Determination of total antioxidant content of methanolic extracts of Cynara scolymus, Echinacea purpurea and Portulaca oleracea

Dhiya Altememy, Mahmoud Bahmani, Fadhil Hussam, Yasir Salam Karim, Mustafa M. Kadhim, Waleed K. Khawaja, Noora M. Hameed, Nathera Hussin Alwan, Mohammad Darvishi


Background: The unique properties of medicinal plants particularly their effects on human health have attracted the attention of many researchers. The antioxidant properties of medicinal plants is a strong reason to use them in the food and pharmaceutical industries. Hence, the aim of this study was to evaluate the antioxidant properties of methanolic extracts of Cynara scolymusEchinacea purpurea   and Portulaca oleracea. Aerial parts of C. scolymusE. purpurea and P. oleracea were dried and ground.

Methods: Then, plant samples were prepared using homogenizing plant powders in methanol solution. Finally, the total   antioxidant capacity of the plants was assessed by ferric iron reducing antioxidant power (FRAP) assay.

Results: The results revealed that the total antioxidant capacity was found as 3.45, 1.16 and 1.68 mmol Fe2+/L for C. scolymusE. purpurea and P. oleracea, respectively. Based on our results, C. scolymusE. purpurea and P. oleracea showed a potent antioxidant activity.

Conclusion: It is recommended that utilization of C. scolymusE. purpurea and P. oleracea in food and pharmaceutical industries could possibly possess beneficial health effects.

Keywords: Antioxidant Activity; Medicinal Plants; Cynara scolymusEchinacea purpureaPortulaca oleracea     

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Hormozi M, Marzijerani AS, Baharvand P. Effects of Hydroxytyrosol on Expression of Apoptotic Genes and Activity of Antioxidant Enzymes in LS180 Cells. Cancer Management and Research, (2020);12:7913.

Nori-Garavand R, Hormozi M, Narimani L, Beigi Boroujeni N, Rajabzadeh A, Zarei L, et al. Effect of selenium on expression of apoptosis-related genes in cryomedia of mice ovary after vitrification. BioMed Research International, (2020);3(2): 1.

Ahmadvand H, Tavafi M, Khosrowbeygi A, Shahsavari G, Hormozi M, Beyranvand K, et al. Amelioration of lipid peroxidation in vivo and in vitro by Satureja khozestanica essential oil in alloxan-induced diabetic rats. Journal of Diabetes & Metabolic Disorders, (2014);13(1):1-6.

Hasanvand A, Pirzadroozbahani N, Ahmadizar F, Kharazmkia A, Mir S, Baharvand PA, et al. Evaluation of the antioxidant effects of zolpidem in the rat model of cisplatin-induced nephrotoxicity. Journal of Renal Injury Prevention, (2018);7(4):235-9.

Mohammadrezaei Khorramabadi R, Anbari K, Salahshoor MR, Alasvand M, Assadollahi V, Gholami M. Quercetin postconditioning attenuates gastrocnemius muscle ischemia/reperfusion injury in rats. Journal of Cellular Physiology, (2020);235(12):9876-83.

Bagheri S, Sarabi MM, Khosravi P, Khorramabadi RM, Veiskarami S, Ahmadvand H, et al. Effects of Pistacia atlantica on oxidative stress markers and antioxidant enzymes expression in diabetic rats. Journal of the American College of Nutrition, (2019);38(3):267-74.

Bagheri S, Khorramabadi RM, Assadollahi V, Khosravi P, Cheraghi Venol A, Veiskerami S, et al. The effects of pomegranate peel extract on the gene expressions of antioxidant enzymes in a rat model of alloxan-induced diabetes. Archives of Physiology and Biochemistry, (2021):1-9.

Bagheri S, Moradi Sarabi M, Gholami M, Assadollahi V, Mohammadrezaei Khorramabadi R, Hadipour Moradi F, et al. D-limonene in diabetic rats. J Renal Inj Prev, (2021);10(3):e24-e.

Sarabi MM, Khorramabadi RM, Zare Z, Eftekhar E. Polyunsaturated fatty acids and DNA methylation in colorectal cancer. World journal of clinical cases, (2019);7(24):4172.

Ahmadvand H, Babaeenezhad E, Nasri M, Jafaripour L, Khorramabadi RM. Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury. Journal of Renal Injury Prevention, (2018);8(2):91-7.

Zendedel A, Gharibi Z, Anbari K, Abbaszadeh A, Khorramabadi RM, Soleymaninejad M, et al. Selenium ameliorate peripheral nerve ischemic-reperfusion injury via decreased TNF-α. Biological trace element research, (2017);176(2):328-37.

