Prevalence of Colibacillosis in Young Broiler Chicks and Antibiogram of Escherichia coli in Different Areas of Hazara Region

Aysha Tahir, Muhammad Azam Khan, Kayanat Bibi, Sana Bibi, Fatima Rauf, Farwa Ayaz

Abstract


Background: Poultry sector is a vital zone of Pakistan economy and is a feasible source of animal derivative protein.  In term of mortality and morbidity the development of this segment is significantly affected by a lot of problems. Hazara region is well known for poultry in Pakistan. Colibacillosis, caused by Escherichia coli is vital disease among poultry of all ages resulting in huge economic losses. The aim of this study was to evaluate the prevalence of colibacillosis in young broiler chickens and antibiogram of E. coli in different areas of Hazara region.

Methods: The current study was conducted to evaluate the prevalence of colibacillosis in young broiler chickens and antibiogram of E. coli. This study was done during February 2019 to June 2019; a total of 200 (n=200) liver samples of freshly dead young broiler chicks were randomly collected from the postmortem section of Veterinary research and disease investigation center Abbottabad (VRDICA). The identification of E. coli was confirmed by gram staining and biochemical tests. Susceptibility pattern to 13 antibiotics was also checked.

Results: Total of 200 (n=200) samples were brought to the VRDICA for the diagnosis of disease and antibiotic susceptibility testing. Out of these 130 were positive (Overall prevalence =65%).Area wise prevalence varied from area to area and highest prevalence was recorded in Abbottabad (81.11%) followed by Havelian (75%), Manghal (72.22%), Mansehra (60%), Qalandarabad (50%) and Haripur (28.57%). About age group, highest mortality was found in 11-15 days old chicks (93%) as compared to 6-10days (83.33%) and 1-5 days old chicks (21.42%). Antibiogram showed highest sensitivity to Gentamicin (100%) afterward Colistin Sulphate (92.30%). However, lincomycin (92%) and streptomycin (96%) display highest resistance.

Conclusion:  In this study, the highest prevalence of infection was observed in young chicks that play a crucial role in their mortality. E. coli had showed resistance to commonly recommended antibiotic so care should be taken while prescribing the drugs.

Keywords: Young broiler chicks; E. coli; Colibacillosis; Prevalence; Antibiogram 


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References


Li G, Laturnus C, Ewers C, Wieler LH. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infection and immunity, (2005); 73(5): 2818-27.

Kabir SM. Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. International journal of environmental research and public health, (2010); 7(1): 89-114.

Barnes J, Nolan L, Valliancourt J. Colibacillosis: Diseases of Poultry. 2008; 3: 716-762. Saif Y.M. Iowa, Blackwell Publishing Professional.

Ahmed AM, Shimamoto T, Shimamoto T. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. International Journal of Medical Microbiology, (2013); 303(8): 475-483.

Singer RS, Hofacre CL. Potential impacts of antibiotic use in poultry production. Avian diseases, (2006); 50(2):161-172.

Amer MM, AM Amer, ER Hassan and AM Ghetas. Salmonella Enteritidis in broiler chickens: isolation, antibiotic resistance phenotyping and efficacy of colistin on control of experimental infection. International Journal of Veterinary Science, (2020); 9(2): 267-272.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, (1966); 45(4): 493-6.

Srinivasan P, Rao GS, George VT. Serotyping of Escherichia coli isolated from natural cases of colibacillosis in chicken in and around Namakkal. Indian Veterinary Journal, (2003); 41: 48-50.

Biswas PK, Uddin GM, Barua H, Roy K, Biswas D, Ahad A, Debnath NC. Causes of loss of Sonali chickens on smallholder households in Bangladesh. Preventive veterinary medicine, (2006); 76(3-4):185-95.

Tonu NS, Sufian MA, Sarker S, Kamal MM, Rahman MH, Hossain MM. Pathological study on colibacillosis in chickens and detection of Escherichia coli by PCR. Bangladesh Journal of Veterinary Medicine, (2011); 9(1):17-25.

Hashem M, Elahi M, Mannan M, Kabir M, Kashem M, Pallab M. Isolation, identification and antibiogram of Escherichia coli from broiler at Chittagong District in Bangladesh. Wayamba Journal of Animal Science, (2012); 6: 312-316.

Onderka DK, Hanson JA, McMillan KR, Allan B. Escherichia coli associated cellulitis in broilers: correlation with systemic infection and microscopic visceral lesions, and evaluation for skin trimming. Avian diseases, (1997); 41(4):935-40.

Khalifa E, Hamed NA, El-Mageed AE, El-Rahman A. Escherichia coli as a causative agent in omphalitis in broiler chicks. Animal Health Research Journal, (2013); 1(3):26-35.

Mehmood K, Bilal RM and Zhang H. Study on the genotypic and phenotypic resistance of tetracycline antibiotic in Escherichia coli strains isolated from free ranging chickens of Anhui Province, China. Agrobiological Records,( 2020); 2: 63-68.




DOI: http://dx.doi.org/10.62940/als.v8i3.837

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