The effects of vitamin D on Immunoresponsive gene 1 and Krüppel-like Factor 2 protein expression in the lung due to the cadmium poisoning

Nasim Kardan, Zohre Eftekhari, Saeed Ozmaie, Hesam Uddin Hoseinzadeh


Background: Cadmium, a well-known hazardous heavy metal and non-essential component, has several negative health effects. The long-term use of cadmium toxin to develop a pulmonary model, evaluation of Irg1 gene expression and KLF-2 protein and serum IL4 levels following model induction, and evaluation of vitamin D's therapeutic effects in reducing pulmonary and hepatic complications in a mice model have not been studied.

Methods: A total of 40 healthy female C57 black 6 mice weighing 20–25 g and approximately 6–8 weeks’ old were purchased from animal husbandry, Pasteur Institute of Iran. After induction of the model, the mice were assigned to the following groups such as Group 1 (G1): mice were euthanized the day after induction. Group 2(G2): mice were ethically killed 21 days after induction. Group 3(G3) mice were treated with vitamin D and euthanized 5 days after treatment. Group 4(G4): mice were treated with vitamin D and euthanized 21 days after treatment. Vitamin D3 with a concentration of 300,000 units per milliliter, which is equivalent to 7.5 mg per 1 microliter, and 13.5 μl of the main stock is equivalent to 100 ng, which is used for 1 kg of mice. Blood samples were collected to measure serum glutamic-oxaloacetic transaminase, serum glutamic-pyruvic transaminase, and alkaline phosphatase to evaluate liver toxicity.

Results: Based on the results obtained, serum SGPT levels in vitamin D treatment groups did not show a significant decrease compared to cadmium groups (p >0.001). The ALP biomarker in the groups treated with vitamin D was decreased significant in comparison to untreated model groups (p<0.001). While any significant differences were not observed between the Cd model and the Cd model treated with Vitamin D.

Conclusion: This study proved that administration vitamin D to some extent reduces the toxic effects of cadmium on the liver and lung.

Keywords: Ameliorated; Vitamin D; Protein expression; Lung; Cadmium

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