Genetic Diversity and Screening of Rice Germplasm Lines against Grain Discoloration Disease
Abstract
Background: The research was conducted to screen out the diverse rice germplasm lines against grain discoloration disease by using 10 SSR primer pairs and on the basis of various morphological characters to evaluate the diversity of the genotypes at Faculty of Agricultural sciences, University of the Punjab, Lahore.
Methods: Seed morphological traits were measured and recorded under Randomized Complete Block Design (RCBD) experiment. DNA extraction and PCR analysis was done to measure the genotypic characteristics of rice. Genotypic and phenotypic variability was measured by using ANOVA and correlation. Microsatellite markers, also called simple sequence repeats (SSR) were used for screening and to check the genetic diversity of rice.
Results: The PIC values ranged from 0.204 of RM-07 to 0.640 of primer RM-22 along with other genetic characters i.e., number of alleles, percentage of polymorphism chromosomal location etc. The higher PIC (polymorphism information content) value showed the greatest genetic diversity among the genotypes. The polymorphic SSRs produced 3-5 alleles with an average unit of 3.3. The PIC values ranged from 0.204 to 0.640 with an average mean of 0.51. All the genotypes showed significant differences in all the studied traits which gave the significant results and non-significant results at significance level of p<0.05= *; p<0.01=** respectively. A significant association was also observed between seed length with length width ratio, 1000 grain weight (r= 0.1251*; r= 0.1922**) and seed thickness with 1000 grain weight (r=0.1205*). All these traits showed higher variations for different rice lines.
Conclusions: From the study it was concluded that some rice found to be highly resistant (Blue Nile, IR 50, Line 9 (4×15) and Line 12 (4×23) against grain discoloration that could be more useful for the production new rice lines with diverse genetic characteristics.
Keywords: Oryza sativa; DNA; SSR; Germplasm; Traits; Diversity
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Wing RA, Purugganan MD, Zhang Q. The rice genome revolution: from an ancient grain to Green Super Rice. Nature Review Genetics, (2018); 19: 505-517.
Ashfaq MA, Rasheed A, Ali A, Sajjad M, Rasool B, Shaheen S, Shafiq M, Javed MA, Anwar A, Haider MS, Mubashar U. Identifying and characterizing the main causal pathogen responsible for rice grain discoloration in Pakistan. Pakistan Journal of Agricultural Sciences, (2022); 59: 223-230.
Dirchwolf PM, Gutiérrez SA, Carmona MA. Assessment of grain discoloration in the main rice genotypes of Corrientes Province, Argentina. Summa Phytopathologica, (2018); 44:271-273.
Amini MM, Alizadeh MR, Padasht F, Elahinia SA, Khodaparast SA. Rice grain discoloration effect on physical properties and head rice yield in three rice cultivars. Quality Assurance and Safety of and Foods, (2015); 8 (2): 283-288.
Raghu S, Baite MS, Patil NB, Sanghamitra P, Yadav MK, Prabhukarthikeyan SR, Keerthana U, Pandi GG, Aravindan S, Rath PC. Grain discoloration in popular rice varieties (Oryza sativa L) in eastern India, associated mycoflora, quality losses and management using selected biocontrol agents. Journal of Stored Products Research, (2020); 88: 101682.
Debsharma SK, Syed MA, Ali MH, Maniruzzaman S, Roy PR, Brestic M, Gaber A, Hossain A. Harnessing on Genetic Variability and Diversity of Rice (Oryza sativa L.) Genotypes Based on Quantitative and Qualitative Traits for Desirable Crossing Materials. Genes, (2023); 14(1): 1-10.
Alves NB, Balestre M, Pennacchi JP, Fernandes MCN, Castro DG, Botelho FBS. Genetic progress of upland rice (Oryza sativa L.) lines for disease resistance. Plant Breeding, (2020); 139(5): 853-861.
Akter A, Monir MR, Dola NS, Usha MRI, Rashid AQMB. Correlation between Morphological Architecture of Rice Seed and Transmission of Fungal Pathogens. Annual Research & Review in Biology, (2019); 33(4): 1-13.
Ashfaq M, Khan AS, Khan SHU, Ahmad R. Association of various morphological traits with yield and genetic divergence in rice (Oryza Sativa L.). International Journal of Agriculture and Biology, (2012); 14: 55-62.
