Terminalia arjuna restores the levels of alkaline phosphatase and aspartate aminotransferase of acetaminophen intoxicated mice

Nida Irshad, Hassan Hameed, Maryam Mumtaz, Zainab Aslam, Andleeb Batool, Imran Sohail

Abstract


Background: Herbal medicines are natural and comparatively safer than conventional treatments and are well-documented for carrying little potential for harm.  Therefore, phytonutrients have occupied a central stage in the therapeutics. Terminalia arjuna, a medicinal plant, has been reported to have homeostatic, laxative, diuretic, antidiabetic, anticancer and cardiotonic actions.

Methods: The current study was designed to investigate the protective role of T. arjuna leaf extract at three dose levels (100, 250, 500 mg/kg body weight) against acetaminophen (250 mg/kg body weight) induced liver damage.

Results: The administration of hepatotoxin (Acetaminophen) resulted in disturbance of hematological and serological profile including alkaline phosphatase (ALP) and aspartate aminotransferase (AST) which was assayed in control and drug treated experimental models. Treatment with T.  arjuna leaf extract for 7 days restored the normal levels of markers and response was dose dependent.

Conclusion: This study adds to the very limited existing literature regarding hepatoprotective effect of T. arjuna against acetaminophen toxicity. It is also important to get a step closer to development of accessible, authoritative, and independent information resources about herbal medicines and wide-ranging health disorders, which are currently lacking in Pakistan.

Keywords: Hepatoprotective; Terminalia arjuna; ALP; AST    


Full Text:

PDF

References


Yousafzai AM, Shakoori A. Hepatic Responses of A Freshwater Fish Against Aquatic Pollution. Pakistan Journal of Zoology, (2011);43(2).

Montanari F, Pinto M, Khunweeraphong N, Wlcek K, Sohail MI, Noeske T, Boyer S, Chiba P, Stieger B, Kuchler K, Ecker GF. Flagging drugs that inhibit the bile salt export pump. Molecular Pharmaceutics, (2016); 13(1): 163-71.

Sohail MI, Mughal MS, Arshad N, Arshad M. Incidence of hepatitis B and C in industrial areas of Sheikhupura and Kasur. Pakistan Journal of Zoology, (2010);42 (6).

Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition journal, (2015);15(1): 1-22.

Prusti A. Antibacterial activity of some Indian medicinal plants. Ethnobotanical leaflets, (2008); 2008(1): 27.

Saleem A, Husheem M, Härkönen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. Journal of Ethnopharmacology, (2002); 81(3): 327-36.

Singh G, Singh AT, Abraham A, Bhat B, Mukherjee A, Verma R, et al. Protective effects of Terminalia arjuna against Doxorubicin-induced cardiotoxicity. Journal of ethnopharmacology, (2008); 117(1): 123-9.

Sivalokanathan S, Ilayaraja M, Balasubramanian M. Efficacy of Terminalia arjuna (Roxb.) on N-nitrosodiethylamine induced hepatocellular carcinoma in rats. (2005).; 43 (03): 264-267.

Rahmatullah M, Noman A, Hossan MS, Rashid M, Rahman T, Chowdhury MH, et al. A survey of medicinal plants in two areas of Dinajpur district, Bangladesh including plants which can be used as functional foods. American Eurasian Journal of Sustainable Agriculture, (2009); 3(4): 862-76.

Rao BK, Sudarshan PR, Rajasekhar M, Nagaraju N, Rao CA. Antidiabetic activity of Terminalia pallida fruit in alloxan induced diabetic rats. Journal of Ethnopharmacology, (2003); 85(1): 169-72.

Jain S, Yadav PP, Gill V, Vasudeva N, Singla N. Terminalia arjuna a sacred medicinal plant: phytochemical and pharmacological profile. Phytochemistry Reviews, (2009); 8: 491-502.

Dwivedi S. Terminalia arjuna Wight & Arn.—a useful drug for cardiovascular disorders. Journal of ethnopharmacology. (2007); 114(2): 114-29.

Haidry MT, Malik A. Hepatoprotective and antioxidative effects of Terminalia arjuna against cadmium provoked toxicity in albino rats (Ratus norvigicus). Biochem Pharmacol, (2014); 3(130): 2167-0501.

Varghese A, Savai J, Pandita N, Gaud R. In vitro modulatory effects of Terminalia arjuna, arjunic acid, arjunetin and arjungenin on CYP3A4, CYP2D6 and CYP2C9 enzyme activity in human liver microsomes. Toxicology Reports, (2015); 2: 806-16.

Soni N, Singh VK. Efficacy and advancement of Terminalia Arjuna in Indian herbal drug research: A review. Trends in Applied Sciences Research, (2019);1 4(4); 233-242.

Chaudari GM, Mahajan RT. In vitro hepatoprotective activity of Terminalia arjuna stem bark and its flavonoids against CCl4 induced hepatotoxicity in goat liver slice culture. Asian Journal of Plant Science & Research. (2016);6: 10-17

Gupta S, Bishnoi JP, Kumar N, Kumar H, Nidheesh T. Terminalia arjuna (Roxb.) Wight & Arn.: Competent source of bioactive components in functional food and drugs. The Pharma Innovation Journal, (2018); 7(3): 223-31.

Khan S, Noor F, Sohail I, Imtiaz S, Anum F, Sarmad S, et al. Hepatoprotective role of fruit extract of Terminalia arjuna in acetaminophen intoxicated mice. Advancements in Life Sciences, (2020); 8(1): 63-7.

Shakya AK, Shukla S. Evaluation of hepatoprotective efficacy of Majoon-e-Dabeed-ul-ward against acetaminophen-induced liver damage: A Unani herbal formulation. Drug development Research, (2011); 72(4): 346-52.

Fountoulakis M, Berndt P, Boelsterli UA, Crameri F, Winter M, Albertini S, et al. Two-dimensional database of mouse liver proteins: Changes in hepatic protein levels following treatment with acetaminophen or its nontoxic regioisomer 3-acetamidophenol. ELECTROPHORESIS: An International Journal, (2000); 21(11): 2148-61.

Girish C, Koner BC, Jayanthi S, Ramachandra Rao K, Rajesh B, Pradhan SC. Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice. Fundamental & clinical pharmacology, (2009); 23(6): 735-45.

Subasini U, Rajamanickam G, Dubey G, Prabu P, Sahayam CS, Shabi MM, et al. Hydroalcoholic extract of Terminalia arjuna: A potential hepatoprotective herb. Journal of Biological Sciences, (2007); 7(2): 255-62.

Yakubu M, Akanji M, Oladiji A. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian Journal of Andrology, (2005); 7(4): 399-404.

Adebayo JO, Adesokan AA, Olatunji LA, Buoro DO, Soladoye AO. Effect of ethanolic extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biokemistri, (2005); 17(1): 45-50.




DOI: http://dx.doi.org/10.62940/als.v10i1.1625

Refbacks

  • There are currently no refbacks.