Biological Response and Quantitative Analysis of Bioactive Compounds in Rosmarinus officinalis Following Kinetin and Nano-Potassium Foliar Application

Zainab Abdel Rahim Najm, Omar Hazem Al-Rawi, Mustafa R Al-Shaheen

Abstract


Background: The study focuses on the biological response and estimation of active substances in Rosmarinus officinalis as a result of spraying with kinetin and Nanopotassium. The importance of Rosmarinus officinalis in both medicinal and nutritional contexts make it an ideal subject for testing the effectiveness of nanopotassium and kinetin, two compounds that have shown promise in enhancing plant growth and the development of bioactive compounds.

Methods: A field experiment was conducted in the Al-Boufraj area, Anbar Governorate, during the 2022-2023 season. The experiment involved two factors: nanopotassium at four levels (0, 20, 30, and 40 mg L⁻¹) and kinetin at three concentrations (0, 75, and 150 mg L⁻¹). The effects of these treatments on plant height, number of branches, stem diameter, chlorophyll content, and nitrogen content were analyzed using a randomized complete block design with three replications.

Results: The highest plant height (82.40 cm) and number of branches (11.33 branches plant⁻¹) were recorded at the highest levels of both nanopotassium and kinetin (40 mg L⁻¹ and 150 mg L⁻¹, respectively). The maximum stem diameter (6.10 mm) was obtained for the interaction between 30 mg L⁻¹ nanopotassium and 150 mg L⁻¹ kinetin (K30 × C150), while the main effect of kinetin at 150 mg L⁻¹ produced an average diameter of 5.47 mm. The maximum chlorophyll content (54.10 SPAD units) and nitrogen concentration (1.5185%) were also achieved when combining the highest levels of both treatments. Significant differences were observed across all main effects, while the interaction between nanopotassium and kinetin was significant for plant height, chlorophyll content, and nitrogen percentage, but not for branching and stem diameter.

Conclusion: The study demonstrates the positive impact of kinetin and nanopotassium on the growth characteristics and active compound production in Rosmarinus officinalis. The use of these substances, particularly in combination, offers a promising approach to improving the agricultural yield and quality of medicinal plants, with potential applications in sustainable farming practices.

Keywords: Rosmarinus officinalis, Kinetin, Bioactive compounds, Nanofertilizers, Nano potassium


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DOI: http://dx.doi.org/10.62940/als.v12i3.2805

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