Comparison between some genera of Grammosciadium and Torilis belonging to the (Apiaceae) family using nucleotide sequence of Matk gene into a protein
Abstract
Background: The amino acid composition of certain species of the Grammosciadium and Torilis genera, belonging to the Apiaceae or Umbelliferae family, is investigated. This family of flowering plants is named after the genus Apium and is commonly referred to as the celery or carrot family. These plants are found in the wild in temperate regions and the northern regions of Iraq.
Methods: The research was carried out in the Molecular Genetics Laboratory at the College of Education for Pure Sciences, University of Diyala, Iraq. A total of 40 plant samples were collected from various places in Iraq, with 10 samples obtained for each species. The pliable specimens were enclosed in nylon pouches, stored in the refrigerator, and conveyed to the laboratory for the explicit objective of DNA extraction, with the subsequent data being duly documented. The molecular systematic research on Apioideae has involved international collaboration. The ongoing investigations will focus on analyzing the remaining 200 or so genera that have not been reviewed previously, particularly those that have been difficult to classify phylogenetically. Additionally, the current tribes and clades will be re-examined and improved. The current and upcoming updates to all of these well-known groups, which will include both molecular and morphological information, will finally lead to the creation of a new classification for the subfamily.
Results: The results demonstrated a distinction in nucleotide sequences of the Matk gene across two genera as well as between species belonging to the same genus. Specifically, there were variations in the arrangement of 20 amino acids. The pH value of 14.03 was recorded as the maximum for citric acid in G. daucoides.
Conclusion: The commonality lies in the relative quantities of certain amino acids. Members of the same genus, as well as people belonging to the same species.
Keywords: Grammosciadium daucoides; Grammosciadium platycarpum; Torilis arvensis; Torilis chrysocarpa; Apiaceae (Umbelliferae); Amino acids; Molecular (gene Matk)
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