Gouda BP, Narendrappa T, Chethana BS, Deepak CA. Screening of Rice (Oryza sativa L.) Genotypes against Grain Discoloration Disease. International Journal of Current .Microbiology and Applied Sciences, (2019); 8(7): 1572-1577.
Roy SC, Sharma BD. Assessment of genetic diversity in rice [Oryza sativa L.] germplasm based on agro-morphology traits and zinc-iron content for crop improvement. Physiology and Molecular Biology of Plants, (2014); 20(2): 209-224.
Malavolta VMA, ArrudaSoligo E, Dias DD, Azzinni LE, Bastos CR. Incidência de fungos e Quantificação de danosemSementesgenótipos de arroz. Summa Phytopathologica, (2007); 33: 280-286.
Bodalkar C, Awadhiya GK. Assessment of percent grain discoloration in important rice varieties. International Journal of Current Research Biosciences Plant Biology, (2014); 1:61-64.
Ashfaq M, Mubashar U, Haider MS, Ali M, Ali A, Sajjad M. Grain discoloration: an emerging threat to rice crop in Pakistan. Journal of Animal and Plant Sciences, (2017); 27: 696-707.
Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus, (1990); 12: 1-13.
SAS, Institute Inc. 2008. SAS® 9.2 Enhanced Logging Facilities. Cary, North Carolina, USA
Lee PY, Costumbrado J, Hsu CY, Kim YH. Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, (2012); (62): 1-5. e3923, doi: 10.3791/3923. PMID: 22546956; PMCID: PMC4846332.
Bala A, Pannu PPS. Status of seed discoloration of rice, mycofora associated and its impact on seed health and quality of farmer saved seeds. Seed Research, (2017); 45: 131-135.
Ghazanfar MU, Javed N, Wakil W, Iqbal M. Screening of some fine and coarse rice varieties against bakanae disease. Journal of Agricultural Research, (2013); 51: 41-49.
Dariush S, Darvishnia M, Ebadi AA. Identification of microsatellite markers linked to grain discoloration disease in rice genotypes. Agricultural Biotechnology Journal, (2020); 12(2): 157-182. doi: 10.22103/jab.2020.15160.1190
Chabra R, Vij L. Grain discoloration and its management: an emerging threat to paddy cultivation. Journal of Plant Disease Protection, (2019); 127: 1-8.
Tiwari P, Ba H. Horizontal Gene Transfer and Endophytes: An Implication for the Acquisition of Novel Traits. Plants (2020); 9: 1-13.
Zarbafi SS, Ham JH. An Overview of Rice QTLs Associated with Disease Resistance to Three Major Rice Diseases: Blast, Sheath Blight, and Bacterial Panicle Blight. Agronomy, (2019); 9: 1-35.
Pampana GB, Narendrappa T, Chethana BS, Deepak C. Screening of Rice (Oryza sativa L.) Genotypes against Grain Discoloration Disease. International Journal of Current Microbiology and Applied Sciences, (2019); 8: 1572-1577.
Jan SF, Khan MR, Iqbal A, Khan FU, Ali A. Genetic diversity in exotic oat germplasm & resistance against barley yellow dwarf virus. Saudi Journal of Biological Sciences, (2020); 27(10): 2622-2631.
Awad-Allah MMA, Shafie WWM, Alsubeie MS, Alatawi A, Safhi FA, ALshamrani SM, Albalawi DA, Al-Amrah H, Alshehri D, Alshegaihi RM, et al. Utilization of Genetic Resources, Genetic Diversity and Genetic Variability for Selecting New Restorer Lines of Rice (Oryza sativa L.). Genes, (2022); 13(12): 1-12. 2227.
Zhong H, Liu S, Zhao G, Zhang C, Peng Z, Wang Z, Yang J, Li Y. Genetic Diversity Relationship Between Grain Quality and Appearance in Rice. Frontiers in Plant Sciences, (2021); 12: 1-13. 708996.
Ghazy MI, Salem KFM, Sallam A. Utilization of genetic diversity and marker-trait to improve drought tolerance in rice (Oryza sativa L.). Molecular Biology Reports, (2021); 48(1): 157-170.
DOI: http://dx.doi.org/10.62940/als.v10i2.1553
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