Biological movement of Xanthomonas oryzae pv. oryzae in Pakistan; A pioneer project of CEMB, Punjab, Pakistan

Ishrat Aziz, Saadat Ali, Zia Rehman, Sheikh Riazuddin, Ahmad Ali Shahid


Background: In Asia, germ plasm exchange in different ecosystems, has been observed. This exchange causes movement of different pathogens. In present study, we worked on movement of Xanthomonas oryzae pv. oryzae (Xoo) causing bacterial blight of rice. In 1976, this disease was first recorded from Punjab (Pakistan) but the cultivars of that time showed resistance against bacterial blight. Then in 1984, the disease was seen on IRRI9. Tremendous increase of this pathogen has become prevalent due to increase in demand of Basmati 385 (cultivar), and now it is one of the most important rice diseases in Pakistan. Hypothesis of present study is “there is genetic diversity of Xoo in different rice growing areas of Punjab, Pakistan.”

Methods: By fingerprinting; the movement pattern of Xanthomonas oryzae pv. oryzae were searched out in present study. The IS1112; a repetitive element of Xanthomonas oryzae pv. oryzae was used to fingerprint twenty-one bacterial strains.

Results: We compared local strains with that of provided by IRRI Philippine (International Rice Research Institute, Philippine). The obtained clusters were correlated with regional differentiation.

Conclusion: Present reported work is the pioneer study (1998-2000) which clued the occurrence of regional movement of pathogen via germ plasm exchange. This study may also provide help to forensics to watch the bioterrorism.

Keywords: Bacterial blight; Xanthomonas oryzae; Germ plasm; Genetic diversity; SAARC 

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Mew T. Current status and future prospects of research on bacterial blight of rice. Annual review of phytopathology, (1987); 25(1): 359-382.

Adhikari TB, Cruz C, Zhang Q, Nelson R, Skinner D, et al. Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Applied and Environmental Microbiology, (1995); 61(3): 966-971.

Mahajan G, Matloob A, Singh R, Singh VP, Chauhan BS. Basmati rice in the Indian subcontinent: Strategies to boost production and quality traits. Advances in Agronomy, (2018); 151159-213.

Muneer N, Rafi A, Akhtar MA. Isolation and characterization of Xanthomonas oryzae pv. oryzae isolates from North West Frontier Province (NWFP), Pakistan. Sarhad Journal of Agriculture, (2007); 23(3): 743.

Arshad HMI, Saleem K, Khan JA, Rashid M, Atiq M, et al. Pathogenic diversity of Xanthomonas oryzae pv. oryzae isolates collected from Punjab Province of Pakistan. European journal of plant pathology, (2017); 147(3): 639-651.

Alam MS, Islam MR, Hossain I, Bhuiyan M, Khan M. Pathotypic variation of Xanthomonas oryzae pv. oryzae in Bangladesh. Archives of Phytopathology and Plant Protection, (2016); 49(1-4): 31-42.

George M, Bustamam M, Cruz W, Leach J, Nelson R. Movement of Xanthomonas oryzae pv. oryzae in Southeast Asia detected using PCR-based DNA fingerprinting. Phytopathology, (1997); 87(3): 302-309.


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