Hasanvand A, Kharazmkia A, Mir S, Khorramabadi RM, Darabi S. Ameliorative effect of ferulic acid on gentamicininduced nephrotoxicity in a rat model; role

of antioxidant effects. Journal of Renal Injury Prevention, (2018);7(2):73-7.

Delfani S, Mohammadrezaei-Khorramabadi R, Abbaszadeh S, Naghdi N, Shahsavari S. Phytotherapy for Streptococcus pyogenes. Journal of Pharmaceutical Sciences and Research, (2017);9(5):513.

Delfani S, Mohammadrezaei-Khorramabadi R, Ghamari S, Boroujeni RK, Khodabandeloo N, Khorzoughi MG, et al. Systematic review for phytotherapy in Streptococcus Mutans. Journal of Pharmaceutical Sciences and Research, (2017);9(5):552.

Ghamari S, Mohammadrezaei-Khorramabadi R, Mardani M, Shahsavari S. An overview of the most important medicinal plants with anti-toothache property based on ethnobotanical sources in Iran. Journal of Pharmaceutical Sciences and Research, (2017);9(6):796.

Fazeli-Nasab B, Shahraki-Mojahed L, Dahmardeh N. Evaluation of Antimicrobial Activity of Essential Oil and Ethanolic Extract of 10 Medicinal Plants on Rathayibacter tritici and Xanthomonas translucens. Plant Biotechnol Persa, (2022); 4 (1): 9-17.

Beyranvand F, Gharzi A, Abbaszadeh A, Khorramabadi RM, Gholami M, Gharravi AM. Encapsulation of Satureja khuzistanica extract in alginate hydrogel accelerate wound healing in adult male rats. Inflammation and regeneration, (2019);39(1):1-12.

Pourjabali M, Mohammadrezaei-Khorramabadi R, Abbaszadeh S, Naghdi N, Naji-Haddadi S, Bahmani F. Medicinal plants used for hypertension. Journal of Pharmaceutical Sciences and Research, (2017);9(5):537.

Miraj S, Kiani S. Study of therapeutic effects of Cynara scolymus L.: A review. Der Pharmacia Lettre, (2016);8(9):168-73.

Manayi A, Vazirian M, Saeidnia S. Echinacea purpurea: Pharmacology, phytochemistry and analysis methods. Pharmacognosy reviews, (2015);9(17):63.

Dokhani N, Nazer MR, Shokri S, Darvishi M.Determination and Evaluating the Antioxidant Properties of Ziziphus nummularia (Burm.f.) Wight & Arn., Crataegus pontica K.Koch and Scrophularia striata Boiss. Egyptian Journal of Veterinary Sciences 2022; 53(3): 423-429.

Then M. Examination on antioxidant activity in the greater celandine (Chelidonium majus L.) extracts by FRAP method. Acta Biologica Szegediensis, (2003);47(1-4):115-7.

Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clinical interventions in aging, (2018);13:757.

Adwas AA, Elsayed A, Azab A, Quwaydir F. Oxidative stress and antioxidant mechanisms in human body. J Appl Biotechnol Bioeng, (2019);6(1):43-7.

Salehi B, Azzini E, Zucca P, Maria Varoni E, V Anil Kumar N, Dini L, et al. Plant-derived bioactives and oxidative stress-related disorders: A key trend towards healthy aging and longevity promotion. Applied Sciences, (2020);10(3):947.

Gorinstein S, Cvikrová M, Machackova I, Haruenkit R, Park Y-S, Jung S-T, et al. Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits. Food Chemistry, (2004);84(4):503-10.

Ben Salem M, Affes H, Athmouni K, Ksouda K, Dhouibi R, Sahnoun Z, et al. Chemicals compositions, antioxidant and anti-inflammatory activity of Cynara scolymus leaves extracts, and analysis of major bioactive polyphenols by HPLC. Evidence-based Complementary and Alternative Medicine, (2017);2017.

Vamanu E, Vamanu A, Nita S, Colceriu S. Antioxidant and antimicrobial activities of ethanol extracts of Cynara scolymus (Cynarae folium, Asteraceae family). Tropical Journal of Pharmaceutical Research, (2011);10(6):777-83.

Sharif KOM, Tufekci EF, Ustaoglu B, Altunoglu YC, Zengin G, Llorent-Martínez E, et al. Anticancer and biological properties of leaf and flower extracts of Echinacea purpurea (L.) Moench. Food Bioscience, (2021);41:101005.

Wojdyło A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food chemistry, (2007);105(3):940-9.

