Detection of Human Salivary Amylase Level Deposited on Fruits with First Bite Mark

Umar Draz, Saadat Ali, Sehrish Firyal, Ahmad Ali, Ali Haider Saleem, Mohammad Ashraf Tahir


Background: Human body fluids are vital for criminological examinations. The recovery, conservation, and investigation of body fluids or its stains are critical in measurable forensic cases. The amylase testing is being utilized as a possible test to locate the crime scene saliva stains for more than three decades. This investigation was intended to decide the human salivary amylase accumulated on fruit after the first bite where the level of salivary amylase was concluded by Radial Gel Diffusion method.

Methods: Three sorts of organic fruits (apple, peach, and apricot) and 20 people (10 male and 10 female) were chosen for this investigation. The specimens were assembled from bite mark area following time intervals 0 hours, 12 hours, 24 hours, 36 hours and 48 hours.

Results: Experimental outcomes demonstrated that the diameter of amylase activity in male specimens was more noteworthy than female. Apples, as well as apricot pit samples, indicated amylase activity till 48 hours; however, the specimens collected from peach demonstrated amylase activity until 12 hours, after first bite mark.

Conclusion: This study demonstrated that salivary amylase testing might be valuable for DNA evidence, where DNA investigations for every case might be restricted because of cost issues.

Keywords: Amylase; Saliva; Investigation; Bite mark; Preservation

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