Biosurfactant Producing Bacteria Associated with Oil Polluted Soils of some Auto Mechanic Workshops in Akure, Ondo State, (Southwest) Nigeria

Oluwatoyin Folake Olukunle, Bukola Rukayat Olowoyeye, Temitope Ojuolape Fadipe


Background: Auto mechanic shops account for a sizable fraction of hydrocarbon leaks that go unnoticed most of the time. This study looked into the biosurfactant-producing activity of hydrocarbon using bacteria isolated from five auto mechanic shops in Akure.

Methods: The bacteria were isolated using standard microbiological procedures. The isolates' oil degrading activity was evaluated on Bushnell Haas medium enhanced with 2% petroleum hydrocarbons (petrol, diesel, crude oil and kerosene). Biosurfactant activity was determined using the drop collapse test, oil spread assay, foaming property, and emulsification activity (E24). Biosurfactant-producing isolates were identified using molecular and morphological techniques, as well as biochemical and biochemical assays. Using universal primers, the isolates' hypervariable 16SrRNA region was amplified and sequenced.

Result: Bacillus paramycoides strain OFOa and Clostridium punense strain OFOb were discovered as the two (2) bacterial isolates with high biodegradation and biosurfactant generating activities in this study. Their sequences had since been submitted to GenBank, with accession numbers MN700654 and MN700653, respectively.

Conclusion: As bio-emulsifiers and biosurfactant makers, the strains of these two (2) bacteria identified in this investigation could be used in bioremediation of hydrocarbon-polluted locations.

Full Text:



Dixit H, Lowry M, Mohsin U, Moond M, Kumar S, Chokriwal A, Gupta S. Screening and identification of diesel oil degrading bacterial isolates from petroleum contaminated soil of Barmer. Journal of pharmaceutical, chemical and biological sciences, (2018); 6(1):34-40.

Boz DT, Yalcin HT, Corbaci C, Ucar FB. Screening and molecular characterization of polycyclic aromatic hydrocarbons degrading yeasts. Turkish Journal of Biochemistry, (2015); 40(2):105-110.

Unimke AA, Mmuoegbulam AO, Anika OC. Microbial degradation of petroleum hydrocarbons: realities, challenges and prospects. Journal of Biotechnology, (2018); 22(2):1-10.

Pathak C, Mandalia HC. Petroleum industries: Environmental pollution effects, management and treatment methods. International Journal of Separation for Environmental Sciences, (2012); 1(1):55-59.

Muthukamalam S, Sivagangavathi S, Dhrishya D, Rani SS. Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria. Brazilian Journal of Microbiology, (2017); 48:637–647.

Ramseur JL. Oil Spills: Background and Governance. Congressional Research Service, (2017); 1:29.

Kathi S, Khan AB. Enrichment, isolation and identification of polycyclic aromatic hydrocarbon degrading Rhodococcusruber from sediments. International Journal of Scientific and Research Publication, (2013); 3(2):1-7.

Udgire M, Shah N, Jadhav M. Enrichment, isolation and identification of hydrocarbon degrading bacteria. International Journal of Current Microbiology and Applied Sciences, (2015); 4(6): 708-713.

Jyothi K, Babu SK, Clara NK, Kashyap A. Identification and isolation of hydrocarbon degrading bacteria by molecular characterization. Helix, (2012); 2: 105-111.

Hassan A, Taqi Z, Obuekwe C, Al-Saleh E. Characterization of crude oil-degrading bacteria in a crude oil-contaminated and uncontaminated site in Kuwait. WIT Transactions on Ecology and the Environment, (2011); 167:177-186.

Ebakota OD, Osarueme JO, Gift ON, Odoligie I, Joseph OO. Isolation and characterization of hydrocarbon-degrading bacteria in top and subsoil of selected mechanic workshops in Benin City metropolis, Nigeria. Journal of Applied Science and Environmental Management, (2017); 21(4):641-645.

Varjani JS. Microbial degradation of petroleum hydrocarbons (Review). Bioresource Technology, (2017); 223: 277–286.

Ibrahim HMM. Biodegradation of used engine oil by novel strains of Ochrobactrum anthropiHM-1 and Citrobacter freundii HM-2 isolated from oil-contaminated soil. 3 Biotech, (2016); 6:226.

