Screening and Optimization of Dextransucrase Production from Lactobacillus acidophilus

Yasameen Hasan Ali Al-anbakey, Sahira Nsayef Muslim, Israa Hussein Hamzah

Abstract


Background: Dextransucrase is an enzyme synthesized by many bacterial genera, which catalyzes the hydrolysis of sucrose to produce dextran, a polysaccharide with a wide range of medical, pharmaceutical, and industrial applications. The study aimed at isolating Lactobacillus species from the human vagina, assessing the production of dextransucrase, and determining the optimal growth conditions.

Methods: A total of 53 bacterial samples were isolated from the reproductive tract of healthy women. Out of these 53 samples, 21 isolates of Lactobacillus spp. were obtained. Dextransucrase production was assessed using both the mucoid and spectrophotometric techniques. The effects of natural carbon, nitrogen sources, concentrations of nitrogen, protein, and sucrose, inoculum size, pH, temperature, and incubation time were evaluated to determine the optimal conditions.

Results: The findings showing that Lactobacillus acidophilus isolates produced more dextransucrase than isolates of Lactobacillus plantarum, with Lactobacillus acidophilus V19 being the strongest producer isolate due to its highest specific activity. The best conditions for producing dextransucrase were to use a dextransucrase production medium that underwent incubation at 37°C for 48 hours at pH 5.5, with a concentration of 6% sucrose, and 4% inoculum size. The most efficient source of nitrogen was 6% beef extract, and the best source of carbon was dates. At this point, the dextransucrase activity was at its highest.

Conclusion: The findings of the study revealed that the optimal conditions for dextransucrase production are a pH of 5.5, 4% inoculum size, 6% dates extract as a carbon source, 6% beef extract as a nitrogen source, and 48 hours of incubation at 37°C.

Keywords: Ecological; Optimization; Dextransucrase; Lactobacillus acidophilus  


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References


Mahmood NN, Hameed AA. Probiotic activity of Lactobacillus spp. from vaginal specimens against bacterial pathogens. Journal of University of Babylon for Pure and Applied Sciences, (2018); 26(5): 335-346.

Ozen M, Piloquet H, Schaubeck M. Limosilactobacillusfermentum CECT5716: Clinical Potential of a Probiotic Strain Isolated from Human Milk. Nutrients, (2023): 15(9), 2207.

Smith PJ, Ortiz-Soto ME, Roth C, Barnes WJ, Seibel J, Urbanowicz BR, Pfrengle F. ACS Sustainable ChemistryanD Engineering, (2020): 8(32): 11853–11871.

Mu D, Zhou Y, Wu X, Montalban-Lopez M, Wang L, Li X, Zheng, Z. Secretion of Bacillus amyloliquefacienslevansucrase from Bacillus subtilis and its application in the enzymatic synthesis of l evan. ACS Food Science and Technology, (2021); 1(2): 249-259.

Vuillemin M, Grimaud F, Claverie M, Rolland-Sabaté A, Garnier C, Lucas P, Monsan P, Dols-Lafargue M, Remaud-Siméon M, Moulis C.. A dextran with unique rheological properties produced by the dextransucrase from Oenococcuskitaharae DSM 17330. Carbohydrate Polymers, (2018); 179: 10-18.‏

Besrour-Aouam N, Fhoula I, Hernández-Alcántara AM, Mohedano ML, Najjari A, Prieto A, Ruas-Madiedo P, López P, OuzariHI.. The role of dextran production in the metabolic context of Leuconostoc and Weissella Tunisian strains. Carbohydrate Polymers, (2021); 253: 117254.‏

Guzman GYF, Hurtado GB, Ospina SA. New dextransucrase purification process of the enzyme produced by Leuconostocmesenteroides IBUN 91.2. 98 based on binding product and dextranase hydrolysis. Journal of biotechnology, (2018); 265: 8-14.‏

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, (1976); 72(1-2): 248-254.

Prechtl RM, Janßen D, Behr J, Ludwig C, Küster B, Vogel RF, Jakob F. Sucrose-induced proteomic response and carbohydrate utilization of Lactobacillus sakei TMW 1.411 during dextran formation. Frontiers in Microbiology, (2018): 9: 2796.‏

Guérin M, Silva CRD, Garcia C, Remize F. Lactic acid bacterial production of exopolysaccharides from fruit and vegetables and associated benefits. Fermentation, (2020); 6(4): 115.

Hosaka H, Kawamura M, Hirano T, Hakamata W, Nishio T. Utilization of sucrose and analog disaccharides by human intestinal bifid bacteria and lactobacilli: search of the bifid bacteria enzymes involved in the degradation of these disaccharides. Microbiological Research, (2020); 240: 126558.‏

YasawyMI. The unexpected truth about dates and hypoglycemia. Journal of Family snd Community Medicine, (2016); 23(2): 115–118.

Suribabu K, Hemalatha KPJ. Thermal Optimization of α-amylase Production in Brevibacillus sp. International Journal of Current Microbiology and Applied Sciences, (2016); 5(3): 909-916.

Srinivas B, Padma PN. Screening of diverse organic, inorganic and natural nitrogen sources for dextran production by WeissellaSpp using Plackett-Burman design. International Journal of Scince and Technology Reseatch, (2014); 3(4): 319-328.‏

Koskar J, Meremäe K, Püssa T, Anton D, Elias T, Rätsep R, Mäesaar M, Kapp K, Roasto M. Microbial Growth Dynamics in Minced Meat Enriched with Plant Powders. Applied Sciences, (2022); 12(21): 11292.‏

Thi Nguyen HY, Tran GB. Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology. Scientifica, (2018); 2018: 6842843.

Robinson PK. Enzymes: principles and biotechnological applications. Essays in Biochemistry, (2015);59:1-41.

Krizsan SJ, Huhtanen P. Effect of diet composition and incubation time on feed indigestible neutral detergent fiber concentration in dairy cows. Journal of Dairy Science, (2013); 96(3): 1715-1726.


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