Comparative Antibacterial Analysis of Four Different Medicinal Plants Against Human Skin Flora

Rabeea Anwar, Humaira Yasmeen, Muhammad Nauman Sharif, Rukkaya Alam, Saigha Marriam, Shakeel Hussain, Iqra Arooj, Zeshan Ali


Background: As we are facing continuously increasing antibiotic resistance, increased infections, diseases and pandemics, the researching community is turning to find the solutions from nature and plants play a vital role in this scenario. Pakistan due to its unique geography and a variety of climatic zones harbors a huge floral diversity.

Methods: In this study extracts of four plants of Pakistan namely Azadirachta indica, Curcuma longa, Moringa oleifera and Eucalyptus camaldulensis prepared by Aqueous. Ethanol and Methanol extract preparation methods were examined for their phytochemical content by employing various reagents and methods like foam test for saponins, xanthoprotein test for proteins, Braemar’s test for tannins etc. Antibacterial activity against stored human skin flora samples was performed using the agar well diffusion assay and statistical analysis was performed using ANOVA one way analysis on Microsoft Excel 2010.

Result: Each plant extract exhibited antibacterial activity and statistical analysis performed for extracts of each plant showed p value for A. indica as 0.36 (P > 0.05), for C. longa as 0.09 (P > 0.05), for M. oleifera as 0.67 (P > 0.05) and for E. camaldulensis as 0.02 (P < 0.05), which indicates significant antibacterial activity of E. camaldulensis depending on the size of zones of inhibition produced by it. 

Conclusion: All the extracts prepared from selected plants showed some degree of antibacterial activity against the human skin flora that can also act as opportunistic pathogen. This supports the use of traditionally used plants and herbs for fighting present day soaring health related issues as antimicrobial resistance. Study also suggests further investigations for estimating exact concentrations of extracts to be used that work efficiently in relevant cases and molecular mechanism of action of these extracts.

Keywords: Skin Flora; Antibiotic Resistant; Plant Extract; Antibacterial Activity, Medicinal plants    

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Khan S, Ur-Rehman T, Mirza B, Ul-Haq I, Zia M. Antioxidant, antimicrobial, cytotoxic and protein kinase inhibition activities of fifteen traditional medicinal plants from Pakistan. Pharmaceutical Chemistry Journal, (2017); 51391-398.

Ahmed N, Karobari MI, Yousaf A, Mohamed RN, Arshad S, et al. The Antimicrobial Efficacy Against Selective Oral Microbes, Antioxidant Activity and Preliminary Phytochemical Screening of Zingiber officinale. Infection and Drug Resistance, (2022); 15: 2773-2785.

Yadav R, Agarwala M. Phytochemical analysis of some medicinal plants. Journal of phytology, (2011); 3(12): 10-14.

Ramzan M, Karobari MI, Heboyan A, Mohamed RN, Mustafa M, et al. Synthesis of silver nanoparticles from extracts of wild ginger (Zingiber zerumbet) with antibacterial activity against selective multidrug resistant oral bacteria. Molecules, (2022); 27(6): 2007.

Javed F, Ali Z, Ali S, Ahmed N, Alam MK, et al. BARLEY BRAN, A NOVEL AGRICULTURAL WASTE FOR THE IMPROVED PRODUCTION OF AN EXTRACELLULAR LACCASE FROM A SOIL-INHABITED Penicillium spp. Journal of microbiology, biotechnology and food sciences, (2022); e3631-e3631.

Tariq F, Ahmed N, Afzal M, Khan MAU, Zeshan B. Synthesis, Characterization and antimicrobial activity of Bacillus subtilis-derived silver nanoparticles against multidrug-resistant bacteria. Jundishapur Journal of Microbiology, (2020); 13(5).

Saleem S, Muhammad G, Hussain MA, Bukhari SNA. A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica. Phytotherapy research, (2018); 32(7): 1241-1272.

Ali Z, Jatoi MA, Al-Wraikat M, Ahmed N, Li J. Time to enhance immunity via functional foods and supplements: Hope for SARS-CoV-2 outbreak. Altern Ther Health Med, (2021); 2730-44.

Shinwari ZK, Qaiser M. Efforts on conservation and sustainable use of medicinal plants of Pakistan. Pak J Bot, (2011); 43(1): 5-10.

Hasan Z, Zeshan B, Hassan A, Daud NHA, Sadaf A, et al. Preparation and characterization of edible whey protein nanofibrils and efficacy studies on the quality and shelf‐life of chilled food products. Journal of Food Safety, (2022); e13034.

Ahmad S, Maqbool A, Srivastava A, Gogol S. Biological detail and therapeutic effect of azadirachta indica (neem tree) products-a review. Evidence Based Med Healthcare, (2019); 6(22): 1607-1612.

Saleem A, Ahotupa M, Pihlaja K. Total phenolics concentration and antioxidant potential of extracts of medicinal plants of Pakistan. Zeitschrift für Naturforschung C, (2001); 56(11-12): 973-978.

