Staphylococcus aureus isolated from various sites: Detection and diagnosis of some virulence factors production and antibiotic susceptibility

Morooj Ali Fahad, Nabaa Ali Jasim, Shahad Nazar Mustafa, Fatima Malallah Mohammed

Abstract


Background: Resistance of Staphylococcus aureus to commonly used antibiotics is linked to their ability to acquire and disseminate antimicrobial-resistant determinants in nature, and the marine environment may serve as a reservoir for antibiotic-resistant bacteria. Species of this genus can be distinguished by their capability to produce the coagulase enzyme that causes blood clotting, polypeptides that bind to activate prothrombin, in that way converting fibrinogen to fibrin and promoting the clotting of plasma or Blood Fifty skin swabs.

Methods: Samples were collected from patients who are burn and wound infections of Al-Yarmouk teaching hospital, during the period from November 2021 to March 2022, the researchers used different method to diagnosis of antibiotic resistance and alpha hemolysin assay by using a micro titer plate.

Results: The results were found by using cultural traits, microscopic analysis, and biochemical tests, twelve Staphylococcus aureus  isolates were discovered. A number of antibiotics, including ciprofloxacin/CIP, cefotaxime/CTX, gentamycin/CN, tetracycline/TE, and chloramphenicol/C), were tested using the disc diffusion technique on Staphylococcus aureus  isolates.

Conclusion: It was discovered that the ten samples had alpha toxin production activity and their susceptibility to antibiotics was known.


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References


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DOI: http://dx.doi.org/10.62940/als.v10i0.2002

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