Cytotoxic and Antioxidant Activity of Linseeds Against Rhabdomyosarcoma Cell Line

Dhifaf Zeki Aziz, Maysaa Adil Hadi, Khalida Kadhim Abbas Al-Kelaby, Zahraa Ali Abdullah, Azhar Hamza, Amel Ali Altaee, Hanan Ahmed Hadi AL-Qaraawi

Abstract


Background: Flaxseeds (Linum usitatissimum L.) are rich in phytochemicals and antioxidants that may have anticancer properties.   

Methods: The anticancer activity of the hydro-methanolic extract of linseeds (Linum usitatissimum  L.) on the Rhabdomyosarcoma (RD) cell line had been evaluated through determination of phytochemical content, anti-oxidative potential by the free. radical-scavenging capability of 2, 2, diphenyl_1_picrylhydrazyl (D.P.P.H) and cytotoxic effects which were carried out by utilizing M.T.T assay.

Results: Phytochemical analysis recorded various phytochemical compounds, with antioxidant activity of hydro-methanolic extract of linseeds. Furthermore, linseeds hydro-methanolic extract has a significant cytotoxic influence on the RD cell line in concentration ranged 0.05-80 mg/ml by MTT assay with an IC50 value of 0.07 mg/ml.

Conclusion: From the performed assays, the hydro-methanolic extract of linseeds shows greater activity on the RD cell line which ensured the possibility of applying linseeds as anticancer that due to the anti-oxidative potential of its phytochemical compounds.


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References


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DOI: http://dx.doi.org/10.62940/als.v10i0.2042

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