Genetic Relationships Among Local Oscillatoria Isolates from Freshwater Sources Using Inter-Simple Sequence Repeat (ISSR) Technique

Safa Ghassan Hameed, Harith Kamil Baniya

Abstract


Background: Molecular characteristics are good markers for distinguishing between closely related species. Molecular taxonomy has been significantly improved by DNA-based strategies with increasing accuracy and efficiency in identifying species through the use of PCR-based methods. We used inter-simple sequence repeat (ISSR) markers in this research to compare and examine the genetic profiles of several Oscillatoria species from native freshwater environments.

Methods: Algal samples were taken from natural freshwater environments in the autumn season (September–November 2022). To reduce contamination, DNA was obtained from unialgal cultures of Oscillatoria spp. Then, ISSR analysis was done to assess genetic diversity and phylogenetic relationships between the isolated strains.

Results: Seven species were identified: Oscillatoria acuta (OS1), O. princeps Vaucher (OS2), O. annae (OS3), O. margaritifera (OS4), O. proteus Skuja (OS5), Oscillatoria sp. (OS6), and O. sancta (OS7). O. annae and Oscillatoria sp. were most similar and were found to have the greatest genetic similarity (index = 0.6598), indicating that a close evolutionary relationship exists. The two with the lowest similarity were O. acuta and O. proteus Skuja (index = 0.4330), indicating greater genetic divergence.

Conclusion: Our results support the use of ISSR markers in determining genetic diversity and phylogenetic relationships among Oscillatoria spp. This method is promising for improving the molecular taxonomy of cyanobacteria.

Keywords: 

Genetic Diversity, Oscillatoria, Phylogenetic analysis, Cyanobacterial taxonomy, ISSR markers

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References


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DOI: https://doi.org/10.62940/als.v13i1.2194

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