First record of Cantharellus minor from Vietnam with identification support from a combination of nrLSU and nrSSU phylogenetic analysis

Thuan Duc Lao, Nghia Trong K, Tai Van Ngo, Nguyen Binh Truong, Luyen Tien Vu, Thuy Ai Huyen Le

Abstract


Background: A previously identified sample XC02, which was collected from a pine forest (Pinus kesiya Royle ex Gordon), in Xuan Tho Commune, Da Lat, Lam Dong Province, Vietnam, was identified as Cantharellus minor based on morphology and nrLSU phylogeny analysis. Sequence analysis of multiple genes are becoming more and more common for phylogenetic analysis of mushrooms.

Method: Total DNA was isolated from sample XC02. The primer NS1, NS4 were applied to amplify the target gene the nuclear ribosomal small subunit DNA (nrSSU). For phylogenetic analysis, individual and concatenated datasets (nrSSU and nrLSU-nrSSU) were constructed. Phylogenetic tree was constructed with MEGA 6.0 with a 1000 replicate bootstrap based on the neighbor joining, maximum likelihood, maximum parsimony method.  

Results: A concatenated dataset containing a total of 14 sequences from Cantharellus, Craterellus (Cantharellaceae, Canthraellales) and Hydnum (Hydnaceae, Cantharellales) were constructed. For the specimen XC02, the phylogenies based on the first, second, and third datasets (nrLSU, nrSSU, and nrLSU-nrSSU) and the morphological analysis, reported in our previous study, strongly confirmed the identity of XC02 as Cantharellus minor.

Conclusion: The combination between the morphological analysis and phylogenetic analysis is confirmed as the best approach for the identification of Cantharellus and other mushroom species that we collected in the Central Highlands, Vietnam.


Keywords: nrLSU; Cantharellus, Cantharellus minor; nrSSU; nrLSU; phylogeny analysis; Vietnam


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References


Peck CH. Report of the Botanist (1869). Annual Report on the New York State Museum of Natural History. (1873); 23:27-135.

Deepika K, Reddy MS, Upadhyay RC. New records of Cantharellus species from the northwestern Himalayas of India. Mycology. (2013); 4(4):205-220.

Phan HH, Lao DT, Le HAT, Hoang QK, Truong BN, Ngo TLG. First record of Cantharellus minorin Vietnam. Journal of Biotechnology, (2017); 15(4): 669-673.

Dahlman M, Danell E, Spatafora JW. Molecular systematics of Craterellus: cladistic analysis of nuclear LSU rDNA sequence data. Mycological research, (2000); 104(4): 388-394.

Moncalvo JM, Nilsson RH, Koster B, Dunham SM, Bernauer T, Matheny PB, et al. The Cantharelloid clade: dealing with incongruent gene trees and phylogenetic reconstruction methods. Mycologia, (2006); 98(6): 937-948.

Tibuhwa DD, Saviæ S, Tibell L, Kivaisi AK. Afrocantharellus gen. stat. nov. is part of a rich diversity of African Cantharellaceae. IMA fungus, (2012); 3(1): 25-38.

Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology, (1990); 172(8): 4238-4246.

Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nature methods, (2012); 9(8): 772.

Nguyen PDN, Tran DK. Impacts of ecological factors on the distribution of Amauroderma murrill genus in Central Highlands of Vietnam. Journal of Scientific and Engineering Research, (2017); 4(9): 238-243.

Kuo M. Cantharellus minor. MushroomExpert.Com. (2006) Retrieved 2011-03-24.

Deepika K, Reddy MS, Upadhyay RC. New records of Cantharellus species from the Northwestern Himalayas of India. Mycology, (2014); 4(4): 205-220.

Kumar S, Nei M, Dudley J, Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in bioinformatics, (2008); 9(4): 299-306.


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