The effects of 4β-hydroxy withanolide E extracted from Physalis Peruviana on Complete Blood Count of Dimethylbenz(a)anthracene-induced Breast Cancer in Albino Rats

Fahmida Gul, Samreen Memon, Ikramuddin Ujjan, Pushpa Goswami, Kanwal Abbas Bhatti

Abstract


Background: Several studies reveal changes in blood parameters in patients with cancer. Complete blood count is a good predictor for many cancer diagnoses and prognoses. They are also helpful in assessing drug efficacy and toxicity. Cancers may be treated with bioactive and antioxidant compounds derived from plants. The effects of Physalis peruviana on the complete blood count of dimethylbenz(a)anthracene induced are still not well identified.

Methods: This study was conducted at Liaquat University of Medical and Health Sciences, Jamshoro, for six months on 60 female albino rats divided in 05 groups. The effects of Physalis peruviana fruits and tamoxifen on complete blood count in dimethylbenz(a)anthracene induced breast cancer were assessed. Blood samples were collected at weeks 1, 5, 7, and 10 for hematological changes before, during, and after treatment.

Result: Rats of group E (Tumor-induced albino rats treated with the extracts of Physalis Peruviana and tamoxifen) gave the best results compared to other groups. In this group, increases in weight along with an improvement in hematological parameters were observed when compared with others.

Conclusion: The extract from Physalis Peruviana helps in the reversal of pancytopenia with anemia and thrombocytopenia caused by tumor-inducing drugs.


Full Text:

PDF

References


Hu C, Hart SN, Gnanaolivu R, Huang H, Lee KY, et al. A Population-Based Study of Genes Previously Implicated in Breast Cancer. N Engl J Med, (2021); 384(5): 440-451.

Waks AG, Winer EP. Breast Cancer Treatment: A Review. JAMA, (2019); 321(3): 288-300.

El-Beltagi HS, Mohamed HI, Safwat G, Gamal M, Megahed BMH. Chemical Composition and Biological Activity of Physalis peruviana L. Gesunde Pflanzen, (2019); 71113-122.

Peng F, Setyawati MI, Tee JK, Ding X, Wang J, et al. Nanoparticles promote in vivo breast cancer cell intravasation and extravasation by inducing endothelial leakiness. Nature

Nanotechnology, (2019); 14279-286.

Aitte SA, Zain M. Study Of Changes In Blood Parameters And Calculation Of PCT , MPV And DPW For The Platelets Of Laboratory Females And Males Of Albino Mice During Exposure To Doses Of PyrethriodPesticide ( Alphacypermethrin ). (2019); 14(2): 71--78.

Lodhi M, Shaikh S, Afridi A, Baig MT, Farooq L, et al. Comparison of Anti-Seizure Efficacy of Combined Extract of Swertia chirata and Brassica nigra with Standard Anti-Epileptic Drugs in PTZ Model. Health …, (2019); 8160-167.

Lange ME, Uwiera RRE, Inglis GD. Housing Gnotobiotic Mice in Conventional Animal Facilities. Current Protocols in Mouse Biology, (2019); 9e59.

Suárez AI, Chávez K (2018) Appraisal of Medicinal Plants with Anticancer Properties in South America. Anticancer plants: Properties and Application. Singapore: Springer Singapore. pp. 229-283.

Rani R, Kansal VK. Study on cow ghee versus soybean oil on 7,12-dimethylbenz(a)-anthracene induced mammary carcinogenesis & expression of cyclooxygenase-2 & peroxisome proliferators activated receptor- γ in rats. Indian Journal of Medical Research, (2011); 133497-503.

10. Kumar M, Dandapat S, Prasad Sinha M, Kumar A, Raipat BS. Different blood collection methods from rats: A review. Balneo Research Journal, (2017); 846-50.

Jagtap UB, Panaskar SN, Bapat VA. Evaluation of antioxidant capacity and phenol content in jackfruit (Artocarpus heterophyllus Lam.) fruit pulp. Plant Foods Hum Nutr, (2010); 65(2): 99-104.

Almora Pinedo Y, Arroyo-Acevedo JL, Herrera-Calderon O, Chumpitaz Cerrate VM, Hañari Quispe R, et al. Preventive effect of Oenothera rosea on N-methyl-N-nitrosourea- (NMU) induced gastric cancer in rats. Clinical and Experimental Gastroenterology, (2017); Volume 10327-332.

Arroyo-Acevedo J, Herrera-Calderón O, Chávez-Asmat R, Anampa-Guzmán A, Chumpitaz-Cerrate V, et al. Protective effect of Chuquiraga spinosa extract on N -methyl-nitrosourea (NMU) induced prostate cancer in rats. Prostate International, (2017); 5(2): 47-52.

Joshi S, Kumar S, Ponnusamy MP, Batra SK. Hypoxia-induced oxidative stress promotes MUC4 degradation via autophagy to enhance pancreatic cancer cells survival. Oncogene, (2016); 35(45): 5882-5892.

Mier-Giraldo H, Díaz-Barrera LE, Delgado-Murcia LG, Valero-Valdivieso MF, Cáez-Ramírez G. Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells. Journal of Evidence-Based Complementary & Alternative Medicine, (2017); 22(4): 777-787.

Rana AP, Kaur M, Zonunsanga B, Puri A, Kuka AS. Preoperative Peripheral Blood Count in Breast Carcinoma: Predictor of Prognosis or a Routine Test. Int J Breast Cancer, (2015); 2015964392.

Akuru U. Effect of selected plants on Haematological parameters of DMBA-induced breast cancer of albino rats. International Journal of Biochemistry & Physiology, (2019); 4.

Akinbami A, Popoola A, Adediran A, Dosunmu A, Oshinaike O, et al. Full blood count pattern of pre-chemotherapy breast cancer patients in Lagos, Nigeria. Caspian J Intern Med, (2013); 4(1): 574-579.

Wm A, Dw N. Hematological Markers of In Vivo Toxicity. Journal of Hematology & Thromboembolic Diseases, (2016); 04(02).

Oday Ali L. Study effect of Breast Cancer on Some Hematological and Biochemical Parameters in Babylon Province, Iraq. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS, 9(3): 20-24.

Zingue S, Njuh AN, Tueche AB, Tamsa J, Tchoupang EN, et al. In Vitro Cytotoxicity and In Vivo Antimammary Tumor Effects of the Hydroethanolic Extract of Acacia seyal (Mimosaceae) Stem Bark. BioMed Research International, (2018); 20182024602.

Chen C-H, Wu S-H, Tseng Y-M, Liao J-B, Fu H-T, et al. Suppressive Effects of Puerariae Radix on the Breast Tumor Incidence in Rats Treated with DMBA. Journal of Agricultural Science, (2017); 9(7): 68-68.

Rochet NM, Markovic SN, Porrata LF. The Role of Complete Blood Cell Count in Prognosis—Watch this Space! Oral History Review, (2012); 0876.

Asuquo Etim E, Abiodun Emokpae M, Collins Ohwonigho A, Adebayo Yusuf A. Association of platelet count and platelet indices with stages of women breast cancer in Yola, Nigeria. Hematology & Transfusion International Journal, (2018); 6(1).


Refbacks

  • There are currently no refbacks.