In vitro biochemical evaluation of methanol extract of Moringa oleifera pods on rat liver mitochondrial membrane permeability transition pore and lipid peroxidation

Elohor Cassandra Bezi, Oluwaseyi Adegoke Adetunji, Olaoluwa Temitope Talabi


Background: Moringa oleifera is a well-known world herbal plant for its amazing medicinal and nutritional properties. The effect of methanol extract of M. oleifera can be useful in managing diseases associated with mitochondrial membrane permeability transition pore and lipid peroxidation.

Methods: Evaluation was done at varying concentrations of the methanol pods extract on mitochondrial membrane permeability transition pore opening (swelling) and Fe2+- H2O2-EDTA (Fenton reaction)-induced lipid peroxidation in vitro. Five male albino rats (weighed 120-250 g) were anaesthetized and sacrificed; the liver was excised and homogenized to obtain mitochondria pellets. This study analyzed the effect of varying concentrations of methanol pods extract of M. oleifera at 50, 150, and, 300 µg/ml respectively. The effects of M. oleifera varying concentration in vitro was determined using malondialdehyde reaction quantified at 532 nm in a UV- spectrophotometer as index for lipid peroxidation and spectrophotometric absorptions at 520 nm was observed as an index of  mitochondrial membrane permeability pore respectively.

Results: Varying concentrations of methanol pods extract of M. oleifera at 50, 150, and 300 µg/ml in the presence and absence of triggering agent (Ca2+) inhibited opening of mitochondria membrane permeability transition pore while 0.25, 0.50 and 1.00 mg/ml inhibited lipid peroxidation induced mitochondria of the rat liver respectively in a concentration dependent mode.

Conclusion: The results suggest that methanol extract of M. oleifera pods at high concentrations (such as 300 µg/ml and 1.00 mg/ml respectively) may inhibit mitochondrial membrane permeability transition pore opening and lipid peroxidation. 

Keywords: Moringa oleifera, mitochondria membrane permeability transition pore, lipid peroxidation

Full Text:



Croteau R, Kutchan TM, Lewis NG. Natural products (secondary metabolites). In: B. Buchanan, W. Gruissem, R. Jones, (Eds.), Biochemistry and molecular biology of plants. Rockville, MD: American Society of Plant Physiology, (2000); 1250-1318.

Devendra BN, Shrinivas N, Prasad TV, Swarna L. Antimicrobial activity of Moringa oleifera leaf extract, against selected bacterial and fungal strains. International Journal of Pharma Bioscience and Technology, (2011); 2(3): 13-18.

Patil SD, Jane R. Antimicrobial activity of Moringa oleifera and its synergism with Cleome viscose. International Journal of Life Sciences, (2013); 1(3):182-189.

Sharma V, Paliwal R. Isolation and characterization of saponins from Moringa oleifera (moringaeceae) pods. International Journal of Pharmacy and Pharmaceutical Sciences, (2013); 5(1), 179-183.

Paliwal R, Sharma V, Pracheta S, Yadav S, Sharma S. Anti-nephrotoxic effect of administration of Moringa oleifera Lam in amelioration of DMBA-induced renal carcinogenesis in Swiss albino mice. Biology and Medicine, (2011); 3(2): 27-35.

Pal SK, Mukherjee PK, Saha BP. Studies on the antiulcer activity of M. oleifera leaf extract on gastric ulcer models in rats. Phytotherapy Research, (1995); 9: 463-465.

Crompton M, Barksby E, Johnson N, Capano M. Mitochondrial intermembrane junctional complexes and their involvement in cell death. Biochimie, (2002); 84: 143–152.

Duchen MR, Mitochondria in health and disease: perspectives on a new mitochondrial biology. Molecular Aspects of Medicine, (2004); 25(4): 365–451.

Petronilli V, Penzo D, Scorrano L, Bernardi P, Lisa F. The mitochondrial permeability transition, release of cytochrome c and cell death: correlation with the duration of pore openings in situ. Journal of Biological Chemistry, (2001); 276: 12030–12034.

Petit PX, Goubern M, Diolez P, Susin SA, Zamzami N, Kroemer G. Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: the impact of irreversible permeability transition. FEBS Letters, (1998); 426:111 –116.

Lardy HA, Wellman H. The catalytic effect of 2,4 dinitrophenol on adenosine triphosphate hydrolysis by cell particles and soluble enzymes. Journal of Biological Chemistry, (1953); 201(1): 357-370.

Lapidus RG, Sokolove P M. Spermine inhibition of permeability transition of isolated rat liver mitochondria: an investigation mechanism. Archive of Biochemistry and Biophysics, (1993); 306(1): 246-253.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Annual Review of Biochemistry, (1979); 95: 351-8.

Arabshahi DS, Devi V, Urooj A. Evaluation of antioxidant activity of some plant extracts and their heat, pH and storage stability. Food Chemistry, (2007); 100: 1100–1105.

Kumar A, Pari L. Antioxidant action of Moringa oleifera Lam (drumstick) against antitubercular drugs induced lipid peroxidation in rats. Journal of Medicinal Food, (2003); 6: 255–259.

Bharali R, Tabassum J, Azad MR. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pacific Journal of Cancer Prevention, (2003); 4: 131–139.