Effect of Ruellia tuberosa L. Leaves Extract on SGOT and SGPT Levels and Liver Histopathology in Alloxan-Induced Diabetes White Rats (Rattus norvegicus)
Abstract
Background: Diabetes mellitus poses significant health risks, including liver damage, often caused by side effects of drug therapy. Ruellia tuberosa L. leaves, known for its antioxidant and antidiabetic properties, offers a promising natural alternative. This study evaluates its hepatoprotective potential by assessing Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels, along with liver histopathology in alloxan-induced diabetes white rats.
Methods: The research method used was a randomized posttest-only control group design. Alloxan was injected intraperitoneally with 150 mg/kg BW (single dose). Twenty-five male white rats were randomly divided into five groups: control group of diabetic white rats (K-), diabetic white rats given metformin (standard drug) dose of 50 mg/kg BW (K+), normal white rat control (P0), diabetic white rats given R. tuberosa leaves extract dose of 200 mg/kg BW (P1), and 400 mg/kg BW (P2). After 14 days, blood samples were analyzed for SGOT and SGPT levels, and liver tissues were examined histologically.
Results: The analysis showed that the administration of R. tuberosa leaves extract doses of 200 and 400 mg/kg BW in diabetic rats for 14 days had an improvement effect on SGOT, SGPT levels and histopathological images of liver organs.
Conclusion: R. tuberosa leaves extract, especially at the 400 mg/kg BW dose, can effectively lower SGOT and SGPT levels, also improving liver histopathology in diabetic rats. These findings suggest its potential as a complementary therapeutic option for liver complications in diabetic cases.
Keywords: Alloxan, Diabetes, Liver histopathology, SGOT, SGPT, Ruellia tuberosa L.
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Schleicher E, Gerdes C, Petersmann A, Müller-Wieland D, Müller UA, Freckmann G, et al. Definition, classification and diagnosis of diabetes mellitus 1. Experimental and Clinical Endocrinology & Diabetes, (2022); 130(S01): S1–S8.
Luo Z, Fu C, Li T, Gao Q, Miao D, Xu J, et al. Hypoglycemic effects of licochalcone A on the streptozotocin-induced diabetic mice and its mechanism study. Journal of Agricultural and Food Chemistry, (2021); 69(8): 2444–2456.
Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Research and Clinical Practice, (2019); 157: 107843.
Liu J, Ren ZH, Qiang H, Wu J, Shen M, Zhang L, et al. Trends in the incidence of diabetes mellitus: results from the global burden of disease study 2017 and implications for diabetes mellitus prevention. BMC Public Health, (2020); 20(1): 1–12.
Pansera K, Upasani C, Upangalwar A, Sonawane G, Patil C. Streptozotocin and alloxan induced diabetic nephropathy: protective role of natural products. Journal of the Maharaja Sayajirao University of Baroda, (2021); 55(1): 86–102.
Veeranjaneyulu C, Subrahmanyam G. Rediscovered the induction of diabetogenic agents in the experimental animal model: review. International Journal of Applied Biology and Pharmaceutical Technology, (2016); 7(3): 95–104.
Maqbool M, Dar MA, Gani I, Mir SA. Animal models in diabetes mellitus: An overview. Journal of Drug Delivery and Therapeutics, (2019); 9(1-s): 472–475.
Sheriff OL, Olayemi O, Taofeeq AO, Riskat KE, Ojochebo DE, Ibukunoluwa AO. A new model for alloxan-induced diabetes mellitus in rats. Journal of Bangladesh Society of Physiologist, (2020); 14(2): 56–62.
Martín-Carro B, Donate-Correa J, Fernández-Villabrille S, Martín-Vírgala J, Panizo S, et al. Experimental models to study diabetes mellitus and its complications: limitations and new opportunities. International Journal of Molecular Sciences, (2023); 24(12): 10309.
Li W, Lin K, Zhou M, Xiong Q, Li C, Ru Q. Polysaccharides from Opuntia milpa alta alleviate alloxan-induced INS-1 cells apoptosis via reducing oxidative stress and upregulating Nrf2 expression. Nutrition Research, (2020); 77: 108–118.
Solikhah TI, Solikhah GP. Effect of Muntingia calabura L. leaf extract on blood glucose levels and body weight of alloxan-induced diabetic mice. Pharmacognosy Journal, (2021); 13(6): 1450–1455.
Dra LA, Sellami S, Rais H, Aziz F, Aghraz A, Bekkouche K, et al. Antidiabetic potential of Caralluma europaea against alloxan-induced diabetes in mice. Saudi Journal of Biological Sciences, (2019); 26(6): 1171–1178.
Pansare K, Sonawane G, Mahajan T, Khairnar S, Patil C, Andhale A. Protective role of natural products in alloxan and streptozotocin induced diabetic nephropathy: A concise review. Natural Volatiles Essential Oils, (2021); 8(6): 3977–3994.
Feng J, Wang X, Xiaochun Ye, Ares I, Lopez-Torres B, Martínez M, et al. Mitochondria as an important target of metformin: The mechanism of action, toxic and side effects, and new therapeutic applications. Pharmacological Research, (2022); 177: 106114.
