Hepatoprotective role of fruit extract of Terminalia arjuna in acetaminophen intoxicated mice

Saira Khan, Faiza Noor, Imran Sohail, Sarmad Imtiaz, Faiza Anum, Sana Sarmad, Shaoib Kabir, Shahid Raza


Background: Liver injury and dysfunction is one of the major health concerns throughout the world. Several herbal formulations are reported to exert beneficial effects on the biochemistry of the liver.

Methods: Therefore, the current project is conducted to evaluate the hepatocurative and hepatoprotective potential of Terminalia arjuna by using albino mice.

Results: The fruit extract (400 mg/Kg) of the plant showed hepatoprotective effects upon pre-treatment for 5, 10 and 15 days and later challenged with acetaminophen (400 mg/Kg) for 3 days. The results showed substantial protective properties as there was comparatively less damage to the liver. Furthermore, the fruit extract of T. arjuna also exhibited hepatocurative effects when animals were given acetaminophen (400 mg/Kg) for 3 days to damage the liver followed by the treatment with the plant extract (400 mg/Kg) for 5, 10 and 15 days. The results also indicated hepatocurative activities, as the elevated serum levels of hepatic enzymes were inclining to normal ranges in a time-dependent manner.

Conclusion: In conclusion, the fruit extract of T. arjuna possesses hepatoprotective plus hepatocurative activities.

Keywords: Acetaminophen; Hepatocurative; Hepatoprotective; Serum Enzymes; Terminalia arjuna

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Abbas Z, Khan SM, Alam J, Khan SW, Abbasi AM. Medicinal plants used by inhabitants of the Shigar Valley, Baltistan region of Karakorum range-Pakistan. Journal of Ethnobiology and Ethnomedicine, (2017); 13: 53.

Ali BH, Bashir AK, Rasheed RA. Effect of tranditional medicinal plants Rhazystricta, Balanitisalgyptiara and Haplophylumtuberculatum on paracetamol induced hepatotoxicity in mice. Phytotherapy Research, (2001); 15: 598-603.

Amal raj A, Gopi S. Medicinal properties of Terminalia arjuna (Roxb.) Wight &Arn.: a review. Journal of Traditional and Complementary Medicine , (2017); 7(1): 65-78.

Bishop S, Liu SJ. Cardioprotective action of the aqueous extract of Terminalia arjuna bark against toxicity induced by doxorubicin. Phytomedicine, (2017); 36: 210-216.

Biswas M, Karan TK, Kar B, Bhattacharya S, Ghosh AK, Kumar RBS, Haldar PK. Hepatoprotective activity of Terminlia arjuna leaf against paracetamol induced liver damage in rats. Asian Journal of Chemistry., (2011); 23:1739-1742.

Chaudhari GM, Mahajan RT. In vitro hepatoprotective activity of Terminalia arjuna stem bark and its flavonoids against CCl4 induced hepatotoxicity in goat liver slice culture. Asian Journal of Plant Science Research., (2016); 6(6): 10-17

De Luca V, Salim V, Atsumi SM, Yu F. Mining the biodiversity of plants: a revolution in the making. Science, (2012); 336(6089): 1658-1661.

Gohar UF, Mukhtar H, Mushtaq A, Farooq A, Saleem F, et al. Fungi: A potential source of biopharmaceuticals. Agrobiological records, (2020); 2: 49-62.

Imran M, Cao S, Wan SF, Chen Z, Saleemi MK, et al. Mycotoxins – a global one health concern: A review. Agrobiological records, (2020); 2: 1-16.

Dhanabal SP, Syamala G, and Suresh B. Hepatoprotective activity of the Indian medicinal plant polygala arvensis on D- galactosamine induced hepatic injury in rats. Fitoterapia., (2006); 77(6): 472-474.

Dwivedi S, Jauhari R, 1997. Beneficial effects of Terminalia arjuna in coronary artery disease. Indian Heart Journal., (1997); 49: 507–510.

Dwivedi S. Terminalia arjuna, Wight and Arn- A useful drug for cardiovascular disorders. Journal of Ethnopharmacology ., (2007); 114:114-129.

Eesha BR, Amberkar VM, Kumari KM, Sarath B, Vijay M, Lalit M,Rajput R. Hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatocellular damage in Wistar albino rats. Asian Pacific Journal of Tropical Medicine , (2011); 4(6): 466-469.

Fahmy NM, Al-Sayed E, Singab AN. Genus terminalia: A phytochemical and Biological Review. Medicinal & Aromatic Plants , (2015); 4: 218.

Foufelle F, Formenty B. 2016. Role of endoplasmic reticulum stress in drug-induced toxicity. Pharmaceutical Research , (2016); 4(1): 211.

Ghosh J, Das J, Manna P, Sil PC. Cytoprotective effect of arjunolic acid in response to sodium fluoride mediated oxidative stress and cell death via necrotic pathway. Toxicology in Vitro, (2008); 22: 1918–1926

Ghosh J, Das J, Manna P, Sil PC. Protective effect of the fruits ofTerminalia arjunaagainst cadmium-induced oxidant stress and hepatic cell injury via MAPK activation and mitochondria dependent pathway. Journal of Food Biochemistry, (2010); 123: 1062–1075.

Goswami HK, Ram HK. Ancient food habits dictate that food can be medicine but medicine cannot be “food”. Medicines ,(2017); 4: 82.

