The effect of Syzygium aromaticum (clove) on inflammatory markers (total leukocyte count, differential leukocyte count and tumor necrosis factor-alpha)

Saima Tabassum, Ambreen Anjum, Sobia Manzoor, Wardah Anwar, Muhammad Hashim Ghouri


Background: Inflammation is involved in pathogenesis of many diseases. Antiinflammatory chemicals can be used to treat such illness,especially if they are derived from plant sources as they will have fewer side effects.

Objective:to find out  the outcome of ethanolic extract of syzygium aromaticum flower buds on markers of inflammation in albino rats.

Material and method: Antiinflammatory activity was investigated in albino rats using a experimental model of  inflammation, the “formalin test” (injecting 5 % formalin into subplantar surface of right hind paw). Ninety rats were uniformly divided into three groups designates as control A,experimental B and refrence C. Intraperitoneal  injection of normal saline, syzygium aromaticum flower bud extract and  indomethacin was used. In these rats, foot edema was measured by Vernier caliper. Blood sampling was done through cardiac punture under anaesthesia to determine total and differential leukocyte counts and serum tumor necrosis factor alpha (TNF-α) levels.

 Results: The clove extract  produced significant (p=0.00) decrease  in inflammatory response initiated by 5% formalin. The extract significantly decreased (p=0.009) serum TNF-α. However, its effect on the total and differential leukocyte counts was non-significant (p˃0.05).

Conclusion: The ethanolic extract of syzygium aromaticum  possesses potent  anti-inflammatory effects.

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Abdulkhaleq LA, Assi MA, Abdullah R, Saad MZ, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Veterinary World, (2018); 11(5):627–35.

Netea MG, Balkwill F, Chonchol M, Cominelli F, Donath MY, Giamarellos-Bourboulis EJ, et al. A guiding map for inflammation. Nat. Immunol. 2017; 18(8): 826–31.

Yang P, Li Y, Xie Y, Liu Y. Different faces for different places: heterogeneity of neutrophil phenotype and function. Journal of Immunology Research, (2019); 8016254.

van Grinsven E, Textor J, Hustin LS, Wolf K, Koenderman L, et al. Immature neutrophils released in acute inflammation exhibit efficient migration despite incomplete segmentation of the nucleus. The Journal of Immunology, (2019); 202(1): 207-217.

Kunchok A, Aksamit AJ, Davis JM, Kantarci OH, Keegan BM, et al. Association between tumor necrosis factor inhibitor exposure and inflammatory central nervous system events. JAMA Neurology, (2020); 77(8): 937-46.

Gudes S, Barkai O, Caspi Y, Katz B, Lev S, et al. The role of slow and persistent TTX-resistant sodium currents in acute tumor necrosis factor-α-mediated increase in nociceptors excitability. Journal of Neurophysiology, (2015); 113(2): 601-619.

Bignami F, Rama P, Ferrari G. Substance P and its inhibition in ocular inflammation. Current Drug Targets, (2016); 17(11):1265-74.

Cruceriu D, Baldasici O, Balacescu O, Berindan-Neagoe I. The dual role of tumor necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches. Cell Oncology, (2020); 43(1):1-18.

Taher YA, Samud AM, El-Taher FE, Ben-Hussin G, Elmezogi JS, et al. Experimental evaluation of anti-inflammatory, antinociceptive and antipyretic activities of clove oil inmice. Libyan Journal of Medicine, (2015); 10(1): 28685.

Chandran R, George BP, Abrahamse H. Anti-proliferative, analgesic and anti-inflammatory properties of Syzygium mundagam bark methanol extract. Molecules, (2020); 25(12): 2900.

Antonisamy P, Dhanasekaran M, Kim H-R, Jo S-G, Agastian P, et al. Anti-inflammatory and analgesic activity of ononitol monohydrate isolated from Cassia tora L. in animal models. Saudi journal of biological sciences, (2017); 24(8): 1933-1938.

Guyton AC, Hall JE. Text Book of Medical Physiology. 14th ed.2019;460-1. Philadelphia: Elsevier.

Batiha GES, Alkazmi LM, Wasef LG, Beshbishy AM, Nadwa EH, et al. Syzygium aromaticum L. (Myrtaceae): Traditional uses, bioactive chemical constituents, pharmacological and toxicological activities. Biomolecules, (2020); 10(2): 202.

Haro-Gonzalez JN, Castillo-Herrera GA, Martinez-Velazquez M, Espinosa-Andrew H. Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food application, and essential bioactivity for human health. Molecules, (2021); 26(21): 6387.

Barboza JN, da Silva Maia Bezerra Filho C, Silva RO, Medeiros JVR, de Sousa DP. An overview on the anti-inflammatory potential and antioxidant profile of eugenol. Oxidative medicine and cellular longevity, (2018); 3957262

Agbaje EO, Adeneye AA, Daramola AO. Biochemical and toxicological studies of aqueous extract of Syzigium aromaticum (L) Merr. & Perry (Myrtaceae) in rodents. African Journal of Traditional, Complementary and Alternative Medicines, (2009); 6: 241-54.

Zari AT, Zari TA, Hakeem KR. Anticancer Properties of Eugenol: A Review. Molecules, (2021); 26(23): 7407.

Aburel OM, Pavel IZ, Dănilă MD, Lelcu T, Roi A, et al. Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown. Oxidative Medicine and Cellular Longevity, (2021): 1-15.

Barnabei L, Laplantine E, Mbongo W, Rieux-Laucat F, Weil R. NF-κB: At the borders of autoimmunity and inflammation. Frontiers in Immunology, (2021); 12: 716469.

Rasmi RR, Sakthivel KM, Guruvayoorappan. NF-B inhibitors in treatment and pprevention of lung cancer. Biomedicine & Pharmacotherapy, (2020); 130: 110569.

Cristol H. How does TNF cause inflammation?. [Updated 2020 August, Cited 2022 March] Available from: []


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