Response of antioxidants and Reactive oxygen species at various exogenous PEG and proline levels in rice callus

Muhammad Ilyas Khokhar, Imran Habib, Muhammad Jamshaid Anwar, Sajid Rehman


Background: Rice is a water loving crop and drought stress at any physiological stage can hamper the growth as well as its yield. Hence this research was conducted to assess the drought tolerance capability of elite basmati rice cultivars of Pakistan.

Methods: Polyethylene glycol (PEG) mediated in vitro experiment was conducted to estimate various water stress related parameters like callogenesis, crude protein, total antioxidant 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and proline accumulation in callus. Dehusked seeds of five rice genotypes i.e. Basmati-2000, Super Basmati, Basmati-515, Basmati-385 and PS-2 were cultured for callogenesis on Murashige and Skoog (MS) medium having 2,4-Dichlorophenoxyacetic acid (2,4-D) @ 4mg/L, proline @ 1mg/L and two levels of PEG (0.125g/L and (0.25g/L).

Results: In case of relative performance under all treatments, maximum callus fresh weight (0.308g) was observed in PS-2 followed by Basmati-515 (0.281g) and minimum (0.198g) in Basmati-385 after 30 days of culturing. Maximum proline (72.29 μmol/g FW), crude protein (5.89%) and total antioxidant DPPH (70.77%) were noted in Basmati-2000 while maximum H2O2 (75.55 μmol/g FW) was observed in PS-2 and minimum (37.22 μmol/g FW) in Basmati-2000. It was observed that under both PEG treatments, the rice cultivar Basmati-2000 was found to be highly drought tolerant. Maximum callus fresh weight was observed in the treatment without the addition of PEG and proline. Maximum production of Proline, protein and DPPH were observed at a low level of PEG (0.125g/L) in presence of proline @ 1mg/L.

Conclusion: Results revealed that application of proline in rice may be effective in mitigating the effect of drought stress in the presence of PEG under in-vitro conditions.

Keywords: Basmati rice; Callogenesis; DPPH; Hydrogen peroxide; Protein 

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