Ilyas U, Katare DP, Aeri V, Naseef PP. A review on hepatoprotective and immunomodulatory herbal plants. Pharmacogenomics , (2016); 10(19): 66-70.

Jaeschke H, McGill MR, Ramachandran A. Oxidant stress, mitochondria, and cell death mechanisms in drug induced liver injury: lessons learned from acetaminophen hepatotoxicity. Drug Metabolism Reviews, (2012); 44: 88–106.

Ji S, Fattahi A, Raffel N, Hoffmann I, Beckmann MW, Dittrich R, Schrauder M. Antioxidant effect of aqueous extract of four plants with therapeutic potential on gynecological diseases; Semen persicae, Leonurus cardiaca, Hedyotis diffusa, and Curcuma zedoaria. European Journal of Medical Research, (2017); 22(1): 50.

Karunakaran G. Cardioprotective role of methanolic extract of bark of Terminalia arjuna against in-vitro model of myocardial ischemic-reperfusion injury.Ancient Science of Life , (2015); 35(2): 79-84.

Mandal S, Patra A, Samanta A, Roy S, Mandal A, Mahapatra TD, Pradhan S, Das K, Nandi DK. Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties. Asian Pacific Journal of Tropical Biomedicine ., (2013); 3(12): 960-966.

Manna P, Sinha M, Sil CP. Aqueous extract of bark of Terminalia arjuna plays a protective role against sodium fluoride induced hepatic and renal oxidative stress. Journal of Natural Medicines ., (2007); 61:251-260.

Michaut A, Moreau C, Robin MA, Fromenty B. Acetaminophen-induced liver injury in obesity and nonalcoholic fatty liver disease. Liver International ., (2014); 34: 171–179.

Montanari F, Pinto M, Khunweeraphong N, Wlcek K, Sohail MI, Noeske T, Boyer S, Chiba P,Stieger B, Kuchler K, Ecker GF. Flagging drugs that inhibit the bile salt export pump. Molecular Pharmacology, (2014); 13(1): 163-171.

Morgan RE, van Staden CJ, Chen Y, Kalyanaraman N, Kalanzi J, Dunn RT, Afshari CA, Hamadeh HK. A multifactorial approach to hepatobiliary transporter assessment enables improved therapeutic compound development. Toxicology Science., (2013); 136(1): 216-241.

Mustafa ZHA, Ali AKA, Qaw FS, Cader ZA. Cimetidine enhances the hepatoprotective action of N- acetylcysteine in mice treated with toxic doses of Paracetamol. Toxicology ,(1997); 121: 223-228.

Owk AK, Lagudu MN. In vitro antimicrobial activity of mature fruits of Terminalia arjuna Wight & Arn. World Journal of Pharmacy and Pharmaceutical Sciences , (2016); 5(5): 766-774.

Raghvan B, Kumari SK. Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats. Indian Journal of Physiological Pharmacolgy, (2006); 50: 133–142.

Rahmat ullah M, Noman A, Shahadat MD, Hossan MD, Rashid HO, Rahman T, Choudhury MH, Jahan R. A survey of medicinal plants in two areas of Dinajpur district, Bangladesh including plants which can be used as functional foods. American-Eurasian Journal of Agricultural & Environmental Sciences,(2009); 3: 862-876.

Rao BK, Sudarshan RP, Rajasekhar MD, Nagaraju N, Rao AC. Antidiabetic activity of Terminalia pallida fruit in alloxan induced diabetic rats. Journal of Ethnopharmacology, (2003); 85:169-172.

Roy A, Bhoumik D, Sahu RK, Dwivedi J. Medicinal plants used in liver protection: A review. UK Journal of Pharmaceutical and Biosciences, (2014); 2: 23-33.

Saleem A, Husheem M, Harkonen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebularetz Fruit. Journal of Ethanopharmacology, (2002); 117: 123-129.

Singh G, Singh AT, Abraham A, Bhat B, Mukherjee A, Verma R, Agarwal SK, Jha S, Mukherjee R, Burman AC. Protective effect of Terminalia arjuna against Doxorubicin- induced cardiotoxicity. Journal of Ethanopharmacology, (2008); 117: 123-129.

Sivalokanathan S, Ilayaraja M, Balasubramanian MP. Efficacy of Terminalia arjuna (Roxb.) on N-nitrosodiethyl amine induced hepatocellular carcinoma in rats. Indian Journal of Experimental Biology, (2005); 43(3): 264-7.

Sohail M I, Mughal MS, Arshad N, Arshad M. Incidence of hepatitis B and C in industrial areas of Sheikhupura and Kasur. Pakistan Journal of Zoology, (2010); 42(6): 673-677.

Subasini, V., Raja manileam, G.V., Dubey, G.P., Prabu, P.C., Sahayam, C.S., Shabi, M.M., Gayathri, K. and Agrawal, A. Hydroalcoholic extract of TA. A potential hepatoprotective herb. Journal of Biological Sciences, (2007); 7: 255-262.

World Health Organization. The World traditional medicines situation in traditional medicines: Global Situation, Issues and Challenges. Geneva, (2011); 3:1–14.

Zahra K, Sohail MI, Malik MA. Effect of Momordica charantia (bitter melon) on serum glucose level and various protein parameters in acetaminophen intoxicated rabbits. Journal of Intercultural Ethnopharmacology, (2012); 1(1): 7-12.


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