulood I. Ghrelin and Leptin and their Relations with Insulin Resistance in Diabetes Mellitus Type 2 Patients. Baghdad Science Journal, (2022); 19(1): 1-33.

Fadhel A, Al-Tameemi M, Alfarhani B. Biochemical Investigation in Blood Serum of Female Patients in Type-2 Diabetes. Journal of Global Pharma Technology, (2018); 10(10): 369-373.‏

Mustafa S, Hasan B, Ibrahim N. ESTIMATION OF FERRITIN. ERYTHROPOIETIN IN OBESE IRAQI TYPE II DIABETIC PATIENTS. Biochemical and Cellular Archives, (2019); 19(2): 3307-3312.‏

Ali K and Shaban S. Relation between Serum Leptin, Lipid Profiles and other biomarkers levels in patients with type 2 diabetic nephropathy. Baghdad Science Journal, (2010); 7(1): 678–686.

Alwan L, Khaleel F, Hameed A, Al-Ghani R. Determination of Polymorphism Of Glutathione S Transferase (GST) In The Iraqi (Diabetic and Non-Diabetic) Acromegalic Patients, Biochemical & Cellular Archives, (2020); 20(2): 000-000.

Guo J, Zheng H, Zhang W, Lou W, Xia C, et al. Accelerated kidney aging in diabetes mellitus. Oxidative Medicine and Cellular, (2020); 24(1): 000-000.

Fineberg D, Jandeleit K, Cooper M. Diabetic nephropathy: diagnosis and treatment. Nature Reviews Endocrinology, (2013); 9(12): 713-723.‏

Kuro O. Mutation of the mouse klotho gene leads to a syndrome resembling aging. Nature, (1997); 390(6659): 45-51.

Xu Y, & Sun Z. Molecular basis of Klotho: from gene to function in aging. Endocrine reviews, (2015); 36(2): 174-193.‏

Dalton G, Xie J, An S, Huang C. New insights into the mechanism of action of soluble klotho. Frontiers in endocrinology, (2017); 8(2017): 323.‏

Kuro M. The Klotho proteins in health and disease. Nature Reviews Nephrology, (2019); 15(1): 27-44.‏

Kim H, Nam B, Kim D, Kang M, Han J, et al. Circulating $alpha$-klotho levels in CKD and relationship to progression. American Journal of Kidney Diseases, (2013); 61(6): 899-909.‏

Typiak M, Piwkowska A. Antiinflammatory actions of klotho: implications for therapy of diabetic nephropathy. International Journal of Molecular Sciences, (2021); 22(2): 000-956.‏

Tziomalos K, Athyros V. Diabetic nephropathy: new risk factors and improvements in diagnosis. The review of diabetic studies: RDS, (2015); 12(12): 000-110.‏

Sueud T, Hadi NR, Abdulameer R, Jamil DA, Al-Aubaidy HA. Assessing urinary levels of IL-18, NGAL and albumin creatinine ratio in patients with diabetic nephropathy. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, (2019); 13(1): 0-56.

Morton JI, Liew D, McDonald SP, Shaw JE, Magliano DJ. The association between age of onset of type 2 diabetes and the long-term risk of end-stage kidney disease: a national registry study. Diabetes Care, (2020); 43(8): 1788-1795.

Kundu D, Roy A, Mandal T, Bandyopadhyay U, Ghosh E, et al. Relation of microalbuminuria to glycosylated hemoglobin and duration of type 2 diabetes. Nigerian journal of clinical practice, (2013); 16(2): 216-220.

Bhaisare SD, Rao AK, Jog AS, Kolapkar HU. Clinical Study of Urine Albumin Creatinine Ratio as an Earlier Predictor of Diabetic Nephropathy. Journal of Evolution of Medical and Dental Sciences, (2020); 9(9): 598-603.

Dokumacıoğlu E, Iskender H. Klotho Protein and Type 2 Diabetes Mellitus. Journal of Apitherapy and Nature, (2022); 5(2): 133-146.




DOI: http://dx.doi.org/10.62940/als.v12i3.1997

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