31.Negahdari, B., Darvishi, M., Saeedi, A.A. Gold nanoparticles and hepatitis B virus. Artificial Cells, Nanomedicine and Biotechnology, (2019); 47(1):469-474.

Darvishi M, Ziari K, Mohebi H, Alizadeh K. Association between iron deficiency anemia and Helicobacter pylori infection among children under six years in Iran. Acta Medica Iranica, (2015); 53(4):220-224.

Jalalmanesh S, Darvishi M, Rahimi M, Akhlaghdoust M. Contamination of senior medical students' cell phones by nosocomial infections: A survey in a university-affiliated hospital in Tehran. Shiraz E Medical Journal, (2017); 18(4): 43920.

Darvishi M, Nazer MR, Alipour MR. Investigating the end of patients suffering from diabetic foot hospitalized in Be'sat hospital of IRIAF from 2009 to 2014. Biomedical Research, (2017); 28(10): 4360-4633.

Pirhadi M, Shariatifar N, Bahmani M, Manouchehri A. Heavy metals in wheat grain and its impact on human health: A mini-review. Journal of Chemical Health Risks, (2021): Apr 6.

Manouchehri A, Ahangar RM, Bigvand P, Nakhaee S, Mehrpour O. Successful treatment of heart failure due to simultaneous poisoning with aluminum phosphide and zinc phosphide: a case report. Iranian Red Crescent Medical Journal, (2019): Mar 1;21(3).

Darvishi M, Forootan M, Nazer MR, Karimi E, Noori M. Nosocomial Infections, Challenges and Threats: A Review Article. Iranian Journal of Medical Microbiology, (2020); 14(2):162-181.

Sedighi M, Namdari M, Mahmoudi P, Khani A, Manouchehri A, Anvari M. An Overview of Angiogenesis and Chemical and Physiological Angiogenic Factors: Short Review. Journal of Chemical Health Risks, (2022): Jan 15.

Forootan M, Tabatabaeefar M, Mosaffa N, Ashkalak HR, Darvishi M. Investigating esophageal stent-placement outcomes in patients with inoperable non-cervical esophageal cancer. Journal of Cancer, (2018); 9(1): 213-218

Darvishi M. Antibiotic resistance pattern of uropathogenic methicillin-resistant staphylococcus aureus isolated from immunosuppressive patients with pyelonephritis. Journal of Pure and Applied Microbiology, (2016); 10(4):2663-2667.

Tavakolpour S, Darvishi M, Mirsafaei HS, Ghasemiadl M. Nucleoside/nucleotide analogues in the treatment of chronic hepatitis B infection during pregnancy: a systematic review. Infectious Diseases, (2018); 50(2):95-106

Darvishi M, Sadeghi SS. Evaluation of association of helicobacter pylori infection and coronary heart disease (chd) among ccu patients. Journal of Pure and Applied Microbiology, (2016); 10(4):2621-2626.

Manouchehri AA, Pirhadi M, Shokri S, Khaniki GJ. The Possible effects of heavy metals in honey as toxic and carsinogenic substances on human health: A systematic review. Uludağ Arıcılık Dergisi, (2021); 21(2):237-46.

Jafari H, Nemati M, Haddad Molayan P, Khaleghi Rostamkolaie L, Hamidi Nejat H. Scolicidal activity of Mesobuthus eupeus venom against the protoscolices of Echinococcus granulosus. Archives of Razi Institute, (2019): 74(2), 183-189.

Salah Najim A, Bahry Al_Sadoon M, Salem Sheet M. Effect of Caraway Seed Extract on the Blood Biochemistry and Antioxidant Capacity among the Hyperoxidative Stress-Induced Rats. Archives of Razi Institute, (2022): 77(2), 553-563.

46.Moharreri M, Vakili R, Oskoueian E, Rajabzadeh G. Evaluation of Microencapsulated Essential Oils in Broilers Challenged with Salmonella Enteritidis: A Focus on the Body’s Antioxidant Status, Gut Microbiology, and Morphology. Archives of Razi Institute, (2022): 77(2), 629-639.

Varadhan S, Venkatachalam R, Perumal S, Ayyamkulamkara S. Evaluation of Oxidative Stress Parameters and Antioxidant Status in Coronary Artery Disease Patients. Archives of Razi Institute, (2022): 77(2), 853-859.

Jafari H, Salabi F. Phylogenetic and Morphological Analyses of Androctonus crassicuda from Khuzestan Province, Iran (Scorpiones: Buthidae). Archives of Razi Institute, (2020): 75(3), 405.

Masoudi R, Dadashpour Davachi N. Effect of Dietary Fish Oil on Semen Quality and Reproductive Performance of Iranian Zandi Rams. Archives of Razi Institute, (2021): 76(3), 621-629.

