Construction and characterization of the ex-situ modified macroporous bacterial cellulose scaffold as a potential epidermal graft

Aylin Basaran Eroglu, Gökhan Coral, Gülsen Bayrak, Sakir Necat Yilmaz

Abstract


Background: Skin is a 3-dimensional (3-D) tissue that mainly consists 2 layers, comprising the epidermis and dermis. Skin tissue engineering scaffolds are used commonly as 3-D analogs of the extracellular matrix (ECM) of the skin. Bacterial cellulose (BC) has great importance in skin tissue engineering because of its resemblance to ECM and its biocompatibility. The lack of 3-D microporosity and limited biodegradation capacity has restricted its application as a scaffold for skin tissue engineering. Controlled 3-D microporosity of BC via surface modification techniques are required for potential tissue engineering applications.

Methods: Freeze-drying is an ex-situ surface modification technique for making macroporous BC scaffolds (MBCSs). This study proposed a new approach to the freeze-drying method for the arrangement of the pore size of MBCSs specifically for the human keratinocyte cell line (KER-CT). Different concentrations of MBCS (0.25%, 0.50%, and 0.75%) were prepared and the KER-CT cell viability was detected via 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay.

Result: The results of this study indicated that the KER-CT cells were able to proliferate all of the concentrations of MBCS, and the best cell viability value was observed with 0.75% MBCS. The results were supported by FESEM and light microscopic observations.

Conclusion: These findings suggested that 0.75% MBCS might be of use in epidermal tissue engineering applications.


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References


Horch RE, Kopp J, Kneser U, Beier J, Bach A D. Tissue engineering of cultured skin substitutes. Fundamentals of Tissue Engineering and Regenerative Medicine, (2009); 9: 329–343.

Bottcher-Haberzeth S, Biedermann T, Reichmann E. Tissue Engineering of Skin. Principles of Regenerative Medicine, (2010); 36: 450–460.

Metcalfe AD, Ferguson MWJ. Tissue engineering of replacement skin: The crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration. Journal of the Royal Society Interface, (2007); 4: 413–417.

Bi H, Jin Y. Current progress of skin tissue engineering: Seed cells, bioscaffolds, and construction strategies. Burns & trauma, (2013); 1: 63-72

Priya S G, Jungvid H, Kumar A. Skin tissue engineering for tissue repair and regeneration. Tissue Engineering - Part B: Reviews, (2008); 14: 105–118.

de Oliveira Barud H G, da Silva RR, da Silva Barud H, Tercjak A, Gutierrez J, Lustri W R, de Oliveria Junior O B, Ribeiro, S. J. A multipurpose natural and renewable polymer in medical applications: Bacterial cellulose. Carbohydrate Polymers, (2016); 153: 406-420.

Yu JR, Navarro J, Coburn J C, Mahadik B, Molnar J, et al. Current and future perspectives on skin tissue engineering: key features of biomedical research, translational assessment, and clinical application. Advanced healthcare materials, (2019); 8: 1801471.

Groeber F, Holeiter M, Hampel M, Hinderer S, Schenke-Layland, K. Skin tissue engineering - In vivo and in vitro applications. Advanced Drug Delivery Reviews, (2011); 63: 352–366.

Czaja WK, Young DJ, Kawecki M, Brown R M. (2007). The Future Prospects of Microbial Cellulose in Biomedical Applications, (2007); 8: 1–12.

Fu L, Zhang J, Yang G. Present status and applications of bacterial cellulose-based materials for skin tissue repair. Carbohydrate Polymers, (2013); 92: 1432–1442.

Jozala AF, de Lencastre-Novaes LC, Lopes AM, de Carvalho Santos-Ebinuma V, Mazzola P G, Pessoa-Jr A, Grotto D, Gerenutti M, Chaud MV. Bacterial nanocellulose production and application: a 10-year overview. Applied Microbiology and Biotechnology, (2016); 100: 2063–2072.

Petersen N, Gatenholm P. Bacterial cellulose-based materials and medical devices: Current state and perspectives. Applied Microbiology and Biotechnology, (2011); 91: 1277–1286.

Luo H, Xiong G, Hu D, Ren K, Yao F, Zhu Y, Gao C, Wan Y. Characterization of TEMPO-oxidized bacterial cellulose scaffolds for tissue engineering applications. Materials Chemistry and Physics, (2013); 143: 373-379.

Gao C, Wan Y, Yang C, Dai K, Tang T, Luo H, Wang J. Preparation and characterization of bacterial cellulose sponge with hierarchical pore structure as tissue engineering scaffold. Journal of Porous Materials, (2011); 18: 139–145.

Xiong G, Luo H, Zhu Y, Raman S, Wan Y. Creation of macropores in three-dimensional bacterial cellulose scaffold for potential cancer cell culture. Carbohydrate Polymers, (2014); 114: 553–557.

Stumpf TR, Yang X, Zhang J, Cao X. In situ and ex situ modifications of bacterial cellulose for applications in tissue engineering. Materials Science and Engineering C, (2018); 82: 372–383.

Cai Z, Kim J. Bacterial cellulose/poly(ethylene glycol) composite: Characterization and first evaluation of biocompatibility. Cellulose, (2010); 17: 83–91.

Tao K, Bai XZ, Zhang ZF, Shi J H, Hu XL, Tang C W, Hu DH, Han, JT. Construction of the tissue engineering seed cell (HaCaT-EGF) and analysis of its biological characteristics. Asian Pacific Journal of Tropical Medicine, (2010); 6: 893–896.

Hestrin S, SchrammM. Synthesis of cellulose by Acetobacter xylinum and Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochemical Journal, (1954); 58: 345.

Basaran Eroglu A, Coral G. Preparation and characterization of a 3-dimensional macroporous bacterial cellulose scaffold for in vitro tissue engineering applications. Digest Journal of Nanomaterials and Biostructures, (2021); 16: 1011 - 1017.

Sanchavanakit N, Sangrungraungroj W, Kaomongkolgit R, Banaprasert T, Pavasant P, Phisalaphong M. Growth of human keratinocytes and fibroblasts on bacterial cellulose film. Biotechnology progress, (2006); 22: 1194-1199.

Khan S, Ul-Islam M, Ikram M, Islam S U, Ullah M W, Israr M, Yang J H, Yoon S, Park J K. Preparation and structural characterization of surface modified microporous bacterial cellulose scaffolds: A potential material for skin regeneration applications in vitro and in vivo. International journal of biological macromolecules, (2018); 117: 1200-1210.


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