Synteny of Cotton SSR Markers Genomes Paves the Way for Resistance Against Black Root Rot Disease in Cotton

Trinh Ngoc Ai, Anh Phu Nam Bui


Background: Black root rot disease is documented for substantial reducing cotton yield and fiber quality. The isolation of candidate resistant genes in tetraploid genome AADD cotton species (2n=4x=52) remains challenging in the absence of research of black root rot resistance on progenitor DD genome diploid cotton. In this study, by exploiting Phytozome database, a comparative map of the black root rot-resistance quantitative trait loci in DD genome was constructed.

Methods: Simple sequence repeats markers associated with these three quantitative trait loci in the AA genome were used as “anchored-probes” frameworks for establishing relationships between the two cotton genomes AA and DD.

Results: Our findings showed that there was conserved orders among mapped simple sequence repeats markers on AA genome and the physical map of these simple sequence repeats markers on DD genome.

Conclusion: It was suggested that the syntenic loci on chromosome 2, 7 and 11 on DD genome could harbor the resistance gene against the black root rot disease. This study could serve as a fundamental step in isolating and introducing the resistance gene against black root rot into elite cotton cultivars.

Keywords: Comparative mapping; Resistance gene; Phytozome; Simple sequence repeats; Quantitative trait loci     

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