Molecular detection and probiotic treatment for bacteria that cause diarrhea

Riyam Mohsen Dakheel Al-Hilali, Hanaa Daaj Khalaf Al-Mozan

Abstract


Background: Misdiagnosis of the pathogen leads to serious consequences. Diarrhea is a worldwide health problem that kills 1.3 million people each year. Diarrhea can be induced by a variety of causes. However, infectious agents like bacteria are among the leading causes. Several resistant bacterial strains are emerging that tend to spread globally; hence, resistance of bacteria to antibiotics is a global health threat. The study aimed to ascertain the Saccharomyces boulardii filter's impact on lowering the virulence of the bacterial species that cause diarrhea and the extent of genetic affinity between those organisms.

Methods:  350 stool samples were investigated for all age groups who suffer from diarrhea, using biochemical and molecular methods.

Results: Biochemical diagnosis were 2.28% for Salmonella and 0.57% for Enterobacter aerogenes, while  molecular diagnosis didn’t record any percentage for the mentioned bacteria, therefore molecular test was a better way to properly diagnose bacterial species, the causes of diarrhea are bacteria, viruses, parasites, medications, and toxins. The molecular identification of isolates by PCR technique and sequencing of 16S rDNA gene indicated that 12 isolates belonged to the genera Enterobacter cloacae, Cronobacter sakazakii, and Aeromonas hydrophila. The study revealed that there is a difference in the rates of resistance of isolates to some antibiotics before and after exposing them to probiotics. Where the percentage of antibiotic resistance was recorded before exposure to probiotics were piperacilline (91.6%), cefotaxime/clavulanic acid (41.6%), and tetracycline (25%), while the percentage of antibiotic resistance after exposure to probiotics were (58.3%) piperacillin, (0%) cefotaxime/clavulanic acid, and (0%) tetracycline. Where the resistance of bacteria to antibiotics decreases after exposing them to probiotics.

Conclusion: The molecular diagnostic method is more accurate method to diagnose the bacterial species with complete accuracy. The Saccharomyces boulardii filter had an effective role in reducing or suppressing bacterial resistance against piperacillin, cefotaxime/clavulanic acid, and tetracycline, while it did not affect the bacterial resistance towards amoxicillin clavulanic acid.


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References


Li Y, Xia S, Jiang X, Feng C, Gong S, Ma J, Fang Z, Yin J, Yin Y. Gut microbiota and diarrhea: an updated review. Frontiers in cellular and infection microbiology, (2021); 11: 625210.

Beyene AM, Gezachew M, Mengesha D, Yousef A, Gelaw B. Prevalence and drug resistance patterns of Gram-negative enteric bacterial pathogens from diarrheic patients in Ethiopia: A systematic review and meta-analysis. Plos one, (2022); 17(3): e0265271.

Shahi SK, Zarei K, Guseva NV, Mangalam AK. Microbiota analysis using two-step PCR and next-generation 16S rRNA gene sequencing. JoVE (Journal of Visualized Experiments), (2019); (152):e59980.

Li H, Zhong Q, Wirth S, Wang W, Hao Y, Wu S, Zou H, Li W, Wang G. Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (C tenopharyngodon idellus)(V alenciennes) determined by culture‐dependent and culture‐independent techniques. Aquaculture Research, (2015); 46(10): 2344-59.

Srinivasan R, Karaoz U, Volegova M, MacKichan J, Kato-Maeda M, Miller S, Nadarajan R, Brodie EL, Lynch SV. Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens. PloS one, (2015); 10(2): e0117617.

Kato H, Hamada Y. Amikacin Therapy in Japanese Pediatric Patients: Narrative Review. International Journal of Environmental Research and Public Health, (2022); 19(4): 1972.

Idea SC, Nazar B, Hall S. Role of Probiotics Saccharomyces Boulardii in The Management of Acute Watery Diarrheas in Children. Journal of Medicine, Physiology and Biophysics, (2018); 43: 81-84.

Al-zaidi RE, Al-Mozan HD, Alrikabi NJ. Eukaryotic probiotic Saccharomyces boulardii application in clinical trails: a review. International Journal of Pharmaceutical Quality Assurance. 2020;11(1):163-8.

Atıcı S, Soysal A, Cerit KK, Yılmaz Ş, Aksu B, Kıyan G, Bakır M. Catheter-related Saccharomyces cerevisiae Fungemia Following Saccharomyces boulardii Probiotic Treatment: In a child in intensive care unit and review of the literature. Medical mycology case reports, (2017); 15: 33-5.

AL-Lami WA, Mezal EH. Detection some Antimicrobial Resistance Genes in Salmonella sp. Isolated from Chicken Meat. University of Thi-Qar Journal of Science, (2021); 8(1): 90-100.

Hussein KR, Al-Temimy N, Widaa QH, Nasser MH. Eschershia coli integrons and cfr, procfr genes identified from samples of autistic children's stools. University of Thi-Qar Journal of Science, (2023); 10(1 (SI)): 1020.

Miyoshi T, Iwatsuki T, Naganuma T. Phylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometer-pore-size filters. Applied and environmental microbiology, (2005); 71(2): 1084-8.

ALYOUSIF NA. Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq. Biodiversitas Journal of Biological Diversity, (2022); 23(2): 679-686.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, (2018); 35(6): 1547.

Humphries R, Bobenchik AM, Hindler JA, Schuetz AN. Overview of changes to the clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing, M100. Journal of clinical microbiology, (2021); 59(12): 10-128.

Qazangi,M.O.M. Practical experiments in microbiology. 1th Edition, Iraq, University of Baghdad – Science of Agriculture, (2017): 1-138.

Al-Mozan, H.D.K. Molecular detection of enterotoxins genes in Staphylococcus aureus isolated from clinical and dairy samples. A thesis submitted to the college of science/ University of Basrah, (2021):1-131.

khalaf Al-aawadi K. Phenotypic and Molecular Identification of Cronobacter sakazakii Isolated from Clinical Specimens in Thi-Qar Province. University of Thi-Qar Journal of Science, (2022); 9(1): 62-7.

Qin, X., Yang, M., Cai, H., Liu, Y., Gorris, L., Aslam, M.Z., Jia, K., Sun, T., Wang, X. and Dong, Q., 2022. Antibiotic resistance of Salmonella Typhimurium monophasic variant 1, 4,[5], 12: i:-in China: a systematic review and meta-analysis. Antibiotics, 11(4), p.532.

Abid R, Waseem H, Ali J, Ghazanfar S, Muhammad Ali G, Elasbali AM, Alharethi SH. Probiotic yeast Saccharomyces: Back to nature to improve human health. Journal of Fungi, (2022); 8(5): 444.

Czerucka D, Piche T, Rampal P. yeast as probiotics–Saccharomyces boulardii. Alimentary pharmacology & therapeutics, (2007); 26(6): 767-78.

Pais P, Almeida V, Yılmaz M, Teixeira MC. Saccharomyces boulardii: what makes it tick as successful probiotic?. Journal of Fungi, (2020); 6(2): 78.




DOI: http://dx.doi.org/10.62940/als.v10i0.2320

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