Effect of camel milk lactoferrin against carbon tetrachloride induced hepatic toxicity in Sprague Dawley rats
Abstract
Background:Chronic Hepatitis and mortality due to liver cirrhosis is common in Pakistan. Camel milk Lactoferrin has antiviral, anti-inflammatory properties. Nutraceutical foods like camel milk have many uses. Camel milk lactoferrin might be used for the cure of hepatic fibrosis induced by CCI4 in Sprague Dawley rats.
Methods: Seventy-five (75) male Sprague–Dawley rats were purchased from National Institute of Health Islamabad kept in animal house of UVAS Lahore and randomly divided into 5 groups under completely randomize design. In Each group carbon tetrachloride (CC14) was subcutaneously injected with a mixture of 40% CCI4 (a mixture of pure CC14 and sterile olive oil) at 200 uL/100g body weight as single dose, 48 hrs before the starting of treatment or 0 day. After 48 hrs. Rats were considered hepatic injured except the (+ve) control group. Among all groups, four groups –ve control, 30mg/kg/b.wt, 60mg/kg/b.wt and 90mg/kg/b.wt were supplied with standard diet plus Lactoferrin (different concentrations and doses) orally, while control group were provided only standard diet throughout the efficacy study (30 days).
Results: The study was conducted in two phases, first one was on the isolation and purification of camel milk lactoferrin and the second one was based on efficacy study of lactoferrin in Sprague Dawley rats against the hepatic toxicity induced by carbon tetrachloride
Conclusion: The present study on CCl4 induced hepatotoxicity in Sprague Dawley rats clarifies that camel milk lactoferrin induced significant improvement in serum level of ALP, AST, AST, bilirubin, serum urea and serum Creatinine within the duration of 4 weeks treatment.
Keywords: Carbon tetrachloride; Complete randomized design; Aspartate aminotransferase
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