Rajkumari S, Sanatombi K. Nutritional value, phytochemical composition, and biological activities of edible Curcuma species: A review. International journal of food properties, (2017); 20(sup3): S2668-S2687.

Dhakad AK, Ikram M, Sharma S, Khan S, Pandey VV, et al. Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytotherapy Research, (2019); 33(11): 2870-2903.

Gandji K, Chadare F, Idohou R, Salako V, Assogbadjo A, et al. Status and utilisation of Moringa oleifera Lam: A review. African Crop Science Journal, (2018); 26(1): 137-156.

Sabo VA, Knezevic P. Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial crops and products, (2019); 132413-429.

Ghareeb MA, Habib MR, Mossalem HS, Abdel-Aziz MS. Phytochemical analysis of Eucalyptus camaldulensis leaves extracts and testing its antimicrobial and schistosomicidal activities. Bulletin of the National Research Centre, (2018); 421-9.

Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. Journal of pharmacy & bioallied sciences, (2020); 12(1): 1.

Roghini R, Vijayalakshmi K. Phytochemical screening, quantitative analysis of flavonoids and minerals in ethanolic extract of Citrus paradisi. International Journal of Pharmaceutical Sciences and Research, (2018); 9(11): 4859-4864.

Sasidharan S, Chen Y, Saravanan D, Sundram K, Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African journal of traditional, complementary and alternative medicines, (2011); 8(1): 1-10.

Akbar A, Ali I, Samiullah NU, Khan SA, Rehman Z, et al. Functional, antioxidant, antimicrobial potential and food safety applications of curcuma longa and cuminum cyminum. Pakistan Journal of Botany, (2019); 51(3): 1129-1135.

Parveen S, Saqib S, Ahmed A, Shahzad A, Ahmed N. Prevalence of MRSA colonization among healthcare-workers and effectiveness of decolonization regimen in ICU of a Tertiary care Hospital, Lahore, Pakistan. Advancements in Life Sciences, (2020); 8(1): 38-41.

Sohail M, Muzzammil M, Ahmad M, Rehman S, Garout M, et al. Molecular Characterization of Community-and Hospital-Acquired Methicillin-Resistant Staphylococcus aureus Isolates during COVID-19 Pandemic. Antibiotics, (2023); 12(1): 157.

Rizvi A, Saeed MU, Nadeem A, Yaqoob A, Rabaan AA, et al. Evaluation of Bi-Lateral Co-Infections and Antibiotic Resistance Rates among COVID-19 Patients in Lahore, Pakistan. Medicina, (2022); 58(7): 904.

Ahmed N, Khalid H, Mushtaq M, Basha S, Rabaan AA, et al. The Molecular Characterization of Virulence Determinants and Antibiotic Resistance Patterns in Human Bacterial Uropathogens. Antibiotics, (2022); 11(4): 516.

Ahmed N, Khan M, Saleem W, Karobari M, Mohamed R, et al. Evaluation of Bi-Lateral Co-Infections and Antibiotic Resistance Rates among COVID-19 Patients. Antibiotics (2022); (11): 276.

Ahmed N, Zeshan B, Naveed M, Afzal M, Mohamed M. Antibiotic resistance profile in relation to virulence genes fimH, hlyA and usp of uropathogenic E. coli isolates in Lahore, Pakistan. Trop Biomed, (2019); 36559-568.

Ahmed N, Tahir K, Aslam S, Cheema SM, Rabaan AA, et al. Heavy Metal (Arsenic) Induced Antibiotic Resistance among Extended-Spectrum β-Lactamase (ESBL) Producing Bacteria of Nosocomial Origin. Pharmaceuticals, (2022); 15(11): 1426.

Ahmed N, Ali Z, Riaz M, Zeshan B, Wattoo JI, et al. Evaluation of antibiotic resistance and virulence genes among clinical isolates of Pseudomonas aeruginosa from cancer patients. Asian Pacific journal of cancer prevention: APJCP, (2020); 21(5): 1333.

Zeshan B, Karobari MI, Afzal N, Siddiq A, Basha S, et al. The usage of antibiotics by COVID-19 patients with comorbidities: the risk of increased antimicrobial resistance. Antibiotics, (2021); 11(1): 35.

Ahmad A, Baig AA, Hussain M, Saeed MU, Bilal M, et al. Narrative on Hydrogen Therapy and its Clinical Applications: Safety and Efficacy. Current Pharmaceutical Design, (2022); 28(31): 2519-2537.

Al-Hatamleh MA, Alshaer W, Ma’mon MH, Lambuk L, Ahmed N, et al. Applications of Alginate-Based Nanomaterials in Enhancing the Therapeutic Effects of Bee Products. Frontiers in Molecular Biosciences, (2022); 9.

Hanif H, Elikaei A, Vazini H, Mohammadi A. Anticancer and Antibacterial Effect of Eucalyptus Camaldulensis, in Vitro. Medical Laboratory Journal, (2021); 15(1): 26-32.

