GENETIC DIVERSITY AND PHYLOGENETIC RELATIONSHIP IN DIFFERENT GENOTYPES OF COTTON FOR FUTURE BREEDING
Abstract
Back ground: To make the plants well adapted and more resistant to diseases and other environmental stresses there is always a need to improve the quality of plant’s genome i.e. to increase its genetic diversity.
Methods: In the present study six variety and six lines of cotton were investigated for their genetic diversity and phylogenetic relationship. For this purpose 35 different RAPD primers obtained from the Gene Link Technologies,USA were used.
Results: Among 35 RAPD primers, 13 primers produced reproducible PCR bands while the rest failed to show any amplification product. Our results indicated that the total count of the reproducible bands was 670 and polymorphic loci were counted to be 442 which constitute 66% of total loci. Phylogenetic analysis revealed two major groups each consists of 7 and 5 genotypes respectively. Genotypes Lp1 and Tp4 were placed at maximum genetic distance and in separate groups and could be utilized for future cotton breeding.
Conclusions: RAPD analysis is a cheaper and time saving technique for the determination of genetic diversity of different cotton genotypes. Cotton genotype Lp1 and Tp4 could be the best candidates for future breeding programs as both genotypes are genetically distant from each other.
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Hussain A, Ibrahim M, Tajammal MA, Naz MA. Cotton Varieties of Pakistan. Federal Seed Certification and Registration Department. Ministry of Food, Agriculture &Livestock (MINFAL), Government of Pakistan, 2005.
Abdukarimov AS, Djataev S, Abdukarimov I. Cotton research in Uzbekistan: Elite cultivars and future cotton breeding. Proceedings World Cotton Research Conference Cape Town, South Africa, 2003; Pp. 5-15.
Ali AM, Ahmed OM, Misaka BC, Latif AH, Elsiddig K, Babiker EA. Characterisation of cotton germplasm and its utilization in breeding for major production constraints in Sudan. Proceedings World Cotton Research Conference Cape Town, South Africa, 2003; Pp. 16-21.
Asif M, Rahman MA, Mirza JI, Zafar Y. Parentage confirmation of cotton hybrids using molecular markers. Pakistan Journal of Botany, (2009); 41: 695-701
Bakht J, Fareed A, Shafi M, Swati ZA. 2011 a. Genetic diversity of NURYT-national Uniform Rape Seed Yield Trial and Brassica napus varieties using RAPD markers and biochemical analysis. Pakistan Journal of Botany, (2011a) 43: 2075-2081.
Bakht J, Ghaffar M, Shafi M, Latif A, Khan S. Determination of genetic diversity of different barley genotypes grown in Khyber Pakhtun Khwa using RAPD markers. Pakistan Journal of Botany, (2011b) 43: 2491-2495.
Rana MK, Singh VP, Bha KV. Assessment of genetic diversity in upland cotton (Gossypium hirsutum L.) breeding lines by using amplified fragment length polymorphism (AFLP) markers and morphological characteristics. Genetic Resources and Crop Evoluation, (2005) 52: 989-997.
Lacape JM, Dessauw D, Rajab M, Noyer JL, Hau B. Microsatellite diversity in tetraploid Gossypium germplasm: assembling a highly informative genotyping set of cotton SSRs. Molecular Breeding, (2007) 19: 45-58.
Mahmood Z, Raheel F, Dasti AA, Shahzadi S, Athar M, Qayyum M. Genetic diversity analysis of the species of Gossypium by using RAPD markers. African Journal of Biotechnology, (2009), 8: 3691-3697.
Khan AI, Awan FS, Sadia B, Rana RM, Khan IA. 2010. Genetic diversity studies among colored cotton genotypes by using RAPD markers. Pakistan Journal of Botany, (2010), 42: 71-77.
Khan AI, Khan IA, Awan FS, Sadaqat HA, Bahadur S. Estimation of genetic distance based on RAPD between 11 cotton accessions varying in heat tolerance. Genetic and Moelcualr Research, (2011), 10: 96-101.
Lin Z, He D, Zhang X, Nie Y, Guo X, Feng C, Stewart JM. Linkage map construction and mapping QTL for cotton fibre quality using SRAP, SSR and RAPD. Plant Breeding, (2005), 124: 180-187.
Devos KM, Gale MD. The use of Random Amplified polymorphic DNA markers in wheat. Theoretical and Applied Genetics, (1992) 84: 567-572.
Ni M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the national Academy of Sciences USA, (1979), 76: 5269-5276.
Hadrys H, Balik M, Schierwater B. 1992. Application of Random Amplified Polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology, (1992), 1: 55-60.
Mumtaz AS, Naveed M, Shinwari ZK. 2010. Assessment of genetic diversity and germination pattern in selected cotton genotypes of Pakistan. Pakistan Journal of Botany, (2010), 42: 3949-3956.
Ali MA, Seyal MT, Awan SI, Niaz S, Ali S, Abbas A. Hybrid authentication in upland cotton through RAPD analysis. Australian Journal of Crop Sciences, (2008), 2: 141-149.
Bakht J, Nazma N, Shafi M. Genetic diversity of different peaches cultivars and genotypes through RAPD primers. Pakistan Journal of Botany, (2012) 44: 1527-1532.
Perez-Artes E, Garcia-Pedrajas MD, Bejarano-Alcazar J, M.Jimenez-Diaz R. Differenciation of cotton-defoliating and Non-defoliating pathotypes of Verticillium dahlia by RAPD and specific PCR analysis. European Journal of Plant Pathology, (2000), 106: 507-517.
Lu HJ, Myers GO. 2002. Genetic relationships and discrimination of ten influential Upland cotton varieties using RAPD markers. Theoretical and Applied Genetics, 105: 325-331.
Rahman MM, Hussain D, Zafar Y. Estimation of genetic divergence among elite cotton cultivars-genotypes by DNA fingerprinting technology. Crop Science, (2002) 42: 2137-2144.
Kumar P, Singh K, Vikal Y, Randhawa LS, Chahal GS. Genetic diversity studies of elite cotton germplasm lines using RAPD markers and morphological characteristics. Indian Journal of Genetic and Plant Breeding, (2003) 63: 5-10.
Niu C, Hinchliffe DG, Cantrell RG, Wang C, Roberts PA, Zhang J. Identification of molecular markers associated with Root-Knot Nematode Resistance in upland cotton. Crop Sciences, (2007), 47: 951-960.
Esmail RM, Zhang JF, Abdel-Hamid AM. Genetic diversity in elite cotton germplasm lines using field performance and RAPD markers. World Journal of Agricultural Science, (2008), 4: 369-375.
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