Rahman MM, Islam MR, Shohag S, Hossain ME, Rahaman MS, Islam F, et al. The multifunctional role of herbal products in the management of diabetes and obesity: A comprehensive review. Molecules, (2022); 27(5): 1713.
Alam S, Sarker MMR, Sultana TN, Chowdhury MNR, Rashid MA, Chaity NI, et al. Antidiabetic phytochemicals from medicinal plants: prospective candidates for new drug discovery and development. Frontiers in Endocrinology, (2022); 13: 800714.
Safitri A, Fatchiyah F, Sari DRT, Roosdiana A. Phytochemical screening, in vitro antioxidant activity, and in silico anti-diabetic activity of aqueous extracts of Ruellia tuberosa L. Journal of Applied Pharmaceutical Science, (2020); 10(3): 101–108.
Xu JH, Lo YM, Chang WC, Huang DW, Wu JSB, Jhang YY, et al. Identification of bioactive components from Ruellia tuberosa L. on improving glucose uptake in TNF‐α‐induced insulin‐resistant mouse FL83B hepatocytes. Evidence-Based Complementary and Alternative Medicine, (2020); 2020(1): 6644253.
Guha S, Talukdar D, Mandal GK, Mukherjee R, Ghosh S, Naskar R, et al. Crude extract of Ruellia tuberosa L. flower induces intracellular ROS, promotes DNA damage and apoptosis in triple negative breast cancer cells. Journal of Ethnopharmacology, (2024); 332: 118389.
Ramadhan M, Sabarudin A, Safitri A. In vitro anti-microbial activity of hydroethanolic extracts of Ruellia tuberosa L.: eco-friendly based-product against selected pathogenic bacteria. IOP Conference Series: Earth and Environmental Science, (2019); 239: 012028.
Dutta S, Hazra K, Ghosal S, Paria D, Hazra J, Rao MM. Morpho-anatomical and phytochemical characterisation of traditionally used plant Ruellia tuberosa L. leaves and roots. International Journal of Pharmacognosy, (2020); 7(1): 12–22.
Solikhah TI, Setiawan B, Ismukada DR. Antidiabetic activity of papaya leaf extract (Carica papaya L.) isolated with maceration method in alloxan-induced diabetic mice. Systematic Reviews in Pharmacy, (2020); 11(9): 774–778.
Ladeska V, Anggia V, Priyanto, Mentari I, Inas U, Permatasari DL, et al. Phytochemical, antioxidant studies of Ruellia tuberosa Linn, heart, and lung organs. Arabian Journal of Medicinal & Aromatic Plants, (2023); 9(3): 74–90.
Solikhah TI, Miftakhurrozaq RK, Khairullah AR. Antidiabetic activity and pancreatic histopathology analysis of Averrhoa bilimbi L. leaves extract on alloxan-induced diabetes on wistar mice. Advancements in Life Sciences, (2024); 11(3): 669–673.
Solikhah TI, Rani CA, Septiani M, Putra YA, Rachmah Q, Solikhah GP. Antidiabetic of Hylocereus polyrhizus peel ethanolic extract on alloxan induced diabetic mice. Iraqi Journal of Veterinary Sciences, (2022); 36(3): 797–802.
Khalaf SJ, Aljader GH, Sarhat ER, Sarhat TR, Abass KS. Antidiabetic effect of aqueous extract of Medicago Sativa with enhanced histopathology of pancreas in alloxan induced diabetic rats. Pakistan Journal of Medical and Health Sciences, (2021); 15(2): 492–496.
Solikhah TI, Wijaya TA, Pavita DA, Kusnandar R, Wijaya A, Raharjo HM, et al. The effect of sapodilla leaf extract (Manilkara zapota L.) on lipid profiles of alloxan-induced diabetic mice. Pharmacognosy Journal, (2023); 15(2): 286–289.
Bari MW, Islam MM, Khatun M, Sultana MJ, Ahmed R, Islam A, et al. Antidiabetic effect of Wedelia chinensis leaf extract in alloxan induced Swiss albino diabetic mice. Clinical Phytoscience, (2020); 6: 58.
Longkumer S, Jamir A, Kechu M, Ezung S, Prankaj PP. Alloxan monohydrate induced diabetes: A comprehensive review. International Journal of Innovative Life Sciences, (2021); 1(1): 6–14.
Suputri NKAW, Azmijah A, Bijanti R, Putra MM. Effects of onion extract on hepar histopathology in alloxan-induced diabetic Rattus novergicus. Medico-Legal Update, (2020); 20(3): 384–390.
Pham TNT, Nguyen TT, Nguyen TLT, Tran AMN, Nguyen TN, Tong DT, et al. Antioxidant and anti-inflammatory activities of phytochemicals from Ruellia tuberosa. Journal of Chemistry, (2022); 2022: 1–14.
Cozma GV, Apostu A, Macasoi I, Dehelean CA, Cretu OM, Dinu S, et al. In vitro and in ovo evaluation of the potential hepatoprotective effect of metformin. Medicina, (2022); 58(6): 705.
Iranshahy M, Rezaee R, Karimi G. Hepatoprotective activity of metformin: A new mission for an old drug?. European Journal of Pharmacology, (2019); 850: 1–7.
DOI: http://dx.doi.org/10.62940/als.v12i3.3647
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