Karlapudi AP, Venkateswarulu TC, Tammineedi J, Kanumuri L, Ravuru BK, et al. Role of biosurfactants in bioremediation of oil pollution-a

review. Petroleum, (2018); 4(3):241–249.

Ron EZ, Rosenberg E. Biosurfactants and oil bioremediation. Current Opinion in Biotechnology, (2002); 13:249-252.

Petti CA, Polage CR, Schreckenberger P. The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. Journal of Clinical Microbiology, (2005); 43(12): 6123–6125.

Olukunle, OF. Phylogenetic analysis of hydrocarbon degrading bacteria associated with crude oil polluted soil from Mesogar community, Delta State, Nigeria. Nigerian Journal of Biotechnology, (2019); 36(2): 62-76.

Borah D, Yadav RNS. Bioremediation of petroleum-based contaminants with biosurfactant produced by a newly isolated petroleum oil degrading bacterial strain. Egyptian Journal of Petroleum, (2017); 26(1), 181–188.

Morikawa M, Hirata Y, Imanaka TA study on the structure-function relationship of lipopeptide biosurfactants. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, (2000); 1488(3): 211-218.

Saadoun I, Aljneibi D, Al Harthi A, Aljunaibi S, Al Dulijan N, et al. Phenotypic, molecular characterization and evaluation of effectiveness for the bioremediation of oil-degrading bacteria isolated from different habitats in the United Arab Emirates. Indian Journal of Science and Technology, (2018); 11(25):1-14.

Abatenh E, Gizaw B, Tsegaye Z, Wassie M. The Role of Microorganisms in Bioremediation- A Review. Open Journal of Environmental Biology, (2017); 2(1): 038-046.

Kachienga L, Jitendra K, Momba M. Metagenomic profiling for assessing microbial diversity and microbial adaptation to degradation of hydrocarbons in two South African petroleum contaminated water aquifers. Scientific Reports, (2018); 8:7564.

Vignesh R, Arularasan A, Gandhiraj V, Deepika RC. Isolation, identification and characterization of potential oil degrading bacteria from oil contaminated sites. International Research Journal of Engineering Technology, (2016); 3(4):2503-2508

Olukunle OF, Boboye B. The molecular succession of bacterial community of crude oil-polluted soil and water samples from Niger Delta Nigeria. British Journal of Applied Science and Technology, (2013); 3(4):777-788.

Joshi PA, Pandey GB. Screening of petroleum degrading bacteria from cow dung. Research Journal of Agricultural Science, (2011); 2 (1): 69-71.

Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinoti MG, et al. Microbial biosurfactants production, applications and future potential. Applied Microbiology and Biotechnology, (2010); 87(2):427-444.

Patowary K, Patowary R, Kalita MC, Deka S. Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Frontiers in Microbiology, (2017); 8(279):1-14.

Silva RD, Almeida CF, Rufino DG, LunaR DJ, Santos M et al. Applications of biosurfactants in the petroleum industry and the remediation of oil spills. International Journal of Molecular Science, (2014); 15(7):12523-12542.

Rahman KSM, Gakpe E. Production, characterization and applications of biosurfactants-review. Biotechnology, (2008); (2): 360-370. DOI: 10.3923/biotech.2008.360.370.

Barin R, Talebi M, Biria D, Beheshti M. Fast bioremediation of petroleum- contaminated soils by a consortium of biosurfactant/bioemulsifier producing bacteria. ‎International Journal of Environmental Science and Technology, (2014); 11:1701-1710.

Lanjekar VB, Marathe NP, Shouche Y, Ranade DR. Clostridium Punense sp. Nov. an obligate anaerobic bacteria isolated from healthy human faeces. International Journal of Systematic Evolution and Microbiology, (2015); 65 (12): 4749–4756.

Olson ND, Morrow JB. DNA extract characterization process for microbial detection methods development and validation. BMC Research Notes, (2012); 5(668):1-14.

Spini G, Spina F, Poli A, Blieux AL, Regnier T, et al. Molecular and microbiological insights on the enrichment procedures for the isolation of petroleum degrading bacteria and fungi. Frontiers in Microbiology, (2018); 9: 2543.


  • There are currently no refbacks.