Dissemination of Bt cotton in cotton growing belt of Pakistan

Saira Azam, Tahir Rehman Samiullah, Aneela Yasmeen, Salah ud Din, Adnan Iqbal, Abdul Qayyum Rao, Idrees Ahmad Nasir, Bushra Rashid, Ahmad Ali Shahid, Munir Ahmad, Tayyab Husnain



Background: Adoptability of biotech crops has climbed up dramatically in the world from 1.7 million hectares in its introductory period in 1996 to 170 million hectare in 2012. Area planted to biotech crop increased to over one-fourth of the world total in 2005-06. The data collected also demonstrates that developing countries like Pakistan are making tremendous development in production of Biotech crops especially cotton.

Methodology: Random samples have been collected and received from different cotton growing areas of Pakistan from 2007 to date for Bt gene confirmation and expression. Genomic DNA was isolated and analyzed through internal reference primers from SadI gene of cotton genome. Samples were analyzed by PCR for detection of Bt genes including CEMB Cry1Ac+2A double Bt gene. ELISA was done for confirmation of Bt protein by using Envirologix Quantiplate ELISA kit Cry1Ab/Ac Cat # AP003 and Quantiplate ELISA kit Cry2A Cat # AP005 according to manufacturer instruction.

Results: Development of Bt Cotton in Pakistan is as old as in advanced countries of the world. Pakistan has become the fourth country of the world for production of indigenous Bt cotton by utilizing their local cotton varieties for genetic modifications. The support of various documentary proofs like research articles, patents, projects, Ph. D and M.Phil studies generated by Centre of Excellence in Molecular Biology (CEMB) greatly strengthen the data of Bt cotton development in Pakistan. Pakistan Central Cotton Committee (PCCC)declared locally developed Bt cotton as the best performing of all over Pakistan among other international product.

Conclusion: Progress made in development of indigenous Bt cotton variety by CEMB Pakistan and their excellent performance in field determined their increased adoptability ratio in farmers. The formal approval process which is going to be completed in near future will open the doors for farmers and breeders to utilize this material for better economy of Pakistan.

Full Text:



Bakhsh A, Rao AQ, Shahid AA, Husnain T, Riazuddin S. Insect resistance and risk assessment studies in advance lines of Bt cotton harboring Cry1Ac and Cry2A genes. American-Eurasian Journal of Agriculture and Environmental Science, (2009); 6(1): 1-11.

Khan AI, Fu Y-B, Khan IA. Genetic diversity of Pakistani cotton cultivars as revealed by simple sequence repeat markers. Communications in Biometry and Crop Science, (2009); 4(1): 21-30.

Chapagain A, Hoekstra A, Savenije H, Gautam R. The water footprint of cotton consumption: An assessment of the impact of worldwide consumption of cotton products on the water resources in the cotton producing countries. Ecological Economics, (2006); 60(1): 186-203.

Sabir HM, Tahir SH, Khan MB. BT Cotton and its Impact on Cropping Pattern in Punjab. Pakistan Journal of Social Sciences (PJSS), (2011); 31(1): 127-134.

Rashid B, Saleem Z, Husnain T, Riazuddin S. Transformation and inheritance of Bt genes inGossypium hirsutum. Journal of Plant Biology, (2008); 51(4): 248-254.

Majeed A, Makhdoom R, Husnain T, Riazuddin S. Assessment of potato proteinase inhibitor-II gene as an antifungal and insecticidal agent. Acta Agriculturae Scandinavica Section B–Soil and Plant Science, (2011); 61(1): 92-96.

Bashir K, Husnain T, Fatima T, Latif Z, Mehdi SA, Riazuddin S. Field evaluation and risk assessment of transgenic indica basmati rice. Molecular Breeding, (2004); 13(4): 301-312.

Rao AQ, Bajwa KS, Puspito AN, Khan M, Abbas MA, Rehman M, Baksh A, Shahid AA, Nasir IA, Husnain T. Variation in Expression of Phytochrome B Gene in Cotton (Gossypium hirsutum L.). Journal of Agricultural Science and Technology, (2013); 15(5): 1033-1042.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, (1976); 72(1): 248-254.

Kiani S, Mohamed BB, Jamal A, Shahid MN, Shehzad K, et al. Chloroplast-targeted Expression of Recombinant Crystal-protein Gene in Cotton: An Unconventional Combat with Resistant Pests. Journal of Biotechnology, (2013).

Rao AQ, Bakhsh A, Kiani S, Shahzad K, Shahid AA, et al. The myth of plant transformation. Biotechnology advances, (2009); 27(6): 753-763.

Rao AQ, Irfan M, Saleem Z, Nasir IA, Riazuddin S, et al. Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum). Journal of Zhejiang University SCIENCE B, (2011); 12(4): 326-334.

Riaz N, Husnain T, Fatima T, Makhdoom R, Bashir K, et al. Development of Indica Basmati rice harboring two insecticidal genes for sustainable resistance against lepidopteran insects. South African Journal of Botany, (2006); 72(2): 217-223.

Maqbool SB, Riazuddin S, Loc NT, Gatehouse AM, Gatehouse JA, et al. Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Molecular Breeding, (2001); 7(1): 85-93.

Majeed A, Husnain T, Riazuddin S. Transformation of Virus-Resistant Genotype of Gossypium hirsutum L. with Pesticidal Gene. Plant Biotechnology, (2000); 17(2): 105-110.

Husnain T, Asad J, Maqbool SB, Datta SK, Riazuddin S. Variability in expression of insecticidal Cry1Ab gene in Indica Basmati rice. Euphytica, (2002); 128(1): 121-128.

Zia-ur-Rehman I, Zafar AU, Nasir IA, Riazuddin S. Comparative study of Bacillus thuringiensis biopesticides against cotton bollworms. Asian Journal of Plant Sciences, (2002); 1(5): 574-576.

Bakhsh A, Rao AQ, Shahid AA, Husnain T, Riazuddin S. CaMV 35S is a developmental promoter being temporal and spatial in expression pattern of insecticidal genes (cry1ac & cry2a) in cotton. Austerlain Journal of Basic and Applied Sciences, (2010); 4(1): 37-44.

Bakhsh A, Rao AQ, Shahid AA, Husnain T. Spatio Temporal Expression Pattern of an Insecticidal Gene (cry2A) in Transgenic Cotton Lines. Notulae Scientia Biologicae, (2012); 4(4): 115-119.


  • There are currently no refbacks.