Hemoglobin adducts in paint industry workers: An electrophoretic analysis

Sumera Qureshi, Sikander Ali Memon, Allah Bux Ghanghro, Muhammad Faisal Qureshi, Moina Akhtar Mughal, Tahira Qureshi


Background: Hemoglobin (Hb) has a significant role among other blood proteins vital for carrying nutrients to blood cells. Being a conjugated protein, Hb is prone to be captured by compounds of low molecular weight like organic acid anhydrides (OAAs) which are prominent industrial/occupational hazards. Hindered or lowered availability of Hb to blood cells can cause anemia, thalassemia and porphyria. Along with these disorders, workers exposed to OAAs can also acquire like type-I allergy, type-IV allergy, skin problems, rhinitis and asthma. Revelation of Hb-OAAs compounds prior to appearance of actual symptoms could be important for subsequent therapy.

Methods: The Hb separation was achieved successfully by SDS-PAGE electrophoresis on 10-15% gels of different concentration, stained with CBB-R250 Blue. Total of 66 blood protein samples were used for the comparative study of exposed workers of paint industry workers with control (normal) group to detect proteins, which might serve as marker for the early disease diagnosis.

Results: The better Hb separation resolution was achieved on 12% gel as shown in electrograms. The electrograms of paint workers exposed to OAAs showed bands at 12, 48, 66, 78, 128 and 132 KDa in most of cases. In normal cases the bands were found at 13, 30, 48, 67, 76, 125 and 155 KDa in majority of control samples for Hb electrophoresis.

Conclusion: This study supports the association between Hb and OAAs adducts among the exposed paint workers from hypersensitive effects like fever (rhinitis) leading to asthma, skin allergies and major clinical effects.


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Kakar S, Hoffman FG, Storz JF, Fabian M, Hargrove MS. Structure and reactivity of hexacoordinate hemoglobins. Biophysical chemistry, (2010); 152(1): 1-14.

Amiri M, Farzin L, Moassesi ME, Sajadi F. Serum trace element levels in febrile convulsion. Biological trace element research, (2010); 135(1-3): 38-44.

Bianco L, Unger E, Beard J (2010) Iron deficiency and neuropharmacology. Iron Deficiency and Overload: Springer. pp. 141-158.

Baker RD, Greer FR. Diagnosis and prevention of iron deficiency and iron-deficiency anemia in infants and young children (0–3 years of age). Pediatrics, (2010); 126(5): 1040-1050.

Huma N, Salim-Ur-Rehman, Anjum FM, Murtaza MA, Sheikh MA. Food fortification strategy—preventing iron deficiency anemia: a review. Critical reviews in food science and nutrition, (2007); 47(3): 259-265.

Beard JL. Iron biology in immune function, muscle metabolism and neuronal functioning. The Journal of nutrition, (2001); 131(2): 568S-580S.

Kristiansson MH, Jönsson BA, Lindh CH. Mass spectrometric characterization of human hemoglobin adducts formed in vitro by hexahydrophthalic anhydride. Chemical research in toxicology, (2002); 15(4): 562-569.

Richter E, Branner B. Biomonitoring of exposure to aromatic amines: haemoglobin adducts in humans. Journal of Chromatography B, (2002); 778(1): 49-62.

Rosqvist S, Johannesson G, Lindh CH, Jönsson BA. Total plasma protein adducts of allergenic hexahydrophthalic and methylhexahydrophthalic anhydrides as biomarkers of long-term exposure. Scandinavian journal of work, environment & health, (2001); 133-139.

Zhang X-D, Welinder H, Jönsson BA, Skerfving S. Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing. Scandinavian journal of work, environment & health, (1998); 220-227.

Nielsen J, Welinder H, Jönsson B, Axmon A, Rylander L, et al. Exposure to hexahydrophthalic and methylhexahydrophthalic anhydrides—dose-response for sensitization and airway effects. Scandinavian journal of work, environment & health, (2001); 327-334.

Nielsen J, Welinder H, Bensryd I, Rylander L, Skerfving S. Ocular and airway symptoms related to organic acid anhydride exposure–a prospective study. Allergy, (2006); 61(6): 743-749.

Wernfors M, Nielsen J, Schütz A, Skerfving S. Phthalic anhydride-induced occupational asthma. International Archives of Allergy and Immunology, (1986); 79(1): 77-82.

Venables KM. Low molecular weight chemicals, hypersensitivity, and direct toxicity: the acid anhydrides. British journal of industrial medicine, (1989); 46(4): 222-232.

Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry, (1977); 252(3): 1102-1106.


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