Developing a Nano Platform for Bovine Brucellosis Diagnostic Product

Gulmira Tulepova, Edil Makhashov, Loukia Ekateriniadou, Nurzhan Nurkhojayev, Sagypash Sadiev

Abstract


Background: Brucellosis is one of the most common zoonoses caused by Brucella species. Brucella is an intracellular pathogen that causes abortion in domestic animals and brucellosis in humans. The disease has no specific pathognomonic signs; hence a prompt and accurate diagnosis of the disease plays a very critical role in preventing brucella transmission and treating the disease.

Methods: Research was carried out at the Department of Veterinary Science of Kazakh National Agrarian Research University and in the Molecular Biology laboratory of the Institute for Plant Biology and Biotechnology under the Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan. Studies were carried out on a killed culture of the vaccine strain Brucella abortus RB19, genomic DNA of B. abortus RB19, Grapevine virus A, plasmid vectors for DNA cloning, a fluorescent nano marker, and Brucella membrane proteins.

Results: The research demonstrated that brucellosis remained one of the most common zoonotic diseases worldwide. This disease not only resulted in significant economic losses in agriculture but also posed a serious threat to public health.

Conclusions: The study successfully established prokaryotic expression vectors for efficient protein production, validated through sequencing and Western blotting, and proposed a promising nano-platform for plant-based synthesis of brucellosis proteins, with potential implications for diagnostic kit development and vaccine research in agriculture.

Keywords: Brucellosis; Brucella; Outer membrane proteins; Omp16  


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References


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DOI: http://dx.doi.org/10.62940/als.v11i2.3191

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