hSSB1 (NABP2) as Prognostic for Worker's Exposure to Oxidative Stress

Rafed Abbas Kadhum, Nasser Nafaa Abrahem Alqurashy, Jinan Tuma Sabah

Abstract


Background: Oxidative stress can be defined as a phenomenon brought on by an imbalance between biological system's capacity for detoxifying such reactive products and the formation and build-up of ROS in tissues and cells. X-rays have an effect on the systems of the human body, hSSB1 (NABP2) plays a crucial role in DNA damage responses by taking part in a repair regarding double-strand DNA breaks and the base excision repair of oxidized guanine residues (8-oxoguanine). Here, we show that hSSB1 stabilizes as an oligomer in response to oxidative stress, which is necessary for the hSSB1 to remove 8oxoguanine.

Methods: In this study, the detection of NABP2 (SSB-I) (Single-stranded DNA binding protein (ATM / MRN path-way)) expression through QRT-PCR is considered a key method. Depending on the Centre for Radiation Protection, employees exposed to weak doses of y -rays approximately (2-10) cGy could experience X-ray Geno toxicity. The exposure group worked in close proximity to the source (X-ray) and control group. Whole blood had been collected from all of the individuals, according to the findings.

Results: NABP2 (hSSBI) expression increased in people who were exposed for longer than lcGy and decreased in the controls. was enhanced as a result of the Geno toxicity caused by low doses of X-rays. X-ray oxidative stress stimulates. Single-stranded DNA (ssDNA)-binding proteins like OBFC2B, which bind to single-stranded DNA (NABP2; SSBI).

Conclusion: In the present study, the conclusion that has been reached is that the exposure of the individuals to the low dosages from the I-DIR had led to the occurrence of some slight symptoms that can't be noticed via outside signs as in the high dosages, therefore, it we have studied the changes in gene expression like XRCC-I, XRCC-2, FOXM-I, it was researched hSSB1 (NABP2) for the purpose of knowing effects of the I-DIR. Using radioisotopes in industry, science, and medicine, must be implemented in such a way as to keep the environment and people safe for a long time.

Keywords: Oxidative stress; NABP2 (SSBI); Transcription factor; Biomarker; x-ray; Diagnosis; Reactive oxygen species (ROS) 


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References


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DOI: http://dx.doi.org/10.62940/als.v11i1.1945

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