Kamil Kadhim Lawi Z, Ameen Merza F, Rabeea Banoon S, Al-Saady M, Al-Abboodi A. Mechanisms of Antioxidant Actions and their Role in many Human Diseases: A Review. Journal of Chemical Health Risks, (2021): 11:), 45-57.

Durgawale PP, Patil MN, Joshi SA, Korabu KS, Datkhile KD. Studies on phytoconstituents, in vitro antioxidant, antibacterial, antiparasitic, antimicrobial, and anticancer potential of medicinal plant Lasiosiphon eriocephalus decne (Family: Thymelaeaceae). Journal of Natural Science, Biology and Medicine, (2019): 1;10(1):38.

You SH, Yoon MY, Moon JS. Antioxidant and Anti-inflammatory Activity Study of Fulvic Acid. Journal of Natural Science, Biology and Medicine, (2021): 12(3): 1.

Datkhile KD, Patil SR, Patil MN, Durgawale PP, Jagdale NJ, Deshmukh V. N. Studies on phytoconstituents, In vitro antioxidant, antibacterial, and cytotoxicity potential of Argemone mexicana Linn.(Family: Papaveraceae). Journal of Natural Science, Biology and Medicine, (2020): 11(2), 198.

Ramesh C, Vinithkumar NV, Kirubagaran R. Marine pigmented bacteria: A prospective source of antibacterial compounds. Journal of Natural Science, Biology and Medicine, (2019): 10(2), 104.

Halim S, Jasmi NA, Ridzuan PM, Anna D, Abdullah S, Sina T. Novel potential Centella asiatica extract in ameliorating neurotoxicity induced oxidative stress in chronic morphine dependant rat model. International Journal of Medical Toxicology & Legal Medicine, (2020): 23(3and4), 79-83.

Othman Z, Khalep HRH, Abidin AZ, Hassan H, Fattepur S. The Anti-Angiogenic Properties of Morinda citrifolia. L (Mengkudu) Leaves using chicken chorioallantoic membrane (CAM) assay. Pharmacognosy Journal, (2019) 11(1): 1.

Fattepur S, Kiran Chanabasappa Nilugal T. Theva Darshan, May Florence Dela Cruz Bacayo, Fadli Asmani, Ibrahim Abdullah, Eddy Yusuf, and Prakash Goudanavar. "Toxicological and pharmacological activity of ethanolic extracts of Catharanthus roseus in expermental animals." International Journal of Medical Toxicology & Legal Medicine, (2018): 21, no. 3and4: 141-144.

Solati K, Karimi M, Rafieian-Kopaei M, Abbasi N, Abbaszadeh S, Bahmani M. Phytotherapy for wound healing: The most important herbal plants in wound healing based on iranian ethnobotanical documents. Mini-Reviews in Medicinal Chemistry, (2020); 21(4): 500-519.

Bahmani M., Jalilian A, Salimikia I, Shahsavari S, Abbasi N. Phytochemical screening of two Ilam native plants Ziziphus nummularia (Burm.f.) Wight & Arn. And Ziziphus spina-christi (Mill.) Georgi using HS-SPME and GC-MS spectroscopy. Plant Science Today, (2020); 7(2): 275-280.

Abbasi N, Khalighi Z, Eftekhari Z, Bahmani M. Extraction and phytoanalysis of chemical compounds of Eucalyptus globulus leaf native to Dehloran, Ilam province, Iran by HS-SPME and GC-MS. Advances in Animal and Veterinary Sciences, (2020); 8(6): 647-652.

Aidy A, Karimi E, Ghaneialvar H, Mohammadpour S, Abbasi N. Protective effect of Nectaroscordum tripedale extract and its bioactive component tetramethylpyrazine against acetaminophen-induced hepatotoxicity in rats. Advances in Traditional Medicine, (2020); 20(3): 471-477.

Abbasi N, Khosravi A, Aidy A, Shafiei M. Biphasic response to luteolin in MG-63 osteoblast-like cells under high glucose-induced oxidative stress. Iranian Journal of Medical Sciences, (2016); 41(2): 118-125.

Uddin MK, Juraimi AS, Anwar F, Hossain MA, Alam MA. Effect of salinity on proximate mineral composition of purslane ('Portulca oleracea'L.). Australian journal of crop Science, (2012): 6(12):1732-6.

Alam M, Juraimi AS, Rafii M, Abdul Hamid A, Aslani F, Hasan M, et al. Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected purslane (Portulaca oleracea L.) accessions. BioMed Research International, (2014); Article ID 296063.



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