Potential DNA Barcoding for Identification of Large Leaf Homalomena (Homalomena pendula (Blume) Bakh.f.)

Le Nguyen Thoi Trung, Hoang Tan Quang, Truong Thi Bich Phuong, Tran Nam Thang

Abstract


Background: Homalomena is a genus of the Araceae family, which consists of many medicinal plants used in Vietnamese traditional medicine. However, this genus shows relatively similar morphological characteristics across its species, so classification by morphological comparison has several limitations, and the scientific names and classifications of some species remain controversial. H. pendula is an endangered medicinal herb, highly valued for its tonic, analgesic, and anti-inflammatory properties, but wild populations are threatened by overharvesting. In addition, the DNA barcodes for H. pendula have not been well studied. Thus, developing DNA barcodes for H. pendula is necessary to identify and conserve this species.

Methods: This study used four DNA barcodes (trnL-trnF, rbcL, trnH-psbA, and trnQ-rps16) to determine the most effective DNA barcode sequences for distinguishing H. pendula.

Results:  Among 4 Homalomena species, one SNP was found in the trnL-trnF sequence at position 16 (A > G), and three SNPs were found in the rbcL sequence at positions 126 (C > T), 272 (G > A), and 278 (T > A). Five different H. pendula samples collected from Hue city, Vietnam, distributed in a separate branch from trnL-trnF or rbcL on the phylogenetic tree. Both trnH-psbA and trnQ-rps16 fragments have lots of changes in nucleotide sequences, however, these are random differences and are not significant in H. pendula identification, only useful for genetic diversity studies.

Conclusion: Based on the nucleotide sequences and phylogenetic tree analysis, the results show that trnL-trnF and rbcL markers can be used to distinguish H. pendula from other closely related species.

Keywords: Chloroplast, DNA Barcode, Homalomena pendula, Phylogeny, Natural Forest, Vietnam


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References


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DOI: https://doi.org/10.62940/als.v13i2.3932

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