Phytochemical Analyses for Antibacterial Activity and Therapeutic Compounds of Convolvulus arvensis L., Collected from the Salt Range of Pakistan

Muhammad Umair Khan, Noor-Ul-Huda Ghori, Muhammad Qasim Hayat


Background: Pharmaceutical world is currently facing a major issue of bacterial resistance against antibiotics. For the past few years, plants being an excellent source of phytochemicals have replaced many traditional antibacterial agents. Keeping this in view, the current study was conducted to detect the antibacterial activity and the presence of various phytochemicals in Convolvulus arvensis leaves and stem extracts.

Methods: Eight different organic and aqueous extracts of Convolvulus arvensis L. (C. arvensis) leaves and stem were prepared and their antimicrobial activity against 13 clinically important bacterial species was investigated using the disc diffusion assay. Phytochemical screening of the aqueous extracts of C. arvensis was performed using qualitative tests. 

Results: All the thirteen bacterial species tested in this study were found sensitive to the stem and leaf extracts of C. arvensis. Highest antimicrobial activity was observed against   Escherichia coli while among all the extracts butanol extract was the most effective antimicrobial agent. Phytochemical analyses using organic and aqueous extracts confirmed the presence of various secondary plant metabolites of therapeutic value i.e. saponins, flavonoids, steroids, tannins, phlobatannins, cardiac glycosides and coumarins.

Conclusion: Our results indicate that C. arvensis plant contains some phytochemicals with antimicrobial affects. These chemicals possess great potential to be used as advanced therapeutic compounds against broad range of pathogenic bacteria.

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Iqbal H, Sher Z, Khan ZU. Medicinal plants from salt rage Pind Dadan Khan, district Jhelum, Punjab. Pakistan. Journal of Medicinal Plants Research, (2011); 5(11): 2157-2168.

Murad W, Ahmad A, Gilani SA, Khan MA. Indigenous knowledge and folk use of medicinal plants by the tribal communities of Hazar Nao Forest, Malakand District, North Pakistan. Journal of Medicinal Plants Research, (2011); 5(7): 1072-1086.

Singh PK, Kumar V, Tiwari RK, Sharma A, Rao CV, et al. Medico-ethnobotany of ‘Chatara’ Block of district Sonebhadra, Uttar Pradesh, India. Advances in Biological Research, (2010); 4(1): 65-80.

Asad Ullah, Rashid A. Weeds and livelihood in Mankial valley, Hindukush range, Pakistan. Pakistan Journal of Weed Research, (2007); 13(1-2): 27-32.

Akhtar N, Begum S. Ethnopharmacological important plants of Jalala, district Mardan, Pakistan. Pakistan Journal of Science, (2009); 15(2): 95-100.

Hussain A, Khan MN, Iqbal Z, Sajid MS. An account of the botanical anthelmintics used in traditional veterinary practices in Sahiwal district of Punjab. Pakistan Journal of Ethanopharmacology, (2008); 119: 185-190.

Oudhia P, Tripathi RS. Medicinal weeds of kharif crops in the plains of Chhattisgarh. Bhartiya Krishi Anusandhan Patrika, (1998); 13(1-2): 33-38.

Calvino N. Anti angiogenesis properties of a common weed Convolvulus arvensis. Journal of Chiropractic Medicine, (2002); 1(3): 116.

Meng XL. Riordan NH, Casciari JJ, Zhu Y, Zhong J, et al. Effects of a high molecular mass Convolvulus arvensis extract on tumor growth and angiogenesis. Puerto Rico Health Sciences Journal, (2002); 21: 323-328.

Riordan NH, Menh X, Taylor P, Riordan HD. Anti-angiogenic, anti-tumor and immunostimulatory effects of a nontoxic plant extracts (PMG). Comprehensive Cancer Care, (2000).

Malika N, Mohamed F, Chakib A. Antimicrobial Activities of Natural Honey from Aromatic and Medicinal Plants on Antibioresistant Strains of Bacteria. International Journal of Agriculture and Biology, (2004); 6: 289–293.

Harborne JB. Photochemical Methods: A Guide to Modern Techniques of Plant Analysis. 1973; 279. Chapman A. & Hall.

Gibbs RD. Chemotaxonomy of flowering plants. 1974; Vols I-IV: 1-680. McGill-Queen's univ. Press: Montreal & London.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, (2005); 4(7):685-688.

Sofowora A. Medicinal Plants and Traditional Medicines in Africa. (1993); 97-145. Chichester John Wiley & Sons New York.

Kumar A, Ilavarasn R, Jayachandran T, Decaraman M, Aravindhan P, et al. Phytochemicals investigation on a tropical plant, Syzygium cumini from Kattuppalayam, Erode district, Tamil Nadu, South India. Pakistan Journal of Nutrition, (2009); 8(1): 83-85.

Mojab F, Kamalinejad M, Ghaderi N, Vahidipour HR. Phytochemical screening of some species of Iranian plants. Iranian Journal of Pharmaceutical Research, (2010); 2(2): 77-82.

Paris R, Moyse H. Precis de matiere medicinale. (1969); Paris: Masson.

Rizk AM. Constituents of plants growing in Qatar. Fitoterapia, (1982); 52: 35-42.

Raza M, Fozia, Rehman A, Wahab A, Iqbal H, et al. Comparative antibacterial study of Convolvulus arvensis collected from different areas of Khyber Pakhtunkhwa, Pakistan. International research journal of Pharmacy, (2012); 3(10): 220-222.

Zobayed SMA, Afreen F, Kozai T. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s wort. Plant Physiology and Biochemistry, (2005); 43(10): 977-984.

Kirakosyan A, Seymour E, Kaufman PB, Warber S, Bolling S, Chang SC. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (hawthorn) subjected to drought and cold stress. Journal of Agricultural and Food Chemistry, (2003); 51(14): 3973-3976.

Pichersky E, David RG. Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends in Plant Science, (2000); 5(10): 439-445.

Thakral J, Borar S, Kalia JAR. Antioxidant potential fractionation from methanol extract of aerial parts of Convolvulus arvensis Linn. (Convolvulaceae). International Journal of Pharmaceutical Science and Drug Research, (2010); 2(3): 219-23.

Kaur M, Kalia A. Convolvulus arvensis: A useful weed. International Journal of Pharmacy and Pharmaceutical Sciences, (2012); 4(1): 38-40.

Elzaawely AA, Tawata S. Antioxident Activity of Phenolic Rich Fraction obtained from Convolvulus arvensis L. leaves grown in Egypt. Asian Journal of Crop Science, (2012); 4(1): 32-40.

Mahmoudi M, Zamani TRS, Zamani TR, S, Emami SA. A study to investigate the biological activity of proteoglycan mixture extract from Convolvulus arvensis. Journal of Complementary and Integrative Medicine, (2014); 11(4): 265-272.

Hashem FM, El-Kiey MA. Nigella sativa seeds of Egypt. Journal of Pharmaceutical Sciences, (1962); 3(1): 121-133.


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