In vitro evaluation of bacterial viability and adhesion of trio bacterial species on surface of titanium and zirconium dental implant abutment

Hanan Selman Hessan, Reyam Abdul Khuder Mohammed

Abstract


Background: Streptococcus mutans and Streptococcus sanguinis are two species of bacteria belonging to the Streptococcus genus. Both S. mutans and S. sanguinis are part of the natural oral microbiota, but their roles and impacts on oral health differ. While S. mutans is associated with tooth decay, S. sanguinis helps maintain oral health by preventing the colonization of harmful bacteria.

Methods: Two species of Streptococcus which are S. mutans and S. Sanguinis and the genus Porphyromonas gingivalis were evaluated for their adherence and viability in vitro on Titanium, zirconium and dental implant surfaces, in addition to their individual screw. Two research groups were designed; 3 anvils of titanium included in group 1 and 3 anvils of zirconium included in group 2. The above groups were filtered into tubes containing cultures of bacteria, S. mutans and S. sanguinis, as well as P. gingivalis separately . The incubation time under anaerobic and anaerobic conditions was set at 37 °C for 24 hr. The adjustment in the number of colony-forming units of bacteria was tested for bacterial adherence (CFU). colorimetric test (Methylene blue test) was used for bacterial viability evaluation. For S. mutans, bacterial adhesion was greater in the titanium abutments  (185.5 CFU/mL) and higher viability for P. gingivalis were published (71 % ).

Results: The results showed that S. mutans recorded the best overall adherence (330 CFU/mL), while the best overall viability recorded with S sanguinis, was demonstrated in the zirconium abutment community (36.4 % ). The greatest adhesion of S. sanguinis was demonstrated by the titanium screws (140.2 CFU/mL) . In contrast with the zirconium fixation screws,  the greatest adhesion (144.3 CFU/mL) was observed for  S. mutans. S. mutans recorded higher viability in both titanium and zirconium screws.

Conclusion: We may infer from this research that bacteria can bind to and thrive in both titanium and zirconium implants, as well as in fixation screws. S mutans demonstrated the strongest adherence to titanium and zirconium surfaces and fastening screws. In comparison, titanium abutments with P. gingivalis have greater bacterial viability than zirconium abutments with S. sanguinis. In both cases, as far as fixation screws are concerned, the feasibility of S. mutans  was higher  than the other bacteria. In titanium abutments greatest bacterial viability was recorded, while  less bacterial adherence.


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References


Llambés F, Arias-Herrera S, Caffesse R. Relationship between diabetes and periodontal infection. World journal of diabetes, (2015); 6(7): 927.

Thurnheer T, Paqué PN. Biofilm models to study the etiology and pathogenesis of oral diseases. Oral Biofilms, (2021); 2930-37.

Nyvad B, Takahashi N. Integrated hypothesis of dental caries and periodontal diseases. Journal of oral microbiology, (2020); 12(1): 1710953.

Nagashima H, Shinoda M, Honda K, Kamio N, Watanabe M, et al. CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice. Molecular Pain, (2017); 131744806916689269.

Sun Y, Yang Y, Jiang W, Bai H, Liu H, et al. In Vivo Antibacterial Efficacy of Nanopatterns on Titanium Implant Surface: A Systematic Review of the Literature. Antibiotics, (2021); 10(12): 1524.

Zhou N, Huang H, Liu H, Li Q, Yang G, et al. Microbiota analysis of peri-implant mucositis in patients with periodontitis history. Clinical Oral Investigations, (2022); 26(10): 6223-6233.

Genco RJ, Graziani F, Hasturk H. Effects of periodontal disease on glycemic control, complications, and incidence of diabetes mellitus. Periodontology, (2020); 83(1): 59-65.

Sgolastra F, Petrucci A, Severino M, Gatto R, Monaco A. Periodontitis, implant loss and peri‐implantitis. A meta‐analysis. Clinical oral implants research, (2015); 26(4): e8-e16.

Ardelean LC, Rusu LC. Oral Health Care-An Important Issue of the Modern Society. (2022).

Deppe H, Ahrens M, Behr AV, Marr C, Sculean A, et al. Thermal effect of a 445 nm diode laser on five dental implant systems: An in vitro study.

Scientific reports, (2021); 11(1): 20174.

Lee C-T, Huang Y-W, Zhu L, Weltman R. Prevalences of peri-implantitis and peri-implant mucositis: systematic review and meta-analysis. Journal of dentistry, (2017); 621-12.

Han A, Tsoi JK, Rodrigues FP, Leprince JG, Palin WM. Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors. International Journal of Adhesion and Adhesives, (2016); 6958-71.

Do Nascimento C, Pita MS, de Souza Santos E, Monesi N, Pedrazzi V, et al. Microbiome of titanium and zirconia dental implants abutments. Dental Materials, (2016); 32(1): 93-101.

Alvarado Pinedo MF (2022) Reconfiguración del diseño curricular del curso de Anatomía e Histología Básica para una nueva carrera de grado: desafíos de la enseñanza para un perfil profesional en construcción: Universidad Nacional de La Plata.

Caro-Hernández PA, Tobar JA. Análisis microbiológico de superficies en contacto con alimentos. Entramado, (2020); 16(1): 240-249.

Nga N, Ngoc T, Trinh N, Thuoc T, Thao D. Optimization and application of MTT assay in determining density of suspension cells. Analytical biochemistry, (2020); 610113937.

Zhurakivska K, Ciacci N, Troiano G, Caponio VCA, Scrascia R, et al. Nitride-Coated and Anodic-Oxidized Titanium Promote a Higher Fibroblast and Reduced Streptococcus gordonii Proliferation Compared to the Uncoated Titanium. Prosthesis, (2020); 2(4): 333-339.

Oda Y, Miura T, Mori G, Sasaki H, Ito T, et al. Adhesion of streptococci to titanium and zirconia. Plos one, (2020); 15(6): e0234524.

Alqutaibi AY, Alnazzawi AA, Algabri R, Aboalrejal AN, AbdElaziz MH. Clinical performance of single implant-supported ceramic and metal-ceramic crowns: A systematic review and meta-analysis of randomized clinical trials. The Journal of Prosthetic Dentistry, (2021); 126(3): 369-376.

Júnior RdAV, Aranha LC, Elias CN, Martinez EF. In vitro analysis of prosthetic abutment and angulable frictional implant interface adaptation: Mechanical and microbiological study. Journal of Biomechanics, (2021); 128110733.

Vinhas AS, Aroso C, Salazar F, López-Jarana P, Ríos-Santos JV, et al. Review of the mechanical behavior of different implant–abutment connections. International Journal of Environmental Research and Public Health, (2020); 17(22): 8685.


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