Sakha H, Hora R, Shrestha S, Acharya S, Dhakal D, et al. Antimicrobial activity of ethanolic extract of medicinal plants against human pathogenic bacteria. Tribhuvan University Journal of Microbiology, (2018); 51-6.

Jassal PS, Thambyrajah JC. Antibacterial and phytochemical analysis of condiments. Drug Invention Today, (2018); 10(5): 844-847.

Hosea ZY, Kator L, Rhoda EH. Phytochemical properties and antimicrobial activities of aqueous extract of Curcuma longa (Turmeric) rhizome extract. Asian Journal of Research in Crop Science, (2018); 2(1): 1-8.

Pandey J, Mishra S, Jaiswal K. In vitro evaluation of the anthelmintic activity of rhizome extracts of Curcuma Longa (Linn.). IN VITRO, (2018); 11(12).

Oluwajobi I, Kabiru YA, Jigam AA. Antibacterial and Antifungal activities of aqueous leaves extract of some medicinal plants. GSC Biological and Pharmaceutical Sciences, (2019); 9(1): 062-069.

Al-Mhanna SB, Ghazali WSW, Mohamed M, Rabaan AA, Santali EY, et al. Effectiveness of physical activity on immunity markers and quality of life in cancer patient: a systematic review. PeerJ, (2022); 10e13664.

Negassa Z, Furgasa W, Mekonnen N. Phytochemical screening and in vitro anthelminthic activity of selected ethnoveterinary medicinal plants in Haramaya District, Eastern Hararghe zone, Ethiopia.

Ishag OAO, Erwa IY, Diriye MA, Lawane AAM, Ahmed HM, et al. antimicrobial potential and phytochemical screening of Eucalyptus camaldulensis and Eucalyptus microtheca leaves extracts. South Asian Research Journal of Natural Products, (2018); 1(3): 1-6.

Anigboro AA, Avwioroko OJ, Cholu CO. Phytochemical constituents, antimalarial efficacy, and protective effect of Eucalyptus camaldulensis aqueous leaf extract in plasmodium berghei-infected mice. Preventive nutrition and food science, (2020); 25(1): 58.

Haruna H, Gabi B, Umar H. Phytochemical Analysis and Anti-Malarial Potential of Eucalyptus camaldulensis (Turare) Leaf Fractions.

Kaur S, Gupta S, Gautam PB. Phytochemical analysis of Eucalyptus leaves extract. Journal of Pharmacognosy and Phytochemistry, (2019); 8(1): 2442-2446.

Nigussie D, Davey G, Legesse BA, Fekadu A, Makonnen E. Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients. BMC Complementary Medicine and Therapies, (2021); 211-10.

Ali MJ, Obaid RF, Obaid RF. Antibacterial activity for acne treatment through medicinal plants extracts: novel alternative therapies for acne. Journal of Pure and Applied Microbiology, (2019); 13(2): 1245-1250.

Saxena A, Mukhopadhyay A, Nandi S. Antibacterial activity of selected plants extract against pathogenic bacteria and detection of phytochemicals. Journal of Environmental Biology, (2020); 41(6): 1486-1492.

Yelmate A, Thonte S. Antibacterial activity of some Indian medicinal plants against methicillin resistant Staphylococcus aureus (MRSA). Journal of Pharmacognosy and Phytochemistry, (2019); 8(5): 376-380.

Irshad S, Muazzam A, Shahid Z, Dalrymple MB. Curcuma longa (Turmeric): An auspicious spice for antibacterial, phytochemical and antioxidant activities. Pak J Pharm Sci, (2018); 31(6): 2689-2696.

Hossaini F, Das NC, Hossaini F, Acharjee M, Munshi SK. Antimicrobial traits of different medicinal plants locally available in Bangladesh. Biomedical and Biotechnology Research Journal (BBRJ), (2021); 5(1): 1.

Husein HA, Alhasan DA, Albadry MA. In Vitro Antimicrobial Activity and GC-MS Analysis of Crude Aqueous Methanolic Extract Produced from Leaves of Eucalyptus species. Med J, (2019); 172019.


Seddiek AS, Hamad GM, Zeitoun A, Zeitoun M, Ali S. Antimicrobial and antioxidant activity of some plant extracts against different food spoilage and pathogenic microbes. Eur J Nutr Food Saf, (2020); 121-12.

Al-Hadidy YI, Yaseen SS, Saleh GM. The Inhibitory Effect of some Plant Extracts on some Pathogenic Bacteria. Tikrit Journal of Pure Science, (2019); 24(1): 62-69.

Chiamaka OS, Ndarubu TA, Mahmood MF, Adenike AR, Alfa S, et al. In Vitro Antioxidants, Antimicrobials and Biochemical Response of Methanol Leaf Extract of Eucalyptus camaldulensis following Sub-Acute Administration to Rats. Saudi Journal of Biomedical Research (2019); 4(11): 405-411.


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