Impact of varying sorbitol concentrations on hematological and biochemical blood parameters in Wistar rats (Rattus norvegicus)

Zahraa H.K. Al-Moussawi, Mozhir K.K. Almahdawi, Abdul Sattar S.S. Al-Bayati

Abstract


Background: Sorbitol is an artificial sweetener that is very similar to natural sugar, but it is sweeter than natural sugar and lower in calories. Sorbitol is used as an alternative to natural sugar, and it is extracted from regular glucose. It does not have any negative effects on the animals' health, and it is characterized in safe use in many scientific experiments for the diet of humans and animals.

Methods: This study was conducted in the animal house, College of Veterinary Medicine, Al-Qasim Green University for a period of 90 days. Forty rats of the Wistar strain (Rattus norvegicus) were randomly distributed into 4 groups, namely the first group (control group) while the second, third and fourth groups were treated with 100, 200 and 300 μg of sorbitol/kg of body weight .The rats were iso in live weight and age at the beginning of the experiment. Blood samples were drawn at the end of the experimental period.

Results: Increase in level dose of sorbitol resulted significant improvement in accounts of red blood cells, white blood cells, and a significant increase in level of hemoglobin, lymphocytes, and monocytes for second, third, and fourth groups compared to the control group. Moreover, the significant improvement in most of the blood parameters was reflected to significant increase in clotting and bleeding time in the second, third and fourth groups of rats compared to the control group. Also, the results showed a significant increase in the levels of FSH, LH, testosterone, Inhibin and MAD hormones for the second, third and fourth groups compared to the control group.

Conclusions: The results of the current study revealed that sorbitol has many benefits in the diet of humans and animals and has an effect on blood indicators, some sex hormones and antioxidants in the body. It is recommended not to consume excessive sorbitol in the diet.

Keywords: Sorbitol; Hematological parameters; FSH; LH; Wistar rats

Editorial Note on Version of Record
20 Oct 2026: This article has been corrected. See https://doi.org/10.62940/als.v13i0.4389 for more information.


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DOI: https://doi.org/10.62940/als.v11i4.2220

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