Seroprevalence and Risk factor of Brucella Abortus in Cattle (Indigenous and crossbred) in District Layyah

Muhammad Ikram, Asghar Hussain, Muhammad Kashif, Muhammad Tarique Tunio, Usman Waheed, Asghar Abbas, Asghar Abbas, Tanveer Ahmad, Atif Rahman

Abstract


Background: Brucellosis is very important infectious, zoonotic disease all over the world affecting variety species, causing severe economic losses in animals and severe illness in human beings. This study was planned to find the seroprevalence and associated risk factors with Brucella Abortus in indigenous and crossbreed cattle of District Layyah.

Methods: The blood sample were collected from 350 blood animals and screened by Rose Bengal plate test (RBPT) using anti-Brucella antibodies. The seropositive samples were confirmed through indirect ELISA tests. The information on risk factors associated with brucellosis were collected on pre-designed questionnaires.

Results: The result of the present study showed the overall prevalence of 6.97% in cattle in district Layyah. It was more prevalent in the age group of 2-5 years (14.6%) as compared to that of the age group 5-10 years (6.25%). The prevalence was significantly higher in the cattle with history of abortion (52.38%) as compared to the non-aborted cattle (3%). The crossbreed animals were found to be more affected than indigenous animals.

Conclusion: There is a dire need to eliminate the seropositive animals by application of proper control programme and eradicate this zoonotic disease in developing countries especially in Pakistan.

Keywords: Brucellosis; Cattle; Seroprevalence; Layyah 


Full Text:

PDF

References


Anonymous. Economic Survey of Pakistan 2018-19 Chapter 2: Agriculture; Ministry of Finance: Islamabad, (2019); pp: 71-74.

Anonymous. Economic Survey of Pakistan 2016-17 Chapter 2: Agriculture; Ministry of Finance: Islamabad (2017); pp: 50-55.

Schelling E, Diguimbaye C, Daoud S, Nicolet J, Boerlin P, Zinsstag J. Brucellosis and Q-fever sero-prevalence of nomadic pastoralists and their livestock in Chad. Preventive Veterinary Medicine, (2003); 61: 279-293.

McDermott JJ, Arimi SM. Brucellosis in Sub-Saharan Africa: Epidemiology, control and impact. Veterinary Microbiology, (2002); 90: 111-134.

Farooq U, Fatima Z, Afzal, M, Anwar, Z, Jahangir M. Sero-prevalence of brucellosis in bovines at farms under different management conditions. Brazilian Journal Dairy Science, (2011); 2: 35-39.

Bricker BJ. PCR as a diagnostic tool for Brucellosis. Veterinary Microbiology, (2009); 90: 435-446.

Maadi HM, Moharamnejad H, Haghi M. Prevalence of Brucellosis in Cattle in Urmia, Iran. Pakistan Veterinary Journal, (2011); 31: 81-82.

Rahman MM, Haque M, Rahman MA. Sero-prevalence of caprine and human Brucellosis in some selected areas of Bangladesh. Bangladesh Veterinary Journal, (1988); 22: 85-92.

Refai M. Incidence and control of brucellosis in the Near East region. Veterinary Microbiology, (2002); 90: 81-110.

Cutler S, Whitmore A. Progress in understanding Brucellosis. Veterinary Research, (2013); 153: 641-642.

Ali S, Akther S, Neubauer H, Melzer F, Khan I, Qurban A, Irfan M. Serological, cultural, and molecular evidences of Brucella infection in small ruminants in Pakistan. Journal of Infectious diseases, (2015); 9: 470-475.

Shahzad A, Khan A, Khan MZ, Saqib M. Seroprevalence and molecular investigation of brucellosis in camels of selected districts of Punjab, Pakistan. Thai Journal of Veterinary Medicine, (2017); 47: 207-215.

Yilmaa M, Gezahegne M, Bedaso M. Review on brucellosis, sero-prevalence and ecology in livestock and human population of Ethiopia. Achievement in life sciences, (2016); 10: 80-86.

Raiesul-Islam M, Mohinder-Pratap G, Gursimran F, Pritam KS, Tawheed AS, Suhail AB, Sayed AH, Radya M. Sero-Epidemiology of brucellosis in organized cattle and buffaloes. Advances in Animal and Veterinary Sciences, (2013); 35: 550-557.

Shafee M, Rabbani M, Ahmad MUD, Muhammad K, Sheikh AA, Awan MA, Shabbir MZ. Seroprevalance of bovine brucellosis using indirect ELISA in Quetta, Balochistan, Pakistan. Journal Animal and Plant Sciences, (2012), 22: 125-127.

Zadon S, Sharma NS, Arora AK. Development of the novel loop mediated isothermal amplification (lamp) of is711 sequence for rapid detection of brucella species. India Journal Biological Sciences, (2015); 85: 685-691.

Zamin I, Isaac T, Gil M. High-throughput microbial population genomics using the Cortex variation assembler. Bioinformatics, (2013); 29: 275-276.

Zahid I, Huma J, Zafar IQ, Saqib M, Laeeq AL, Salman MW, Safdar M. Seroprevalence of ovine brucellosis by modified Rose Bengal test and ELISA in Southern Punjab, Pakistan. Pakistan Veterinary Journal, (2013); 33: 455-457.

Ali S, Ali Q, Abatih EN, Nemat U, Ali M, Khan I, Shamim A. Sero-prevalence of Brucella Abortus among dairy cattle and buffaloes in Pothohar Plateau, Pakistan. Pakistan Journal of Zoology, 2013; 45: 1041-1046.

Rahman MM, Haque M, Rahman MA. Sero-prevalence of caprine and human Brucellosis in some selected areas of Bangladesh. Bangladesh Veterinary Journal, (1988); 22: 85-92.

Al-Majali AM. Seroepidemiology of caprine brucellosis in Jordan. Journal of Small Ruminant Research, (2005); 58: 13-18.

Munir R, Umer F, Zahida F, Afzal M, Zubair A, Jahangir M. (2011). Sero-prevalence of brucellosis in bovines at farms under different management conditions. British Journal of Dairy Sciences, (2011); 2: 35-39.

Sarumathi C, Reddy TV, Sreedevi B. Serological survey of bovine Brucellosis in Andhra Pradesh. Indian Journal of Dairy Science, (2003); 56: 408-410.

Abubakar M, Mehwish M, Muhammad JA. Bovine brucellosis: old and new concepts with Pakistan perspective. Pakistan Veterinary Journal, (2011); 34: 2074-2081.

Belal SMS, Ansari ARM. Seroprevalance of Brucella Abortus antibodies in the selected cattle population in the upazilas of sirajgonj district. Bangalian Journal of Veterinary Medicine, (2013); 11: 127-130.

Rahman MS, Faruk MO, Kim JY, Kang SI, Jung SC. (2011). Prevalence of brucellosis in ruminants in Bangladesh. Veterinarian Medicina, (2011); 56: 379–385.

Salari MH, Khalil MB, Hassan PGR. Selected Epidemiological features of human Brucellosis in Yazd, Islamic Republic of Iran 1993-1998. East Mediator Health Journal, (2003); 9: 1054-1060.


Refbacks

  • There are currently no refbacks.