Microbial Status and Resistance Profile of Bacterial Species Isolated from Recreational and Agricultural Sites in Multan

Rabia Sultan, Humaira Yasmeen, Iqra Munir, Fazeel Laraib


Background: Humans are in continuous contact with recreational areas and agricultural fields. However, recent discovery of resistant bacteria in these sites have posed serious threats to the community.

Methods: This study is designed to investigate prevalence of resistant bacteria in agricultural and recreational areas i.e., parks and rivers, to investigate their resistant profiling against antibiotics and metals by culture dependent agar techniques and to investigate effect of local medicinal plants on isolated resistant bacteria.

Results: A total of 106 isolates from 35 water and sediments samples were isolated. A resistant rate of 46.05% was reported against antibiotics; the highest against ampicillin (75%) and the lowest against streptomycin (8.9%). Identified resistant strains were Bacillus megateriumBacillus subtilisKlebsiella pneumoniaeE. coliMicrococcus luteusStaphylococcus spp., Pseudomonas spp., Streptococcus spp. and Staphylococcus aureus. Co-occurrence of metal resistance with antimicrobial resistance was also reported. Ethanolic leaves extracts showed promising results against isolated resistant bacteria. The highest and lowest zone of inhibition was reported in Syzygium cumini (37mm) and Ziziphus mauritiana (10mm).

Conclusion: The finding of this study has demonstrated presence of resistant bacteria in publicly accessed areas that support various needs. The potential of Syzygium cumini, Ficus benghalensis and Psidium guajava against resistant bacteria in combination can be explored as an alternative green method against resistant bacteria.

Keywords: Antibiotic resistance; Irrigational land; Medicinal plants; Metal resistance; Multi drug resistance; River    

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