Single nucleotide polymorphism and phylogenetic analysis of the exon 2 of leptin gene in Lohi sheep

Ali Haider Saleem, Ahmad Ali, Akhtar Rasool Asif, Asad Ali, Hamid Mustafa, Dilshad Rashid, Mujahid Zafar, Muhammad Awais, Hafiz Ishfaq Ahmad, Zulqarnain Baqar

Abstract


Background: Leptin hormone, encoded by leptin (LEP) gene is involved in many biological and physiological processes in the body. Polymorphism in LEP gene has been observed and correlated with a variety of reproductive and productive traits in several sheep breeds worldwide, but its role has not been much studied in local sheep breeds of Pakistan. The present study was conducted to analyze polymorphism in LEP gene in Lohi breed of sheep.

Methods: Subsequent to statistical analysis (generalized linear model), 18 animals were selected randomly from the flock for blood samples collection followed by DNA extraction, amplification using PCR prior to sequencing. The amplified product of exon 2 and partial intron 2 regions of LEP gene was 268bp.

Results: Molecular analysis showed a heterozygous condition i.e. C>Y at position 15 and 18 in exon 2. The data on average daily weight gain (ADG) from birthday to 90 days were used for association study, while environmental effects were minimized by means of generalized linear model. Association of polymorphisms in LEP gene with ADG did not yield any significant results.

Conclusion: In conclusion, analysis of LEP gene sequence verified the existence of genetic changes in Lohi sheep. Further investigations are needed to find variations that might be linked with traits of economic importance for upcoming breeding program sand marker-assisted selection.

Keywords: DNA; Exon 2; LEP; PCR; Lohi


Full Text:

PDF

References


Ali A, Babar ME, Mahmood S, Imran M. First report of GTG-banded nomenclature of Pakistani Lohi sheep (Ovis aries). Turkish Journal of Veterinary and Animal Sciences, (2011); 35(4): 213-217.

Fiaz M, Munawar K, Mushtaq M, Ahmad M, Chaudhry MA, et al. Evaluating Varying Calf Milk Replacers for Optimum Growth Performance in Salt Range Lambs. Pakistan J Zool, (2017); 49(5): 1-4.

Babar M, Ahmad Z, Nadeem A, Yaqoob M. Environmental factors affecting birth weight in Lohi sheep. Pakistan Veterinary Journal, (2004); 24(1): 5-8.

Singh U, Kumar S, Ganguly I, Gaur G, Jagadeesan K, et al. Identification of genetic polymorphism in two exonic coding region of Leptin gene among indigenous and crossbred cattle. Indian Journal of Animal Research, (2014); 48(5): 403-407.

Meena A, Bhatt R, Sahoo A, Kumar S. Polymorphism of the exon 3 of leptin gene in Malpura sheep. Indian Journal of Animal Research, (2017); 51(3): 469-473.

Javanmard A, Mohammadabadi M, Zarrigabayi G, Gharahedaghi A, Nassiry M, et al. Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russian Journal of Genetics, (2008); 44(4): 495-497.

Priyadarshini L, Yadav AK, Singh HS, Mishra A, Jain AK, et al. Role of leptin in physiology of animal reproduction-A review. Agricultural Reviews, (2015); 36(3): 235-240.

Bakhtiar R, Abdolmohammadi A, Hajarian H, Nikousefat Z, Kalantar-Neyestanaki D. Identification of g. 170G> A and g. 332G> A mutations in exon 3 of leptin gene (Bcnl and Cail) and their association with semen quality and testicular dimensions in Sanjabi rams. Animal reproduction science, (2017); 179: 49-56.

Kaplan S, Atalay S. Single Nucleotide Polymorphism of Ovine Leptin and Insulin-Like Growth Factor 1 Gene in Kivircik Crossbred Ewes. Pakistan Journal of Zoology, (2018); 50(3): 851-856.

Babar ME (1994). GENETICS AND PHENOTYPIC PARAMETERS OF SOME PERFORMANCE CHARACTERISTICS OF LOHI SHEEP: PhD Thesis. University of Agriculture Faisalabad.

Babar M, Hussain T, Sadia H, Nadeem A, Kamran M, et al. Mitochondrial Cytochrome B gene based phylogeny of Lohi and Thalli sheep breeds of Pakistan. Journal of Animal and Plant Sciences, (2014); 24(4); 1260-1262.

Saleem AH, Javed K, Babar ME, Hussain T, Ali A, et al. Association of Leptin Gene Polymorphism with Growth Rate in Lohi Sheep. Pakistan Journal of Zoology, (2018); 50(3): 1029-1033.

Zhang B, Chu L, Liu H, Xie C, Qiao S, et al. Leucine Supplementation in a Chronically Protein-Restricted Diet Enhances Muscle Weight and Postprandial Protein Synthesis of Skeletal Muscle by Promoting the mTOR Pathway in Adult Rats. Engineering, (2017); 3(5): 760-765.

Iqbal Qureshi Z, Farid AH, Ellahi Babar M, Hussain T. Leptin Gene Polymorphism in Lohi, Kajli and Spili Breeds of Sheep. Pakistan Veterinary Journal, (2015); 35(2):321-324.

Jonas E, Martin G, Celi P, Li L, Soattin M, et al. Association of polymorphisms in leptin and leptin receptor genes with circulating leptin concentrations, production and efficiency traits in sheep. Small Ruminant Research, (2016); 136: 78-86.

Boucher D, Palin M, Castonguay F, Gariépy C, Pothier F. Detection of polymorphisms in the ovine leptin (LEP) gene: Association of a single nucleotide polymorphism with muscle growth and meat quality traits. Canadian journal of animal science, (2006); 86(1): 31-35.

Wang P, Deng L, Zhang B, Chu M, Tan Y, et al. Identification of polymorphism on leptin receptor gene of goats in southwest China. Small ruminant research, (2011); 96(2-3): 120-125.

Hajihosseinlo A, Hashemi A, Sadeghi S. Association between polymorphism in exon 3 of leptin gene and growth traits in the Makooei sheep of Iran. Livestock Research for Rural Development, (2012); 24(9): 543-546.

Singh S, Rout P, Agarwal R, Mandal A, Singh S, et al. Characterization of exon 2 and intron 2 of leptin gene in Indian goats. Animal biotechnology, (2009); 20(2): 80-85.

Maitra A, Sharma R, Pandey A, Singh L, Mandakmale S, et al. Preliminary identification and characterisation of leptin gene polymorphism in Indian goats. Journal of applied animal research, (2014); 42(1): 118-122.


Refbacks

  • There are currently no refbacks.