Association of Celiac Disease and H. pylori Infection with ATG5 Polymorphism and Interleukin-33
Abstract
Background: Celiac disease (CD) is an inflammatory small intestine autoimmune disease. The study aims to investigate the association and the detection of ATG5 polymorphism between celiac disease (CD) and H. pylori infection, and the association with Interleukin-33 (IL-33).
Methods: The study groups included patients from Iraq, ages 4 to 35. Two primary patient groups were created: sixteen had positive H. pylori and celiac disease, and thirty had positive CD and negative H. pylori disease. The levels of tissue transglutaminase IgA (TTG IgA), H. pylori IgG, and IL-33 were measured using the ELISA method. The primers were amplified using PCR.
Results: With celiac disease, the patient group's TTG IgA levels increased dramatically. The test also showed significant variations (P=0.054) in the H. pylori IgG levels between the patient and control groups. The H. pylori seropositivity test showed a statistically significant difference (p ≤0.033) between seropositive and seronegative individuals, while the patient group's IL-33 levels did not significantly differ (P ≤0.299) from the control group.
Conclusions: Our results showed that CD is more common in women and occurs in the age range of 24-35 years. It also showed that the mutant variant of ATG5 is associated with CD, and the significance of H. pylori IgG serum levels in the patient group may indicate that the bacteria involved in CD. Furthermore, H. pylori infection is more strongly linked to serum IL-33 levels than CD.
Keywords: Celiac disease; H. pylori; PCR; IL-33; ATG5 polymorphism
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Serena G, Lima R, Fasano A. Genetic and environmental contributors to celiac disease. Current Allergy and Asthma Reports, (2019); 19(9): 1-10.
Tokgöz Y, Terlemez S, Karul A. Fat-soluble vitamin levels in children with newly diagnosed celiac disease, a case-control study. BMC Pediatrics, (2018); 18(1): 1-5.
Lionetti E, Catassi C. New clues in celiac disease epidemiology, pathogenesis, clinical manifestations, and treatment. International reviews of immunology, (2011); 30(4): 219-231.
Abu-Zekry M, Kryszak D, Diab M, Catassi CF. Prevalence of celiac disease in Egyptian children disputes the east-west agriculture-dependent spread of the disease. Journal of pediatric gastroenterology and nutrition, (2008); 47(2): 136-140.
Ertekin V, Selimoglu MA, Kardas F, Aktas E. Prevalence of celiac disease in Turkish children. Journal of clinical gastroenterology, (2005); 39(8): 689-691.
Tye-Din JA, Galipeau HJ, Agardh D. Celiac disease: a review of current concepts in pathogenesis, prevention, and novel therapies. Frontiers in pediatrics, (2018); 6: 350.
Guevara B, Cogdill AG. Helicobacter pylori: a review of current diagnostic and management strategies. Digestive Diseases and Sciences, (2020); 65(7): 1917-1931.
Crowe SE. Helicobacter pylori infection. New England Journal of Medicine, (2019); 380(12): 1158-1165.
Ge Y, Huang M, Yao YM. Autophagy and proinflammatory cytokines: interactions and clinical implications. Cytokine & growth factor reviews, (2018); 43: 38-46.
Nikseresht M, Shahverdi M, Dehghani M, Abidi H, Mahmoudi R, et al. Association of single nucleotide autophagy‐related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer. Journal of Cellular Biochemistry, (2019); 120(2): 1924-1931.
Ye X, Zhou XJ, Zhang H. Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Frontiers in immunology, (2018); 9: 2334.
van Beek N, Klionsky DJ, Reggiori F. Genetic aberrations in macroautophagy genes leading to diseases. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, (2018); 1865(5): 803-816.
Zhang Y, He X, Li J, Yang W, Cui Y, et al. Functional genetic variant in ATG5 gene promoter in acute myocardial infarction. Cardiology Research and Practice, (2020); 2020 (1): 9898301.
Perez F, Ruera CN, Miculan E, Carasi P, Dubois-Camacho K, et al. IL-33 alarmin and its active proinflammatory fragments are released in small intestine in celiac disease. Frontiers in immunology, (2020); 11: 581445.
Cayrol C, Girard JP. Interleukin‐33 (IL‐33): a nuclear cytokine from the IL‐1 family. Immunological reviews, (2018); 281(1): 154-168.
Valitutti F, Cucchiara S, Fasano A. Celiac disease and the microbiome. Nutrients, (2019); 11(10):2403.
Khatoon S, Ahmed A, Yousaf S. Iron deficiency anemia in Pakistan: Celiac disease an underlying cause. Journal of Ayub Medical College Abbottabad, (2018); 30(3): 372-376.
Dickerson JA, Lee D, Pacheco MC. Deamidated gliadin peptide in pediatric patients with moderately increased tissue transglutaminase; does it help? Clinica Chimica Acta, (2019); 492: 20-22.
Khan A, Ahmad W, Kamran M. Celiac Disease in Different Age Groups and Genders in Pakistan. Journal of Rawalpindi Medical College, (2018); 22(3): 244-247.
Abdullah H, Thawani A. Association of celiac disease with HLA-DRB1 and HLADQB1 alleles in a sample of Iraqi patients. Iraqi Journal of Biotechnology, (2012); 11: 529-36.
Bayrak NA, Tutar E, Volkan B, Sahin AB, Polat E, et al. Helicobacter pylori infection in children with celiac disease: Multi‐center, cross‐sectional study. Helicobacter, (2020); 25(3): e12691.
Simondi D, Ribaldone DG, Bonagura GA, Foi S, Sapone N, et al. Helicobacter pylori in celiac disease and in duodenal intraepithelial lymphocytosis: Active protagonist or innocent bystander? Clinics and Research in Hepatology and Gastroenterology, (2015); 39(6):740–745.
Obaidat MM, Roess AA. First nationwide seroepidemiology and risk factors report of Helicobacter pylori in Jordan. Helicobacter, (2019); 24(3): e12572.
Abass EA. Relationship Between Interleukin-33 (IL-33) and C-Reactive Protein in Iraqi Women Patients with Celiac Disease. Ibn AL-Haitham Journal for Pure and Applied Science, (2017); 27(1): 288-296.
López-Casado MA, Lorite P, Palomeque T, Torres MI. Potential role of the IL-33/ST2 axis in celiac disease. Cellular & molecular immunology, (2017); 14(3): 285-292.
da Silva EA, da Silva NM, Rodrigues RR, Adad SJ, de Lima PS, et al. Arginase-1 and Treg profile appear to modulate inflammatory process in patients with chronic gastritis: IL-33 may be the alarm cytokine in H. pylori-positive patients. Mediators of Inflammation, (2019); 2019 (1): 25336781.
Li NF, Wang X, Deng H, Zhang K, Zhang X, et al. Autophagy-related 5 gene rs510432 polymorphism is associated with hepatocellular carcinoma in patients with chronic hepatitis B virus infection. Immunological Investigations, (2019); 48(4): 378-391.
Shao Y, Chen F, Chen Y, Zhang W, Lin Y, et al. Association between genetic polymorphisms in the autophagy-related 5 gene promoter and the risk of sepsis. Scientific reports, (2017); 7(1): 9399.
DOI: http://dx.doi.org/10.62940/als.v11i4.3267
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